##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL4_GCCAAT_L008_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	15333671	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75366414213531	34.0	31.0	34.0	31.0	34.0
2	32.889665560191034	34.0	33.0	34.0	31.0	34.0
3	32.89741888944924	34.0	33.0	34.0	31.0	34.0
4	36.20740251959234	37.0	37.0	37.0	35.0	37.0
5	36.19326493962209	37.0	37.0	37.0	35.0	37.0
6	36.21811613148606	37.0	37.0	37.0	35.0	37.0
7	36.220704944041124	37.0	37.0	37.0	35.0	37.0
8	36.20815217699663	37.0	37.0	37.0	35.0	37.0
9	38.00068965872556	39.0	39.0	39.0	37.0	39.0
10-14	38.27398002735287	39.4	39.2	39.4	37.0	39.4
15-19	39.47272506368501	41.0	40.0	41.0	37.0	41.0
20-24	39.382368318715066	41.0	40.0	41.0	37.0	41.0
25-29	39.206809171789324	41.0	39.6	41.0	36.2	41.0
30-34	38.996109359591706	40.8	39.0	41.0	36.0	41.0
35-39	38.74458508989791	40.0	39.0	41.0	35.0	41.0
40-44	38.654791458614184	40.0	38.8	41.0	35.0	41.0
45-49	38.511482266705734	40.0	38.0	41.0	34.8	41.0
50-54	37.86471123581561	39.6	37.8	40.4	33.8	40.8
55-59	38.4467338577957	40.0	38.2	41.0	34.6	41.0
60-64	38.08077401686785	40.0	37.4	41.0	34.0	41.0
65-69	37.512906426647604	39.4	36.2	41.0	33.4	41.0
70-74	36.76027325746065	38.2	35.0	40.4	32.6	41.0
75-79	35.88911470710438	36.6	35.0	39.2	32.0	40.8
80-84	34.98067265170878	35.6	35.0	37.6	31.0	39.2
85-89	34.214960827058306	35.0	34.2	36.4	31.0	38.0
90-94	33.68121555497049	35.0	34.0	35.6	30.0	36.6
95-99	33.29373002720614	35.0	34.0	35.0	29.6	36.0
100-101	32.65908861615721	34.5	33.0	35.0	27.5	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	28.0
4	66.0
5	148.0
6	614.0
7	3659.0
8	7841.0
9	7141.0
10	7108.0
11	8480.0
12	10591.0
13	13491.0
14	16481.0
15	18880.0
16	21395.0
17	24391.0
18	27295.0
19	30165.0
20	31958.0
21	34677.0
22	38171.0
23	41340.0
24	46572.0
25	52221.0
26	59582.0
27	70150.0
28	81809.0
29	95520.0
30	116078.0
31	145662.0
32	189973.0
33	260782.0
34	390891.0
35	647618.0
36	1276402.0
37	2989730.0
38	5747299.0
39	2788766.0
40	30696.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.002462734703496	32.36154559156714	30.080921130408676	19.55507054332069
2	20.457376248070208	32.316090258876194	28.772550898106665	18.453982594946932
3	20.39789180297114	30.723203281372996	29.301872046114475	19.577032869541387
4	20.309598941936198	29.75865155222793	29.72738381113031	20.20436569470556
5	20.29175830942773	30.03837101088435	30.147679866030735	19.522190813657183
6	20.421373402373792	30.15907271625323	29.211858397033964	20.207695484339006
7	20.4645286807805	30.195851372841993	29.811508577107215	19.52811136927029
8	19.852830691681525	30.322035785987676	29.990623636174902	19.8345098861559
9	19.819891947957853	30.020375087973843	30.319900495759445	19.839832468308863
10-14	19.90972802788164	30.161886028026963	30.084245628042666	19.84414031604873
15-19	19.968974478372196	29.935753703050654	30.12201955042556	19.97325226815159
20-24	20.144105235754513	29.762023349950095	29.940817098493262	20.15305431580213
25-29	20.317552903188936	29.75584034595223	29.72001801734512	20.206588733513716
30-34	20.457325923160198	29.664046140442206	29.62816067198677	20.250467264410823
35-39	20.5026536849616	29.596279171795764	29.567495881961094	20.333571261281545
40-44	20.557354358749656	29.587050234783774	29.505067862989844	20.350527543476733
45-49	20.569090515872578	29.51375343741512	29.47559403789683	20.441562008815474
50-54	20.55011938124024	29.542281381640002	29.473049147931395	20.434550089188367
55-59	20.604566133757032	29.523435704923372	29.435134367711107	20.436863793608488
60-64	20.56397104897698	29.51872960892823	29.42260124907375	20.494698093021043
65-69	20.575000260938545	29.51858534777889	29.398740188710754	20.50767420257181
70-74	20.61115944774929	29.53280887261839	29.364764722664006	20.491266956968314
75-79	20.596674358650574	29.509768050532603	29.39400008030435	20.499557510512478
80-84	20.613458107611628	29.549721299068167	29.37712019241773	20.45970040090247
85-89	20.577498007483655	29.525425239227545	29.362450221341053	20.53462653194775
90-94	20.57246004440337	29.512595091827603	29.35146257879354	20.563482284975485
95-99	20.61177361274614	29.540526405228455	29.303055807058442	20.544644174966958
100-101	20.702580177340803	29.524781790553188	29.226586275215112	20.546051756890897
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	37.55753327802418
2	38.91135884301714
3	39.97492467251253
4	40.51396463664176
5	39.81394912308492
6	40.6290688867128
7	39.992640050050795
8	39.68734057783742
9	39.65972441626671
10-14	39.75386834393037
15-19	39.94222674652379
20-24	40.297159551556646
25-29	40.52414163670265
30-34	40.70779318757102
35-39	40.83622494624314
40-44	40.907881902226386
45-49	41.010652524688055
50-54	40.9846694704286
55-59	41.04142992736552
60-64	41.058669141998024
65-69	41.08267446351036
70-74	41.102426404717605
75-79	41.096231869163056
80-84	41.0731585085141
85-89	41.112124539431406
90-94	41.13594232937886
95-99	41.1564177877131
100-101	41.2486319342317
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	285.0
1	336.0
2	437.0
3	589.0
4	741.0
5	856.5
6	1116.0
7	1492.0
8	2035.0
9	2716.5
10	3618.5
11	5042.5
12	7035.5
13	9326.0
14	11840.5
15	15134.0
16	19170.5
17	24322.0
18	31789.5
19	41902.0
20	55149.5
21	72348.5
22	93715.5
23	118803.5
24	148023.5
25	181533.5
26	219117.5
27	258364.5
28	298662.5
29	340043.0
30	379616.0
31	417568.5
32	454706.5
33	490627.0
34	523842.0
35	554288.5
36	582587.5
37	603798.5
38	612986.5
39	611052.5
40	598917.5
41	575787.5
42	544927.5
43	510776.5
44	477430.5
45	450273.0
46	428753.5
47	410560.0
48	394320.5
49	376855.5
50	356518.0
51	332834.0
52	307697.5
53	283965.5
54	262217.5
55	242681.0
56	224232.5
57	205912.0
58	186130.0
59	163843.0
60	140665.0
61	118304.5
62	98522.0
63	81782.5
64	68069.5
65	56311.5
66	46201.0
67	37775.5
68	30407.5
69	24548.0
70	19523.0
71	15291.5
72	12343.0
73	10079.0
74	8094.5
75	6534.0
76	5395.0
77	4569.5
78	3903.0
79	3272.0
80	2676.5
81	2113.0
82	1657.5
83	1230.0
84	873.5
85	624.5
86	429.5
87	309.0
88	237.0
89	168.0
90	137.5
91	115.5
92	85.5
93	67.0
94	62.5
95	59.5
96	40.5
97	32.0
98	27.0
99	12.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09735437782641873
2	0.08294165174145188
3	0.09280230415795408
4	0.09370228433882533
5	0.07792002319601092
6	0.03561443309954935
7	0.013336662825229523
8	0.044568583739666774
9	0.052779272491238406
10-14	0.04975586081115214
15-19	0.021680392125277764
20-24	0.01621399076581205
25-29	0.008702417053294022
30-34	0.010171080362947659
35-39	0.012121037421502
40-44	0.026308116301699706
45-49	0.024555111427654867
50-54	0.03620007237666701
55-59	0.04015215925788417
60-64	0.027180705781413985
65-69	0.028636325900040505
70-74	0.030026730063531428
75-79	0.045515519408235644
80-84	0.047932422705560855
85-89	0.03302535968066616
90-94	0.04504466021215663
95-99	0.03504314133256152
100-101	0.05646723475415639
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.5333671E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	6.722890005828219
#Duplication Level	Relative count
1	100.0
2	3.5246807760706216
3	0.6558042413658672
4	0.5110787125302402
5	0.37917020469850626
6	0.289985100213083
7	0.22643403773544601
8	0.19545957030937083
9	0.1692914857597556
10++	0.8566042370935268
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCCAG	2648740	3.510265	3.816068	25-29
CTGGG	2593815	3.4136071	3.5656037	85-89
GGGGG	1760930	3.3340843	4.274913	95-97
AAAAA	11084280	3.2174935	4.2449	1
GGAGG	2444165	3.1803885	3.4553168	70-74
TTTTT	10492420	3.1265028	3.7072392	10-14
CCTCC	2332300	3.1261508	3.440971	70-74
CCAGG	2368030	3.1191587	3.3610063	25-29
CCTGG	2277930	3.0162356	3.2309523	3
>>END_MODULE