##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL7_ATCACG_L008_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	11713549	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76244501132833	34.0	31.0	34.0	31.0	34.0
2	32.89702770697421	34.0	33.0	34.0	31.0	34.0
3	32.90063242148046	34.0	33.0	34.0	31.0	34.0
4	36.2069137201714	37.0	37.0	37.0	35.0	37.0
5	36.19106856512915	37.0	37.0	37.0	35.0	37.0
6	36.221593387281686	37.0	37.0	37.0	35.0	37.0
7	36.21764710251351	37.0	37.0	37.0	35.0	37.0
8	36.21088894578406	37.0	37.0	37.0	35.0	37.0
9	38.002513243424346	39.0	39.0	39.0	37.0	39.0
10-14	38.277985228900306	39.4	39.2	39.4	37.0	39.4
15-19	39.4775744225768	41.0	40.0	41.0	37.0	41.0
20-24	39.3921970873217	41.0	40.0	41.0	37.0	41.0
25-29	39.21505845922529	41.0	39.6	41.0	36.4	41.0
30-34	39.00254506981615	41.0	39.0	41.0	36.0	41.0
35-39	38.754551007555435	40.0	39.0	41.0	35.0	41.0
40-44	38.6752573792964	40.0	38.8	41.0	35.0	41.0
45-49	38.53848293117653	40.0	38.2	41.0	34.8	41.0
50-54	37.90013214611558	39.6	37.8	40.4	33.8	40.8
55-59	38.493179838151526	40.0	38.4	41.0	34.8	41.0
60-64	38.13895305342557	40.0	37.4	41.0	34.0	41.0
65-69	37.57055532870524	39.4	36.4	41.0	33.6	41.0
70-74	36.8102438296028	38.4	35.0	40.4	32.8	41.0
75-79	35.929985967532126	36.8	35.0	39.2	32.0	40.8
80-84	35.00834945924587	35.6	35.0	37.6	31.2	39.2
85-89	34.234208368445806	35.0	34.2	36.4	31.0	38.0
90-94	33.69527155262679	35.0	34.0	35.6	30.2	36.6
95-99	33.30655848197673	35.0	34.0	35.0	29.6	36.0
100-101	32.6735999909165	34.5	33.0	35.0	27.5	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	15.0
4	38.0
5	91.0
6	428.0
7	1927.0
8	4642.0
9	5883.0
10	7095.0
11	8403.0
12	9573.0
13	11314.0
14	13347.0
15	14993.0
16	16518.0
17	18496.0
18	20488.0
19	22418.0
20	24207.0
21	25995.0
22	28235.0
23	30497.0
24	34327.0
25	38308.0
26	44209.0
27	51619.0
28	61325.0
29	71250.0
30	86393.0
31	108435.0
32	141838.0
33	195670.0
34	291549.0
35	482136.0
36	947307.0
37	2261382.0
38	4454362.0
39	2156130.0
40	22706.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.769464769783173	32.81642796677736	30.412073276841113	19.002033986598352
2	20.992143610863092	32.855556077700925	27.879612264340363	18.272688047095613
3	20.651447210427175	31.073666037407587	28.87432694743038	19.400559804734858
4	20.498602318306723	29.75526876821231	29.370054995503985	20.37607391797698
5	20.343197040759613	30.062946655326154	30.0176814527584	19.57617485115583
6	20.53554455176659	30.15569748397498	28.98866237009909	20.320095594159344
7	20.582907431633878	30.122684279578216	29.72075511521759	19.573653173570317
8	19.916460519712388	30.343957659391368	29.88306932516239	19.85651249573386
9	19.97216314328422	29.964364723467863	30.22370275464446	19.83976937860346
10-14	19.997699657270115	30.13148151586812	30.011672710461585	19.85914611640018
15-19	20.047763147524478	29.864712353326382	30.075534186693144	20.011990312456
20-24	20.238699618240656	29.69338638262627	29.872931258846798	20.194982740286278
25-29	20.40820919532795	29.722981339350703	29.628925227320487	20.23988423800086
30-34	20.554120518416582	29.611588846519858	29.5593332692419	20.274957365821656
35-39	20.579360642127973	29.544393143174702	29.50016919953108	20.376077015166246
40-44	20.640630956959438	29.53387894765885	29.45055762199874	20.374932473382973
45-49	20.657134555610128	29.454510763797444	29.395424861995558	20.49292981859687
50-54	20.619656827690395	29.47592938258923	29.42723187814375	20.477181911576633
55-59	20.680616731472416	29.474147228486498	29.36886233471058	20.476373705330506
60-64	20.608076541255013	29.500034558866865	29.35576516618904	20.53612373368908
65-69	20.64256431099023	29.491693510858564	29.303675003758677	20.562067174392524
70-74	20.666488426406996	29.5257517892685	29.29863721692147	20.509122567403033
75-79	20.6775056395202	29.487804528496415	29.312223916150405	20.52246591583299
80-84	20.67346398486946	29.535447349243032	29.296932399177823	20.494156266709687
85-89	20.650556497617767	29.493176451024887	29.288210881927025	20.568056169430324
90-94	20.65704611864613	29.486044512583277	29.26788459510329	20.589024773667305
95-99	20.73750498783193	29.515964495279235	29.17855118004904	20.567979336839798
100-101	20.831118330096192	29.486763730041105	29.09462967900615	20.58748826085655
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	36.771498756381526
2	39.2648316579587
3	40.05200701516203
4	40.874676236283705
5	39.919371891915446
6	40.85564014592593
7	40.15656060520419
8	39.77297301544625
9	39.81193252188768
10-14	39.856845773670294
15-19	40.05975345998047
20-24	40.43368235852694
25-29	40.64809343332881
30-34	40.82907788423824
35-39	40.95543765729422
40-44	41.01556343034241
45-49	41.150064374207
50-54	41.09683873926703
55-59	41.15699043680292
60-64	41.14420027494409
65-69	41.204631485382755
70-74	41.17561099381003
75-79	41.19997155535319
80-84	41.167620251579145
85-89	41.21861266704809
90-94	41.24607089231343
95-99	41.305484324671724
100-101	41.41860659095274
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	258.0
1	276.5
2	328.5
3	423.0
4	520.0
5	613.5
6	813.0
7	1081.0
8	1401.5
9	1715.5
10	2056.5
11	2675.0
12	3540.0
13	4505.5
14	5693.5
15	7347.5
16	9748.0
17	13450.5
18	18962.5
19	27032.5
20	38126.5
21	51996.5
22	68655.0
23	88088.5
24	110527.5
25	136949.0
26	166012.5
27	195695.5
28	227051.0
29	259584.0
30	291479.5
31	321056.5
32	349489.5
33	377116.0
34	401743.5
35	424789.5
36	446350.0
37	463129.0
38	470702.0
39	469459.0
40	461376.5
41	446273.5
42	423060.0
43	395625.0
44	369633.0
45	346744.5
46	328489.5
47	314691.5
48	302049.5
49	288260.5
50	272905.5
51	255090.5
52	235279.5
53	216806.5
54	200683.0
55	185491.5
56	171651.0
57	157491.0
58	141870.0
59	124877.5
60	107545.5
61	90557.0
62	75265.0
63	62315.5
64	51300.0
65	42394.5
66	35146.5
67	28820.0
68	23244.0
69	18682.5
70	15083.0
71	12249.0
72	9812.0
73	7934.5
74	6417.5
75	5333.0
76	4451.5
77	3671.5
78	3136.0
79	2643.0
80	2159.0
81	1687.0
82	1318.0
83	1020.0
84	725.5
85	486.5
86	335.5
87	243.5
88	190.0
89	146.0
90	123.0
91	99.5
92	76.5
93	65.0
94	55.0
95	51.0
96	41.0
97	24.0
98	15.5
99	14.0
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0973829537060032
2	0.08280154887301876
3	0.09302902134955
4	0.09353271156333576
5	0.07849030212790334
6	0.03454973381679626
7	0.013104482680697369
8	0.043308821263307984
9	0.05164105259644195
10-14	0.04890405119746372
15-19	0.020728132865624242
20-24	0.015581955562741915
25-29	0.008248567534911922
30-34	0.009518891328324149
35-39	0.011453403234152176
40-44	0.02545428375294285
45-49	0.023927846291503966
50-54	0.03516782146896726
55-59	0.03890195874879595
60-64	0.026427515691444158
65-69	0.028333001381562497
70-74	0.02998408082810769
75-79	0.044348642755496215
80-84	0.04712320749245169
85-89	0.032248125653463355
90-94	0.0440976513608301
95-99	0.034597541701494566
100-101	0.05511139279820317
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.1713549E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	6.432454247861243
#Duplication Level	Relative count
1	100.0
2	3.570991155060679
3	0.7407170737681784
4	0.4835162494475241
5	0.3461294737234479
6	0.29341129234095353
7	0.21513278059118915
8	0.1682721749178608
9	0.12833415871900145
10++	0.5548721717228196
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCCAG	2087775	3.5921357	3.9144487	25-29
CTGGG	2044450	3.4794521	3.6542368	85-89
GGGGG	1346080	3.2589278	4.1854897	95-97
CCAGG	1862880	3.1755185	3.4638095	25-29
GGAGG	1888705	3.160206	3.433234	70-74
AAAAA	8184745	3.1242514	4.2971163	1
CCTCC	1779760	3.114681	3.5366843	3
CCTGG	1797905	3.0884492	3.256464	3
TTTTT	7751730	3.075098	3.6613379	10-14
>>END_MODULE