##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	A18_TGACCA_L006_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	339518	
Filtered Sequences	0	
Sequence length	101	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.015448370925842	33.0	30.0	34.0	16.0	34.0
2	29.701105685118314	33.0	30.0	34.0	16.0	34.0
3	30.444512514800394	34.0	31.0	34.0	23.0	34.0
4	33.73939231498772	37.0	35.0	37.0	26.0	37.0
5	33.14284367839113	37.0	35.0	37.0	19.0	37.0
6	32.65700493051915	37.0	33.0	37.0	17.0	37.0
7	34.05999387366796	36.0	35.0	37.0	28.0	37.0
8	34.78266248033977	37.0	35.0	37.0	32.0	37.0
9	36.77866269240511	39.0	37.0	39.0	33.0	39.0
10-14	37.21695815833034	39.2	37.2	39.4	33.0	39.4
15-19	38.32367002633144	40.0	38.2	41.0	33.0	41.0
20-24	38.263181333537545	40.0	38.0	41.0	33.0	41.0
25-29	38.11208301179908	40.0	38.0	41.0	32.8	41.0
30-34	37.87333572888624	40.0	38.0	41.0	32.0	41.0
35-39	37.64634216742559	40.0	38.0	41.0	31.6	41.0
40-44	37.533399701930385	40.0	38.0	41.0	31.2	41.0
45-49	37.33010208589825	40.0	37.0	41.0	31.0	41.0
50-54	36.675514111181144	39.2	36.0	40.2	30.4	40.6
55-59	37.16847766539624	39.8	36.0	41.0	31.0	41.0
60-64	36.56349766433591	38.8	35.0	40.6	31.0	41.0
65-69	35.67615266348176	36.8	35.0	39.6	30.0	41.0
70-74	34.64645232358815	35.6	34.0	38.0	29.0	39.6
75-79	33.73097567728368	35.0	33.8	36.4	28.8	38.2
80-84	33.010125530899685	35.0	33.0	35.4	27.8	36.6
85-89	32.576414799804425	35.0	33.0	35.0	27.0	35.8
90-94	32.30634782250131	35.0	33.0	35.0	26.4	35.0
95-99	32.0620774156304	35.0	32.2	35.0	25.4	35.0
100-101	31.43048527618565	34.5	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	18.0
7	255.0
8	736.0
9	641.0
10	511.0
11	418.0
12	448.0
13	628.0
14	698.0
15	753.0
16	807.0
17	832.0
18	979.0
19	1021.0
20	1126.0
21	1347.0
22	1415.0
23	1625.0
24	1813.0
25	2121.0
26	2599.0
27	3490.0
28	4276.0
29	4389.0
30	5976.0
31	6570.0
32	6791.0
33	9515.0
34	14483.0
35	23792.0
36	45274.0
37	95053.0
38	92135.0
39	6976.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.739214328850657	21.194856461092044	33.24034113505472	30.82558807500258
2	33.26165149073684	29.875420885339494	16.151392051563793	20.711535572359875
3	14.722120744311018	54.237128345333915	14.519721413672412	16.521029496682655
4	20.127325862292455	17.593476390803787	14.876680689598285	47.402517057305474
5	30.560481709077415	14.297792363120031	35.11097101850597	20.030754909296586
6	34.40258741814905	35.9693539407757	28.691356715534226	0.9367019255410338
7	0.7734858704808808	1.2438807429705863	97.27218101807942	0.7104523684691106
8	25.687043811745575	26.968936488580198	13.656928759521412	33.687090940152814
9	26.319293078055967	33.9979381443299	22.45743740795287	17.22533136966127
10-14	19.30244299894956	33.80272313640839	26.36703250478237	20.52780135985969
15-19	21.129886712008574	31.25598419494938	25.426546050829053	22.187583042212992
20-24	20.73102690078601	30.964449060896914	26.502921385650595	21.80160265266648
25-29	21.183596550499228	31.319789269175462	25.54460298282024	21.952011197505065
30-34	21.205744039798695	30.838628867959432	26.25010162948893	21.705525462752938
35-39	21.54124665755121	30.33827142893331	25.8270627747082	22.29341913880728
40-44	21.809635694986312	29.66622557758906	26.286532721700283	22.237606005724338
45-49	21.63719606136893	29.909664587139535	25.74650585828069	22.706633493210852
50-54	22.22747248668302	29.171215641127013	26.20880956407028	22.392502308119692
55-59	22.302010330356943	29.254201494990966	25.89300550221727	22.55078267243482
60-64	21.909316340580116	29.19172286698509	26.22392186412828	22.67503892830652
65-69	22.198504800863017	28.931791039763766	26.24475711115131	22.624947048221912
70-74	22.367852038116965	28.795229134237747	26.31476307693033	22.52215575071496
75-79	22.72640762889283	28.723778479748425	26.178417997003173	22.37139589435558
80-84	22.494416847861316	28.76646219660945	25.851438066785697	22.88768288874354
85-89	22.255892236053572	28.93917066493989	26.141842609346856	22.66309448965968
90-94	22.411193044302262	29.103824537645167	26.294189854388357	22.19079256366421
95-99	22.708563273017194	29.626229299535712	25.699659929888163	21.965547497558934
100-101	22.883921560539253	29.117062542078493	25.779363603819146	22.219652293563115
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	45.564802403853236
2	53.97318706309672
3	31.24315024099367
4	67.52984291959793
5	50.59123661837399
6	35.339289343690076
7	1.4839382389499916
8	59.37413475189839
9	43.54462444771723
10-14	39.830244358809246
15-19	43.31746975422156
20-24	42.53262955345249
25-29	43.1356077480043
30-34	42.91126950255163
35-39	43.83466579635849
40-44	44.04724170071066
45-49	44.343829554579784
50-54	44.61997479480271
55-59	44.85279300279176
60-64	44.58435526888664
65-69	44.823451849084925
70-74	44.89000778883192
75-79	45.09780352324841
80-84	45.38209973660485
85-89	44.91898672571325
90-94	44.601985607966476
95-99	44.67411077057613
100-101	45.10357385410237
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	3.0
2	3.5
3	8.5
4	11.5
5	8.5
6	8.5
7	11.0
8	14.0
9	13.5
10	13.5
11	11.5
12	10.0
13	13.5
14	18.0
15	25.5
16	27.5
17	23.0
18	28.5
19	42.0
20	53.5
21	84.0
22	137.5
23	195.0
24	257.0
25	355.5
26	475.5
27	682.5
28	994.5
29	1364.5
30	1724.5
31	2264.5
32	3090.0
33	4111.5
34	5116.5
35	6190.5
36	7717.0
37	9600.0
38	11906.0
39	14199.0
40	16240.0
41	18321.0
42	20507.5
43	22287.0
44	22933.0
45	22717.5
46	22497.5
47	21484.0
48	19628.0
49	16641.5
50	12407.0
51	9433.0
52	8280.0
53	7442.0
54	6352.5
55	5170.0
56	3748.0
57	2920.0
58	2437.0
59	1818.0
60	1355.0
61	933.5
62	670.0
63	501.5
64	388.5
65	303.0
66	253.5
67	238.0
68	191.5
69	142.5
70	110.0
71	88.0
72	75.0
73	52.5
74	37.0
75	29.5
76	18.0
77	11.0
78	10.0
79	9.0
80	4.0
81	2.5
82	2.0
83	1.5
84	1.5
85	2.5
86	2.0
87	0.5
88	1.0
89	1.5
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.018555717222650935
2	0.016199435670568276
3	0.026508167460929905
4	0.02179560435676459
5	0.017672111640619936
6	0.008541520626299638
7	0.004712563104165317
8	0.005890703880206646
9	0.005301633492185981
10-14	0.01231157110963189
15-19	0.024210792947649315
20-24	0.01643506382577654
25-29	0.0040645856773425856
30-34	0.01460894562291248
35-39	0.0063030531518211115
40-44	0.016258342709370342
45-49	0.014667852661714548
50-54	0.018909159455463335
55-59	0.026861609693742305
60-64	0.014078782273693885
65-69	0.01714194829140134
70-74	0.01696522717499514
75-79	0.02727395896535677
80-84	0.030631660177074558
85-89	0.023503908482024514
90-94	0.03999787934660313
95-99	0.034578431776813016
100-101	0.03608056126626571
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	339518.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	57.31402295163872
#Duplication Level	Relative count
1	100.0
2	25.969597133113695
3	10.097682214900873
4	3.9795387549852608
5	1.7462863418299521
6	0.8431593549505809
7	0.47685104907230796
8	0.29405814692792326
9	0.20302294665048262
10++	0.8901219582683082
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGACC	94530	3.427722	201.17834	1
CAGTG	88135	3.2402408	201.95874	2
GACCA	95270	3.093752	182.52928	2
GAAGA	116375	2.8716707	5.0854006	9
GCTTT	83305	2.822752	140.51575	3
TGGTG	65835	2.7402184	6.47861	7
AAGAA	150010	2.7362444	5.9703493	9
CGATG	74220	2.7286625	158.4144	1
TGTTG	74070	2.544702	34.074223	4
ACCAT	94820	2.5415318	152.55833	3
ACAGT	91935	2.4984431	153.1096	1
AGTGT	78890	2.4272249	175.17673	3
TCAGA	89300	2.4268339	130.87338	2
CTCAG	66630	2.416049	175.23743	1
CTTTG	69695	2.3615832	37.63355	4
CAGAT	86055	2.3386471	132.38918	3
TGCTG	56785	2.3311434	8.101739	7
CAAGA	94100	2.290191	5.9952464	8
TGAAG	80110	2.207335	8.604307	7
GATGT	71730	2.2069316	121.112045	2
AGCTT	72035	2.1859422	123.17595	2
GATCA	79310	2.1553435	111.69514	1
TTGGT	62030	2.1310635	6.0972023	6
TTTGG	61615	2.1168058	11.24633	5
GTGTC	50585	2.0766203	79.93904	4
CCATC	57785	2.066608	70.126915	4
ATCAG	75115	2.0413396	111.51061	2
TCAAG	72905	1.9812803	14.495386	7
ATGTT	77710	1.9734734	99.94167	3
TCAGT	63810	1.9363502	124.61834	3
TTGAA	85095	1.935316	7.4770865	6
TCCAA	70535	1.8906027	6.1735888	7
TAGCT	62035	1.8824868	123.33786	1
TGCCA	51505	1.8676062	10.233104	7
GTTGC	45230	1.8567865	10.511913	5
GTGGT	44055	1.83368	7.6089473	6
GAAAA	99215	1.8097225	5.0150933	9
TGGCC	36515	1.791214	5.470821	7
TTGCC	43875	1.7764769	5.6937094	6
CTTTC	52160	1.7431964	49.355328	4
TTCTT	61510	1.7203375	6.713176	6
ATGGT	55315	1.701888	12.117173	3
CCATG	46920	1.701351	53.339184	4
GTCAA	62380	1.6952507	21.097683	6
TGTTC	49990	1.6938884	44.880882	4
TTGCT	49645	1.6821982	6.309396	6
AGATC	61785	1.679081	45.103127	4
TTGGC	40745	1.6726679	5.3149786	6
GTTGA	54280	1.670044	9.698709	5
TGTCG	40645	1.6685627	17.699036	5
GTTGG	40060	1.6673981	9.810183	5
ATTGG	53520	1.6466612	5.296285	6
TCAAA	81915	1.6455525	16.471775	7
TGGTC	39620	1.626484	5.475277	4
CCATT	54085	1.6187471	23.559101	4
TTGTT	56915	1.613941	5.940179	6
TTTGA	63185	1.6046058	8.731986	5
GTGTT	46665	1.6031932	26.409607	4
TGTCT	46765	1.5846107	16.646524	5
CAAAA	87610	1.5761425	5.635541	8
TTCTG	46370	1.5712264	5.1756763	6
CAAAG	64520	1.5702776	5.062915	8
GTTCT	46155	1.5639411	10.599218	5
TTTTG	54595	1.5481524	7.6875	5
TTCAA	69005	1.5478735	13.81381	6
ATCAA	76935	1.5455116	16.170046	6
TCTTC	46015	1.5378293	8.3619375	7
TGCTT	45345	1.5364946	5.454918	7
AGATG	54735	1.5081571	34.67823	4
TGTCC	37160	1.5045898	12.154736	5
TCAAC	56095	1.5035566	11.567357	7
TTTCT	53620	1.4996667	12.071754	5
GATGA	54025	1.4885942	11.077673	5
TGGAA	53915	1.4855633	8.109961	7
TGGCT	36040	1.4795177	7.4050303	7
CATTG	48725	1.4785873	9.668808	5
ATGTC	48000	1.4565868	5.282561	6
TTGGA	47160	1.4509815	5.1620126	6
CATCA	53895	1.4445884	21.760233	5
GTCGC	29195	1.4321373	10.751511	6
GTGCT	34820	1.4294342	9.8138	6
TGTCA	46990	1.4259378	25.135958	5
TCTTT	50790	1.420516	9.344061	7
GATGG	38020	1.4172089	10.972939	5
TGTTT	49115	1.392756	14.770773	4
TCTGC	34335	1.3902069	5.10463	7
TCCCA	38740	1.3854873	6.5031333	7
AAGTT	60895	1.384935	5.239606	2
GTTGT	40170	1.3800552	7.480893	5
GTCAC	37750	1.3688406	10.602496	6
TGAAA	67165	1.3679943	9.718179	7
CTTTT	48705	1.3622019	16.291048	4
CATGG	36655	1.3476034	13.211725	5
GTTCC	33205	1.3444538	7.461712	5
GTTTG	39085	1.3427795	7.6641655	5
TTTCA	53375	1.3369011	15.718391	5
TTTCC	39690	1.3264468	8.006599	5
TTTGC	38705	1.3115013	9.580379	5
GATTG	42195	1.2982223	8.997417	5
GTTTC	38305	1.2979475	5.1438	4
CATCT	43265	1.2949079	15.28421	5
TCGGT	31515	1.293757	6.4495425	7
TGTGG	30665	1.2763546	14.796016	5
TTCTC	37995	1.2697996	6.919767	6
TCTGG	30885	1.2678941	5.2352767	7
TCGCT	31280	1.2665116	9.090168	7
TTGCA	41480	1.2587337	5.0618405	6
AGTTG	40880	1.2577634	7.132281	5
GTGTG	30140	1.2545027	60.721737	4
GCCAT	34510	1.2513559	5.626614	8
TGAAC	45085	1.2252386	5.218339	7
TGCTC	30255	1.2250098	5.222429	7
GATCG	33320	1.2249936	13.158236	5
AGATT	53845	1.2245969	14.923944	4
GTCCA	33700	1.2219849	6.5935917	6
TCTCT	36445	1.2179983	5.898731	7
ATGGC	32865	1.2082659	5.5086126	6
TCTGA	39735	1.2057807	5.0176225	7
CAGTC	33240	1.205305	51.580555	4
TGCAA	44215	1.2015953	6.6942325	7
TGTTA	46995	1.1934549	28.69778	4
CCCAG	27460	1.189809	5.6110635	1
ATGAA	57975	1.1808155	9.017101	6
CAGTA	43445	1.1806695	29.4317	4
TCTCC	29395	1.173878	5.8673315	7
GTGCC	23575	1.1564527	10.061702	6
GATGC	31295	1.1505455	11.339618	5
CTTTA	45885	1.1492965	27.599916	4
TCACC	32125	1.1489103	5.0290904	7
TTTGT	40280	1.1422216	8.251343	5
ATCTC	38150	1.1418176	7.8660436	6
ATCCA	42595	1.141706	5.6018023	6
GTCGA	30900	1.1360236	10.125864	6
GTTCA	37335	1.1329514	17.601057	5
GCCTT	27900	1.129657	5.616499	3
GTCGT	27490	1.1285223	7.444951	6
GTGGC	22215	1.1048808	7.355697	6
TCGCC	22750	1.1006888	6.756537	7
GTGGA	28870	1.0761395	8.675967	6
TCGTT	31685	1.0736318	5.290613	7
ATGCT	35325	1.0719569	6.592165	6
TCACT	35775	1.0707345	5.645472	7
ATGGA	38260	1.0542085	6.252911	6
GTCTC	26000	1.052727	9.5026045	6
TCCGT	25975	1.0517148	5.0666323	3
GTGAA	38125	1.0504887	10.020691	6
AAAGT	51490	1.0487311	9.750247	1
CAGTT	34350	1.04237	15.865432	4
AGTTT	40945	1.0398129	9.755213	3
CATCC	29055	1.0391155	10.267794	5
ATCGG	28225	1.0376785	5.294686	6
GTCTT	30305	1.0268711	9.726985	6
GTGTA	33200	1.0214713	47.108654	4
CATCG	27970	1.0142112	15.228821	5
GTCTG	24550	1.007829	7.3653255	6
TCTCA	33560	1.0044403	6.922597	7
GTCAT	33025	1.0021621	6.1948686	6
ATCTG	32870	0.99745846	6.0624366	6
GTTAC	32775	0.99457574	5.842492	5
TATTT	47235	0.99011254	5.3971395	7
TTCAG	32515	0.9866858	6.4891615	6
AGTCA	36250	0.98513687	16.250402	5
TCACA	36345	0.9741825	8.084153	7
TAAGA	47630	0.97011197	6.1627474	7
CATGA	35605	0.96760833	12.401971	5
ATCTT	38540	0.9653239	8.441139	6
TCAAT	42385	0.95075166	9.462864	7
TTCAC	31695	0.9486214	7.3871145	6
TTTAT	45235	0.94818956	5.0319724	5
CCATA	35165	0.94255406	40.390102	4
ATGCC	25925	0.94005805	7.9123106	6
TCAGC	25895	0.93897027	8.369343	7
AGATA	45245	0.9215351	27.056482	4
TCGAA	33820	0.91909873	8.288725	7
TAGAA	45120	0.9189891	9.925579	7
TAACA	45440	0.9128231	5.1042047	7
TTTCG	26825	0.9089529	11.716655	5
ATATT	48315	0.9069766	5.8348136	6
GTCCC	18690	0.90425825	6.2639656	6
GTAGA	32720	0.9015604	9.833638	6
TCTCG	22115	0.8954254	6.2826223	7
GTCAG	24335	0.8946645	9.608874	6
CATGC	24615	0.8925566	13.171348	5
TTTAG	35060	0.8903612	8.153137	5
TGGGA	23885	0.89032185	5.6031466	7
GTTAT	34990	0.8885836	5.7761655	5
GTCGG	17710	0.88082105	6.8733563	6
GATCT	28935	0.8780487	13.392109	5
GTAAG	31545	0.8691847	5.611587	6
TATTC	34680	0.8686414	6.024092	7
ATCGA	31925	0.8675999	6.8392873	6
TTCGT	25595	0.86727494	5.0442305	6
TGTGC	20725	0.8508048	16.345228	5
GTCCG	17095	0.8385815	5.185463	6
ATGCA	30705	0.834445	5.1525264	6
GTGGG	16540	0.8340605	6.0641317	6
TCTAA	36855	0.8267064	5.0033536	7
TTTAA	44030	0.8265378	8.594062	5
CATGT	27200	0.8253992	10.625389	5
GTTCG	19875	0.8159105	11.805993	5
GTAAC	29820	0.81039405	5.8114176	6
TAAAG	39360	0.8016713	5.5207944	7
ATCAC	29685	0.79566944	8.253236	6
TTCGC	19415	0.78610367	6.8324513	6
TTCGA	25725	0.78063947	6.518591	6
ATCGT	25590	0.7765429	5.738715	6
AGTCT	25560	0.77563244	10.551805	5
GTTAA	34080	0.7750816	9.529586	5
TTAGA	33985	0.77292097	6.56175	6
GTCTA	25465	0.7727496	6.1507244	6
CGACG	17420	0.7652755	5.1989627	1
TAAAA	50740	0.7639263	8.548824	7
GTATT	30065	0.7635115	6.969886	6
GATAA	37185	0.75737166	11.062969	5
ATCGC	20860	0.7563977	8.351884	6
GTGAT	24520	0.7544119	6.5047326	6
GATAT	33150	0.7539305	6.1104054	5
TGTAG	24450	0.7522583	13.608035	5
AGTCC	20675	0.74968946	6.5065393	5
GATCC	20615	0.74751383	8.124381	5
TGTGT	21300	0.7317694	10.996412	5
TTAAA	43465	0.73071367	6.5949416	6
AGTGG	19580	0.7298514	10.2498455	5
AAGTG	26415	0.72783357	5.693094	2
AGTCG	18995	0.69834197	11.696209	5
AGTGC	18775	0.69025373	10.947367	5
CATAT	30730	0.68931454	7.658434	5
GTTAG	22150	0.6814937	10.041895	5
TAGCC	18720	0.67879987	6.03085	7
TCGAC	18475	0.66991603	5.5385356	7
ATAAA	44135	0.66448337	7.4173636	6
TTAGC	21895	0.664416	6.121296	6
GATAC	24335	0.6613326	5.6144943	5
TAGAG	23985	0.66087794	5.15724	7
GTAAA	32405	0.6600142	9.678816	6
TCGCA	18200	0.65994436	6.6110773	7
CATAC	24240	0.649723	6.629462	5
AGTGA	23180	0.63869715	8.378408	5
TAGCA	23255	0.6319823	5.982641	7
TGTGA	20460	0.62949705	14.473457	5
GTGCA	17045	0.6266512	7.754843	6
TCACG	16475	0.59739465	7.9473596	7
TGTAC	19380	0.588097	7.520185	5
TAAAC	29200	0.5865853	5.454875	7
CATAA	27295	0.5483166	11.096461	5
AGTAA	26530	0.5403542	8.267594	5
ATAGC	19480	0.52939224	5.6532836	6
GTGAG	14140	0.5270735	5.223655	6
GATAG	19090	0.526002	10.022019	5
TGTAA	22450	0.5105804	13.7208395	5
ATAGA	25045	0.5101082	7.861569	6
TGTAT	20070	0.50968486	8.091558	5
GTAGC	13355	0.49099013	8.93144	6
AGTAG	17380	0.47888502	8.53876	5
GTGCG	9420	0.46851125	5.0404615	6
CATAG	16625	0.4518042	11.3607855	5
>>END_MODULE