##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename ZL5_CAGATC_L008_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9436412 Filtered Sequences 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.231059856225016 34.0 33.0 34.0 31.0 34.0 2 33.36689093269772 34.0 34.0 34.0 31.0 34.0 3 33.38704011651886 34.0 34.0 34.0 31.0 34.0 4 36.63015974715814 37.0 37.0 37.0 35.0 37.0 5 36.569100204611665 37.0 37.0 37.0 35.0 37.0 6 36.55349225955798 37.0 37.0 37.0 35.0 37.0 7 36.55642155090303 37.0 37.0 37.0 35.0 37.0 8 36.560661721849364 37.0 37.0 37.0 35.0 37.0 9 38.40500361790053 39.0 39.0 39.0 37.0 39.0 10-14 38.70897184226378 39.4 39.2 39.4 37.2 39.4 15-19 39.975170223597694 41.0 40.0 41.0 38.0 41.0 20-24 39.907646423237985 41.0 40.0 41.0 38.0 41.0 25-29 39.739182011128804 41.0 40.0 41.0 38.0 41.0 30-34 39.571037826665474 41.0 40.0 41.0 37.2 41.0 35-39 39.36163639315451 41.0 39.4 41.0 36.8 41.0 40-44 39.16195958802986 40.4 39.0 41.0 36.0 41.0 45-49 39.01664940021695 40.0 39.0 41.0 35.4 41.0 50-54 38.98701913396744 40.0 39.0 41.0 35.0 41.0 55-59 38.6741659223866 40.0 38.2 41.0 35.0 41.0 60-64 38.20597551272666 40.0 37.4 41.0 34.2 41.0 65-69 37.54700553557856 39.0 36.0 41.0 33.6 41.0 70-74 37.077706590174316 38.2 35.2 40.2 33.6 41.0 75-79 35.948857955756914 36.4 34.8 39.2 32.4 40.2 80-84 35.568528292321275 35.8 35.0 37.8 33.0 39.4 85-89 34.78853123411737 35.0 35.0 36.6 32.6 38.0 90-94 34.23920390504357 35.0 34.8 35.8 32.0 36.8 95-99 33.90483592704514 35.0 34.0 35.0 32.0 36.0 100-101 33.549787514576515 35.0 34.0 35.0 30.5 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 4.0 11 4.0 12 15.0 13 66.0 14 261.0 15 817.0 16 1923.0 17 3824.0 18 5932.0 19 7445.0 20 8735.0 21 10342.0 22 12440.0 23 14556.0 24 17402.0 25 21811.0 26 26637.0 27 32551.0 28 40239.0 29 49740.0 30 62150.0 31 79177.0 32 103798.0 33 143052.0 34 218755.0 35 372624.0 36 759315.0 37 1876554.0 38 3709949.0 39 1833626.0 40 22668.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 18.90919557380774 31.026520019940246 30.39212216114297 19.672162245109043 2 19.987310189354147 30.362168298004317 30.5780773612146 19.072444151426936 3 20.289332428469635 29.459120691211872 30.382639079344987 19.868907800973506 4 20.309265852317594 29.28276128681113 30.452803459620032 19.95516940125124 5 20.530716547772606 29.606199898859863 30.310429430169012 19.552654123198522 6 20.551794474425236 29.766705819966315 29.58950923295846 20.091990472649986 7 20.52531195119501 29.980484107730774 29.887313101632273 19.60689083944194 8 20.122976826361544 29.971646002739178 29.925092291434495 19.98028487946478 9 20.052060041464912 29.785272198797596 30.185869374927677 19.97679838480982 10-14 20.04862888955346 29.88710696469382 30.079288997480596 19.98497514827212 15-19 20.044263975339646 29.723838905190835 30.140180205865935 20.091716913603584 20-24 20.190342341532748 29.571515136229348 29.977904629811942 20.260237892425963 25-29 20.357162358736392 29.53713900430629 29.769723063805483 20.335975573151835 30-34 20.490446328622962 29.501821234712434 29.67104035441691 20.33669208224769 35-39 20.570993432541133 29.419418471428312 29.59405008448231 20.415538011548247 40-44 20.61249310187004 29.403054651886016 29.528536863841804 20.45591538240214 45-49 20.636422772679495 29.336540564681705 29.50481273369574 20.522223928943053 50-54 20.604481721289787 29.38223426146058 29.48690790775541 20.526376109494223 55-59 20.660347146323467 29.329096954077627 29.460512021440888 20.550043878158018 60-64 20.639444007383336 29.329510801402712 29.45333959919517 20.577705592018777 65-69 20.652801475510095 29.318975482469146 29.412028477877712 20.616194564143047 70-74 20.690003232927292 29.347972903282134 29.370153298928912 20.59187056486166 75-79 20.681863083437772 29.31207847400169 29.403370596924056 20.60268784563648 80-84 20.688426406842495 29.33797389042288 29.384960505956485 20.588639196778136 85-89 20.645025962252582 29.309589249595557 29.38454691367224 20.66083787447962 90-94 20.634229009069436 29.26931038575295 29.378466188605266 20.717994416572346 95-99 20.64951663209585 29.297505256104895 29.33595135048133 20.717026761317925 100-101 20.721477296878753 29.265850732719663 29.272050275002687 20.740621695398893 >>END_MODULE >>Per base GC content pass #Base %GC 1 38.581357818916786 2 39.05975434078108 3 40.15824022944314 4 40.264435253568834 5 40.08337067097113 6 40.643784947075225 7 40.13220279063695 8 40.10326170582633 9 40.02885842627473 10-14 40.033604037825576 15-19 40.13598088894324 20-24 40.45058023395871 25-29 40.693137931888224 30-34 40.82713841087065 35-39 40.98653144408938 40-44 41.068408484272176 45-49 41.15864670162255 50-54 41.13085783078401 55-59 41.21039102448149 60-64 41.21714959940211 65-69 41.268996039653146 70-74 41.281873797788954 75-79 41.28455092907425 80-84 41.277065603620635 85-89 41.3058638367322 90-94 41.35222342564178 95-99 41.36654339341377 100-101 41.462098992277646 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 54.0 1 51.5 2 63.5 3 98.5 4 159.5 5 222.5 6 339.0 7 533.0 8 735.5 9 899.0 10 1091.0 11 1423.5 12 1986.0 13 2662.0 14 3410.5 15 4569.5 16 6288.0 17 8813.0 18 12893.5 19 18933.5 20 27455.0 21 38080.5 22 51197.5 23 67462.5 24 86420.5 25 108092.5 26 131891.0 27 157355.5 28 183078.5 29 208465.5 30 233863.5 31 258380.5 32 282198.0 33 305768.0 34 326753.0 35 345848.0 36 364151.5 37 378243.5 38 385417.5 39 384368.5 40 375566.0 41 360467.5 42 340642.5 43 318551.0 44 296755.5 45 278593.0 46 264169.5 47 253122.0 48 243423.5 49 233064.5 50 221020.0 51 206184.5 52 190302.0 53 175362.0 54 162122.5 55 149682.5 56 138387.0 57 127320.5 58 115183.5 59 101786.0 60 87572.0 61 73417.5 62 60712.5 63 50253.5 64 41682.5 65 34427.0 66 28316.5 67 23329.5 68 18932.0 69 15104.0 70 12106.5 71 9635.0 72 7722.0 73 6169.5 74 4952.5 75 4120.0 76 3416.0 77 2853.0 78 2410.0 79 2011.5 80 1625.5 81 1237.5 82 946.0 83 709.0 84 486.0 85 305.5 86 172.5 87 111.0 88 74.5 89 52.0 90 35.5 91 30.0 92 25.5 93 18.0 94 16.5 95 13.0 96 8.0 97 3.5 98 1.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002331394602100883 2 0.005245637854726987 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 4.8747341680291194E-4 15-19 0.001222922441283827 20-24 4.8747341680291194E-4 25-29 0.01362382227482225 30-34 0.008850821689430263 35-39 0.030089826514569307 40-44 0.017046733440634003 45-49 0.019240363816247108 50-54 0.02669234874441684 55-59 0.019895273754473627 60-64 0.03932850748780363 65-69 0.08044794991994839 70-74 0.030267860284184288 75-79 0.01742187602660842 80-84 0.005652572185275505 85-89 0.01857697607946749 90-94 0.030982114812282465 95-99 0.014660233147938009 100-101 0.0024532629563016116 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9436412.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 5.723705868856054 #Duplication Level Relative count 1 100.0 2 3.0877163279666893 3 0.7460041162496627 4 0.5068595343029624 5 0.3603041157205818 6 0.26612770955573073 7 0.18306200299459807 8 0.13967736644674536 9 0.09682181083240303 10++ 0.42961371801044407 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position CCCAG 1697755 3.6126564 4.015072 25-29 CTGGG 1658465 3.4807937 3.6619885 85-89 CCAGG 1516260 3.2136462 3.4858296 25-29 GGAGG 1493415 3.1401494 3.403537 65-69 CCTCC 1463350 3.108151 3.4088943 90-94 CCTGG 1467070 3.091364 3.2928762 85-89 >>END_MODULE