##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename ZL4_GCCAAT_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15282102 Filtered Sequences 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.22265523420796 34.0 33.0 34.0 31.0 34.0 2 33.368712236052346 34.0 34.0 34.0 31.0 34.0 3 33.39526107076108 34.0 34.0 34.0 31.0 34.0 4 36.642443755446735 37.0 37.0 37.0 35.0 37.0 5 36.58582157088076 37.0 37.0 37.0 35.0 37.0 6 36.575236443258916 37.0 37.0 37.0 35.0 37.0 7 36.57802231656352 37.0 37.0 37.0 35.0 37.0 8 36.57468507931697 37.0 37.0 37.0 35.0 37.0 9 38.435507693902316 39.0 39.0 39.0 37.0 39.0 10-14 38.73912794195458 39.4 39.2 39.4 37.2 39.4 15-19 40.018351807886106 41.0 40.0 41.0 38.0 41.0 20-24 39.959258850647636 41.0 40.0 41.0 38.0 41.0 25-29 39.796975507688664 41.0 40.0 41.0 38.0 41.0 30-34 39.63867695687413 41.0 40.0 41.0 37.6 41.0 35-39 39.4383459814625 41.0 40.0 41.0 37.0 41.0 40-44 39.245533317340765 41.0 39.0 41.0 36.2 41.0 45-49 39.09405819958537 40.2 39.0 41.0 35.8 41.0 50-54 39.048314832606145 40.6 39.0 41.0 35.2 41.0 55-59 38.740996441458115 40.0 38.4 41.0 35.0 41.0 60-64 38.267964315380176 40.0 37.4 41.0 34.4 41.0 65-69 37.60870631540085 39.2 36.2 41.0 34.0 41.0 70-74 37.1335078119489 38.4 35.2 40.4 34.0 41.0 75-79 36.009571693736895 36.4 34.8 39.2 32.4 40.2 80-84 35.63027902836926 35.8 35.0 37.8 33.0 39.4 85-89 34.87960725559874 35.0 35.0 36.6 33.0 38.0 90-94 34.33448678722338 35.0 35.0 35.8 32.4 36.8 95-99 34.00955347634769 35.0 34.8 35.0 32.0 36.0 100-101 33.6739834938937 35.0 34.0 35.0 31.5 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 5.0 10 4.0 11 7.0 12 23.0 13 107.0 14 459.0 15 1491.0 16 3527.0 17 6645.0 18 9823.0 19 12434.0 20 14874.0 21 17034.0 22 19868.0 23 23640.0 24 28811.0 25 34874.0 26 41718.0 27 50739.0 28 62131.0 29 77012.0 30 95745.0 31 120281.0 32 156038.0 33 212786.0 34 325716.0 35 561394.0 36 1175901.0 37 2957848.0 38 6038291.0 39 3187006.0 40 45869.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.042950652842386 31.196872470896665 29.94461623588806 19.815560640372894 2 20.03831315071543 30.399512630336144 30.568068504520795 18.994105714427633 3 20.138980183352075 29.524719915857787 30.40978888081317 19.92651101997697 4 20.18454204351641 29.36086279730118 30.483668832097315 19.970926327085103 5 20.468512773962637 29.68099545468287 30.314854592647006 19.535637178707486 6 20.422367289526008 29.825072493299675 29.665173023972752 20.087387193201565 7 20.451767695307886 30.090644598498294 29.914386123060822 19.543201583133 8 19.98365800725581 30.01881547446811 30.038596784656978 19.958929733619104 9 19.963320490859175 29.797700604275512 30.312446546947534 19.92653235791778 10-14 19.973785793798932 29.921386304132092 30.14103419351773 19.96379370855125 15-19 19.991633592388173 29.759776995609 30.187999178226338 20.060590233776495 20-24 20.124847964442903 29.635784087292933 30.02341807276858 20.215949875495582 25-29 20.290992009743047 29.62556596366346 29.81951884011565 20.263923186477843 30-34 20.41981849188091 29.581066920600957 29.72225127425122 20.276863313266908 35-39 20.480995775221448 29.514437360113178 29.652842749215086 20.35172411545029 40-44 20.52504557568202 29.48836296381006 29.60802923713513 20.378562223372786 45-49 20.53749787635388 29.435637296611382 29.575930811537436 20.450934015497296 50-54 20.51845541484822 29.45454472621868 29.576917905034016 20.450081953899087 55-59 20.565172368392084 29.426135624476597 29.544964787029727 20.463727220101596 60-64 20.543706362314065 29.429386796245854 29.518858902178607 20.50804793926147 65-69 20.5534205837539 29.444934767847496 29.463984132254094 20.537660516144506 70-74 20.612383403941287 29.45126585144868 29.437274717081696 20.499076027528332 75-79 20.57496192416746 29.42583326532227 29.469929304054737 20.529275506455534 80-84 20.593713219786288 29.44303542011879 29.462932480999765 20.500318879095154 85-89 20.55476858246349 29.44389961691917 29.43685776205795 20.564474038559393 90-94 20.554985880583267 29.404110089302986 29.439555885638352 20.60134814447539 95-99 20.568471302461845 29.4349454256058 29.38736514771148 20.60921812422087 100-101 20.63033990570366 29.42513901794927 29.33201268858098 20.612508387766088 >>END_MODULE >>Per base GC content pass #Base %GC 1 38.85851129321527 2 39.032418865143065 3 40.065491203329046 4 40.15546837060151 5 40.004149952670126 6 40.50975448272757 7 39.994969278440884 8 39.94258774087491 9 39.889852848776954 10-14 39.93757950235018 15-19 40.05222382616466 20-24 40.34079783993849 25-29 40.55491519622089 30-34 40.69668180514782 35-39 40.83271989067173 40-44 40.90360779905481 45-49 40.98843189185117 50-54 40.968537368747306 55-59 41.02889958849368 60-64 41.051754301575535 65-69 41.09108109989841 70-74 41.11145943146962 75-79 41.104237430622995 80-84 41.09403209888144 85-89 41.119242621022885 90-94 41.15633402505866 95-99 41.17768942668272 100-101 41.24284829346975 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 174.0 1 179.5 2 250.0 3 416.0 4 563.0 5 650.0 6 856.0 7 1236.5 8 1727.5 9 2294.0 10 3103.0 11 4409.5 12 6296.5 13 8620.0 14 11144.0 15 14124.5 16 17951.0 17 23096.0 18 30345.5 19 40090.0 20 53552.0 21 71028.0 22 91936.5 23 116768.5 24 146155.5 25 180209.5 26 217271.0 27 255962.5 28 296700.5 29 338036.5 30 377155.5 31 414740.0 32 452402.0 33 488927.5 34 522042.0 35 552352.5 36 580447.5 37 601922.5 38 611929.0 39 610592.5 40 599029.0 41 575767.0 42 543998.5 43 509792.0 44 477095.0 45 449813.0 46 427354.0 47 409706.0 48 394855.0 49 377758.0 50 357163.0 51 333471.0 52 308135.5 53 284123.5 54 262715.5 55 242883.0 56 224676.5 57 206594.0 58 186474.5 59 164469.0 60 141496.5 61 118875.0 62 98955.5 63 82404.5 64 68331.5 65 56175.0 66 46053.5 67 37495.0 68 30249.5 69 24309.5 70 19210.5 71 15059.5 72 11924.0 73 9604.0 74 7768.5 75 6353.5 76 5241.0 77 4309.5 78 3676.0 79 2951.5 80 2268.5 81 1781.0 82 1333.5 83 969.5 84 657.0 85 410.0 86 238.5 87 150.0 88 103.5 89 73.0 90 54.0 91 39.5 92 40.0 93 31.5 94 19.5 95 16.0 96 12.0 97 8.0 98 4.5 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.9261614665312405E-5 2 0.011183016577169816 3 0.0023229788676976506 4 0.0060397450560138915 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 4.318777613184364E-5 15-19 2.3033480603649943E-4 20-24 0.0020612347699289013 25-29 0.01172875302101766 30-34 0.007123365620776514 35-39 0.030507583315436577 40-44 0.02472696491621375 45-49 0.027043400181467184 50-54 0.027773666214241995 55-59 0.02419562439774319 60-64 0.05425562530599522 65-69 0.09423441879919399 70-74 0.015874779529674646 75-79 0.01783131666049605 80-84 0.002931533895009993 85-89 0.013329318178873562 90-94 0.0159271283492284 95-99 0.009167587024350447 100-101 0.0011287714216277316 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5282102E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 6.420242199857298 #Duplication Level Relative count 1 100.0 2 3.3175860855470125 3 0.6462414554296013 4 0.4825482814882749 5 0.39083744787945357 6 0.3076578546063366 7 0.22447826133321958 8 0.19248611007432842 9 0.1604939588154373 10++ 0.918174741130176 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position CCCAG 2657060 3.528191 3.8422525 25-29 CTGGG 2597780 3.409719 3.6074512 85-89 GGAGG 2398260 3.1513858 3.4237268 70-74 CCAGG 2371035 3.1374264 3.4167464 25-29 CCTCC 2347155 3.1131742 3.4508371 70-74 TTTTT 10380310 3.038698 3.4699495 15-19 AAAAA 10057760 3.0130036 3.4634898 1 CCTGG 2286205 3.011248 3.1727424 70-74 >>END_MODULE