##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename ZL6_CTTGTA_L008_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15696073 Filtered Sequences 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.234063704978944 34.0 33.0 34.0 31.0 34.0 2 33.36696070412007 34.0 34.0 34.0 31.0 34.0 3 33.387061782905825 34.0 34.0 34.0 31.0 34.0 4 36.6297617881874 37.0 37.0 37.0 35.0 37.0 5 36.57078735553791 37.0 37.0 37.0 35.0 37.0 6 36.55466319505522 37.0 37.0 37.0 35.0 37.0 7 36.557983006322665 37.0 37.0 37.0 35.0 37.0 8 36.56323189883228 37.0 37.0 37.0 35.0 37.0 9 38.41103612349407 39.0 39.0 39.0 37.0 39.0 10-14 38.71620690092356 39.4 39.2 39.4 37.2 39.4 15-19 39.984374142500485 41.0 40.0 41.0 38.0 41.0 20-24 39.91828215885592 41.0 40.0 41.0 38.0 41.0 25-29 39.74977999911188 41.0 40.0 41.0 38.0 41.0 30-34 39.581515351005315 41.0 40.0 41.0 37.2 41.0 35-39 39.37365549969091 41.0 39.6 41.0 36.8 41.0 40-44 39.17129600505808 40.6 39.0 41.0 36.0 41.0 45-49 39.02396678455815 40.0 39.0 41.0 35.4 41.0 50-54 38.992416791129855 40.0 39.0 41.0 35.0 41.0 55-59 38.677535584856166 40.0 38.2 41.0 35.0 41.0 60-64 38.207325399161945 40.0 37.4 41.0 34.2 41.0 65-69 37.549293979455875 39.0 36.0 41.0 33.6 41.0 70-74 37.07805175218031 38.4 35.2 40.4 33.6 41.0 75-79 35.94239447026017 36.4 34.8 39.2 32.4 40.2 80-84 35.557958834671574 35.8 35.0 37.8 33.0 39.4 85-89 34.78142931674694 35.0 35.0 36.6 32.6 38.0 90-94 34.23374199393695 35.0 35.0 35.8 32.0 36.8 95-99 33.90131360882432 35.0 34.0 35.0 32.0 36.0 100-101 33.54825398047015 35.0 34.0 35.0 30.5 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 2.0 11 15.0 12 20.0 13 97.0 14 396.0 15 1222.0 16 3139.0 17 6434.0 18 9587.0 19 12372.0 20 14939.0 21 17669.0 22 21079.0 23 25199.0 24 30544.0 25 37121.0 26 46122.0 27 55606.0 28 67964.0 29 83849.0 30 105446.0 31 132134.0 32 172531.0 33 235708.0 34 358951.0 35 612547.0 36 1247093.0 37 3082870.0 38 6169820.0 39 3105773.0 40 39823.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.055916131950255 31.038170128474018 29.66363469033531 20.24227904924042 2 20.201716887226816 30.26758707164231 30.506410691377305 19.024285349753573 3 20.256538052543462 29.34965325403367 30.313091688602622 20.08071700482025 4 20.21982823346961 29.273328430620833 30.35923698876783 20.147606347141732 5 20.50274613274288 29.585852461313095 30.249929393167324 19.6614720127767 6 20.492826454107345 29.70420053474522 29.617796757188884 20.185176253958552 7 20.555154145880948 29.940393371004326 29.84277659768784 19.66167588542688 8 20.037999313586273 29.85908003868229 29.999771280370574 20.103149367360867 9 20.050327237902117 29.660285091691406 30.232020455052677 20.057367215353803 10-14 20.075105840774672 29.81515971315718 30.034985384477036 20.07474906159111 15-19 20.086360988130174 29.655763115569528 30.09053189067396 20.16734400562634 20-24 20.214567324766115 29.548630253908186 29.924780947009122 20.312021474316577 25-29 20.375748653286198 29.511735595133022 29.7315854937604 20.38093025782038 30-34 20.4933680666222 29.48588669276952 29.653060492927917 20.367684747680364 35-39 20.56267571658949 29.420056851815634 29.575320345429184 20.441947086165687 40-44 20.58404120166318 29.420695790634984 29.54179304168584 20.453469966015998 45-49 20.61506129179378 29.35288398108088 29.49875771364663 20.533297013478713 50-54 20.55566348760164 29.382398472513227 29.52847061505755 20.53346742482758 55-59 20.62472504566764 29.336570585855 29.48679239790867 20.5519119705687 60-64 20.583449270781145 29.35778754650866 29.467001572002292 20.591761610707895 65-69 20.622218357938237 29.337087849660332 29.425069740428555 20.615624051972876 70-74 20.664830534036835 29.375239776596374 29.37619827754575 20.58373141182104 75-79 20.663303682281832 29.33339934917051 29.408738967517404 20.594558001030254 80-84 20.654595348844083 29.34318995335602 29.405285594201253 20.596929103598644 85-89 20.62519116735453 29.33850870091842 29.395793910376668 20.640506221350385 90-94 20.609924523404494 29.30617293813903 29.390448937595227 20.69345360086125 95-99 20.62694877844744 29.34577091986491 29.338770677080227 20.688509624607423 100-101 20.729085181733886 29.287782557731628 29.23678751082625 20.746344749708232 >>END_MODULE >>Per base GC content pass #Base %GC 1 39.29819518119068 2 39.22600223698039 3 40.33725505736371 4 40.36743458061134 5 40.164218145519584 6 40.6780027080659 7 40.21683003130783 8 40.14114868094714 9 40.107694453255924 10-14 40.14985490236578 15-19 40.253704993756514 20-24 40.52658879908269 25-29 40.75667891110658 30-34 40.86105281430257 35-39 41.00462280275517 40-44 41.03751116767918 45-49 41.148358305272495 50-54 41.08913091242922 55-59 41.176637016236334 60-64 41.175210881489036 65-69 41.23784240991111 70-74 41.24856194585787 75-79 41.257861683312086 80-84 41.25152445244273 85-89 41.265697388704915 90-94 41.30337812426574 95-99 41.31545840305486 100-101 41.475429931442115 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 223.0 1 207.5 2 232.0 3 333.5 4 433.5 5 518.5 6 721.5 7 1034.5 8 1407.5 9 1771.0 10 2183.0 11 2915.5 12 4033.5 13 5460.5 14 6887.5 15 8992.5 16 12062.5 17 16289.0 18 23172.5 19 33171.0 20 46890.5 21 64728.0 22 86706.5 23 113276.0 24 144212.5 25 179883.5 26 218386.0 27 259705.0 28 303646.5 29 346430.0 30 388054.0 31 429320.0 32 468963.0 33 506660.5 34 542392.0 35 575397.0 36 604272.5 37 625541.5 38 636286.0 39 633765.5 40 619631.0 41 595603.0 42 562393.5 43 524137.0 44 488828.5 45 460806.5 46 437627.0 47 420297.0 48 405626.5 49 389089.0 50 368976.5 51 345573.0 52 319382.0 53 293323.0 54 271030.5 55 250851.0 56 232268.5 57 213686.0 58 193200.0 59 170246.0 60 146415.0 61 123623.5 62 102566.0 63 84872.5 64 70461.5 65 58301.5 66 47826.0 67 39244.0 68 32124.5 69 25772.5 70 20469.5 71 16224.0 72 13027.5 73 10657.0 74 8663.5 75 7100.5 76 5951.0 77 5060.0 78 4302.5 79 3604.5 80 2939.5 81 2259.5 82 1690.5 83 1232.5 84 853.0 85 586.5 86 365.5 87 221.5 88 166.5 89 115.5 90 87.0 91 69.5 92 50.0 93 36.5 94 30.0 95 23.5 96 16.0 97 12.0 98 10.0 99 6.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0023572775177587413 2 0.00514778441715963 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 5.733918286440181E-4 15-19 0.0012079454523433982 20-24 4.867459523155887E-4 25-29 0.01333199711800525 30-34 0.008848073018009026 35-39 0.0298367623545074 40-44 0.016377344830136812 45-49 0.01855113696272947 50-54 0.026126280121148773 55-59 0.019192061606747114 60-64 0.03916138769232278 65-69 0.08183957860032888 70-74 0.03124603204890803 75-79 0.018278457293107647 80-84 0.005921226283797228 85-89 0.01922136830021114 90-94 0.03192008599858066 95-99 0.014854670974071029 100-101 0.00274590975717302 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5696073E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 6.529082734979621 #Duplication Level Relative count 1 100.0 2 3.3759328656993692 3 0.6913082006768948 4 0.5210168369580304 5 0.3603343903142116 6 0.31175597621259193 7 0.24823035777201244 8 0.19271217022730427 9 0.17242667862443015 10++ 0.8920278018000705 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position CCCAG 2822985 3.5962234 3.9531746 25-29 CTGGG 2764465 3.4903693 3.6742287 1 CCAGG 2517000 3.2005134 3.4158201 25-29 GGAGG 2487405 3.151227 3.424814 70-74 CCTCC 2460830 3.1242576 3.408771 70-74 CCTGG 2444605 3.0922217 3.2925487 3 >>END_MODULE