##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	A80_CGATGT_L006_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	386824	
Filtered Sequences	0	
Sequence length	101	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.838854362707586	33.0	31.0	34.0	25.0	34.0
2	30.606702272868283	34.0	31.0	34.0	23.0	34.0
3	31.38232891444171	34.0	31.0	34.0	25.0	34.0
4	35.3774584824106	37.0	35.0	37.0	32.0	37.0
5	34.3353075300395	37.0	35.0	37.0	28.0	37.0
6	32.686805885880915	35.0	33.0	37.0	17.0	37.0
7	34.57633704216905	35.0	35.0	37.0	32.0	37.0
8	35.01380989804149	36.0	35.0	37.0	32.0	37.0
9	36.70294242342771	39.0	37.0	39.0	32.0	39.0
10-14	37.86961925837073	39.2	37.6	39.4	34.8	39.4
15-19	39.30590501106447	40.0	39.0	41.0	36.0	41.0
20-24	39.22381289682129	40.0	39.0	41.0	36.0	41.0
25-29	39.070050462225716	40.0	39.0	41.0	36.0	41.0
30-34	38.86757905404008	40.0	38.6	41.0	35.4	41.0
35-39	38.65858633383658	40.0	38.0	41.0	35.0	41.0
40-44	38.37733077575332	40.0	38.0	41.0	34.0	41.0
45-49	38.19309143176225	40.0	38.0	41.0	33.8	41.0
50-54	38.04632080739561	40.0	37.2	41.0	33.6	41.0
55-59	37.51293559861849	39.4	36.2	40.8	33.0	41.0
60-64	36.76491014001199	38.2	35.0	40.0	31.8	41.0
65-69	35.86322255082415	36.6	35.0	39.4	31.0	40.8
70-74	35.181538374040905	35.6	34.4	38.0	31.0	39.6
75-79	34.08660889706947	34.8	33.4	36.2	30.4	38.2
80-84	33.954823382209995	35.0	34.0	35.6	30.8	36.8
85-89	33.56433416747668	35.0	34.0	35.0	30.2	36.0
90-94	33.31702117758981	35.0	34.0	35.0	30.0	35.4
95-99	33.16236479639319	35.0	33.6	35.0	29.8	35.0
100-101	32.8690334622464	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	13.0
10	15.0
11	6.0
12	15.0
13	25.0
14	33.0
15	58.0
16	107.0
17	160.0
18	276.0
19	386.0
20	539.0
21	681.0
22	944.0
23	1191.0
24	1459.0
25	1906.0
26	2342.0
27	2741.0
28	3315.0
29	4022.0
30	4988.0
31	6374.0
32	8347.0
33	11462.0
34	17404.0
35	29805.0
36	58748.0
37	116344.0
38	103967.0
39	9141.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.344294588914138	15.3597179027809	28.685916079179563	42.6100714291254
2	37.75426839988004	25.91857206382693	20.133093413582355	16.19406612271068
3	12.523428270069465	53.559158159242436	13.313203332824914	20.604210237863185
4	15.304376150393978	20.70166277169979	22.862076810125533	41.13188426778069
5	42.3650032055922	12.565404421649124	27.526213471759764	17.543378900998903
6	28.69160134841685	36.00914110810084	34.63616528447046	0.6630922590118503
7	0.3575269373151614	0.28746923665542984	99.047370380328	0.3076334457014043
8	47.94428473931297	11.425351064049801	8.632349595681758	31.99801460095547
9	29.333236820879783	9.410222736955308	26.107738920025646	35.148801522139266
10-14	26.983744545322935	17.96212230885364	25.912559717080637	29.141573428742788
15-19	21.735048099841684	25.455741217371163	32.7938236969922	20.015386985794958
20-24	21.16574900715428	26.96828012390342	31.52314244395418	20.34282842498812
25-29	21.880633414245924	28.009581264408357	29.764646747758526	20.345138573587192
30-34	22.215614936097776	27.169045634399442	29.89587836342464	20.719461066078146
35-39	22.018173523633962	26.988323966230848	29.68114610714393	21.312356402991266
40-44	21.344955138164522	27.00776625918287	29.70252786150654	21.94475074114607
45-49	21.58198437525862	26.94729960110565	29.58619883476505	21.88451718887068
50-54	21.240452655463898	26.988746714968677	29.521912536057116	22.248888093510313
55-59	21.536245377195023	26.311221454987454	29.6270204567202	22.525512711097317
60-64	22.532501087550816	26.200275909443356	29.08315198865326	22.184071014352565
65-69	22.079797722725363	26.793514133131385	28.812355549486135	22.314332594657117
70-74	22.16775770939462	26.086668731710017	28.868116644266635	22.87745691462873
75-79	21.979785778479187	26.778672579459183	27.984638184463822	23.25690345759781
80-84	23.41801865710613	26.152212286886357	28.79550914086518	21.63425991514233
85-89	21.975473471823346	26.430808943144086	28.752690151434457	22.841027433598114
90-94	22.463830319753523	26.141662900338297	29.10623735004928	22.2882694298589
95-99	22.41146897361559	27.19289673177065	28.209761926552613	22.185872368061148
100-101	22.829790644405723	26.738339115732433	28.96400262663971	21.467867613222133
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	55.954366018039536
2	53.94833452259073
3	33.12763850793265
4	56.436260418174676
5	59.908382106591105
6	29.3546936074287
7	0.6651603830165657
8	79.94229934026845
9	64.48203834301904
10-14	56.12531797406572
15-19	41.75043508563664
20-24	41.5085774321424
25-29	42.22577198783311
30-34	42.93507600217592
35-39	43.330529926625225
40-44	43.289705879310596
45-49	43.4665015641293
50-54	43.48934074897421
55-59	44.06175808829234
60-64	44.71657210190338
65-69	44.394130317382476
70-74	45.04521462402335
75-79	45.236689236077
80-84	45.05227857224846
85-89	44.816500905421464
90-94	44.75209974961242
95-99	44.59734134167674
100-101	44.29765825762785
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	6.5
4	7.5
5	4.0
6	3.5
7	7.0
8	7.5
9	3.5
10	3.0
11	3.5
12	6.0
13	6.0
14	6.5
15	11.0
16	20.5
17	46.5
18	135.0
19	267.5
20	303.0
21	280.5
22	353.0
23	475.0
24	602.0
25	687.5
26	754.0
27	994.0
28	1261.5
29	1471.0
30	1930.0
31	2285.0
32	2668.5
33	3342.0
34	4174.5
35	5189.5
36	6358.0
37	8357.0
38	10846.0
39	12624.5
40	14676.0
41	16561.5
42	18437.0
43	21840.0
44	25032.0
45	28166.5
46	29647.5
47	29236.5
48	26480.5
49	20633.0
50	16249.0
51	14198.5
52	12260.0
53	10409.0
54	8371.0
55	6225.5
56	5000.5
57	4669.5
58	3804.0
59	2401.0
60	1665.5
61	1385.0
62	1114.0
63	671.0
64	470.0
65	347.0
66	282.5
67	225.0
68	185.5
69	172.5
70	144.0
71	104.0
72	73.5
73	45.0
74	44.0
75	40.5
76	21.5
77	9.5
78	6.5
79	6.0
80	3.0
81	1.5
82	1.5
83	1.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0018096085041259073
2	0.007238434016503629
3	0.0012925775029470768
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	9.306558021218953E-4
20-24	0.0017062023038901411
25-29	0.017889272640787542
30-34	0.011788306826877341
35-39	0.03919094988935536
40-44	0.03246954687403057
45-49	0.04012160569147726
50-54	0.037691559985936755
55-59	0.039811387090769965
60-64	0.04534361880338345
65-69	0.04818728930986702
70-74	0.017734163340433894
75-79	0.012098525427584638
80-84	0.002998779806837218
85-89	0.011064463425226977
90-94	0.016441585837486814
95-99	0.007083324716149981
100-101	0.004653279010609477
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	386824.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	64.57125619704641
#Duplication Level	Relative count
1	100.0
2	25.88975678377078
3	10.514937252434013
4	4.597045866804872
5	2.199681634768445
6	1.170547514159849
7	0.7781438566616073
8	0.5241920556768963
9	0.3887017362010884
10++	2.013845185651353
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCAGATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA	931	0.24067793104874569	No Hit
CGATGTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA	921	0.23809277604285153	No Hit
TGACCATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA	742	0.1918185014373462	No Hit
ACAGTGTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA	549	0.14192500982358902	No Hit
CGATGTTGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAAT	447	0.11555642876346867	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CAGTG	116000	3.608793	143.40459	2
CGATG	107735	3.3516667	243.53148	1
GACCA	92850	3.3438864	200.3236	2
TCAGA	114165	3.1088393	192.4708	2
TCTTC	144025	3.0243533	8.083573	7
CTCAG	95360	2.9993267	229.81586	1
TGACC	94445	2.970548	171.85858	1
ACCAT	107850	2.969197	154.63335	3
TTCTT	185345	2.9428706	5.9163346	50-54
CAGAT	105215	2.865121	194.19437	3
GCAGC	68545	2.8202276	7.8794107	8
GATGT	119135	2.802457	169.50388	2
CACCA	71420	2.6004171	6.1673083	40-44
ACAGT	94135	2.5634003	130.45032	1
TGTTG	121820	2.5026848	75.091286	4
AGCTT	102000	2.425791	92.4426	2
GATCA	83905	2.284826	116.22873	1
ATCAG	83900	2.28469	116.49133	2
CAACA	71215	2.2449281	5.055074	35-39
TCTTG	106605	2.2142093	6.1829777	7
AGTGT	93885	2.208492	112.645256	3
ATGTT	121010	2.1760561	130.13092	3
TGGAG	67745	2.0846238	5.117205	7
CCATG	66265	2.0842113	86.279724	4
AGATG	76950	2.0726233	89.73939	4
TCAGT	85580	2.035286	102.63138	3
GCGGC	43260	2.0334504	36.75951	8
GGCAG	49490	2.0140595	10.417824	8
TCAGC	61360	1.9299358	6.816372	7
TGGCA	62035	1.9299266	14.721161	7
TTTCT	120310	1.9102579	8.875807	5
CTTTG	90250	1.8745123	43.713856	4
TTGGC	68665	1.8656378	28.40069	6
GCTTT	88200	1.8319336	84.07441	3
TGTTC	87070	1.8084631	45.637222	4
CATCG	57455	1.8071134	15.17291	5
GTTGG	67195	1.8058237	30.801659	5
AGATC	66135	1.8009292	63.939312	4
GATGG	57780	1.7779846	31.344746	5
TAGCT	74230	1.7653577	92.3746	1
CCTTC	63175	1.7544632	5.2645836	9
CCATC	54770	1.7416219	58.02952	4
CGGCA	42150	1.7342271	19.269648	9
TTTGG	81930	1.6831797	14.352903	5
TGGTG	62570	1.6815298	8.273711	4
GGAGG	41755	1.6807758	5.3561435	75-79
ACACC	44485	1.6197083	5.5703588	40-44
AGCGG	39740	1.6172706	8.503943	8
AGTGG	52255	1.6079714	17.828163	5
TGCTG	58820	1.5981477	13.199207	7
TCTGG	58110	1.578857	14.279382	7
TTCTG	75925	1.5769792	8.368168	6
TCCTT	73895	1.551707	5.579091	7
GTTCT	74605	1.5495625	15.819967	5
GCCTT	56045	1.5395095	7.098408	8
ATGGC	49185	1.5301595	32.44387	6
GGTCA	48910	1.5216042	5.590968	60-64
TCTCT	71970	1.5112841	11.616319	7
CTTTC	71450	1.5003648	27.86491	4
CTTTT	94365	1.4983084	5.2962317	4
GTCTT	71940	1.4942098	5.206189	6
CATGG	47405	1.4747832	24.479963	5
GGCGG	31280	1.454321	16.50034	8
TTCTC	68985	1.4486028	12.909284	6
GTGTC	53235	1.4464025	44.484753	4
TGGCG	40350	1.4341227	29.811037	7
TGGCC	39845	1.4317597	39.529293	7
GTTGT	69660	1.4311035	17.15177	5
CAGTC	45360	1.4266932	45.564472	4
GTTCA	59745	1.4208714	5.2347665	5
GCTGG	39510	1.4042673	5.8760486	8
AATAA	66950	1.3816084	5.0601482	20-24
GTTGC	50650	1.3761677	41.507687	5
GTGGC	38635	1.3731679	28.001696	6
ACCGG	33205	1.3661925	5.1664996	8
CGGCG	28930	1.3598641	14.038349	8
AAAGT	56910	1.3417193	10.277786	1
GGCCA	32610	1.3417116	8.457899	8
GTAGC	43125	1.3416312	6.9684186	6
TGGTC	49255	1.3382653	5.4140463	7
GTTGA	56815	1.3364806	6.7858596	5
GATGA	49580	1.3354211	7.0125217	5
TGTCC	48365	1.3285463	23.372805	7
ATCTT	72570	1.3193486	6.637211	6
AGCCT	41695	1.3114191	5.5516253	2
TTGCC	47660	1.3091805	28.63335	6
GATGC	41830	1.3013432	41.810505	5
GTGTG	48380	1.3001823	60.82155	4
TTTCC	61745	1.2965714	10.119738	5
TTGGG	47540	1.2776078	12.247698	6
TTGCT	61040	1.2678144	8.106348	6
CCGGC	26615	1.2648154	16.658167	9
ATGGA	46860	1.262159	5.001483	6
CTCTG	45895	1.2606974	5.739988	8
CATCT	51480	1.2377852	15.713933	5
TTGCA	52020	1.2371534	5.9496465	6
GCGGT	34655	1.2317106	6.5480895	8
CAGTT	51790	1.2316835	8.56703	4
TGCCC	33710	1.2246403	46.8687	7
TGTGG	45530	1.2235904	17.811281	5
ATGGT	51670	1.2154529	14.735009	3
CAGTA	44520	1.2123288	13.202418	4
ATCGG	38780	1.2064569	16.018312	6
TTTGC	57645	1.1972994	19.485443	5
CCCTT	43030	1.1950067	5.22419	9
TTGTC	56340	1.1701941	13.946945	6
GAGAT	43295	1.1661367	5.455077	95-97
CCCAG	27625	1.1491169	6.898503	1
GCCCC	23895	1.1480513	17.027851	8
TGCGG	31845	1.1318372	42.252403	7
GATCT	47570	1.1313223	21.331093	5
TGGCT	41600	1.1302781	13.0016165	7
TGTCT	54125	1.1241883	11.580498	5
ATCTG	47105	1.1202636	10.654017	6
TCTGC	40455	1.111265	8.936268	7
TACCG	35305	1.1104367	7.2890964	7
AGTTT	61340	1.1030434	7.5588574	3
GCCCA	26400	1.0981606	11.414756	8
TGCAG	35295	1.0980375	12.353106	7
GTTTG	53380	1.0966451	5.8069	5
TCCAG	34835	1.0956538	16.072609	9
CCATT	45545	1.0950841	10.922815	4
TGCTT	52670	1.0939676	5.729829	7
GCGGA	26860	1.0931025	10.496747	9
CCATA	39395	1.0845759	17.085081	4
GCTGC	30055	1.0799733	5.01738	8
AGGGG	26820	1.079593	5.2390265	75-79
GCCTC	29600	1.0753294	7.9963894	8
CCGGA	26025	1.0707773	5.645249	9
ATGGG	34330	1.0563899	13.367393	6
GGGCA	25925	1.0550513	8.050137	8
TGTCA	44265	1.052722	6.7567687	7
GGGTC	29480	1.0477803	5.1520386	60-64
CTGGC	29080	1.0449383	6.898897	8
TTTGT	66375	1.0424165	8.657359	5
GATCG	33505	1.04235	17.481377	5
GCCAG	25275	1.0399191	5.465718	8
TGTGC	38250	1.0392579	28.321651	5
TAGCC	32980	1.0373092	6.268537	1
TGCCA	32895	1.0346357	9.378231	7
AGATA	43810	1.0328715	20.163218	4
TCTCC	37035	1.0285167	25.622766	7
TGTTT	65385	1.0268686	9.738577	4
TTGTG	49630	1.0196047	8.565925	6
GTTCC	36995	1.0162219	20.749191	5
CTCCA	31845	1.0126336	16.264914	8
CCCGG	21300	1.0122324	12.626106	8
GGCGA	24860	1.0117098	5.7021804	8
ATGTC	42345	1.00706	16.061739	6
TCGGC	27595	0.9915775	28.536348	7
AGTCC	31445	0.98902917	16.103107	5
TGGGC	27810	0.98842496	27.74322	7
TTAGC	41475	0.98636943	7.1603303	6
GGCCC	20640	0.9808675	21.519678	8
CGGCC	20505	0.97445196	10.759839	9
CCGGT	26820	0.9637294	6.498205	9
CCGCC	20035	0.962595	8.082988	9
GTGTT	46850	0.96249205	8.257153	4
CATGT	40440	0.9617549	14.908709	5
CCTCC	26185	0.96173614	5.146233	9
CCGGG	20455	0.9614941	6.062015	8
TCCGG	26750	0.96121395	21.184492	7
CGCCA	23035	0.9581868	5.364532	9
TGACG	30625	0.95275253	5.414853	7
GTCCC	26195	0.9516302	20.994923	6
GGCCT	26440	0.9500747	10.104445	8
CATCC	29815	0.94808203	18.43871	5
GTGCC	26180	0.9407321	32.891716	6
TGCCG	26150	0.939654	29.111256	7
GGCGT	26435	0.9395547	7.3579845	8
GCCCT	25835	0.93855196	13.386027	8
GATCC	29660	0.9328862	23.361704	5
ATAGC	34010	0.92613	8.027071	6
GTCTG	34000	0.92378485	8.944373	6
AGATT	44855	0.9235742	12.568252	4
CCGTC	25405	0.92293054	5.143052	9
GATAG	34115	0.9188764	8.292274	5
GCCGG	19460	0.91472375	17.889782	8
CGGCT	25370	0.9116261	10.452874	9
GTGCT	33515	0.91060746	9.484462	6
GTGTA	38485	0.90529716	12.020665	4
ATGCC	28780	0.905208	34.49359	6
CGCCC	18795	0.90301836	5.303107	70-74
GATTG	38295	0.9008276	5.998927	5
TCCCC	24450	0.8980121	16.275629	7
CCTCG	24515	0.890598	5.4263225	85-89
GCGCC	18680	0.8877231	13.017792	8
TGCTC	32175	0.8838205	9.708704	7
TTGCG	32320	0.878139	23.368656	6
ATCCT	36520	0.8780869	6.236613	6
GCCTG	24340	0.87461495	6.1497755	8
GTCTC	31760	0.8724207	14.303357	6
TAGCG	28030	0.8720214	12.594436	7
TTTCG	41900	0.8702722	7.3913774	5
GTCCT	31620	0.868575	7.0850887	8
AGTCT	36280	0.86282045	11.2535925	5
TTCGG	31555	0.8573538	12.738157	6
TTCCC	30785	0.854945	16.534292	6
GTGCG	24015	0.85354286	24.744884	6
ATCTC	35320	0.8492341	17.753946	6
CGAGA	23810	0.8481556	6.0283837	95-97
CATGC	26965	0.84812135	37.58917	5
TGTTA	47005	0.845265	15.449015	4
ATGCA	30910	0.84171355	6.5219955	6
CCCGC	17510	0.84127957	10.622024	8
GTCGG	23620	0.8395037	15.439704	6
GGTGC	23560	0.8373712	5.083385	5
TGTGT	40720	0.83655655	10.29903	5
CTTTA	45840	0.8333875	8.849614	4
GTGGG	23530	0.8272011	11.777564	6
CATGA	30305	0.8252387	5.690243	5
TGCCT	29980	0.82352555	18.365295	7
AGTGC	25975	0.8080897	28.220583	5
GCGTG	22505	0.79987437	5.8071213	8
CCCCT	21440	0.7874594	5.6804442	9
GCCGC	16500	0.78412384	10.690719	8
TCCCG	21545	0.78270173	24.640854	7
GATAC	28605	0.7789458	5.1621456	5
CATAG	28580	0.77826506	6.0203032	5
TGCGC	21585	0.7756188	21.881351	7
CCCCG	16040	0.7706525	7.663697	8
TCCTG	28045	0.7703728	5.886484	7
ATGCT	32190	0.7655512	11.253595	6
TGTCG	28135	0.76443195	11.420871	5
GTTCG	28015	0.7611715	14.740432	5
ATGTG	32090	0.75486517	9.9678	6
GTTAC	31635	0.75235194	5.2808876	5
GGGCC	15945	0.7494999	18.208834	8
TCCCT	26960	0.74871904	14.029912	7
TCCCA	23480	0.7466364	6.4597144	7
ATCCG	23470	0.7381943	11.711351	6
ATCCC	22985	0.73089606	20.550837	6
GGCGC	15515	0.7292876	6.4494367	8
GCGCT	20280	0.72872597	6.358832	8
CCCTG	19780	0.7185816	6.6225605	8
TCGCC	19730	0.71676517	11.166762	7
TTCGC	25425	0.6984036	7.69771	6
CGGGC	14855	0.6982641	7.7712293	8
GCCCG	14540	0.6909794	14.008528	8
CCGCA	16045	0.6674238	6.433405	9
GCCGT	18545	0.66638184	6.0975113	8
CCCAC	15780	0.6636245	5.545907	9
TTCCG	24080	0.66145754	10.600999	6
TGCGT	24345	0.6614571	10.511944	7
AGTCG	20860	0.64896053	11.825193	5
TCTCG	23555	0.64703614	7.8175697	7
GTTAG	27450	0.64571667	7.7096486	5
TCCGC	17735	0.6442894	11.360507	7
ATGCG	20465	0.636672	24.962626	6
TGCGA	20350	0.6330943	5.8070154	7
CTCCC	16910	0.62107915	6.517374	8
GTGCA	19630	0.61069494	6.5611734	6
GGCCG	12585	0.59156203	7.6572814	8
TCGCT	19595	0.5382583	6.938593	7
GTCCG	14830	0.53288984	12.264707	6
TACGG	17085	0.53151923	5.1433563	7
CCCGT	14315	0.5200453	5.653835	9
CCCCC	10470	0.5085743	5.3223615	9
ATCGC	16020	0.50387186	10.735406	6
TCGGG	14000	0.497589	11.631474	7
TCGCG	13195	0.474139	9.651487	7
GTCGC	12890	0.46317935	10.243817	6
>>END_MODULE