##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL7_ATCACG_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	11651783	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.22014476239388	34.0	33.0	34.0	31.0	34.0
2	33.36626814969005	34.0	34.0	34.0	31.0	34.0
3	33.39459497314703	34.0	34.0	34.0	31.0	34.0
4	36.640269133058865	37.0	37.0	37.0	35.0	37.0
5	36.583116506718326	37.0	37.0	37.0	35.0	37.0
6	36.573851229464196	37.0	37.0	37.0	35.0	37.0
7	36.575851610006815	37.0	37.0	37.0	35.0	37.0
8	36.5731521948186	37.0	37.0	37.0	35.0	37.0
9	38.43535517268044	39.0	39.0	39.0	37.0	39.0
10-14	38.73777817523721	39.4	39.2	39.4	37.2	39.4
15-19	40.01715119479997	41.0	40.0	41.0	38.0	41.0
20-24	39.9604584980685	41.0	40.0	41.0	38.0	41.0
25-29	39.801534305951286	41.0	40.0	41.0	38.0	41.0
30-34	39.64484060508164	41.0	40.0	41.0	37.6	41.0
35-39	39.44836521586439	41.0	40.0	41.0	37.0	41.0
40-44	39.2596428889896	41.0	39.0	41.0	36.2	41.0
45-49	39.11792372034391	40.2	39.0	41.0	35.8	41.0
50-54	39.084326922325964	40.6	39.0	41.0	35.4	41.0
55-59	38.78718623578898	40.0	38.4	41.0	35.0	41.0
60-64	38.32052297918697	40.0	37.4	41.0	34.6	41.0
65-69	37.661917939941034	39.2	36.2	41.0	34.0	41.0
70-74	37.179663266986694	38.4	35.2	40.4	34.0	41.0
75-79	36.04127097114665	36.4	34.8	39.2	32.6	40.2
80-84	35.65084885291805	35.8	35.0	37.8	33.0	39.4
85-89	34.89233354242866	35.0	35.0	36.6	33.0	38.0
90-94	34.34235527729962	35.0	35.0	35.8	32.4	36.8
95-99	34.01528150670159	35.0	34.8	35.0	32.0	36.0
100-101	33.67885142557152	35.0	34.0	35.0	31.5	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	3.0
11	10.0
12	16.0
13	92.0
14	341.0
15	1086.0
16	2669.0
17	5062.0
18	7422.0
19	9273.0
20	10827.0
21	12669.0
22	14517.0
23	17464.0
24	21241.0
25	26088.0
26	31328.0
27	38156.0
28	47150.0
29	59376.0
30	73593.0
31	91122.0
32	118080.0
33	160750.0
34	244393.0
35	419188.0
36	868739.0
37	2230647.0
38	4659915.0
39	2447306.0
40	33255.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.09137488006124	30.925223442255174	30.192099404554497	19.791302273129084
2	20.029353155940637	30.29743867578281	30.46782568630319	19.205382481973363
3	20.335725302085827	29.45668751378579	30.29284666596289	19.914740518165488
4	20.291547021188062	29.231055503142713	30.388715628961005	20.088681846708223
5	20.576095521174743	29.563595545849076	30.2745768608976	19.58573207207858
6	20.560338276124778	29.725888303961717	29.565646734066362	20.14812668584714
7	20.539071144733814	29.97042598544789	29.873702591268646	19.616800278549643
8	20.11922123850058	29.965370965113237	29.90751715853273	20.00789063785345
9	20.070825211901045	29.71121243847401	30.17608549695785	20.041876852667098
10-14	20.04504892644692	29.852350405637733	30.06593157987246	20.03666908804289
15-19	20.03937605939022	29.678898019610788	30.142093676915483	20.139632244083508
20-24	20.157064710684146	29.568751527267388	30.007808074078657	20.266375687969806
25-29	20.329255705692827	29.556367443260314	29.77520048522826	20.339176365818602
30-34	20.43648405402334	29.53532943711782	29.696768439075083	20.331418069783755
35-39	20.553823875233853	29.43564582194396	29.59449835031551	20.416031952506668
40-44	20.556103188038293	29.42679138059322	29.56983969242295	20.447265738945536
45-49	20.60179038403856	29.360093689633686	29.532537351461635	20.505578574866117
50-54	20.527817079179002	29.400360438752156	29.53724749821459	20.534574983854256
55-59	20.619755070590244	29.339377005163282	29.50732806083	20.533539863416472
60-64	20.57171586805381	29.358723387264014	29.494731228916223	20.574829515765956
65-69	20.596579080162943	29.390943316597966	29.409541603009416	20.602936000229676
70-74	20.630542450370466	29.415643938623354	29.38417089350195	20.569642717504227
75-79	20.616292166775075	29.374529946623362	29.42545468479436	20.583723201807203
80-84	20.630067964600933	29.3877349731593	29.423450706271886	20.558746355967884
85-89	20.589247337523037	29.39778816631279	29.38450608119332	20.62845841497086
90-94	20.592210359383426	29.35090883283597	29.378174126928613	20.67870668085199
95-99	20.61494675341962	29.40830327429536	29.313895527380634	20.662854444904386
100-101	20.72597447800354	29.35874538814744	29.20821716401959	20.70706296982943
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	38.88267715319033
2	39.234735637914
3	40.250465820251314
4	40.380228867896285
5	40.16182759325332
6	40.70846496197191
7	40.15587142328346
8	40.127111876354036
9	40.11270206456815
10-14	40.08171801448981
15-19	40.17900830347373
20-24	40.42344039865395
25-29	40.66843207151143
30-34	40.76790212380709
35-39	40.969855827740524
40-44	41.00336892698383
45-49	41.10736895890468
50-54	41.06239206303326
55-59	41.153294934006716
60-64	41.14654538381976
65-69	41.199515080392615
70-74	41.2001851678747
75-79	41.20001536858228
80-84	41.18881432056881
85-89	41.2177057524939
90-94	41.27091704023542
95-99	41.277801198324006
100-101	41.433037447832966
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	179.0
1	165.0
2	182.0
3	253.0
4	341.0
5	405.5
6	535.5
7	774.0
8	1037.5
9	1316.5
10	1623.0
11	2159.5
12	3007.0
13	3950.0
14	5053.5
15	6690.5
16	8945.5
17	12566.5
18	18160.0
19	25855.5
20	36837.0
21	50577.0
22	67247.5
23	86873.5
24	109257.5
25	135047.5
26	163544.0
27	193669.0
28	224830.0
29	256690.5
30	288659.0
31	318569.5
32	346626.0
33	373963.0
34	399921.5
35	424006.0
36	445706.0
37	461186.0
38	468310.0
39	468356.0
40	460451.5
41	444693.5
42	421433.0
43	394453.5
44	368291.0
45	345511.5
46	326888.0
47	312944.0
48	301281.0
49	288558.5
50	273191.5
51	254995.0
52	235328.5
53	216467.5
54	200414.0
55	185811.5
56	171962.5
57	158240.5
58	142675.0
59	125508.5
60	107786.5
61	90523.0
62	74995.5
63	62126.5
64	51738.0
65	42978.0
66	35381.5
67	28608.0
68	22936.5
69	18467.5
70	14890.5
71	11785.0
72	9440.5
73	7603.5
74	6088.5
75	4989.5
76	4183.5
77	3511.5
78	2871.5
79	2317.0
80	1811.0
81	1369.0
82	1018.5
83	766.0
84	513.0
85	299.0
86	199.0
87	137.5
88	95.5
89	66.5
90	51.0
91	37.5
92	25.0
93	22.0
94	19.0
95	15.0
96	10.5
97	6.0
98	5.0
99	5.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.5747132434581045E-5
2	0.01070222471530752
3	0.0025146366011107486
4	0.005964752347344608
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	6.007664234735577E-5
15-19	2.8321845678039146E-4
20-24	0.0021816403549568338
25-29	0.011574194267091997
30-34	0.007028967154640625
35-39	0.029995409286286915
40-44	0.02447179114132146
45-49	0.02668261157970415
50-54	0.027480772685176166
55-59	0.023979162674073143
60-64	0.05388016580809993
65-69	0.09376762337575288
70-74	0.015568432745443337
75-79	0.017526931285967134
80-84	0.0028768129306905215
85-89	0.012906179251707657
90-94	0.015515222005078535
95-99	0.00896171856272984
100-101	0.0012659006780335678
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.1651783E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	6.3146714007170965
#Duplication Level	Relative count
1	100.0
2	3.4497258742747645
3	0.70793633895779
4	0.5248310001596849
5	0.39069569383084046
6	0.29754617554692075
7	0.22142971203491776
8	0.1676691329110555
9	0.12721562782775322
10++	0.5695427689359663
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCCAG	2086885	3.5965111	3.9333258	25-29
CTGGG	2038985	3.4836276	3.681101	65-69
CCAGG	1867330	3.2113886	3.4641814	25-29
GGAGG	1834085	3.1410067	3.4236515	70-74
CCTCC	1810145	3.1121788	3.4183154	65-69
CCTGG	1792805	3.0694597	3.2989151	3
>>END_MODULE