FastQCFastQC Report
Thu 31 Jul 2014
A18_TGACCA_L006_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename A18_TGACCA_L006_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 339518
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TGACCATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 765 0.2253194234179042 No Hit
ACAGTGTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 698 0.20558556541921194 No Hit
CTCAGATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 610 0.1796664683463027 No Hit
CGATGTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 485 0.14284956909501118 No Hit
GATCAGTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 356 0.1048545290676783 No Hit
ACAGTGTGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAAT 340 0.10014196596351299 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CAGTG 118675 4.1994867 194.83119 2
GACCA 90835 3.699827 233.38332 2
ACCAT 105345 3.2945564 181.16765 3
TGACC 92025 3.2933114 202.24902 1
TCTTC 128030 3.0909517 8.3808775 7
ACAGT 98220 3.0373368 177.08838 1
TTCTT 164045 3.0068257 6.1629214 50-54
GCAGC 60240 2.8077416 8.044854 8
CACCA 64685 2.6645374 5.9863844 40-44
AGTGT 98740 2.652741 153.66527 3
TCAGA 85700 2.650171 147.16948 2
AGCTT 95240 2.5876832 107.02766 2
CTCAG 68590 2.4546397 176.42776 1
CAGAT 78795 2.436642 148.52301 3
CGATG 68805 2.4347644 157.10497 1
ATCAG 76730 2.3727844 123.67334 2
GATCA 75515 2.335212 123.69197 1
TGTTG 95970 2.2653532 49.53175 4
CAACA 62745 2.2333841 5.072747 35-39
TCTTG 93460 2.2310855 6.4120398 7
CCATG 61980 2.2180867 100.28842 4
GATGT 78990 2.122139 110.33658 2
TCAGT 77565 2.1074514 109.40415 3
GCGGC 38445 2.0504818 36.20263 8
GGCAG 44040 2.029689 10.636167 8
TGGCA 55910 1.9784561 15.355217 7
GCTTT 82140 1.9608535 98.219086 3
CTTTG 81715 1.9507079 51.215298 4
TTTCT 105845 1.9400618 10.148628 5
TAGCT 70845 1.9248681 106.80685 1
TCAGC 53505 1.9147907 6.0554776 7
AGATG 61710 1.8869419 68.99623 4
TTGGC 59685 1.8556706 25.430025 6
CCATC 51110 1.8497918 68.59281 4
CCTTC 56020 1.781389 5.812367 9
CATCG 49725 1.7795154 17.281534 5
GTTGG 56310 1.7311361 20.226538 5
CGGCA 36850 1.7175511 18.372095 9
TTTGG 72195 1.7041488 17.201067 5
ATGTT 82510 1.702014 85.131546 3
GATGG 48285 1.6895038 24.293268 5
TGTTC 69425 1.6573199 30.33566 4
GGAGG 36335 1.655836 5.3656206 75-79
CATCA 52665 1.6470437 5.4434404 5
TGGTG 53500 1.6447484 5.336109 4
ACACC 39470 1.6258684 5.3509808 40-44
TGCTG 52225 1.6237313 13.1596985 7
AGTGG 46170 1.6154995 19.25479 5
GTGTC 51885 1.6131603 60.492405 4
AGCGG 34940 1.6102935 8.625126 8
AGATC 51835 1.6029358 48.502403 4
TTCTG 66705 1.5923878 7.314818 6
GCCTT 50230 1.579388 8.003519 8
TCTGG 50715 1.5767837 14.214887 7
GTGTG 51150 1.5725025 82.00914 4
ATGGC 43690 1.5460339 32.100124 6
CTTTC 63985 1.5447516 32.01355 4
CATGG 43430 1.5368334 27.931055 5
GGTCA 42995 1.5214401 5.65402 60-64
TCTCT 62775 1.5155392 11.833892 7
GTTCT 63045 1.505016 10.347226 5
CTTTT 81810 1.4995179 6.354001 4
GTCTT 62350 1.4884248 6.435188 6
CAGTC 41550 1.4869555 49.085804 4
TGGCG 35810 1.4500574 30.430464 7
GTGGC 35600 1.4415538 32.39372 6
TTCTC 58820 1.4200561 10.932597 6
GCTGG 35065 1.41989 6.046828 8
GGCGG 26915 1.4194511 15.8786545 8
GTTTC 59245 1.414302 5.3819466 4
TGGCC 34375 1.4077113 39.233208 7
GTAGC 39440 1.3956414 8.13226 6
TGTCC 44105 1.386799 22.211672 7
AATAA 59040 1.3786739 5.107646 20-24
GTTGT 58170 1.3730918 11.284267 5
CGGCG 25660 1.368588 13.98973 8
AGCCT 37820 1.3534695 6.6112914 2
AAAGT 50530 1.3502319 13.44942 1
GGCCA 28445 1.3258003 7.5702977 8
TTTCC 54860 1.324452 12.536201 5
TGGTC 42525 1.3221478 5.17042 7
ATCTT 63320 1.3209532 6.119246 6
GATGA 42735 1.3067325 6.4044633 5
TGTGG 41745 1.2833649 23.05855 5
TTGCT 53225 1.270592 7.511577 6
TTGCC 40385 1.2698305 25.504547 6
CATCT 46125 1.2674129 18.464647 5
TTGGG 40810 1.2546202 10.597692 6
CTCTG 39740 1.2495497 5.9759607 8
CAGTT 45965 1.2488751 9.458281 4
TGCCC 30135 1.2480503 48.87395 7
GTTGC 40080 1.2461301 27.660994 5
GCGGT 30530 1.2362539 6.321683 9
TTTGC 51515 1.2297705 23.58797 5
TTGCA 45035 1.223607 5.4404874 6
CCGGC 22615 1.2198395 17.024843 9
AGTAG 39885 1.2195866 5.203627 5
TGTCT 49930 1.1919335 16.226858 5
CAGTA 38490 1.1902577 13.943507 4
GATGC 33415 1.1824381 31.980028 5
CCCTT 37055 1.1783179 5.6581926 8
AAGTT 49710 1.1670827 6.340849 2
TGGCT 37425 1.1635833 12.210029 7
ATGGT 43195 1.1604735 9.53488 3
TTGTC 48555 1.1591094 11.388894 6
ATCGG 32370 1.1454593 14.840518 6
TCTGC 36125 1.1358829 7.988274 7
GCCCA 24095 1.1357666 12.181068 8
TGCGG 28020 1.1346163 41.012413 7
CCCAG 24060 1.1341168 6.0341105 1
CCATT 41210 1.1323596 13.655313 4
TGTGC 36240 1.1267405 38.484203 5
CCATA 35965 1.1247684 19.650972 4
AAGTG 36705 1.12235 5.69336 2
AGTTT 54290 1.1198926 10.491424 3
TGCTT 46475 1.1094553 5.4745393 7
TACCG 30955 1.1077908 7.356798 7
GTTTG 46830 1.1054131 6.6263595 4
GCCCC 20215 1.1027317 17.058931 8
GCCTC 26590 1.101233 6.726694 8
TGCAG 30830 1.0909641 11.597907 7
ATCTG 40000 1.0868052 8.654721 6
TCCAG 30240 1.082203 15.667896 9
TGCCA 30065 1.0759403 9.352156 7
ATTGC 39170 1.0642542 5.7039495 6
AGGGG 23335 1.063408 5.299324 75-79
TAGCC 29655 1.0612676 7.5485477 1
GCGGA 23020 1.0609318 9.451886 9
CTGGC 25870 1.059418 6.8697824 8
ATGGG 30275 1.0593295 12.638606 6
TTTGT 58355 1.0576289 9.727446 5
TGTAG 39245 1.0543532 5.6010084 5
TGTCA 38575 1.0480878 5.796161 7
GATCT 38540 1.0471369 15.596942 5
CCGGA 22345 1.0414839 5.6946716 9
GGCGA 22425 1.0335097 6.010774 8
TGTTT 56820 1.0298085 6.168625 4
GGGCA 22265 1.0261357 6.859879 8
GTGTT 43405 1.0245668 12.199827 4
ATGTC 37670 1.0234989 14.60896 6
AGTCC 28545 1.0215439 16.518093 5
GTGCC 24850 1.0176473 40.38479 6
CCCGG 18720 1.0097455 12.3175125 8
TTGTG 42515 1.0035584 7.576252 6
CATTG 36610 0.9946985 6.467988 5
TCTCC 31050 0.98736393 25.392488 7
CATCC 27255 0.9864229 22.00445 5
GGCCC 18270 0.9854728 22.072145 8
AGATA 36825 0.9840152 15.391166 4
CTCCA 27180 0.9837085 15.353983 8
CATGT 36110 0.9811135 16.848387 5
GTCCC 23610 0.9778154 24.15586 6
TCCGG 23790 0.9742386 21.939623 7
TGACG 27450 0.97135794 5.781797 7
TTAGC 35620 0.96780014 6.1650076 6
CCGCC 17730 0.96717453 8.357554 9
TCGGC 23515 0.9629769 27.002613 7
GTCTG 30955 0.96242416 11.109619 6
CGGCC 17775 0.9587728 10.513037 9
GCCCT 23150 0.9587644 13.493547 8
GGCGT 23665 0.9582688 7.8530226 8
GGCCT 23345 0.95601505 11.1385765 8
CCGGG 17805 0.9496379 6.8267813 8
GATCG 26745 0.94641054 12.936128 5
CCGGT 23080 0.94516283 5.936603 9
ATAGC 30515 0.94364023 7.901321 6
GTTCC 29995 0.94313645 13.537381 5
CATGC 26330 0.94227535 44.3837 5
TGGGC 23180 0.93862975 26.287994 7
GTGTA 34930 0.93842673 16.451334 4
GTGCT 30065 0.934753 10.973951 6
TGCCG 22740 0.9312394 29.047663 7
GATAG 30375 0.9287939 6.374814 5
CCGTC 22415 0.9283241 5.2407975 9
GATTG 34440 0.92526245 5.027882 5
TGGGG 23040 0.9225148 5.2220273 7
GTCTC 29165 0.9170387 16.891237 6
ATGCC 25590 0.9157928 34.04254 6
AGATT 38945 0.9143438 9.6413355 4
TGCTC 28965 0.91075003 9.909119 7
CGGCT 22210 0.909535 11.019447 9
CGCCC 16545 0.9025326 5.3175426 70-74
TCCCC 21450 0.8984175 16.4081 7
CCTCG 21595 0.8943636 5.483498 85-89
GATCC 24870 0.8900261 18.773716 5
TGTGT 37665 0.88907516 14.397169 5
GTCCT 28265 0.8887399 6.6162424 6
GCGCC 16475 0.88865155 12.657487 8
GCCTG 21555 0.88271165 7.3661532 8
AGTCT 32330 0.8784104 12.237803 5
TTGCG 28230 0.877701 19.686789 6
TAGCG 24765 0.87634534 12.455741 7
GTACC 24330 0.8707011 5.6911077 6
GTCGG 21425 0.8675644 18.160116 6
TTTCG 36270 0.8658406 8.784725 5
GTGCG 21335 0.86392 26.09167 6
ATCCT 31230 0.8581313 5.901759 6
GCCGG 16040 0.8555008 17.506556 8
CATGA 27660 0.8553527 6.986747 5
AGTGC 23945 0.8473285 29.046238 5
CCCGC 15400 0.84007263 11.108142 8
GCCGC 15570 0.8398364 11.114529 8
ATCTC 30560 0.8397212 17.53209 6
ATGCA 27145 0.83942693 6.177124 6
CTTTA 40220 0.83905154 10.266172 4
TTCCC 26375 0.83870286 14.384452 6
TTCGG 26890 0.83603895 11.079472 6
TGCCT 26480 0.8326139 19.34565 7
GTGGG 20415 0.8174105 12.812408 6
GCGTG 20150 0.8159357 5.634544 8
TGTTA 38815 0.80067474 9.741178 4
CGCTT 25330 0.7964543 5.7935348 2
CATAG 25690 0.7944328 7.136677 5
CGAGA 19710 0.79382515 5.513865 95-97
TCCCG 19085 0.7904111 24.718092 7
ATGCT 28865 0.7842659 10.696551 6
TCCTG 24860 0.78167605 6.814425 7
TGTCG 25105 0.78054136 15.285149 5
TGCGC 18625 0.76272357 21.105719 7
CCCCG 13850 0.75551987 7.775699 8
ATCCG 21060 0.7536771 11.330163 6
GTTCG 24180 0.7517821 10.883508 5
TCCCA 20630 0.7466485 6.8961315 7
TCGGT 24000 0.74618566 5.396531 7
GGGCC 13985 0.74589646 16.911798 8
CCCCT 17765 0.7440739 5.8281245 9
GGCGC 13940 0.74349636 7.0853715 8
ATCCC 20485 0.7414006 20.530468 6
TCCCT 23110 0.73487866 13.675252 7
ATGTG 27295 0.7333054 8.727153 6
GCGCT 17660 0.7232052 6.135152 8
CCCTG 17215 0.71296453 6.084144 8
CGGGC 13315 0.7101617 7.809424 8
TCGCC 17115 0.70882297 12.14821 7
GCCCG 13100 0.7066061 15.351125 8
CCGCA 14635 0.68985033 6.741867 9
TTCGC 21640 0.68042916 7.6071544 6
AGTCG 19025 0.67322713 13.244947 5
CCCAC 13945 0.6647688 5.50079 9
GCCGT 16090 0.65891117 5.976313 8
TCCGC 15820 0.65519017 10.260719 7
TGCGT 21010 0.6532234 9.3158 7
TGCAC 18135 0.64899975 5.222632 7
TTCCG 20580 0.64709944 9.345061 6
TCTCG 20385 0.6409681 7.5156856 7
GTGCA 18045 0.6385484 7.7376566 6
ATGCG 17825 0.6307634 24.139431 6
GGCCG 11260 0.6005573 6.2578826 8
CTCCC 14255 0.5970602 6.3358006 8
GTCCG 14160 0.5798747 14.652888 6
TCGCT 17660 0.5552856 8.079743 7
CCCGT 12610 0.522247 6.606216 9
TCGGG 12880 0.52155095 11.759902 7
CCCCC 9325 0.5144403 5.349487 9
ATCGC 13860 0.49600974 10.774933 6
GTCGC 11950 0.48937163 12.885805 6
TCGCG 11755 0.48138607 9.292075 7
GACTA 14410 0.44561213 5.5629005 2
Produced by FastQC (version 0.10.1)