##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL5_CAGATC_L007_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	9340932	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.762594674706975	34.0	31.0	34.0	31.0	34.0
2	32.90035052176806	34.0	33.0	34.0	31.0	34.0
3	32.90707907947515	34.0	33.0	34.0	31.0	34.0
4	36.212110847183126	37.0	37.0	37.0	35.0	37.0
5	36.20568600649271	37.0	37.0	37.0	35.0	37.0
6	36.23262550246592	37.0	37.0	37.0	35.0	37.0
7	36.230247795402	37.0	37.0	37.0	35.0	37.0
8	36.22181897909117	37.0	37.0	37.0	35.0	37.0
9	38.00223971226854	39.0	39.0	39.0	37.0	39.0
10-14	38.28081673220616	39.4	39.2	39.4	37.0	39.4
15-19	39.488879096861	41.0	40.0	41.0	37.0	41.0
20-24	39.393243843333835	41.0	40.0	41.0	37.0	41.0
25-29	39.23404848681052	41.0	39.8	41.0	36.6	41.0
30-34	39.02672063130318	41.0	39.0	41.0	36.0	41.0
35-39	38.78861254958285	40.0	39.0	41.0	35.2	41.0
40-44	38.73027740700821	40.0	39.0	41.0	35.0	41.0
45-49	38.580741857450626	40.0	38.4	41.0	35.0	41.0
50-54	37.92438934359012	39.6	38.0	40.4	34.0	40.8
55-59	38.51642401422042	40.0	38.6	41.0	34.8	41.0
60-64	38.16858746000934	40.0	37.4	41.0	34.0	41.0
65-69	37.60251028484095	39.4	36.4	41.0	33.6	41.0
70-74	36.85848178747045	38.4	35.2	40.4	33.0	41.0
75-79	35.96760479575272	36.8	35.0	39.2	32.0	40.8
80-84	35.058083026404645	35.6	35.0	37.6	31.4	39.2
85-89	34.27882040036262	35.0	34.4	36.4	31.0	37.8
90-94	33.73114665645784	35.0	34.0	35.6	30.6	36.6
95-99	33.34771194137801	35.0	34.0	35.0	30.0	36.0
100-101	32.74467296196996	34.5	33.0	35.0	28.0	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	6.0
5	20.0
6	270.0
7	2143.0
8	5032.0
9	4482.0
10	4341.0
11	5129.0
12	6410.0
13	8174.0
14	10152.0
15	11716.0
16	12841.0
17	14820.0
18	16267.0
19	18035.0
20	19061.0
21	20315.0
22	22075.0
23	24530.0
24	27219.0
25	30914.0
26	35591.0
27	41631.0
28	49347.0
29	55949.0
30	67725.0
31	84362.0
32	110039.0
33	151291.0
34	226796.0
35	373602.0
36	742228.0
37	1801827.0
38	3585825.0
39	1733566.0
40	17198.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.789047541960652	32.62496380139412	30.20660230430442	19.37938635234081
2	20.69184236976567	32.66880317177902	28.27521765855654	18.364136799898773
3	20.59274946017835	30.84242822450752	29.062249001147787	19.502573314166344
4	20.479387038653634	29.716614430674465	29.510977057120936	20.293021473550972
5	20.31806624498355	29.99721058418337	30.07555846161361	19.60916470921947
6	20.498879648116684	30.14298471505783	29.049494725529577	20.308640911295903
7	20.568087098551192	30.081774006091162	29.718199547847295	19.63193934751035
8	19.97674682292413	30.304088985212633	29.85041755181241	19.86874664005082
9	19.981648947732374	30.006465378316722	30.113329365731182	19.898556308219725
10-14	19.986879531242096	30.13072840509744	29.96505534278226	19.917336720878208
15-19	20.038434581383214	29.89368180036072	30.021550786722894	20.046332831533174
20-24	20.246150743934702	29.688722833625874	29.845568905206882	20.21955751723254
25-29	20.414293425293383	29.68073115536739	29.608828127096015	20.296147292243212
30-34	20.56841050521329	29.596456916729757	29.505562179772703	20.32957039828425
35-39	20.59673939469884	29.499938052259395	29.467550925518086	20.435771627523675
40-44	20.64080186882627	29.50929732674484	29.403750849062995	20.446149955365904
45-49	20.686670223777842	29.421404065236263	29.354382180008027	20.537543530977867
50-54	20.663341556696714	29.4501104473479	29.358563735813416	20.527984260141974
55-59	20.73861343199274	29.426604308496255	29.30991778680252	20.524864472708483
60-64	20.677339407493935	29.4315880294029	29.30621873544205	20.584853827661114
65-69	20.68256711478352	29.411714944473633	29.273354922251222	20.632363018491628
70-74	20.729915701592496	29.438957821840383	29.235954070513703	20.595172406053422
75-79	20.719797685379074	29.42198386570112	29.259033281087543	20.59918516783227
80-84	20.730293425970938	29.44295126324971	29.253613027443798	20.573142283335557
85-89	20.678710610200763	29.42051222988107	29.25232467601276	20.648452483905412
90-94	20.676686415471917	29.386487773787657	29.235195677650154	20.70163013309027
95-99	20.718269728950688	29.402556975412036	29.196560087148676	20.682613208488604
100-101	20.78670561715112	29.359819191561304	29.14021269483296	20.71326249645461
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	37.16843389430146
2	39.05597916966444
3	40.095322774344694
4	40.772408512204606
5	39.92723095420302
6	40.807520559412595
7	40.20002644606154
8	39.84549346297495
9	39.8802052559521
10-14	39.9042162521203
15-19	40.084767412916385
20-24	40.465708261167244
25-29	40.71044071753659
30-34	40.89798090349754
35-39	41.032511022222515
40-44	41.08695182419217
45-49	41.22421375475571
50-54	41.191325816838685
55-59	41.263477904701226
60-64	41.262193235155046
65-69	41.314930133275155
70-74	41.32508810764592
75-79	41.318982853211345
80-84	41.3034357093065
85-89	41.327163094106176
90-94	41.37831654856219
95-99	41.400882937439285
100-101	41.49996811360573
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	128.0
1	138.0
2	178.5
3	223.0
4	280.0
5	353.0
6	464.0
7	662.0
8	921.5
9	1148.0
10	1397.0
11	1756.0
12	2330.5
13	3040.5
14	3717.0
15	4825.0
16	6609.5
17	9233.0
18	13299.5
19	19217.0
20	27564.0
21	38210.5
22	51392.5
23	67015.0
24	85198.0
25	106830.0
26	130541.0
27	155233.0
28	181050.5
29	206659.0
30	231009.5
31	254927.0
32	278187.5
33	301166.0
34	322782.5
35	341633.5
36	359465.5
37	373243.5
38	380157.5
39	379486.5
40	370832.0
41	356359.0
42	336731.0
43	313707.0
44	292803.0
45	276202.0
46	261886.5
47	250732.5
48	240690.0
49	229672.5
50	218221.5
51	204408.0
52	188686.5
53	174062.0
54	161233.5
55	149043.0
56	137649.0
57	126397.5
58	114092.0
59	100859.0
60	86925.0
61	72881.0
62	60476.0
63	50235.0
64	41641.5
65	34470.5
66	28301.5
67	23215.0
68	18978.0
69	15197.0
70	12086.0
71	9681.0
72	7773.0
73	6260.5
74	5053.0
75	4250.0
76	3545.5
77	2932.0
78	2438.5
79	2009.5
80	1644.0
81	1307.5
82	1027.0
83	751.5
84	531.5
85	371.0
86	246.0
87	178.5
88	135.0
89	97.5
90	80.0
91	63.0
92	54.0
93	49.5
94	41.5
95	30.5
96	25.0
97	22.5
98	20.5
99	17.0
100	8.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07503533908607835
2	0.08158714783492696
3	0.0879997841757118
4	0.06480081430846515
5	0.060422236239381685
6	0.006027235826146684
7	0.012493400016186821
8	0.012129410641250787
9	0.02096150576837515
10-14	0.020730265459592254
15-19	0.02306622080109351
20-24	0.018325794471044217
25-29	0.0031709897898839217
30-34	0.0048945865359045545
35-39	0.008577302564669136
40-44	0.02256520013206391
45-49	0.020265643728056257
50-54	0.022406757698268224
55-59	0.02576830663150101
60-64	0.02101075138969002
65-69	0.024106802190616523
70-74	0.027646063583376908
75-79	0.029585912840388945
80-84	0.02575974217562016
85-89	0.02091011903309006
90-94	0.024708455216246088
95-99	0.03757012683530937
100-101	0.03311768033425359
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	9340932.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	5.852102850308948
#Duplication Level	Relative count
1	100.0
2	3.17567853664733
3	0.7621628487953592
4	0.5081085658635729
5	0.3371512046407249
6	0.23552949146801033
7	0.1725452004911716
8	0.13761273658805098
9	0.1143244273193039
10++	0.4128382097641529
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCCAG	1688390	3.6121569	3.974485	25-29
CTGGG	1643835	3.495128	3.7273588	85-89
CCAGG	1508815	3.2071579	3.445896	25-29
GGAGG	1507455	3.1630743	3.4600632	70-74
AAAAA	6484175	3.1300828	4.320057	1
CCTCC	1432940	3.106431	3.473019	70-74
CCTGG	1448345	3.0994632	3.3106866	65-69
GGGGG	1019430	3.0886126	4.1241837	95-97
TTTTT	6149585	3.0704212	3.6685016	10-14
>>END_MODULE