##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename ZL6_CTTGTA_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15628660 Filtered Sequences 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.23660301011091 34.0 33.0 34.0 31.0 34.0 2 33.38043133576391 34.0 34.0 34.0 31.0 34.0 3 33.40766674814092 34.0 34.0 34.0 31.0 34.0 4 36.649268011461 37.0 37.0 37.0 35.0 37.0 5 36.592921849985856 37.0 37.0 37.0 35.0 37.0 6 36.583362745110584 37.0 37.0 37.0 35.0 37.0 7 36.58621366131197 37.0 37.0 37.0 35.0 37.0 8 36.58242875588822 37.0 37.0 37.0 35.0 37.0 9 38.44702597663523 39.0 39.0 39.0 37.0 39.0 10-14 38.75168123178827 39.4 39.2 39.4 37.2 39.4 15-19 40.03503501899715 41.0 40.0 41.0 38.0 41.0 20-24 39.97849223157967 41.0 40.0 41.0 38.0 41.0 25-29 39.82190078995896 41.0 40.0 41.0 38.0 41.0 30-34 39.66670671701861 41.0 40.0 41.0 37.8 41.0 35-39 39.46961538609196 41.0 40.0 41.0 37.0 41.0 40-44 39.28388056301692 41.0 39.2 41.0 36.4 41.0 45-49 39.139473838448076 40.4 39.0 41.0 36.0 41.0 50-54 39.10222125249381 40.6 39.0 41.0 35.4 41.0 55-59 38.80174886394611 40.0 38.4 41.0 35.0 41.0 60-64 38.334353130722654 40.0 37.4 41.0 34.6 41.0 65-69 37.67575003871093 39.2 36.2 41.0 34.0 41.0 70-74 37.19369748910015 38.4 35.2 40.4 34.0 41.0 75-79 36.058918307775585 36.4 34.8 39.2 32.8 40.2 80-84 35.66441369893516 35.8 35.0 37.8 33.0 39.4 85-89 34.905315657260445 35.0 35.0 36.6 33.0 38.0 90-94 34.35659673957972 35.0 35.0 35.8 32.8 36.8 95-99 34.03318349749755 35.0 35.0 35.0 32.0 36.0 100-101 33.700135424278216 35.0 34.0 35.0 31.5 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 0.0 10 2.0 11 9.0 12 22.0 13 100.0 14 414.0 15 1372.0 16 3466.0 17 6564.0 18 9868.0 19 12339.0 20 14339.0 21 16538.0 22 19490.0 23 23032.0 24 28517.0 25 34698.0 26 41494.0 27 50422.0 28 61400.0 29 76827.0 30 94560.0 31 120000.0 32 155356.0 33 210919.0 34 322242.0 35 555018.0 36 1158835.0 37 2988890.0 38 6272704.0 39 3304863.0 40 44358.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.118804529244517 31.075252476940594 29.603948644333116 20.20199434948177 2 20.227025556933167 30.2718681758189 30.46557933048034 19.03552693676759 3 20.293442869981327 29.344376579470964 30.282433963356752 20.079746587190954 4 20.221672515881696 29.24449462871722 30.36281386340109 20.171018991999993 5 20.50799620696848 29.58270894625643 30.240398089151597 19.668896757623493 6 20.500567547057777 29.693518190299105 29.595640317212098 20.210273945431023 7 20.565992221981926 29.949266283865665 29.808064159051384 19.676677335101026 8 20.050580152105173 29.84163709492688 29.99978245095869 20.10800030200926 9 20.022055633688364 29.633647414429642 30.26928732213766 20.07500962974433 10-14 20.0859769673784 29.803877888166774 30.02964913644369 20.080496008011135 15-19 20.101497665602576 29.650395905828024 30.083624297471363 20.164482131098037 20-24 20.225682981318556 29.536714259821718 29.924414484743007 20.313188274116722 25-29 20.384636659646866 29.50862647788345 29.727263114441836 20.37947374802785 30-34 20.48162398326418 29.490206463335163 29.643779225543714 20.38439032785695 35-39 20.57841503073137 29.41315111549669 29.573745721068896 20.434688132703045 40-44 20.58993526818746 29.419332687002097 29.530020782722303 20.460711262088136 45-49 20.631410070845295 29.335955916094264 29.4948282427136 20.537805770346843 50-54 20.580587730682183 29.38591200342522 29.50548815024258 20.528012115650018 55-59 20.639818539736712 29.32887115262483 29.476613666772 20.554696640866457 60-64 20.60929784685909 29.342333098657235 29.45708388371888 20.59128517076479 65-69 20.635220617132568 29.332063724192874 29.404412077498314 20.628303581176237 70-74 20.667609435768064 29.363614473779243 29.380123961563903 20.58865212888879 75-79 20.66399419281034 29.325640945366526 29.412286992042098 20.598077869781036 80-84 20.65895491246488 29.347747077026526 29.405753775074423 20.587544235434173 85-89 20.623508000456095 29.3427053719426 29.393123260121783 20.64066336747952 90-94 20.614983670663786 29.311512078038827 29.375581516418837 20.697922734878556 95-99 20.635860307456106 29.341849701559337 29.335401966676883 20.686888024307677 100-101 20.71421215345806 29.306834378589503 29.230499309097237 20.748454158855196 >>END_MODULE >>Per base GC content pass #Base %GC 1 39.320798878726286 2 39.26255249370076 3 40.37318945717228 4 40.392691507881686 5 40.17689296459198 6 40.7108414924888 7 40.24266955708295 8 40.15858045411443 9 40.0970652634327 10-14 40.16647297538953 15-19 40.26597979670061 20-24 40.53887125543528 25-29 40.764110407674714 30-34 40.86601431112113 35-39 41.013103163434415 40-44 41.0506465302756 45-49 41.169215841192134 50-54 41.108599846332204 55-59 41.19451518060317 60-64 41.200583017623885 65-69 41.26352419830881 70-74 41.256261564656846 75-79 41.26207206259138 80-84 41.246499147899044 85-89 41.26417136793562 90-94 41.31290640554234 95-99 41.32274833176378 100-101 41.46266631231326 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 244.0 1 228.5 2 246.0 3 344.5 4 479.0 5 560.0 6 727.5 7 1038.0 8 1400.5 9 1811.5 10 2231.0 11 2921.5 12 4065.0 13 5359.0 14 6844.5 15 8968.0 16 11891.0 17 16195.0 18 22901.0 19 32751.5 20 46527.5 21 64176.0 22 86138.0 23 112608.0 24 143486.0 25 178526.5 26 217437.5 27 259159.5 28 301542.5 29 344223.5 30 386292.5 31 426549.5 32 466101.0 33 504278.5 34 538936.5 35 570835.0 36 600943.5 37 623704.0 38 634182.5 39 631356.0 40 616868.0 41 592184.5 42 558603.0 43 521119.0 44 486449.5 45 459079.0 46 436552.5 47 419094.5 48 404962.0 49 388584.0 50 368019.5 51 344313.5 52 318066.5 53 292609.5 54 270181.0 55 250256.0 56 232149.5 57 213697.0 58 192961.0 59 170000.5 60 146349.0 61 123363.5 62 102504.0 63 84627.5 64 70237.5 65 58422.0 66 48183.5 67 39368.0 68 31961.5 69 25515.0 70 20232.0 71 16186.0 72 13010.0 73 10498.5 74 8547.5 75 7023.5 76 5861.0 77 4967.5 78 4247.0 79 3521.0 80 2831.0 81 2159.5 82 1605.0 83 1203.5 84 838.0 85 516.0 86 306.0 87 196.5 88 132.5 89 98.5 90 76.5 91 61.0 92 51.5 93 35.5 94 24.0 95 20.0 96 17.5 97 10.5 98 4.5 99 5.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.19925060753769E-5 2 0.011421324668909555 3 0.002591392992105529 4 0.005899418120299501 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 5.8866211178693505E-5 15-19 2.840934539493469E-4 20-24 0.0022279581230892475 25-29 0.011637594009979102 30-34 0.007353157596364628 35-39 0.030220121238801023 40-44 0.02466110338314353 45-49 0.0266446387598169 50-54 0.027554505632600618 55-59 0.02414538418520846 60-64 0.054700786887679424 65-69 0.09620658456963041 70-74 0.015956582330154984 75-79 0.017841580788116193 80-84 0.002974023364767037 85-89 0.01320266740718654 90-94 0.015925869524322622 95-99 0.009384041882029555 100-101 0.0012445084863321617 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.562866E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 6.576321251796259 #Duplication Level Relative count 1 100.0 2 3.463009250357837 3 0.6991176910423209 4 0.5250058749385802 5 0.40537076203294237 6 0.2766561985942874 7 0.23499754320750282 8 0.2034865090046786 9 0.15648700036317803 10++ 0.8529342647781409 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position CCCAG 2821550 3.6084883 3.9488034 25-29 CTGGG 2759935 3.4945424 3.7550786 1 CCAGG 2514330 3.208032 3.432748 25-29 GGAGG 2482835 3.1529858 3.4145877 70-74 CCTCC 2449225 3.1230829 3.4134126 95-97 CCTGG 2435075 3.0904727 3.2656522 3 >>END_MODULE