##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL5_CAGATC_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	9340932	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.23436301645275	34.0	33.0	34.0	31.0	34.0
2	33.380627757487154	34.0	34.0	34.0	31.0	34.0
3	33.409621973481876	34.0	34.0	34.0	31.0	34.0
4	36.65083837458618	37.0	37.0	37.0	35.0	37.0
5	36.59343318204222	37.0	37.0	37.0	35.0	37.0
6	36.58330400007194	37.0	37.0	37.0	35.0	37.0
7	36.585285279884275	37.0	37.0	37.0	35.0	37.0
8	36.58192758495619	37.0	37.0	37.0	35.0	37.0
9	38.44293010590378	39.0	39.0	39.0	37.0	39.0
10-14	38.74674822598002	39.4	39.2	39.4	37.2	39.4
15-19	40.02866934477201	41.0	40.0	41.0	38.0	41.0
20-24	39.97142310852921	41.0	40.0	41.0	38.0	41.0
25-29	39.815226981633096	41.0	40.0	41.0	38.0	41.0
30-34	39.659800692264966	41.0	40.0	41.0	37.8	41.0
35-39	39.46158477548065	41.0	40.0	41.0	37.0	41.0
40-44	39.2777094405569	41.0	39.2	41.0	36.4	41.0
45-49	39.135295835576144	40.2	39.0	41.0	36.0	41.0
50-54	39.100337418150566	40.6	39.0	41.0	35.6	41.0
55-59	38.803541638029266	40.0	38.4	41.0	35.0	41.0
60-64	38.335630042055755	40.0	37.6	41.0	34.6	41.0
65-69	37.67758082384071	39.2	36.2	41.0	34.0	41.0
70-74	37.19810988882052	38.4	35.2	40.4	34.0	41.0
75-79	36.06675046986746	36.4	34.8	39.2	32.8	40.2
80-84	35.67546491078192	35.8	35.0	37.8	33.0	39.4
85-89	34.91347809833108	35.0	35.0	36.6	33.0	38.0
90-94	34.36334911762552	35.0	35.0	35.8	32.8	36.8
95-99	34.03823731935957	35.0	34.8	35.0	32.0	36.0
100-101	33.70475456838782	35.0	34.0	35.0	31.5	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	6.0
11	2.0
12	11.0
13	68.0
14	250.0
15	915.0
16	2078.0
17	3925.0
18	6020.0
19	7156.0
20	8482.0
21	9670.0
22	11152.0
23	13128.0
24	16550.0
25	19725.0
26	24011.0
27	29618.0
28	35945.0
29	44742.0
30	55788.0
31	70437.0
32	92705.0
33	126572.0
34	193917.0
35	335699.0
36	701120.0
37	1807315.0
38	3756617.0
39	1942250.0
40	25055.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.955688789940876	31.04435403233853	30.339627780182965	19.66032939753763
2	20.045733004863134	30.364599566396212	30.51781380519179	19.07185362354887
3	20.303116501238293	29.44262522537125	30.35944097973824	19.89481729365222
4	20.310122219840686	29.219425929260712	30.48409583248308	19.98635601841552
5	20.58414513669514	29.57467199204533	30.275715528172135	19.56546734308739
6	20.547221626278834	29.771493893757068	29.597324977850175	20.08395950211392
7	20.530135536796543	29.97800433618401	29.90240160189583	19.589458525123618
8	20.133076656590585	29.96544670274872	29.910901824357573	19.990574816303127
9	20.07020284485531	29.75491096605778	30.15939951174037	20.015486677346544
10-14	20.066538145526362	29.858833291888487	30.06989798315105	20.004730579434103
15-19	20.059473888293063	29.69806354972419	30.12208237616351	20.120380185819233
20-24	20.196989012843115	29.55283123663647	29.975526522355228	20.27465322816518
25-29	20.36883929843243	29.52448888535993	29.76420978229485	20.342462033912796
30-34	20.492843705952144	29.50280202898688	29.659553429915718	20.344800835145254
35-39	20.58585434343151	29.41646197632085	29.573025058981557	20.424658621266087
40-44	20.615165040682808	29.40043791943998	29.53113613471877	20.453260905158437
45-49	20.642011921571402	29.330339872576456	29.50348516884017	20.524163037011974
50-54	20.60221958634894	29.347387059786872	29.50175933940084	20.54863401446335
55-59	20.659073053687642	29.32349402941871	29.463422099575965	20.554010817317682
60-64	20.6430076981563	29.330566127679543	29.432665356287192	20.59376081787697
65-69	20.67154761355705	29.32119564667624	29.375891233086826	20.631365506679884
70-74	20.708468846984278	29.343933512251503	29.35283552109248	20.594762119671735
75-79	20.692531487815057	29.307380711126896	29.388457493023672	20.61163030803438
80-84	20.695281671885038	29.325897792271842	29.376551644929105	20.602268890914015
85-89	20.655379045847113	29.30378990706196	29.378072754448198	20.662758292642728
90-94	20.632338247666045	29.27190029844561	29.36637688108898	20.72938457279937
95-99	20.652288472987042	29.294151006108248	29.329122837852367	20.724437683052336
100-101	20.727349380067846	29.252093004035352	29.26083420615994	20.759723409736864
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	38.61601818747851
2	39.117586628412
3	40.19793379489051
4	40.296478238256206
5	40.14961247978253
6	40.631181128392754
7	40.11959406192016
8	40.12365147289371
9	40.085689522201854
10-14	40.07126872496046
15-19	40.1798540741123
20-24	40.4716422410083
25-29	40.71130133234522
30-34	40.837644541097404
35-39	41.01051296469759
40-44	41.06842594584125
45-49	41.166174958583376
50-54	41.15085360081229
55-59	41.213083871005324
60-64	41.236768516033266
65-69	41.30291312023694
70-74	41.30323096665601
75-79	41.30416179584944
80-84	41.29755056279906
85-89	41.31813733848984
90-94	41.36172282046541
95-99	41.37672615603938
100-101	41.48707278980471
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	50.0
1	48.5
2	59.5
3	95.0
4	151.0
5	211.0
6	317.0
7	496.0
8	714.5
9	906.5
10	1106.5
11	1440.0
12	1984.0
13	2660.5
14	3382.5
15	4452.0
16	6143.0
17	8796.5
18	12803.5
19	18581.5
20	26885.0
21	37636.5
22	50648.0
23	66455.0
24	85101.0
25	106289.0
26	129775.5
27	155053.5
28	181094.0
29	206490.5
30	231399.5
31	255569.0
32	278674.0
33	301544.5
34	323285.0
35	342497.0
36	359605.5
37	373800.5
38	381087.0
39	380049.0
40	371680.0
41	356297.0
42	336614.5
43	314956.5
44	294022.5
45	276458.5
46	261703.5
47	250645.5
48	242005.0
49	231641.5
50	218807.5
51	204528.5
52	189092.5
53	174450.0
54	161069.5
55	148958.5
56	138039.5
57	126654.0
58	114445.5
59	100901.0
60	86584.0
61	72766.5
62	60512.5
63	50156.0
64	41360.0
65	34099.5
66	28171.0
67	23299.0
68	18933.5
69	15130.5
70	12001.5
71	9453.0
72	7533.5
73	6103.5
74	4967.0
75	3998.5
76	3290.0
77	2795.5
78	2288.5
79	1780.0
80	1462.0
81	1175.5
82	867.5
83	608.0
84	410.5
85	277.5
86	175.5
87	123.5
88	86.0
89	64.5
90	44.5
91	31.0
92	29.5
93	21.0
94	14.5
95	14.5
96	10.5
97	3.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011444254170782958
3	0.0024943977752969406
4	0.005888063418082907
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	4.710450734466325E-5
15-19	2.783448161275556E-4
20-24	0.0022267585290204448
25-29	0.011459241968574443
30-34	0.0071149217230143625
35-39	0.030435935086563094
40-44	0.02483049871254817
45-49	0.027108643976853704
50-54	0.027806647131142802
55-59	0.024374441436893022
60-64	0.05472901419258806
65-69	0.09546798970381114
70-74	0.01570507097150477
75-79	0.01752287673221473
80-84	0.0029997006722669644
85-89	0.013118605295488715
90-94	0.015788574416343036
95-99	0.009031218726354073
100-101	0.001193671038393171
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	9340932.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	5.696868717605927
#Duplication Level	Relative count
1	100.0
2	3.0908638866700873
3	0.7167757259007306
4	0.5311017186747848
5	0.3491306118778466
6	0.2502102718457901
7	0.1846160356748007
8	0.13753630164884867
9	0.1126739701969414
10++	0.4242465920091409
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCCAG	1684560	3.612297	3.9772403	25-29
CTGGG	1654765	3.5021315	3.6714728	75-79
CCAGG	1509900	3.2250829	3.4750013	25-29
GGAGG	1479045	3.13448	3.4095	70-74
CCTGG	1465330	3.1134067	3.3160899	3
CCTCC	1449875	3.1048427	3.469519	95-97
>>END_MODULE