##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL3_ACAGTG_L008_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	11041035	
Filtered Sequences	0	
Sequence length	101	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07457154152668	34.0	31.0	34.0	30.0	34.0
2	31.962635024705563	34.0	31.0	34.0	30.0	34.0
3	31.87565694701629	34.0	31.0	34.0	30.0	34.0
4	35.0518044730408	37.0	37.0	37.0	33.0	37.0
5	35.08851715441532	37.0	37.0	37.0	33.0	37.0
6	35.43921878700684	37.0	37.0	37.0	33.0	37.0
7	34.90125672095053	37.0	37.0	37.0	33.0	37.0
8	35.26964338035338	37.0	37.0	37.0	33.0	37.0
9	36.649600603566604	39.0	38.0	39.0	33.0	39.0
10-14	36.91781870087361	39.4	38.2	39.4	33.0	39.4
15-19	37.99725947793844	41.0	39.0	41.0	33.0	41.0
20-24	38.12446193676588	41.0	39.0	41.0	32.8	41.0
25-29	37.762409393684564	41.0	39.0	41.0	32.4	41.0
30-34	37.276096670284986	40.0	38.2	41.0	30.6	41.0
35-39	37.09585644824058	40.0	38.0	41.0	30.0	41.0
40-44	37.15549040465862	40.0	38.0	41.0	30.0	41.0
45-49	36.8212316146086	40.0	38.0	41.0	29.2	41.0
50-54	36.17002145179324	39.4	37.2	40.4	28.2	40.6
55-59	36.91370374244806	40.0	37.4	41.0	29.0	41.0
60-64	36.918163940246544	40.0	36.4	41.0	29.2	41.0
65-69	36.21379745648845	39.0	35.2	41.0	27.6	41.0
70-74	35.21230471599809	37.4	35.0	40.2	26.0	41.0
75-79	34.28749444232357	36.2	34.8	39.0	25.8	40.8
80-84	33.399863943914674	35.2	34.0	37.2	24.8	39.2
85-89	32.67037508711819	35.0	34.0	36.2	24.0	37.4
90-94	32.158886481203986	35.0	34.0	35.2	23.2	36.4
95-99	31.762716412003044	35.0	33.0	35.0	21.4	36.0
100-101	31.08783039814655	34.5	32.0	35.0	19.0	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	17.0
4	104.0
5	190.0
6	1222.0
7	10454.0
8	47738.0
9	98318.0
10	113743.0
11	99593.0
12	80234.0
13	62346.0
14	47547.0
15	42382.0
16	38520.0
17	36031.0
18	34928.0
19	33531.0
20	31600.0
21	30196.0
22	30492.0
23	30486.0
24	32153.0
25	35812.0
26	40585.0
27	47603.0
28	56394.0
29	66050.0
30	80523.0
31	101551.0
32	133009.0
33	183408.0
34	275942.0
35	454660.0
36	897538.0
37	2119733.0
38	3897091.0
39	1740499.0
40	8812.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	22.416883057777287	30.254423725737254	28.498886056458545	18.829807160026917
2	21.035310425521907	34.49126940825199	26.65367457707195	17.81974558915416
3	20.229620529791298	29.643792096636297	31.40508222445296	18.721505149119448
4	20.096085295689743	28.272954112444854	28.119417809485892	23.511542782379514
5	24.561670346587544	28.413355107998783	28.146758368741942	18.87821617667173
6	25.248399663243408	28.416818186595478	27.124978662780602	19.209803487380505
7	22.609794544608334	30.93226242244492	27.823899726565536	18.634043306381205
8	19.743231082873606	33.460042074756494	27.826261719410827	18.97046512295907
9	23.897001853169193	28.868026261537	28.287445118552988	18.947526766740825
10-14	22.45048042345901	29.276792027021415	28.537926605396848	19.734800944122732
15-19	22.61046826461595	27.933752523489762	29.242522947667954	20.213256264226338
20-24	23.82568584631666	28.802844940863594	27.929941476039538	19.441527736780213
25-29	21.846576367073485	31.005944434170797	27.615044880717114	19.532434318038604
30-34	23.649113178183462	28.705644099310533	28.014805224854296	19.63043749765171
35-39	20.852216417229734	29.09809306041968	29.19688713429702	20.852803388053566
40-44	24.54905193492558	27.72272339189124	27.79398710788264	19.934237565300535
45-49	23.0756868232865	27.624922085010024	27.683599532841637	21.61579155886184
50-54	22.29740272869625	28.14027240573177	28.500162969688077	21.062161895883904
55-59	20.322483531724252	29.453545308636482	29.75126986516427	20.472701294474998
60-64	20.004363810070043	32.41255986581556	27.527018650359647	20.056057673754754
65-69	20.090849446638497	32.27276780263083	27.481477442858566	20.154905307872106
70-74	20.338867668432314	31.73132984310915	27.565759341377298	20.364043147081237
75-79	20.62413791203709	31.29808265257924	27.604275854809963	20.47350358057371
80-84	20.957020152127896	31.05256997021717	27.526306877716973	20.464102999937964
85-89	21.347621026529183	30.81913284109639	27.35485805982753	20.478388072546895
90-94	21.821251284322642	30.72823936774764	27.072857101045827	20.377652246883894
95-99	22.490009216351705	30.73066274467814	26.642765629613123	20.136562409357033
100-101	23.12954348103077	30.80699672290287	26.19749707424698	19.865962721819383
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.24669021780421
2	38.855056014676066
3	38.95112567891074
4	43.607628078069254
5	43.43988652325928
6	44.458203150623916
7	41.24383785098954
8	38.713696205832676
9	42.844528619910015
10-14	42.18528136758174
15-19	42.823724528842284
20-24	43.267213583096876
25-29	41.379010685112085
30-34	43.27955067583517
35-39	41.7050198052833
40-44	44.483289500226114
45-49	44.69147838214834
50-54	43.35956462458015
55-59	40.795184826199254
60-64	40.06042148382479
65-69	40.24575475451061
70-74	40.702910815513555
75-79	41.0976414926108
80-84	41.42112315206586
85-89	41.826009099076074
90-94	42.19890353120654
95-99	42.626571625708735
100-101	42.99550620285015
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	282.0
1	290.5
2	323.5
3	400.5
4	460.5
5	530.0
6	641.5
7	761.5
8	884.5
9	1040.5
10	1294.0
11	1614.5
12	1999.0
13	2577.5
14	3299.5
15	4435.5
16	6196.5
17	8714.5
18	12495.0
19	18066.5
20	25610.5
21	35479.5
22	48578.5
23	64941.0
24	84117.0
25	106408.5
26	131678.5
27	158956.5
28	187357.0
29	216527.5
30	245791.0
31	273024.0
32	298916.5
33	323161.0
34	344102.0
35	363204.0
36	379349.5
37	391666.0
38	400392.0
39	403859.0
40	401764.0
41	396694.5
42	389608.0
43	379712.5
44	368946.5
45	358904.0
46	349906.5
47	340755.5
48	330070.5
49	316938.5
50	300254.5
51	281901.5
52	263286.0
53	244861.5
54	227785.0
55	211964.5
56	197143.0
57	181133.0
58	162946.0
59	142869.5
60	121114.5
61	100753.0
62	82848.0
63	67114.0
64	54062.5
65	43498.0
66	35177.5
67	27999.5
68	21927.0
69	17393.5
70	13658.5
71	10715.0
72	8596.5
73	7006.5
74	5684.0
75	4672.0
76	3984.5
77	3438.0
78	2918.0
79	2454.0
80	2077.5
81	1727.0
82	1339.5
83	1030.0
84	809.5
85	578.0
86	404.5
87	290.0
88	217.5
89	169.5
90	138.0
91	113.5
92	97.0
93	72.0
94	52.5
95	48.0
96	37.0
97	29.5
98	25.5
99	20.5
100	10.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09829694408178219
2	0.08327117883423067
3	0.09341515537266207
4	0.09436615317314001
5	0.07827164754028947
6	0.03607451656479669
7	0.013232455109507397
8	0.04495955315783348
9	0.05330116243631145
10-14	0.05000074721255752
15-19	0.021315030701378992
20-24	0.01595502595544711
25-29	0.0083887063124064
30-34	0.009913925641934836
35-39	0.011810486969745137
40-44	0.026077265401296165
45-49	0.02465892011029763
50-54	0.03634804164645796
55-59	0.03976801087941484
60-64	0.026939503407062835
65-69	0.028703830754997155
70-74	0.029982696368592257
75-79	0.04563159160350456
80-84	0.04842661942471879
85-89	0.033462442606150604
90-94	0.04584715110494623
95-99	0.03543870660676286
100-101	0.05677909724948793
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.1041035E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	7.241240474995153
#Duplication Level	Relative count
1	100.0
2	5.2156066297632
3	0.5106825355146748
4	0.23792554645174774
5	0.20362997218843273
6	0.15593768922851034
7	0.10878127461645223
8	0.0985997760070306
9	0.09002588244120184
10++	0.5524802666480899
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	14088	0.1275967334584122	Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGGGGGCGCCG	11362	0.10290701913362289	Illumina Single End PCR Primer 1 (97% over 41bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	4473355	8.312492	49.855194	40-44
AAAAA	14360830	5.530773	19.476723	60-64
TTTTT	6860210	3.7638187	4.634643	10-14
CCTCC	1776945	3.5837615	4.188521	70-74
CCCAG	2047125	3.545605	4.0361514	25-29
CTCCC	1587785	3.2022617	3.659502	70-74
CTGGG	2012450	3.1786582	3.4054868	70-74
GGGAG	2249945	3.0519083	23.983824	5
CCTGG	1760445	3.0166388	3.2783172	70-74
GGAGG	2180115	2.9571884	5.457682	6
GGAAG	2672930	2.6466033	27.317019	5
GGGGC	1302495	2.6257665	10.242613	40-44
GGGGA	1928855	2.61637	11.258057	20-24
AGGGG	1852115	2.5122774	9.167907	25-29
GAGGG	1737410	2.356687	15.6213875	9
GGGAA	2141135	2.1200461	11.316214	20-24
AAGAG	2917995	2.1090531	19.684778	7
GAAGA	2754245	1.9906988	20.068624	6
GGAGA	2002850	1.9831232	18.309193	6
AGAGC	1833120	1.9691298	34.088276	8
AGAGG	1921410	1.9024855	11.266047	8
GGGCC	852590	1.864672	6.2340603	45-49
AGGGA	1827135	1.809139	5.0117297	20-24
GGAAA	2459225	1.7774656	6.0190883	20-24
GAGAG	1753080	1.7358134	17.468744	7
AGATC	1976360	1.6633673	6.5515356	95-97
CGGGG	818755	1.6505704	9.218188	35-39
GATCG	1394120	1.6073875	41.966675	1
ATCGG	1305070	1.504715	41.883865	2
GGGCG	706990	1.4252576	6.0121393	40-44
GGCGG	698150	1.4074366	7.224037	10-14
GAGCG	925830	1.3624263	45.387794	9
TCGGA	1108610	1.2782011	28.807758	3
CGGAA	1122095	1.2053497	28.00831	4
GCGTC	674215	1.1553121	7.465699	10-14
TAGGG	1085340	1.1534642	6.473061	15-19
GCGGG	568800	1.1466732	6.879048	10-14
GTAGG	1022655	1.0868447	6.4594574	15-19
CGTCG	556485	0.95357394	7.3493333	10-14
TCGGG	592840	0.93638885	20.291002	3
GCCGG	419380	0.9172123	6.942408	45-49
CTCGG	515330	0.883052	6.249949	35-39
AGCGT	744865	0.85881174	5.0406475	10-14
GGCGC	391800	0.85689306	5.222759	40-44
GCGCC	354950	0.8421928	5.611309	45-49
CGTGT	677190	0.83804625	5.730671	15-19
CGGGA	471495	0.69383925	22.094296	4
CGCCG	238090	0.5649181	6.823974	45-49
>>END_MODULE