##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename ZL6_CTTGTA_L008_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15696073 Filtered Sequences 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.747683258099016 34.0 31.0 34.0 31.0 34.0 2 32.882149184703714 34.0 33.0 34.0 31.0 34.0 3 32.887889155459455 34.0 33.0 34.0 31.0 34.0 4 36.19415881921548 37.0 37.0 37.0 35.0 37.0 5 36.1788655672027 37.0 37.0 37.0 35.0 37.0 6 36.20365265885295 37.0 37.0 37.0 35.0 37.0 7 36.205737065570474 37.0 37.0 37.0 35.0 37.0 8 36.192250953470975 37.0 37.0 37.0 35.0 37.0 9 37.98472541507675 39.0 39.0 39.0 37.0 39.0 10-14 38.26176423873666 39.4 39.2 39.4 37.0 39.4 15-19 39.46143184986462 41.0 40.0 41.0 37.0 41.0 20-24 39.37364714091226 41.0 40.0 41.0 37.0 41.0 25-29 39.20069042747189 41.0 39.6 41.0 36.2 41.0 30-34 38.992313325759895 41.0 39.0 41.0 36.0 41.0 35-39 38.74406679938352 40.0 39.0 41.0 35.0 41.0 40-44 38.65862388636954 40.0 38.8 41.0 35.0 41.0 45-49 38.5234518086148 40.0 38.2 41.0 34.8 41.0 50-54 37.88480402709646 39.6 37.8 40.4 33.8 40.8 55-59 38.47281097635058 40.0 38.4 41.0 34.6 41.0 60-64 38.112507440555355 40.0 37.4 41.0 34.0 41.0 65-69 37.5432232508093 39.4 36.2 41.0 33.4 41.0 70-74 36.78508006429379 38.4 35.0 40.4 32.8 41.0 75-79 35.902912722182165 36.8 35.0 39.2 32.0 40.8 80-84 34.98154602109712 35.6 35.0 37.6 31.0 39.2 85-89 34.20821158260413 35.0 34.2 36.4 31.0 37.8 90-94 33.669786232518156 35.0 34.0 35.6 30.0 36.6 95-99 33.28209017631353 35.0 34.0 35.0 29.6 36.0 100-101 32.651454411558866 34.5 33.0 35.0 27.5 35.5 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 17.0 4 77.0 5 140.0 6 754.0 7 4222.0 8 9767.0 9 8838.0 10 8333.0 11 9383.0 12 11342.0 13 14329.0 14 17274.0 15 19708.0 16 21797.0 17 25012.0 18 27965.0 19 30416.0 20 32885.0 21 35477.0 22 38694.0 23 42413.0 24 46845.0 25 52914.0 26 61378.0 27 70914.0 28 83120.0 29 97454.0 30 117330.0 31 146984.0 32 191640.0 33 264181.0 34 393762.0 35 650428.0 36 1274884.0 37 3033222.0 38 5958445.0 39 2864091.0 40 29638.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 18.024743793325555 32.71004483218224 29.469921623396917 19.795289751095297 2 20.83196613488226 32.73457586539039 28.20816809520573 18.22528990452162 3 20.593072047138648 30.81453186577729 28.972942466871583 19.619453620212482 4 20.404962908659034 29.77194857973401 29.372121728776364 20.450966782830594 5 20.318336770552623 30.03313949095574 29.992689865054206 19.65583387343743 6 20.48625001744683 30.07446084195122 29.085399223952237 20.35388991664972 7 20.595332808546054 30.115978243374624 29.647161478116 19.641527469963318 8 19.91383217064022 30.22545517124892 29.93607046908214 19.924642189028724 9 19.931962689883257 29.916904552264384 30.233729093631172 19.91740366422119 10-14 20.021702338322545 30.09669891187574 29.940306841100078 19.941291908701636 15-19 20.08084116656631 29.865733047661713 29.99634711385545 20.057078671916532 20-24 20.266969379624285 29.688483260888887 29.810439710111776 20.234107649375048 25-29 20.44076837296287 29.68269183105153 29.571867680286445 20.304672115699148 30-34 20.558289365076224 29.601611444263725 29.505329566095913 20.334769624564135 35-39 20.598169752866553 29.528811590775085 29.44765842625151 20.425360230106854 40-44 20.63697616190557 29.529842704390934 29.40816754407151 20.425013589631988 45-49 20.655551108657146 29.447441845431854 29.37523079738366 20.52177624852734 50-54 20.636080514706638 29.47846105877761 29.384550492661777 20.500907933853973 55-59 20.706165789238867 29.455655266478857 29.334093487488687 20.504085456793586 60-64 20.647488814380974 29.456186102842747 29.331697945410735 20.564627137365548 65-69 20.67227583519237 29.44147771300218 29.295220237748982 20.59102621405647 70-74 20.69140714663618 29.48088720306891 29.266959380441616 20.560746269853297 75-79 20.69306358682145 29.450080367332916 29.295083493607375 20.561772552238256 80-84 20.697702811387515 29.492668396309224 29.281728697154946 20.527900095148315 85-89 20.653668053138084 29.454319632332272 29.27758110847592 20.614431206053723 90-94 20.657580722698903 29.43262734563637 29.26302263897637 20.646769292688358 95-99 20.702488159261403 29.464393809784458 29.209491649553755 20.623626381400385 100-101 20.80064768266227 29.42052468899707 29.1218119458579 20.65701568248276 >>END_MODULE >>Per base GC content pass #Base %GC 1 37.82003354442085 2 39.05725603940388 3 40.21252566735113 4 40.85592969148963 5 39.97417064399006 6 40.84013993409655 7 40.236860278509376 8 39.83847435966894 9 39.84936635410444 10-14 39.96299424702418 15-19 40.13791983848284 20-24 40.50107702899934 25-29 40.74544048866202 30-34 40.893058989640366 35-39 41.02352998297341 40-44 41.06198975153756 45-49 41.17732735718448 50-54 41.13698844856061 55-59 41.210251246032456 60-64 41.212115951746526 65-69 41.26330204924884 70-74 41.25215341648947 75-79 41.254836139059705 80-84 41.225602906535826 85-89 41.26809925919181 90-94 41.30435001538726 95-99 41.326114540661784 100-101 41.45766336514503 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 364.0 1 384.5 2 440.5 3 545.5 4 684.5 5 795.5 6 1006.0 7 1345.5 8 1816.5 9 2273.0 10 2747.0 11 3526.0 12 4697.0 13 6016.0 14 7680.0 15 10038.0 16 13009.5 17 17291.5 18 24187.5 19 34364.5 20 48058.0 21 65977.0 22 88256.0 23 114519.5 24 145468.5 25 180709.5 26 219648.5 27 260828.5 28 303458.5 29 346993.5 30 388760.0 31 429180.0 32 468622.0 33 506249.5 34 541861.5 35 574283.5 36 603389.5 37 625895.5 38 636512.5 39 633391.5 40 618020.0 41 593315.5 42 559738.5 43 521555.0 44 487367.5 45 459809.0 46 437964.5 47 421062.5 48 405488.0 49 387756.0 50 367653.5 51 344301.5 52 317958.5 53 292751.0 54 270580.5 55 250331.5 56 231294.0 57 212752.5 58 191754.0 59 168631.0 60 145784.0 61 122817.0 62 101895.5 63 84852.5 64 70528.0 65 58400.5 66 48090.0 67 39508.5 68 32249.5 69 25831.0 70 20746.5 71 16703.0 72 13347.5 73 10812.0 74 8968.0 75 7508.5 76 6281.5 77 5402.0 78 4566.5 79 3769.0 80 3051.5 81 2430.5 82 1927.5 83 1466.5 84 1093.0 85 761.5 86 510.0 87 351.5 88 268.0 89 223.5 90 182.5 91 145.5 92 110.0 93 78.5 94 53.5 95 50.0 96 39.0 97 27.0 98 29.0 99 19.5 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09794169535271657 2 0.08378528820552758 3 0.09348198112992977 4 0.09419553540557565 5 0.07879677929632463 6 0.035320936644471515 7 0.013347287566769091 8 0.04410657366336153 9 0.05230607681297099 10-14 0.04975512027753694 15-19 0.020872736766705913 20-24 0.015532547535934626 25-29 0.008275955393428663 30-34 0.009752757903202923 35-39 0.011461465552562097 40-44 0.025248353521291598 45-49 0.023811051337490594 50-54 0.03575926284236828 55-59 0.03996541045648807 60-64 0.026367104689179265 65-69 0.028227442622113186 70-74 0.029385694115974102 75-79 0.045306873891323006 80-84 0.04786929826332994 85-89 0.03319174165410673 90-94 0.04537185829856933 95-99 0.035564309620629314 100-101 0.05581650900833604 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5696073E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 6.6755993444942 #Duplication Level Relative count 1 100.0 2 3.4644719495727454 3 0.6917201735705251 4 0.49744074210472944 5 0.3794853730004964 6 0.29248661660235165 7 0.23751194231395342 8 0.2118926766261562 9 0.152114390021296 10++ 0.8198165020095112 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position CCCAG 2810570 3.5852215 3.863229 25-29 CTGGG 2752970 3.489988 3.652114 85-89 GGAGG 2545575 3.1809113 3.4430685 70-74 CCAGG 2509890 3.1797352 3.4027452 25-29 AAAAA 10994820 3.1470718 4.253892 1 GGGGG 1732940 3.1301198 4.172589 95-97 CCTCC 2414420 3.1245768 3.4906585 3 CCTGG 2429340 3.1009595 3.440521 3 TTTTT 10383640 3.086211 3.6263144 10-14 >>END_MODULE