##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL7_ATCACG_L007_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	11651783	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.804040205692125	34.0	31.0	34.0	31.0	34.0
2	32.93488902084771	34.0	33.0	34.0	31.0	34.0
3	32.94210783019217	34.0	33.0	34.0	31.0	34.0
4	36.24037119469183	37.0	37.0	37.0	35.0	37.0
5	36.23422518253215	37.0	37.0	37.0	35.0	37.0
6	36.26418857955044	37.0	37.0	37.0	35.0	37.0
7	36.25561710169165	37.0	37.0	37.0	35.0	37.0
8	36.25235845878695	37.0	37.0	37.0	35.0	37.0
9	38.040614985706476	39.0	39.0	39.0	37.0	39.0
10-14	38.32378273780073	39.4	39.2	39.4	37.2	39.4
15-19	39.53966224740025	41.0	40.0	41.0	37.2	41.0
20-24	39.44843039043896	41.0	40.0	41.0	37.0	41.0
25-29	39.29031997935423	41.0	40.0	41.0	36.8	41.0
30-34	39.08078832226793	41.0	39.2	41.0	36.0	41.0
35-39	38.845791343693925	40.2	39.0	41.0	35.4	41.0
40-44	38.79473122697187	40.0	39.0	41.0	35.0	41.0
45-49	38.64865298298123	40.0	38.8	41.0	35.0	41.0
50-54	37.98399197788012	39.6	38.0	40.4	34.4	40.8
55-59	38.567140737173006	40.0	38.6	41.0	35.0	41.0
60-64	38.224186633067234	40.0	37.6	41.0	34.0	41.0
65-69	37.661262503773024	39.6	36.4	41.0	33.8	41.0
70-74	36.918808872427505	38.4	35.2	40.6	33.0	41.0
75-79	36.0306292350278	36.8	35.0	39.2	32.4	41.0
80-84	35.12414256255888	35.8	35.0	37.6	31.8	39.4
85-89	34.346888883872964	35.0	34.6	36.4	31.0	38.0
90-94	33.80082050961643	35.0	34.0	35.8	31.0	36.6
95-99	33.42091580318652	35.0	34.0	35.0	30.0	36.0
100-101	32.82195450258557	34.5	33.5	35.0	28.5	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	5.0
5	26.0
6	227.0
7	1650.0
8	4279.0
9	5231.0
10	6248.0
11	7665.0
12	9141.0
13	10934.0
14	12978.0
15	14354.0
16	15832.0
17	17888.0
18	19803.0
19	21207.0
20	22891.0
21	24237.0
22	26436.0
23	29191.0
24	32352.0
25	36354.0
26	41705.0
27	49430.0
28	59030.0
29	67026.0
30	80781.0
31	101445.0
32	132695.0
33	180899.0
34	272305.0
35	452069.0
36	903813.0
37	2216511.0
38	4488039.0
39	2261640.0
40	25465.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.77124907371494	32.82123840662136	30.421546017429446	18.98596650223425
2	21.01859987031876	32.841985320371855	27.873264542779136	18.266150266530257
3	20.697085937723468	31.023634735792072	28.86669419409725	19.412585132387214
4	20.52761404361227	29.740304831952024	29.365729382140728	20.366351742294974
5	20.341584464077823	30.014790154221043	30.034567036977272	19.609058344723863
6	20.543041914983537	30.126877203438507	28.991105395298028	20.33897548627993
7	20.576067823943198	30.096110871395908	29.72189192833653	19.605929376324365
8	19.918761782828923	30.329211939681528	29.913347389469298	19.83867888802025
9	19.974771308651448	29.95110063277747	30.227749242774436	19.846378815796644
10-14	19.998077146595424	30.12802598324336	30.00195375640572	19.871943113755492
15-19	20.061569854450447	29.864454330091412	30.049947131435488	20.024028684022653
20-24	20.247342002514962	29.695282808350353	29.874481558855393	20.182893630279295
25-29	20.425136747468372	29.70492139831389	29.621273110090424	20.24866874412732
30-34	20.562305210977662	29.615561513704225	29.552352549982082	20.269780725336034
35-39	20.58147644134686	29.54649957920401	29.486824586213846	20.385199393235286
40-44	20.640604752576568	29.525062881318025	29.455147154980367	20.379185211125044
45-49	20.666591801594763	29.44213051550405	29.39090385255992	20.500373830341267
50-54	20.631090701229077	29.47680795642485	29.4198064792898	20.472294863056273
55-59	20.698732981198432	29.46349498246737	29.371471716057247	20.466300320276954
60-64	20.62635255625761	29.493886970638467	29.34803996377268	20.53172050933124
65-69	20.658878658069593	29.482149930720148	29.306024723732165	20.552946687478094
70-74	20.67991630667697	29.50112717248269	29.299972138968496	20.518984381871842
75-79	20.677184594293173	29.490013656552723	29.31729701695546	20.51550473219864
80-84	20.672338045957247	29.527803891616834	29.298983421701646	20.50087464072427
85-89	20.64942664049889	29.483662439806462	29.29098200272352	20.57592891697113
90-94	20.655112456211842	29.459425149369146	29.2840716326344	20.601390761784614
95-99	20.742339835164948	29.502012873986505	29.192572820555302	20.563074470293248
100-101	20.842230128449533	29.47678388272036	29.104936408823512	20.576049580006593
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	36.75721557594919
2	39.28475013684901
3	40.10967107011068
4	40.89396578590725
5	39.95064280880168
6	40.88201740126347
7	40.18199720026756
8	39.75744067084918
9	39.821150124448096
10-14	39.87002026035092
15-19	40.0855985384731
20-24	40.43023563279426
25-29	40.673805491595694
30-34	40.832085936313696
35-39	40.96667583458215
40-44	41.01978996370161
45-49	41.16696563193603
50-54	41.10338556428535
55-59	41.16503330147538
60-64	41.15807306558885
65-69	41.21182534554769
70-74	41.19890068854881
75-79	41.192689326491816
80-84	41.173212686681524
85-89	41.22535555747002
90-94	41.25650321799645
95-99	41.30541430545819
100-101	41.418279708456126
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	274.0
1	279.5
2	317.5
3	436.0
4	512.5
5	594.0
6	816.5
7	1056.5
8	1337.0
9	1647.5
10	2018.5
11	2633.0
12	3467.0
13	4446.5
14	5626.0
15	7256.0
16	9689.5
17	13263.5
18	18709.5
19	26602.0
20	37615.0
21	51687.0
22	68381.5
23	87669.5
24	109799.5
25	135684.5
26	164307.5
27	194123.5
28	225626.5
29	257784.5
30	288851.5
31	318770.5
32	347462.5
33	374831.0
34	399968.0
35	422917.0
36	444670.0
37	461003.5
38	468067.5
39	467267.5
40	459125.0
41	443893.5
42	420116.0
43	392618.0
44	367574.5
45	344809.5
46	325807.0
47	312137.5
48	300331.5
49	286889.0
50	271723.0
51	254482.5
52	235056.0
53	216472.0
54	200207.5
55	185418.0
56	171440.0
57	157361.0
58	142164.0
59	124719.5
60	106993.0
61	90001.0
62	74728.0
63	62019.5
64	51625.0
65	42721.5
66	35073.0
67	28881.5
68	23533.0
69	18812.5
70	15012.0
71	11994.5
72	9623.0
73	7775.0
74	6296.0
75	5185.5
76	4302.0
77	3658.5
78	3074.0
79	2515.0
80	2046.5
81	1627.5
82	1239.5
83	917.5
84	655.0
85	437.5
86	300.0
87	210.0
88	164.5
89	144.5
90	107.5
91	82.0
92	71.0
93	58.0
94	50.0
95	44.5
96	34.5
97	32.0
98	27.5
99	15.0
100	11.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0729416261871681
2	0.08058852452023867
3	0.08635588218558482
4	0.06350959333863324
5	0.05899526278510336
6	0.005793104797780735
7	0.011878010429820054
8	0.011629121482952438
9	0.02015142231879876
10-14	0.02084659489453245
15-19	0.023220480505000824
20-24	0.018436663298655665
25-29	0.0032527210642354053
30-34	0.005041288530690968
35-39	0.008639021169549758
40-44	0.02244806653196339
45-49	0.020432924300083515
50-54	0.022410304071059338
55-59	0.025659592184303467
60-64	0.021047422527522182
65-69	0.024008342757499003
70-74	0.027937355167016068
75-79	0.029655547138150447
80-84	0.02574713243458104
85-89	0.020963315228235885
90-94	0.0245267183571819
95-99	0.037396851623481144
100-101	0.033145141820784
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.1651783E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	6.328325174720593
#Duplication Level	Relative count
1	100.0
2	3.502722033303
3	0.7242790469956522
4	0.5044941488257739
5	0.35708393867309524
6	0.26555124500428395
7	0.21286672946235186
8	0.1750828849827844
9	0.13942770779784047
10++	0.5518569961311471
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCCAG	2078660	3.5986574	3.9245648	25-29
CTGGG	2031760	3.4742355	3.6725712	85-89
GGGGG	1334520	3.2467117	4.2133203	95-97
CCAGG	1853500	3.1781878	3.422297	25-29
GGAGG	1882925	3.1672304	3.424953	2
AAAAA	8211110	3.1561718	4.2730627	1
CCTCC	1771385	3.1175272	3.4637275	3
CCTGG	1793725	3.0967977	3.3291495	3
TTTTT	7725265	3.072716	3.640249	10-14
>>END_MODULE