FastQCFastQC Report
Thu 31 Jul 2014
A18_TGACCA_L006_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename A18_TGACCA_L006_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 339518
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGACC 94530 3.427722 201.17834 1
CAGTG 88135 3.2402408 201.95874 2
GACCA 95270 3.093752 182.52928 2
GAAGA 116375 2.8716707 5.0854006 9
GCTTT 83305 2.822752 140.51575 3
TGGTG 65835 2.7402184 6.47861 7
AAGAA 150010 2.7362444 5.9703493 9
CGATG 74220 2.7286625 158.4144 1
TGTTG 74070 2.544702 34.074223 4
ACCAT 94820 2.5415318 152.55833 3
ACAGT 91935 2.4984431 153.1096 1
AGTGT 78890 2.4272249 175.17673 3
TCAGA 89300 2.4268339 130.87338 2
CTCAG 66630 2.416049 175.23743 1
CTTTG 69695 2.3615832 37.63355 4
CAGAT 86055 2.3386471 132.38918 3
TGCTG 56785 2.3311434 8.101739 7
CAAGA 94100 2.290191 5.9952464 8
TGAAG 80110 2.207335 8.604307 7
GATGT 71730 2.2069316 121.112045 2
AGCTT 72035 2.1859422 123.17595 2
GATCA 79310 2.1553435 111.69514 1
TTGGT 62030 2.1310635 6.0972023 6
TTTGG 61615 2.1168058 11.24633 5
GTGTC 50585 2.0766203 79.93904 4
CCATC 57785 2.066608 70.126915 4
ATCAG 75115 2.0413396 111.51061 2
TCAAG 72905 1.9812803 14.495386 7
ATGTT 77710 1.9734734 99.94167 3
TCAGT 63810 1.9363502 124.61834 3
TTGAA 85095 1.935316 7.4770865 6
TCCAA 70535 1.8906027 6.1735888 7
TAGCT 62035 1.8824868 123.33786 1
TGCCA 51505 1.8676062 10.233104 7
GTTGC 45230 1.8567865 10.511913 5
GTGGT 44055 1.83368 7.6089473 6
GAAAA 99215 1.8097225 5.0150933 9
TGGCC 36515 1.791214 5.470821 7
TTGCC 43875 1.7764769 5.6937094 6
CTTTC 52160 1.7431964 49.355328 4
TTCTT 61510 1.7203375 6.713176 6
ATGGT 55315 1.701888 12.117173 3
CCATG 46920 1.701351 53.339184 4
GTCAA 62380 1.6952507 21.097683 6
TGTTC 49990 1.6938884 44.880882 4
TTGCT 49645 1.6821982 6.309396 6
AGATC 61785 1.679081 45.103127 4
TTGGC 40745 1.6726679 5.3149786 6
GTTGA 54280 1.670044 9.698709 5
TGTCG 40645 1.6685627 17.699036 5
GTTGG 40060 1.6673981 9.810183 5
ATTGG 53520 1.6466612 5.296285 6
TCAAA 81915 1.6455525 16.471775 7
TGGTC 39620 1.626484 5.475277 4
CCATT 54085 1.6187471 23.559101 4
TTGTT 56915 1.613941 5.940179 6
TTTGA 63185 1.6046058 8.731986 5
GTGTT 46665 1.6031932 26.409607 4
TGTCT 46765 1.5846107 16.646524 5
CAAAA 87610 1.5761425 5.635541 8
TTCTG 46370 1.5712264 5.1756763 6
CAAAG 64520 1.5702776 5.062915 8
GTTCT 46155 1.5639411 10.599218 5
TTTTG 54595 1.5481524 7.6875 5
TTCAA 69005 1.5478735 13.81381 6
ATCAA 76935 1.5455116 16.170046 6
TCTTC 46015 1.5378293 8.3619375 7
TGCTT 45345 1.5364946 5.454918 7
AGATG 54735 1.5081571 34.67823 4
TGTCC 37160 1.5045898 12.154736 5
TCAAC 56095 1.5035566 11.567357 7
TTTCT 53620 1.4996667 12.071754 5
GATGA 54025 1.4885942 11.077673 5
TGGAA 53915 1.4855633 8.109961 7
TGGCT 36040 1.4795177 7.4050303 7
CATTG 48725 1.4785873 9.668808 5
ATGTC 48000 1.4565868 5.282561 6
TTGGA 47160 1.4509815 5.1620126 6
CATCA 53895 1.4445884 21.760233 5
GTCGC 29195 1.4321373 10.751511 6
GTGCT 34820 1.4294342 9.8138 6
TGTCA 46990 1.4259378 25.135958 5
TCTTT 50790 1.420516 9.344061 7
GATGG 38020 1.4172089 10.972939 5
TGTTT 49115 1.392756 14.770773 4
TCTGC 34335 1.3902069 5.10463 7
TCCCA 38740 1.3854873 6.5031333 7
AAGTT 60895 1.384935 5.239606 2
GTTGT 40170 1.3800552 7.480893 5
GTCAC 37750 1.3688406 10.602496 6
TGAAA 67165 1.3679943 9.718179 7
CTTTT 48705 1.3622019 16.291048 4
CATGG 36655 1.3476034 13.211725 5
GTTCC 33205 1.3444538 7.461712 5
GTTTG 39085 1.3427795 7.6641655 5
TTTCA 53375 1.3369011 15.718391 5
TTTCC 39690 1.3264468 8.006599 5
TTTGC 38705 1.3115013 9.580379 5
GATTG 42195 1.2982223 8.997417 5
GTTTC 38305 1.2979475 5.1438 4
CATCT 43265 1.2949079 15.28421 5
TCGGT 31515 1.293757 6.4495425 7
TGTGG 30665 1.2763546 14.796016 5
TTCTC 37995 1.2697996 6.919767 6
TCTGG 30885 1.2678941 5.2352767 7
TCGCT 31280 1.2665116 9.090168 7
TTGCA 41480 1.2587337 5.0618405 6
AGTTG 40880 1.2577634 7.132281 5
GTGTG 30140 1.2545027 60.721737 4
GCCAT 34510 1.2513559 5.626614 8
TGAAC 45085 1.2252386 5.218339 7
TGCTC 30255 1.2250098 5.222429 7
GATCG 33320 1.2249936 13.158236 5
AGATT 53845 1.2245969 14.923944 4
GTCCA 33700 1.2219849 6.5935917 6
TCTCT 36445 1.2179983 5.898731 7
ATGGC 32865 1.2082659 5.5086126 6
TCTGA 39735 1.2057807 5.0176225 7
CAGTC 33240 1.205305 51.580555 4
TGCAA 44215 1.2015953 6.6942325 7
TGTTA 46995 1.1934549 28.69778 4
CCCAG 27460 1.189809 5.6110635 1
ATGAA 57975 1.1808155 9.017101 6
CAGTA 43445 1.1806695 29.4317 4
TCTCC 29395 1.173878 5.8673315 7
GTGCC 23575 1.1564527 10.061702 6
GATGC 31295 1.1505455 11.339618 5
CTTTA 45885 1.1492965 27.599916 4
TCACC 32125 1.1489103 5.0290904 7
TTTGT 40280 1.1422216 8.251343 5
ATCTC 38150 1.1418176 7.8660436 6
ATCCA 42595 1.141706 5.6018023 6
GTCGA 30900 1.1360236 10.125864 6
GTTCA 37335 1.1329514 17.601057 5
GCCTT 27900 1.129657 5.616499 3
GTCGT 27490 1.1285223 7.444951 6
GTGGC 22215 1.1048808 7.355697 6
TCGCC 22750 1.1006888 6.756537 7
GTGGA 28870 1.0761395 8.675967 6
TCGTT 31685 1.0736318 5.290613 7
ATGCT 35325 1.0719569 6.592165 6
TCACT 35775 1.0707345 5.645472 7
ATGGA 38260 1.0542085 6.252911 6
GTCTC 26000 1.052727 9.5026045 6
TCCGT 25975 1.0517148 5.0666323 3
GTGAA 38125 1.0504887 10.020691 6
AAAGT 51490 1.0487311 9.750247 1
CAGTT 34350 1.04237 15.865432 4
AGTTT 40945 1.0398129 9.755213 3
CATCC 29055 1.0391155 10.267794 5
ATCGG 28225 1.0376785 5.294686 6
GTCTT 30305 1.0268711 9.726985 6
GTGTA 33200 1.0214713 47.108654 4
CATCG 27970 1.0142112 15.228821 5
GTCTG 24550 1.007829 7.3653255 6
TCTCA 33560 1.0044403 6.922597 7
GTCAT 33025 1.0021621 6.1948686 6
ATCTG 32870 0.99745846 6.0624366 6
GTTAC 32775 0.99457574 5.842492 5
TATTT 47235 0.99011254 5.3971395 7
TTCAG 32515 0.9866858 6.4891615 6
AGTCA 36250 0.98513687 16.250402 5
TCACA 36345 0.9741825 8.084153 7
TAAGA 47630 0.97011197 6.1627474 7
CATGA 35605 0.96760833 12.401971 5
ATCTT 38540 0.9653239 8.441139 6
TCAAT 42385 0.95075166 9.462864 7
TTCAC 31695 0.9486214 7.3871145 6
TTTAT 45235 0.94818956 5.0319724 5
CCATA 35165 0.94255406 40.390102 4
ATGCC 25925 0.94005805 7.9123106 6
TCAGC 25895 0.93897027 8.369343 7
AGATA 45245 0.9215351 27.056482 4
TCGAA 33820 0.91909873 8.288725 7
TAGAA 45120 0.9189891 9.925579 7
TAACA 45440 0.9128231 5.1042047 7
TTTCG 26825 0.9089529 11.716655 5
ATATT 48315 0.9069766 5.8348136 6
GTCCC 18690 0.90425825 6.2639656 6
GTAGA 32720 0.9015604 9.833638 6
TCTCG 22115 0.8954254 6.2826223 7
GTCAG 24335 0.8946645 9.608874 6
CATGC 24615 0.8925566 13.171348 5
TTTAG 35060 0.8903612 8.153137 5
TGGGA 23885 0.89032185 5.6031466 7
GTTAT 34990 0.8885836 5.7761655 5
GTCGG 17710 0.88082105 6.8733563 6
GATCT 28935 0.8780487 13.392109 5
GTAAG 31545 0.8691847 5.611587 6
TATTC 34680 0.8686414 6.024092 7
ATCGA 31925 0.8675999 6.8392873 6
TTCGT 25595 0.86727494 5.0442305 6
TGTGC 20725 0.8508048 16.345228 5
GTCCG 17095 0.8385815 5.185463 6
ATGCA 30705 0.834445 5.1525264 6
GTGGG 16540 0.8340605 6.0641317 6
TCTAA 36855 0.8267064 5.0033536 7
TTTAA 44030 0.8265378 8.594062 5
CATGT 27200 0.8253992 10.625389 5
GTTCG 19875 0.8159105 11.805993 5
GTAAC 29820 0.81039405 5.8114176 6
TAAAG 39360 0.8016713 5.5207944 7
ATCAC 29685 0.79566944 8.253236 6
TTCGC 19415 0.78610367 6.8324513 6
TTCGA 25725 0.78063947 6.518591 6
ATCGT 25590 0.7765429 5.738715 6
AGTCT 25560 0.77563244 10.551805 5
GTTAA 34080 0.7750816 9.529586 5
TTAGA 33985 0.77292097 6.56175 6
GTCTA 25465 0.7727496 6.1507244 6
CGACG 17420 0.7652755 5.1989627 1
TAAAA 50740 0.7639263 8.548824 7
GTATT 30065 0.7635115 6.969886 6
GATAA 37185 0.75737166 11.062969 5
ATCGC 20860 0.7563977 8.351884 6
GTGAT 24520 0.7544119 6.5047326 6
GATAT 33150 0.7539305 6.1104054 5
TGTAG 24450 0.7522583 13.608035 5
AGTCC 20675 0.74968946 6.5065393 5
GATCC 20615 0.74751383 8.124381 5
TGTGT 21300 0.7317694 10.996412 5
TTAAA 43465 0.73071367 6.5949416 6
AGTGG 19580 0.7298514 10.2498455 5
AAGTG 26415 0.72783357 5.693094 2
AGTCG 18995 0.69834197 11.696209 5
AGTGC 18775 0.69025373 10.947367 5
CATAT 30730 0.68931454 7.658434 5
GTTAG 22150 0.6814937 10.041895 5
TAGCC 18720 0.67879987 6.03085 7
TCGAC 18475 0.66991603 5.5385356 7
ATAAA 44135 0.66448337 7.4173636 6
TTAGC 21895 0.664416 6.121296 6
GATAC 24335 0.6613326 5.6144943 5
TAGAG 23985 0.66087794 5.15724 7
GTAAA 32405 0.6600142 9.678816 6
TCGCA 18200 0.65994436 6.6110773 7
CATAC 24240 0.649723 6.629462 5
AGTGA 23180 0.63869715 8.378408 5
TAGCA 23255 0.6319823 5.982641 7
TGTGA 20460 0.62949705 14.473457 5
GTGCA 17045 0.6266512 7.754843 6
TCACG 16475 0.59739465 7.9473596 7
TGTAC 19380 0.588097 7.520185 5
TAAAC 29200 0.5865853 5.454875 7
CATAA 27295 0.5483166 11.096461 5
AGTAA 26530 0.5403542 8.267594 5
ATAGC 19480 0.52939224 5.6532836 6
GTGAG 14140 0.5270735 5.223655 6
GATAG 19090 0.526002 10.022019 5
TGTAA 22450 0.5105804 13.7208395 5
ATAGA 25045 0.5101082 7.861569 6
TGTAT 20070 0.50968486 8.091558 5
GTAGC 13355 0.49099013 8.93144 6
AGTAG 17380 0.47888502 8.53876 5
GTGCG 9420 0.46851125 5.0404615 6
CATAG 16625 0.4518042 11.3607855 5
Produced by FastQC (version 0.10.1)