##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL4_GCCAAT_L008_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	15333671	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.21872055295826	34.0	33.0	34.0	31.0	34.0
2	33.35346173789694	34.0	34.0	34.0	31.0	34.0
3	33.37395852565247	34.0	34.0	34.0	31.0	34.0
4	36.62200662841925	37.0	37.0	37.0	35.0	37.0
5	36.56171891258134	37.0	37.0	37.0	35.0	37.0
6	36.54527607902896	37.0	37.0	37.0	35.0	37.0
7	36.54855526768508	37.0	37.0	37.0	35.0	37.0
8	36.55325838150564	37.0	37.0	37.0	35.0	37.0
9	38.398398335271445	39.0	39.0	39.0	37.0	39.0
10-14	38.7018368008548	39.4	39.2	39.4	37.2	39.4
15-19	39.964901099025795	41.0	40.0	41.0	38.0	41.0
20-24	39.895639811236336	41.0	40.0	41.0	38.0	41.0
25-29	39.722540949261266	41.0	40.0	41.0	38.0	41.0
30-34	39.5483623458466	41.0	40.0	41.0	37.0	41.0
35-39	39.33496363656165	41.0	39.4	41.0	36.6	41.0
40-44	39.127362208306145	40.4	39.0	41.0	35.8	41.0
45-49	38.973560917017195	40.0	39.0	41.0	35.0	41.0
50-54	38.9337009513247	40.0	39.0	41.0	35.0	41.0
55-59	38.6102431048638	40.0	38.0	41.0	35.0	41.0
60-64	38.13475669329283	40.0	37.2	41.0	34.0	41.0
65-69	37.47571224138043	39.0	35.8	41.0	33.4	41.0
70-74	37.012429482802915	38.2	35.0	40.2	33.0	41.0
75-79	35.888950845495515	36.2	34.8	39.2	32.4	40.2
80-84	35.521126271719275	35.8	35.0	37.8	33.0	39.4
85-89	34.753030021317144	35.0	35.0	36.6	32.4	38.0
90-94	34.2078876610826	35.0	34.8	35.8	32.0	36.8
95-99	33.874131954441964	35.0	34.0	35.0	31.8	36.0
100-101	33.51938909475754	35.0	34.0	35.0	30.5	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	0.0
9	0.0
10	3.0
11	10.0
12	17.0
13	102.0
14	381.0
15	1356.0
16	3418.0
17	6398.0
18	9805.0
19	12964.0
20	15409.0
21	18066.0
22	21514.0
23	25672.0
24	31002.0
25	37691.0
26	45848.0
27	55936.0
28	68754.0
29	85253.0
30	105424.0
31	132913.0
32	173775.0
33	237642.0
34	363779.0
35	619136.0
36	1259614.0
37	3044793.0
38	5927634.0
39	2988897.0
40	40462.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.979785373053673	31.203994338336628	30.01956590827169	19.796654380338012
2	20.020991439528434	30.424249997570584	30.616163904102528	18.93859465879845
3	20.147295451950157	29.540219038219877	30.421886578889033	19.890598930940932
4	20.17870345594346	29.380348645800474	30.488713368116482	19.952234530139588
5	20.45415608564968	29.718571632324704	30.318949715303006	19.508322566722608
6	20.42244808826275	29.86241194297178	29.68118332524547	20.03395664352
7	20.44664320761806	30.123354022660326	29.906974005115934	19.523028764605684
8	19.94788462593204	30.03880805842254	30.052555581765123	19.960751733880294
9	19.940652176507502	29.81864551548028	30.315499791276334	19.925202516735883
10-14	19.95762298846443	29.961247317540625	30.13789211071328	19.943237583281668
15-19	19.9763526575817	29.783843297441624	30.180901295595184	20.058902749381488
20-24	20.108543641513684	29.648570033762734	30.032195716062056	20.210690608661526
25-29	20.282302241843997	29.623874173732688	29.825364701141954	20.26845888328136
30-34	20.41353709371773	29.585333263728568	29.733823864306814	20.26730577824689
35-39	20.4713345146148	29.529715129671906	29.658178804144764	20.34077155156853
40-44	20.514380946652146	29.508211777018	29.605586761740177	20.371820514589672
45-49	20.531898267238834	29.43890406398257	29.59039510064657	20.438802568132026
50-54	20.499475261077837	29.4761782619791	29.568644253104203	20.455702223838856
55-59	20.550691938823956	29.439571318764823	29.549291823052805	20.460444919358416
60-64	20.52985696575197	29.433848413658698	29.523547948011586	20.512746672577737
65-69	20.537483202222173	29.448313623527312	29.486383691432465	20.52781948281805
70-74	20.59075859049026	29.463665982395863	29.446816786470052	20.49875864064382
75-79	20.571883920875482	29.440591541743316	29.47551709703251	20.512007440348686
80-84	20.5754565319514	29.453024906120824	29.479418009759627	20.492100552168147
85-89	20.53921369616883	29.453966022370103	29.453468981965237	20.55335129949583
90-94	20.53525004605375	29.420632426423825	29.453648648759728	20.590468878762696
95-99	20.549751644024642	29.452738660030658	29.39836549956504	20.599144196379665
100-101	20.62293384491767	29.43643537751524	29.31093060542846	20.629700172138627
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	38.776439753391685
2	38.95958609832689
3	40.03789438289109
4	40.13093798608304
5	39.96247865237228
6	40.456404731782754
7	39.96967197222374
8	39.90863635981234
9	39.865854693243385
10-14	39.900860571746094
15-19	40.03525540696319
20-24	40.319234250175214
25-29	40.55076112512536
30-34	40.680842871964614
35-39	40.81210606618333
40-44	40.886201461241825
45-49	40.97070083537086
50-54	40.955177484916696
55-59	41.011136858182375
60-64	41.04260363832971
65-69	41.06530268504022
70-74	41.08951723113408
75-79	41.08389136122417
80-84	41.06755708411954
85-89	41.09256499566466
90-94	41.12571892481645
95-99	41.1488958404043
100-101	41.252634017056295
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	163.0
1	192.0
2	252.5
3	367.5
4	526.0
5	632.5
6	844.0
7	1231.0
8	1743.0
9	2313.5
10	3058.5
11	4367.5
12	6296.0
13	8678.5
14	11200.5
15	14367.0
16	18332.0
17	23504.0
18	30865.0
19	40672.0
20	53980.0
21	71308.5
22	92374.0
23	117481.5
24	146933.5
25	180989.0
26	218105.5
27	257861.5
28	298829.5
29	339874.0
30	379803.0
31	417373.5
32	454981.0
33	490710.5
34	524138.0
35	555987.5
36	583791.5
37	605066.5
38	615229.0
39	613160.5
40	600554.0
41	576994.0
42	545559.5
43	511699.0
44	478653.0
45	450478.5
46	428376.0
47	411072.5
48	395360.0
49	378096.0
50	357718.0
51	334068.5
52	308085.0
53	283601.0
54	262824.5
55	242994.5
56	224609.0
57	206411.0
58	186259.0
59	164382.0
60	141427.0
61	118897.5
62	98859.0
63	82199.0
64	68067.0
65	55781.0
66	45734.0
67	37451.5
68	30229.0
69	24105.5
70	19106.0
71	15094.0
72	12068.5
73	9749.0
74	7875.5
75	6375.5
76	5253.0
77	4431.0
78	3726.5
79	3060.0
80	2439.0
81	1892.0
82	1438.5
83	1029.0
84	700.0
85	439.0
86	277.0
87	195.0
88	125.0
89	88.0
90	64.0
91	55.0
92	42.5
93	29.0
94	20.0
95	15.5
96	12.5
97	9.5
98	7.0
99	3.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002399947148989958
2	0.00491728301722399
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	4.8520670620883936E-4
15-19	0.001259972253219728
20-24	5.099887691603661E-4
25-29	0.013900128677601077
30-34	0.009048061615512684
35-39	0.03044541649550196
40-44	0.017143970286045657
45-49	0.019532178563111208
50-54	0.02701897021267771
55-59	0.020096948734585476
60-64	0.0394895651537065
65-69	0.08118082095279075
70-74	0.030974970051203
75-79	0.018107862102949776
80-84	0.005724656541802677
85-89	0.019078275515367456
90-94	0.031582782753066765
95-99	0.014411421765864156
100-101	0.002540161452531491
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.5333671E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	6.523669819981637
#Duplication Level	Relative count
1	100.0
2	3.392598839598824
3	0.6431846447005322
4	0.49800105685111745
5	0.3928497082984163
6	0.2999749131300407
7	0.23699085663654462
8	0.18521582714612836
9	0.16333153633059158
10++	0.9404907418774586
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCCAG	2656195	3.5212505	3.8688266	25-29
CTGGG	2597105	3.4105847	3.5719316	75-79
GGAGG	2390540	3.142634	3.4407058	70-74
CCAGG	2366205	3.1280687	3.397723	25-29
CCTCC	2351760	3.1143792	3.4346979	95-97
TTTTT	10451340	3.0460932	3.4899435	15-19
CCTGG	2290485	3.0163372	3.1594508	85-89
AAAAA	10108985	3.0035594	3.4580905	1
>>END_MODULE