##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL6_CTTGTA_L007_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	15628660	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78932051756197	34.0	31.0	34.0	31.0	34.0
2	32.91981385480265	34.0	33.0	34.0	31.0	34.0
3	32.929229825205745	34.0	33.0	34.0	31.0	34.0
4	36.226369119297495	37.0	37.0	37.0	35.0	37.0
5	36.220262005827756	37.0	37.0	37.0	35.0	37.0
6	36.24476621796111	37.0	37.0	37.0	35.0	37.0
7	36.24233741088487	37.0	37.0	37.0	35.0	37.0
8	36.23343517614434	37.0	37.0	37.0	35.0	37.0
9	38.02051225121028	39.0	39.0	39.0	37.0	39.0
10-14	38.30529769026903	39.4	39.2	39.4	37.2	39.4
15-19	39.52005379859822	41.0	40.0	41.0	37.0	41.0
20-24	39.42857112509965	41.0	40.0	41.0	37.0	41.0
25-29	39.273332301041805	41.0	40.0	41.0	36.6	41.0
30-34	39.06702130572935	41.0	39.2	41.0	36.0	41.0
35-39	38.832418377519254	40.2	39.0	41.0	35.2	41.0
40-44	38.77577318848832	40.0	39.0	41.0	35.0	41.0
45-49	38.62995684850781	40.0	38.6	41.0	35.0	41.0
50-54	37.965761082524025	39.6	38.0	40.4	34.4	40.8
55-59	38.545511707337674	40.0	38.6	41.0	35.0	41.0
60-64	38.19709918828614	40.0	37.4	41.0	34.0	41.0
65-69	37.63326500160603	39.4	36.4	41.0	33.8	41.0
70-74	36.89138896104976	38.4	35.2	40.6	33.0	41.0
75-79	36.00258764347039	36.8	35.0	39.2	32.2	41.0
80-84	35.09464796086165	35.6	35.0	37.6	31.8	39.2
85-89	34.31675052115792	35.0	34.6	36.4	31.0	37.8
90-94	33.77325101448237	35.0	34.0	35.6	30.8	36.6
95-99	33.393735854513444	35.0	34.0	35.0	30.0	36.0
100-101	32.798327335804856	34.5	33.5	35.0	28.5	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	3.0
4	11.0
5	39.0
6	515.0
7	4041.0
8	9362.0
9	8313.0
10	7592.0
11	8832.0
12	10708.0
13	13801.0
14	16847.0
15	19132.0
16	21242.0
17	24107.0
18	27123.0
19	29107.0
20	31616.0
21	33642.0
22	36357.0
23	39734.0
24	44247.0
25	49334.0
26	57168.0
27	67826.0
28	80442.0
29	90783.0
30	110232.0
31	137742.0
32	178885.0
33	245002.0
34	368750.0
35	610137.0
36	1217901.0
37	2975631.0
38	6015583.0
39	3003948.0
40	32924.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.03044295269696	32.715845583004345	29.464306639443137	19.789404824855563
2	20.83509677492069	32.71557324963334	28.21759489172837	18.23173508371761
3	20.60760470996053	30.809756857314362	28.94830764332071	19.6343307894044
4	20.426529806422053	29.76085418904536	29.35593598422585	20.456680020306734
5	20.309652032809737	30.008870523962738	29.974195130399263	19.707282312828266
6	20.478730807232825	30.09820320934044	29.05446656663487	20.368599416791866
7	20.610940502761963	30.098045282454283	29.648073538473334	19.642940676310424
8	19.92622644125898	30.203663119178366	29.947365211304188	19.922745228258464
9	19.961615060197822	29.899799678715404	30.212764486846066	19.92582077424071
10-14	20.023507232678124	30.09317198818589	29.952347979643758	19.93097279949223
15-19	20.095555324442596	29.869457515660123	29.96969767758142	20.06528948231586
20-24	20.273667558294118	29.686695073958862	29.800738262613585	20.238899105133434
25-29	20.43709237937807	29.674949562537904	29.576206046552333	20.31175201153169
30-34	20.574335294866085	29.604604263205342	29.493777735266768	20.327282706661805
35-39	20.598698458908267	29.524883032592175	29.447285292356323	20.42913321614324
40-44	20.630979952078977	29.527258346627377	29.40896106158207	20.432800639711576
45-49	20.665001223521397	29.44307671331971	29.37288582887796	20.51903623428094
50-54	20.638834954612797	29.483090123387086	29.377964311418825	20.500110610581288
55-59	20.716144274287316	29.454096910687692	29.324202516620844	20.505556298404148
60-64	20.644874451481616	29.463789242991833	29.319269537289394	20.57206676823715
65-69	20.663803511657854	29.437581032402132	29.296416698201	20.60219875773901
70-74	20.708145286368566	29.470650298319484	29.26141146804006	20.559792947271887
75-79	20.694651010058116	29.4471106227426	29.289452193859628	20.568786173339657
80-84	20.703230138357696	29.48407248333883	29.276414011557872	20.5362833667456
85-89	20.666340417968076	29.45454623183969	29.26869762348094	20.610415726711295
90-94	20.65402269702841	29.431057206912037	29.26147468923579	20.653445406823767
95-99	20.71348722497387	29.460506853238755	29.200856731975584	20.62514918981179
100-101	20.79013916926518	29.42156640039213	29.13276148410946	20.655532946233233
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	37.819847777552525
2	39.066831858638295
3	40.24193549936493
4	40.88320982672879
5	40.016934345638
6	40.84733022402469
7	40.25388117907239
8	39.84897166951744
9	39.887435834438534
10-14	39.95448003217035
15-19	40.160844806758455
20-24	40.51256666342755
25-29	40.74884439090976
30-34	40.901618001527886
35-39	41.0278316750515
40-44	41.063780591790554
45-49	41.18403745780233
50-54	41.138945565194085
55-59	41.22170057269147
60-64	41.21694121971877
65-69	41.266002269396864
70-74	41.26793823364046
75-79	41.26343718339777
80-84	41.2395135051033
85-89	41.276756144679375
90-94	41.30746810385218
95-99	41.33863641478566
100-101	41.44567211549841
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	401.0
1	388.0
2	425.5
3	557.5
4	692.0
5	800.5
6	1039.5
7	1365.5
8	1773.0
9	2246.5
10	2751.5
11	3582.0
12	4677.0
13	5979.5
14	7562.0
15	9693.5
16	12666.0
17	17165.5
18	23895.0
19	33770.5
20	47625.0
21	65692.5
22	87773.5
23	114103.0
24	144812.0
25	179976.0
26	218712.5
27	259400.5
28	302143.0
29	345599.0
30	387539.5
31	426907.0
32	465195.5
33	503275.5
34	538694.0
35	570832.5
36	600501.5
37	622531.0
38	632934.0
39	629934.5
40	615301.0
41	591007.5
42	557060.5
43	519951.5
44	485075.5
45	457149.0
46	435675.5
47	418700.5
48	403710.5
49	386754.0
50	366449.5
51	343027.0
52	317597.0
53	292463.5
54	270152.0
55	249935.5
56	231327.0
57	212560.0
58	191569.5
59	168718.5
60	145525.5
61	122778.0
62	102383.0
63	85089.0
64	70408.0
65	58315.5
66	48315.5
67	39622.5
68	31980.5
69	25776.5
70	20700.5
71	16468.0
72	13168.5
73	10743.0
74	8874.0
75	7391.0
76	6077.0
77	5120.5
78	4438.0
79	3669.0
80	2954.0
81	2384.0
82	1879.5
83	1376.0
84	960.5
85	669.5
86	473.5
87	334.0
88	239.0
89	191.0
90	160.0
91	129.0
92	98.5
93	82.5
94	74.0
95	57.5
96	44.5
97	39.0
98	31.5
99	24.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07536154731115784
2	0.08247028216110658
3	0.08853606131299804
4	0.06524551689012366
5	0.06088173906144225
6	0.006161756670117591
7	0.012163550809858299
8	0.012304317836589956
9	0.02086551246236082
10-14	0.021379951960052875
15-19	0.024218327099060313
20-24	0.019200622446198204
25-29	0.0031646987009762836
30-34	0.0052032611880993
35-39	0.008963020502077593
40-44	0.023152336796628755
45-49	0.020917980172324435
50-54	0.022859285440978306
55-59	0.02636950320756866
60-64	0.02166532511424524
65-69	0.024901687028830367
70-74	0.02882268857342856
75-79	0.030437670280113585
80-84	0.02661136655349851
85-89	0.021507921984354385
90-94	0.025185780482779717
95-99	0.03803525062289409
100-101	0.033982439953265345
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.562866E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	6.635364017904307
#Duplication Level	Relative count
1	100.0
2	3.523036677166195
3	0.6919011264748278
4	0.4938337515348887
5	0.3715765308846298
6	0.2909615076610966
7	0.23970957236666487
8	0.1927286316801025
9	0.15001868560140943
10++	0.8210987133628744
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCCAG	2799930	3.5830476	3.9058285	25-29
CTGGG	2746150	3.4906414	3.666221	85-89
CCAGG	2498515	3.1747437	3.4193654	25-29
GGAGG	2530570	3.170207	3.4490097	70-74
AAAAA	10973615	3.1589468	4.227095	1
GGGGG	1739460	3.147407	4.061918	95-97
CCTCC	2407805	3.1263556	3.561009	3
CCTGG	2416155	3.0930324	3.3282518	3
TTTTT	10330145	3.086482	3.6197288	10-14
>>END_MODULE