FastQCFastQC Report
Thu 31 Jul 2014
ZL3_ACAGTG_L008_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename ZL3_ACAGTG_L008_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11041035
Filtered Sequences 0
Sequence length 101
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC 552728 5.006124878691173 TruSeq Adapter, Index 5 (100% over 50bp)

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 13876020 5.439093 23.975899 65-69
CACAC 3090240 3.5596724 15.284698 30-34
CTGGG 1995330 3.4500887 3.7105205 1
TTTTT 6642160 3.387125 3.9356759 15-19
CCCAG 2054990 3.3841074 3.957748 25-29
GGAAG 2730240 3.126887 69.11971 5
CCTCC 1785775 3.1056228 3.6086152 70-74
CCTGG 1765340 3.058295 3.3031144 65-69
GGAGG 1863195 3.0506072 3.506959 4
CCAGG 1830205 3.0081441 3.4754035 25-29
CTCCA 2192910 2.6625063 16.135422 20-24
CAGTG 2181365 2.6383188 15.994941 35-39
TCCAG 2149285 2.6045244 15.812702 25-29
CTGCT 1981735 2.5312326 16.859957 55-59
CACAG 2174495 2.500004 15.499112 30-34
TCTGC 1898690 2.4251604 16.743626 55-59
AGAGC 2105760 2.416327 68.3071 8
TTCTG 2484275 2.3349957 12.657498 55-59
GAGCA 2019455 2.3172932 68.17861 9
GCACA 1999720 2.2990663 14.685558 10-14
AGCAC 1984435 2.2814932 14.575521 10-14
GAAGA 2837885 2.2734716 48.361984 6
AAGAG 2776845 2.2245717 48.282364 7
CTTCT 2345640 2.2089367 12.8106575 50-54
ACACA 2694875 2.1672266 11.026716 30-34
GTCTG 1689175 2.153405 16.17256 15-19
ACTCC 1765740 2.143861 15.736228 20-24
CGTCT 1668700 2.1313987 15.322741 15-19
TCTTC 2156605 2.0309186 12.722144 50-54
CCAGT 1660310 2.0119796 15.380411 25-29
TGCTT 2086085 1.960733 12.696609 55-59
GATCG 1617810 1.9567099 71.599205 1
TCTGA 2156275 1.9228195 11.906881 15-19
CTGAA 2240410 1.8954389 11.298687 15-19
GCTTG 1485680 1.8939837 16.319477 55-59
GAAAA 3377805 1.8928349 8.109623 60-64
ATGCC 1560570 1.8911138 15.444479 45-49
ATCGG 1497890 1.8116688 71.51031 2
AGTGA 2113105 1.7843002 11.084857 35-39
CAGTC 1464120 1.7742345 15.18589 25-29
TCACA 2080330 1.7633967 11.035793 30-34
TCGGA 1450575 1.7544426 71.46219 3
ATCTC 1900830 1.6982949 11.302486 40-44
CTTGA 1901010 1.6951915 11.562041 60-64
TGAAA 2830890 1.6720654 8.21467 60-64
CGGAA 1446215 1.6595093 67.769104 4
GTCTT 1748020 1.6429822 12.152958 50-54
GTCAC 1353410 1.6400753 15.258293 25-29
AGATC 1818985 1.5389036 6.46109 95-97
GTGAT 1718850 1.5298078 11.333573 35-39
AACTC 1803645 1.5288639 10.894396 20-24
TGAAC 1800825 1.5235398 10.738726 20-24
ACAGT 1775385 1.502017 10.959023 30-34
TTGAA 2402865 1.4959314 8.444279 60-64
GAACT 1738400 1.4707268 10.797476 20-24
AGTCA 1692740 1.4320974 10.978812 25-29
CCGTC 815635 1.4157361 20.354841 50-54
TGATC 1538465 1.3718985 11.194419 35-39
CACGT 1128545 1.3675817 14.732805 10-14
TGCCG 788860 1.36663 21.08041 45-49
GATCT 1514225 1.3502829 11.164403 35-39
ACACG 1136975 1.3071735 13.970601 10-14
GCCGT 751825 1.3024701 20.735435 45-49
TCTCG 924865 1.1813124 15.162213 40-44
ACGTC 972475 1.1784545 14.328633 15-19
TATGC 1313540 1.1713256 11.091868 45-49
GTATG 1301800 1.1586257 10.921036 45-49
CTCGT 794910 1.0153235 15.0352335 40-44
CGTAT 769475 0.6861655 10.709678 40-44
TCGTA 730705 0.65159315 10.769096 40-44
Produced by FastQC (version 0.10.1)