FastQCFastQC Report
Thu 31 Jul 2014
ZL5_CAGATC_L007_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename ZL5_CAGATC_L007_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9340932
Filtered Sequences 0
Sequence length 101
%GC 40

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CCCAG 1688390 3.6121569 3.974485 25-29
CTGGG 1643835 3.495128 3.7273588 85-89
CCAGG 1508815 3.2071579 3.445896 25-29
GGAGG 1507455 3.1630743 3.4600632 70-74
AAAAA 6484175 3.1300828 4.320057 1
CCTCC 1432940 3.106431 3.473019 70-74
CCTGG 1448345 3.0994632 3.3106866 65-69
GGGGG 1019430 3.0886126 4.1241837 95-97
TTTTT 6149585 3.0704212 3.6685016 10-14