##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL4_GCCAAT_L007_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	15282102	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79645830135148	34.0	31.0	34.0	31.0	34.0
2	32.92821870970368	34.0	33.0	34.0	31.0	34.0
3	32.93868696858586	34.0	33.0	34.0	31.0	34.0
4	36.240548976835775	37.0	37.0	37.0	35.0	37.0
5	36.235509748593486	37.0	37.0	37.0	35.0	37.0
6	36.26034258899725	37.0	37.0	37.0	35.0	37.0
7	36.25740267929111	37.0	37.0	37.0	35.0	37.0
8	36.24964399530902	37.0	37.0	37.0	35.0	37.0
9	38.03744648478331	39.0	39.0	39.0	37.0	39.0
10-14	38.318193910759135	39.4	39.2	39.4	37.2	39.4
15-19	39.53370706464333	41.0	40.0	41.0	37.0	41.0
20-24	39.43933889461018	41.0	40.0	41.0	37.0	41.0
25-29	39.28215844914528	41.0	40.0	41.0	36.6	41.0
30-34	39.07327070582306	41.0	39.0	41.0	36.0	41.0
35-39	38.83279030594089	40.2	39.0	41.0	35.2	41.0
40-44	38.77230661070055	40.0	39.0	41.0	35.0	41.0
45-49	38.619037276416556	40.0	38.4	41.0	35.0	41.0
50-54	37.946612030203696	39.6	38.0	40.4	34.2	40.8
55-59	38.519338321390606	40.0	38.4	41.0	35.0	41.0
60-64	38.165741270409	40.0	37.4	41.0	34.0	41.0
65-69	37.60168141790966	39.4	36.2	41.0	33.8	41.0
70-74	36.86545091768135	38.4	35.0	40.6	33.0	41.0
75-79	35.98748228483228	36.8	35.0	39.2	32.2	41.0
80-84	35.09271283492284	35.6	35.0	37.6	31.8	39.4
85-89	34.324476541250675	35.0	34.6	36.4	31.0	38.0
90-94	33.7839112446704	35.0	34.0	35.6	30.8	36.6
95-99	33.40431199844105	35.0	34.0	35.0	30.0	36.0
100-101	32.80494698962224	34.5	33.5	35.0	28.5	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	7.0
5	34.0
6	354.0
7	3020.0
8	7394.0
9	6595.0
10	6612.0
11	7745.0
12	9921.0
13	12925.0
14	16019.0
15	18427.0
16	20608.0
17	23418.0
18	26603.0
19	28804.0
20	30605.0
21	33378.0
22	36162.0
23	39096.0
24	43442.0
25	49104.0
26	56406.0
27	66651.0
28	79549.0
29	89882.0
30	108839.0
31	136597.0
32	176948.0
33	242215.0
34	366681.0
35	609265.0
36	1222525.0
37	2936980.0
38	5810196.0
39	2924857.0
40	34236.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.022470535678387	32.364318876457496	30.058656883490777	19.55455370437334
2	20.498545660179904	32.3221335687433	28.739974911287398	18.439345859789395
3	20.408483243953253	30.71254229846388	29.29725148072458	19.581722976858284
4	20.360511666097015	29.762572630735324	29.66816740721534	20.208748295952322
5	20.2893503491201	30.0209237004825	30.155087591519504	19.534638358877903
6	20.416622112969502	30.159772939089933	29.195822889663148	20.227782058277413
7	20.486388157660514	30.15970184730276	29.79591791324525	19.55799208179147
8	19.862946641321656	30.305708137378744	29.996676772472004	19.834668448827596
9	19.88209306891812	30.005955887165392	30.288867072452387	19.8230839714641
10-14	19.914292225602193	30.15596285961536	30.073918962982532	19.855825951799915
15-19	19.970250354970926	29.918899281984025	30.118782327592413	19.992068035452636
20-24	20.16199431670997	29.743267602484895	29.938609262880505	20.15612881792463
25-29	20.33233445764107	29.730852720921312	29.707064606639005	20.229748214798608
30-34	20.474319908761878	29.65433510638994	29.60818729676183	20.263157688086352
35-39	20.519481967702664	29.58360221254565	29.54943103710224	20.347484782649442
40-44	20.563636792760516	29.57323875741702	29.502226445679725	20.360898004142744
45-49	20.57862186777033	29.49728423747181	29.466624914905392	20.45746897985247
50-54	20.56302765278854	29.526741275964717	29.462974797023417	20.44725627422332
55-59	20.629980613119315	29.517678804299795	29.417268175041915	20.43507240753897
60-64	20.567892267053644	29.506986274983486	29.41478104579677	20.510340412166105
65-69	20.596307078053925	29.49766622225788	29.383048576724867	20.522978122963327
70-74	20.623427415623915	29.51955991122653	29.355890175637228	20.501122497512327
75-79	20.61251294094619	29.50147054391784	29.38274674736202	20.50326976777395
80-84	20.611607025508853	29.541232812112682	29.371414406601538	20.475745755776927
85-89	20.571800147835813	29.524048085896144	29.360125974993736	20.544025791274308
90-94	20.575634932994	29.49218424783282	29.35059233394014	20.581588485233045
95-99	20.62857678062571	29.529952952457865	29.294770228637717	20.546700038278708
100-101	20.716281994367215	29.503640312297204	29.222865033718037	20.557212659617544
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	37.57702424005173
2	38.9378915199693
3	39.99020622081154
4	40.56925996204934
5	39.823988707998
6	40.64440417124692
7	40.04438023945199
8	39.69761509014925
9	39.705177040382225
10-14	39.77011817740211
15-19	39.96231839042356
20-24	40.3181231346346
25-29	40.56208267243968
30-34	40.73747759684823
35-39	40.8669667503521
40-44	40.92453479690326
45-49	41.0360908476228
50-54	41.01028392701186
55-59	41.06505302065829
60-64	41.07823267921975
65-69	41.119285201017256
70-74	41.124549913136235
75-79	41.11578270872014
80-84	41.08735278128578
85-89	41.115825939110124
90-94	41.15722341822705
95-99	41.17527681890442
100-101	41.27349465398476
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	353.0
1	350.5
2	428.0
3	587.5
4	728.0
5	854.0
6	1099.5
7	1490.5
8	2009.5
9	2666.5
10	3535.5
11	4861.5
12	6832.0
13	9184.0
14	11706.5
15	14916.0
16	18913.5
17	24119.5
18	31238.0
19	41093.5
20	54368.0
21	71412.0
22	92483.0
23	117633.5
24	146649.0
25	179994.0
26	217334.5
27	256789.0
28	297494.5
29	337175.0
30	376635.0
31	415955.0
32	452727.0
33	488353.0
34	522316.0
35	552538.0
36	579758.0
37	601175.5
38	611754.5
39	610514.0
40	596974.0
41	572927.0
42	543106.0
43	509318.0
44	475891.5
45	448502.5
46	427037.5
47	409785.0
48	393744.0
49	375493.5
50	355758.0
51	332729.0
52	307166.0
53	283496.0
54	262390.0
55	243119.5
56	224758.5
57	206004.0
58	185899.0
59	164428.5
60	141051.5
61	118782.5
62	99194.0
63	82041.5
64	68196.5
65	56487.0
66	46318.0
67	37902.5
68	30703.0
69	24555.5
70	19451.5
71	15447.5
72	12311.5
73	9761.0
74	7937.5
75	6596.5
76	5463.0
77	4570.0
78	3742.0
79	3103.0
80	2552.5
81	1967.5
82	1496.0
83	1122.0
84	836.0
85	585.5
86	374.5
87	258.0
88	191.0
89	142.0
90	117.5
91	105.5
92	82.0
93	65.5
94	57.5
95	48.5
96	34.0
97	24.5
98	17.0
99	15.0
100	13.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07368096352190294
2	0.08040124323211559
3	0.08654568592723698
4	0.06309341476715703
5	0.05883352957597064
6	0.00599393983890436
7	0.012288885390242784
8	0.011621437940932471
9	0.020298254781966514
10-14	0.020991876641053696
15-19	0.02399931632441663
20-24	0.01913349354689558
25-29	0.0034079081529491163
30-34	0.005038573882048425
35-39	0.00907466786964254
40-44	0.023717941419315224
45-49	0.02110704404407195
50-54	0.023174822416445067
55-59	0.02642437539024409
60-64	0.021979960610130727
65-69	0.02503713167206972
70-74	0.02868977055643262
75-79	0.03026808746597818
80-84	0.026286959738915497
85-89	0.021333452688641914
90-94	0.025051527597447
95-99	0.03746081527266341
100-101	0.033477070104623044
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.5282102E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	6.528675121639936
#Duplication Level	Relative count
1	100.0
2	3.370205231044929
3	0.6746810598626104
4	0.47574348252762727
5	0.3994751888040278
6	0.2938729359559671
7	0.24800529077953662
8	0.18933737253061397
9	0.14240303793147588
10++	0.8752186713316551
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCCAG	2644020	3.5061433	3.801026	25-29
CTGGG	2587155	3.4092557	3.5908902	85-89
GGGGG	1762150	3.3364894	4.2716465	95-97
AAAAA	11047820	3.2209892	4.2617817	1
GGAGG	2440520	3.1785016	3.4445865	70-74
TTTTT	10461350	3.1386595	3.738675	10-14
CCTCC	2330475	3.1268454	3.4635942	3
CCAGG	2356120	3.1056612	3.3357341	25-29
CCTGG	2277615	3.019435	3.2472682	3
>>END_MODULE