##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL5_CAGATC_L008_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	9436412	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.719900318044616	34.0	31.0	34.0	31.0	34.0
2	32.860316823809725	34.0	33.0	34.0	31.0	34.0
3	32.86507562408254	34.0	33.0	34.0	31.0	34.0
4	36.18018469308038	37.0	37.0	37.0	35.0	37.0
5	36.16341211045045	37.0	37.0	37.0	35.0	37.0
6	36.190312377204386	37.0	37.0	37.0	35.0	37.0
7	36.19259343487758	37.0	37.0	37.0	35.0	37.0
8	36.18009684189287	37.0	37.0	37.0	35.0	37.0
9	37.96379322988441	39.0	39.0	39.0	37.0	39.0
10-14	38.234475434095074	39.4	39.2	39.4	37.0	39.4
15-19	39.42723511860228	41.0	40.0	41.0	37.0	41.0
20-24	39.336478759087676	41.0	40.0	41.0	37.0	41.0
25-29	39.16177131731849	41.0	39.2	41.0	36.0	41.0
30-34	38.95081842547782	40.6	39.0	41.0	35.8	41.0
35-39	38.70013121512711	40.0	38.6	41.0	35.0	41.0
40-44	38.61083526238575	40.0	38.6	41.0	35.0	41.0
45-49	38.471556858687386	40.0	38.0	41.0	34.6	41.0
50-54	37.842704663594596	39.6	37.8	40.4	33.8	40.8
55-59	38.44181313829875	40.0	38.4	41.0	34.6	41.0
60-64	38.082813616022705	40.0	37.4	41.0	34.0	41.0
65-69	37.51440312271232	39.4	36.2	41.0	33.4	41.0
70-74	36.75216253804942	38.2	35.0	40.4	32.6	41.0
75-79	35.86836339913942	36.8	35.0	39.2	31.8	40.8
80-84	34.94589019640092	35.6	35.0	37.6	31.0	39.2
85-89	34.17010397596035	35.0	34.0	36.4	30.6	37.8
90-94	33.62930753765308	35.0	34.0	35.6	30.0	36.6
95-99	33.23759695952233	35.0	34.0	35.0	29.2	36.0
100-101	32.60057132944174	34.5	33.0	35.0	27.5	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	14.0
4	42.0
5	104.0
6	392.0
7	2453.0
8	5340.0
9	4793.0
10	4730.0
11	5558.0
12	6670.0
13	8598.0
14	10504.0
15	11913.0
16	13635.0
17	15467.0
18	17263.0
19	18723.0
20	20175.0
21	21826.0
22	23575.0
23	25984.0
24	28597.0
25	32809.0
26	37847.0
27	43516.0
28	51754.0
29	60393.0
30	72502.0
31	91367.0
32	119311.0
33	163036.0
34	243600.0
35	399967.0
36	778712.0
37	1847200.0
38	3568293.0
39	1664026.0
40	15723.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.771008486706677	32.65429213763472	30.22351036053706	19.35118901512154
2	20.666488908573793	32.66412078047257	28.319918748854544	18.34947156209909
3	20.53391335634078	30.884697728134793	29.106220496427966	19.475168419096462
4	20.433969913337123	29.725127355247366	29.511392442121327	20.329510289294184
5	20.32025076383129	29.994577431530928	30.09617767783198	19.588994126805805
6	20.51165848698332	30.115811001398473	29.060360465796713	20.312170045821492
7	20.56883820000528	30.11306325350463	29.696642166466475	19.621456380023616
8	19.971483152764446	30.309926964938317	29.843986404615876	19.87460347768136
9	19.948262451322428	29.990124528068225	30.172249266496515	19.889363754112836
10-14	19.993311120048748	30.12858642257033	29.984079134047047	19.89402332333387
15-19	20.038413373596388	29.886481871185204	30.03816682962665	20.036937925591758
20-24	20.216918496336497	29.698588079363002	29.859350176366867	20.225143247933634
25-29	20.401118267052762	29.705039525010996	29.623126961505967	20.270715246430278
30-34	20.550044185240008	29.598470407059768	29.53087677204899	20.32060863565123
35-39	20.586481245853744	29.51790423303461	29.464566327941398	20.431048193170252
40-44	20.64287093490807	29.51732612921069	29.413611965632075	20.42619097024916
45-49	20.6544008855669	29.437013935433804	29.37092628371246	20.537658895286835
50-54	20.65240706541594	29.450306867561416	29.384080439709898	20.51320562731275
55-59	20.709181602954477	29.445833932311444	29.32488699986388	20.520097464870197
60-64	20.666288541243592	29.44951289957624	29.30612373656267	20.5780748226175
65-69	20.67456488819691	29.427646313772605	29.28947361893504	20.60831517909544
70-74	20.724302697807474	29.452409938546875	29.247698340781948	20.575589022863703
75-79	20.71029298051865	29.423874451454424	29.271604079901103	20.594228488125818
80-84	20.722733517683558	29.461638619161285	29.246876006689803	20.56875185646535
85-89	20.681771673572825	29.420164840332397	29.250606339925366	20.647457146169412
90-94	20.66039641409621	29.39099150936223	29.266875944612238	20.68173613192933
95-99	20.697394552490902	29.40943797583865	29.20940579123557	20.683761680434877
100-101	20.77494244433635	29.389813210830674	29.125898789508632	20.709345555324347
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	37.12219750182822
2	39.01596047067288
3	40.009081775437245
4	40.7634802026313
5	39.90924489063709
6	40.82382853280481
7	40.1902945800289
8	39.84608663044581
9	39.837626205435264
10-14	39.88733444338262
15-19	40.07535129918814
20-24	40.44206174427013
25-29	40.67183351348304
30-34	40.87065282089124
35-39	41.01752943902399
40-44	41.069061905157234
45-49	41.19205978085373
50-54	41.16561269272869
55-59	41.22927906782468
60-64	41.24436336386108
65-69	41.28288006729235
70-74	41.29989172067118
75-79	41.30452146864447
80-84	41.29148537414891
85-89	41.329228819742234
90-94	41.34213254602553
95-99	41.38115623292578
100-101	41.484287999660694
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	120.0
1	137.0
2	167.5
3	227.5
4	303.5
5	391.0
6	527.0
7	695.5
8	945.0
9	1166.0
10	1422.5
11	1825.5
12	2361.0
13	2996.0
14	3769.0
15	4981.5
16	6825.5
17	9509.5
18	13623.5
19	19662.0
20	28143.0
21	39129.0
22	52263.5
23	68224.0
24	87071.0
25	108820.5
26	132366.0
27	156734.5
28	182666.0
29	209205.5
30	234304.0
31	258081.5
32	282265.0
33	305339.5
34	326283.0
35	345940.0
36	363459.0
37	377265.0
38	384349.5
39	383034.0
40	374681.5
41	359481.5
42	338600.5
43	316499.0
44	295951.5
45	277920.0
46	264434.5
47	253739.0
48	243660.0
49	232586.5
50	219398.5
51	205125.5
52	190172.0
53	175291.0
54	161526.5
55	149732.5
56	138960.0
57	127645.0
58	114776.5
59	101143.0
60	87088.0
61	73185.5
62	60786.5
63	50410.5
64	41909.5
65	34644.5
66	28460.0
67	23428.5
68	19120.5
69	15347.0
70	12340.5
71	9823.5
72	7889.5
73	6344.5
74	5171.5
75	4362.0
76	3576.5
77	2984.5
78	2521.0
79	2132.0
80	1768.5
81	1351.0
82	1064.0
83	789.5
84	528.0
85	380.0
86	266.5
87	194.0
88	139.0
89	110.5
90	87.0
91	68.0
92	61.5
93	51.5
94	43.0
95	38.5
96	29.0
97	23.0
98	17.5
99	8.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09747348886419965
2	0.08337914876968068
3	0.09261994919255327
4	0.09374325750083824
5	0.07737050904517521
6	0.03480136306045136
7	0.013257157487400932
8	0.043374536847267796
9	0.05260474002194902
10-14	0.04915639546047799
15-19	0.021082165551906805
20-24	0.015647896679373476
25-29	0.00785256090980343
30-34	0.0096244208074001
35-39	0.011612464568100672
40-44	0.025501218047707115
45-49	0.024102381286446584
50-54	0.03518074454570233
55-59	0.03899787334423296
60-64	0.02654822616901424
65-69	0.02872066204824461
70-74	0.030178843399376796
75-79	0.04495564627741985
80-84	0.04778935044379156
85-89	0.032741258012049496
90-94	0.04487722664080373
95-99	0.035288836477254275
100-101	0.056425047994937055
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	9436412.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	5.937176217422498
#Duplication Level	Relative count
1	100.0
2	3.2023004453670705
3	0.7800543337234488
4	0.4903803890209868
5	0.3627543914464103
6	0.258958974331817
7	0.19911773895452595
8	0.13133297675724054
9	0.0910857742026023
10++	0.3977059094543856
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCCAG	1695945	3.5997813	3.9122376	25-29
CTGGG	1660065	3.4995422	3.6845202	85-89
CCAGG	1514365	3.1924126	3.439897	25-29
GGAGG	1522300	3.165461	3.4662902	70-74
AAAAA	6530365	3.114264	4.239692	1
CCTCC	1447015	3.1137724	3.4517603	3
CCTGG	1461340	3.1017964	3.2829425	70-74
GGGGG	1027610	3.0877683	4.0836396	95-97
TTTTT	6199055	3.059224	3.6462307	10-14
>>END_MODULE