##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL3_ACAGTG_L008_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	11041035	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.12000976357742	34.0	33.0	34.0	31.0	34.0
2	33.24895338163496	34.0	34.0	34.0	31.0	34.0
3	33.279313307131076	34.0	34.0	34.0	31.0	34.0
4	36.55601182316694	37.0	37.0	37.0	35.0	37.0
5	36.497731055104886	37.0	37.0	37.0	35.0	37.0
6	36.48023613728242	37.0	37.0	37.0	35.0	37.0
7	36.44922029501763	37.0	37.0	37.0	35.0	37.0
8	36.47249827575042	37.0	37.0	37.0	35.0	37.0
9	38.31692798727656	39.0	39.0	39.0	37.0	39.0
10-14	38.59820580226401	39.4	39.2	39.4	37.2	39.4
15-19	39.84623581032032	41.0	40.0	41.0	38.0	41.0
20-24	39.773483699671274	41.0	40.0	41.0	37.8	41.0
25-29	39.60403089021998	41.0	40.0	41.0	37.0	41.0
30-34	39.37908893505002	41.0	39.2	41.0	36.8	41.0
35-39	39.104257580924255	40.6	38.8	41.0	35.6	41.0
40-44	38.81874882200809	40.0	38.2	41.0	35.0	41.0
45-49	38.78378809595296	40.0	38.4	41.0	35.0	41.0
50-54	38.771488361371915	40.0	38.6	41.0	35.0	41.0
55-59	38.47237662048893	40.0	38.0	41.0	34.6	41.0
60-64	37.93720739043033	39.6	36.8	41.0	33.8	41.0
65-69	37.12360717994282	38.8	35.6	40.6	32.8	41.0
70-74	35.93768082430678	37.4	35.0	39.8	30.0	41.0
75-79	34.39283389645989	36.0	34.4	39.0	28.2	40.0
80-84	33.9631868751435	35.4	35.0	37.2	29.0	39.2
85-89	33.216307764625334	35.0	34.0	36.2	27.4	37.6
90-94	32.70566632566603	35.0	34.0	35.4	26.8	36.4
95-99	32.39754289339722	35.0	34.0	35.0	25.8	36.0
100-101	32.04476097575997	35.0	33.5	35.0	24.5	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	8.0
10	43.0
11	82.0
12	134.0
13	328.0
14	667.0
15	1511.0
16	3142.0
17	5486.0
18	8138.0
19	10801.0
20	13273.0
21	16932.0
22	21195.0
23	27219.0
24	35053.0
25	44882.0
26	59472.0
27	80757.0
28	120893.0
29	217584.0
30	298155.0
31	156878.0
32	131529.0
33	174973.0
34	264092.0
35	442226.0
36	898610.0
37	2171783.0
38	4009289.0
39	1815248.0
40	10649.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.459730406288987	29.075004062219616	28.20313489231993	19.262130639171463
2	19.83451259990736	33.76410876413141	28.20309048213311	18.19828815382812
3	19.911430404848822	27.556891179133114	33.540071198035335	18.991607217982732
4	19.94889971818765	27.441095875522542	28.11302563573071	24.4969787705591
5	25.756625171462638	27.838612956122322	27.92071576623025	18.484046106184792
6	25.582203117733076	28.025026639259814	27.40481304515383	18.98795719785328
7	20.124526369131154	33.538594887164116	27.721893826076993	18.61498491762774
8	19.709139587004298	33.4702226738707	27.745904256258587	19.074733482866417
9	24.9273822608116	27.799622046302723	28.126348662059307	19.146647030826365
10-14	20.541875742143674	30.084720310557696	28.048290385006098	21.325113562292536
15-19	20.53902774681209	28.78446541682097	29.18262340649673	21.493883429870206
20-24	20.666772514787414	29.785030799357703	28.945981863742677	20.60221482211221
25-29	20.833672265149964	28.663975526906572	28.75186107535997	21.750491132583498
30-34	19.84768596826398	29.71581575049572	28.625721756704607	21.810776524535683
35-39	22.102253657502978	28.577970496232346	28.590654163204405	20.729121683060274
40-44	19.976522389976058	28.51426104291295	29.660266547364238	21.848950019746752
45-49	22.143952129626392	28.438403460657717	29.60330849916506	19.81433591055083
50-54	20.997077802487322	27.374655783998403	28.55379008450273	23.07447632901155
55-59	21.01657796733542	27.362299107537563	30.68233865336066	20.938784271766366
60-64	20.999151328142553	28.567382358248032	29.51596565177656	20.917500661832854
65-69	19.87603368262678	32.993725596808694	27.25733972541777	19.872900995146765
70-74	20.02583205838862	32.648168857824814	27.29449188006657	20.031507203719993
75-79	20.25716026441958	31.830035263142292	27.479411836962665	20.433392635475464
80-84	20.539937330892673	31.303792096369556	27.497813983149417	20.658456589588358
85-89	20.81520256727897	31.003201851629576	27.3577454326395	20.823850148451957
90-94	21.087004795723978	30.91240159379702	27.05936041370084	20.941233196778157
95-99	21.4191210044142	30.980717472516524	26.59615676332379	21.004004759745488
100-101	21.698312853964435	31.119112382402076	26.13345267310074	21.049122090532745
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.72186104546045
2	38.03280075373548
3	38.903037622831555
4	44.44587848874676
5	44.24067127764743
6	44.57016031558636
7	38.73951128675889
8	38.783873069870715
9	44.07402929163797
10-14	41.86698930443622
15-19	42.03291117668229
20-24	41.26898733689963
25-29	42.58416339773346
30-34	41.658462492799664
35-39	42.831375340563255
40-44	41.82547240972281
45-49	41.95828804017722
50-54	44.07155413149887
55-59	41.95536223910178
60-64	41.916651989975406
65-69	39.74893467777354
70-74	40.05733926210861
75-79	40.690552899895046
80-84	41.19839392048103
85-89	41.63905271573093
90-94	42.028237992502135
95-99	42.423125764159686
100-101	42.74743494449718
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	148.0
1	143.5
2	161.0
3	222.0
4	281.5
5	332.5
6	387.5
7	461.5
8	549.5
9	643.5
10	837.5
11	1119.5
12	1430.5
13	1903.0
14	2626.0
15	3741.5
16	5418.5
17	7926.5
18	11700.0
19	17114.0
20	24524.0
21	34438.0
22	47430.5
23	63531.0
24	82833.0
25	105292.0
26	130315.5
27	157529.5
28	186435.0
29	215692.5
30	244170.0
31	271642.5
32	297645.0
33	323802.0
34	349962.0
35	373797.0
36	396587.5
37	418402.5
38	435479.5
39	445153.5
40	445837.0
41	437248.5
42	422019.5
43	402076.0
44	380320.0
45	359275.0
46	340301.0
47	325043.5
48	311005.5
49	294836.0
50	277408.5
51	259960.5
52	242212.5
53	224903.0
54	209969.0
55	197438.5
56	185406.0
57	171163.0
58	154261.0
59	135258.0
60	115105.5
61	95951.5
62	78505.5
63	63239.0
64	50708.5
65	40953.5
66	32918.0
67	25944.5
68	20433.0
69	16055.5
70	12330.5
71	9699.5
72	7790.0
73	6253.5
74	5079.0
75	4149.0
76	3411.0
77	2936.0
78	2596.0
79	2163.5
80	1809.5
81	1480.5
82	1158.5
83	913.0
84	636.5
85	402.0
86	252.5
87	155.5
88	105.5
89	64.0
90	40.0
91	26.0
92	21.5
93	21.5
94	15.0
95	11.5
96	7.5
97	4.5
98	4.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0024725942812426553
2	0.005044816903487762
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	4.1843903221029546E-4
15-19	0.001280677037976965
20-24	5.253130707401978E-4
25-29	0.013131015344123082
30-34	0.008357912097914734
35-39	0.029142195455407936
40-44	0.01586083188759025
45-49	0.018327991895687318
50-54	0.025678752037286357
55-59	0.018967424702484866
60-64	0.03858696218244032
65-69	0.08127680058979977
70-74	0.03108585381714667
75-79	0.017873324375839765
80-84	0.005753083836796098
85-89	0.019110527228652023
90-94	0.03164558395114226
95-99	0.014683406039379461
100-101	0.0026220367927463325
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.1041035E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	11.26557892295386
#Duplication Level	Relative count
1	100.0
2	5.212228546433163
3	0.4272843174386412
4	0.18083568490848861
5	0.15523063217808314
6	0.12215743906797608
7	0.10135333372452164
8	0.07948235118396697
9	0.06027856163616287
10++	0.3488688434517745
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC	552728	5.006124878691173	TruSeq Adapter, Index 5 (100% over 50bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	13876020	5.439093	23.975899	65-69
CACAC	3090240	3.5596724	15.284698	30-34
CTGGG	1995330	3.4500887	3.7105205	1
TTTTT	6642160	3.387125	3.9356759	15-19
CCCAG	2054990	3.3841074	3.957748	25-29
GGAAG	2730240	3.126887	69.11971	5
CCTCC	1785775	3.1056228	3.6086152	70-74
CCTGG	1765340	3.058295	3.3031144	65-69
GGAGG	1863195	3.0506072	3.506959	4
CCAGG	1830205	3.0081441	3.4754035	25-29
CTCCA	2192910	2.6625063	16.135422	20-24
CAGTG	2181365	2.6383188	15.994941	35-39
TCCAG	2149285	2.6045244	15.812702	25-29
CTGCT	1981735	2.5312326	16.859957	55-59
CACAG	2174495	2.500004	15.499112	30-34
TCTGC	1898690	2.4251604	16.743626	55-59
AGAGC	2105760	2.416327	68.3071	8
TTCTG	2484275	2.3349957	12.657498	55-59
GAGCA	2019455	2.3172932	68.17861	9
GCACA	1999720	2.2990663	14.685558	10-14
AGCAC	1984435	2.2814932	14.575521	10-14
GAAGA	2837885	2.2734716	48.361984	6
AAGAG	2776845	2.2245717	48.282364	7
CTTCT	2345640	2.2089367	12.8106575	50-54
ACACA	2694875	2.1672266	11.026716	30-34
GTCTG	1689175	2.153405	16.17256	15-19
ACTCC	1765740	2.143861	15.736228	20-24
CGTCT	1668700	2.1313987	15.322741	15-19
TCTTC	2156605	2.0309186	12.722144	50-54
CCAGT	1660310	2.0119796	15.380411	25-29
TGCTT	2086085	1.960733	12.696609	55-59
GATCG	1617810	1.9567099	71.599205	1
TCTGA	2156275	1.9228195	11.906881	15-19
CTGAA	2240410	1.8954389	11.298687	15-19
GCTTG	1485680	1.8939837	16.319477	55-59
GAAAA	3377805	1.8928349	8.109623	60-64
ATGCC	1560570	1.8911138	15.444479	45-49
ATCGG	1497890	1.8116688	71.51031	2
AGTGA	2113105	1.7843002	11.084857	35-39
CAGTC	1464120	1.7742345	15.18589	25-29
TCACA	2080330	1.7633967	11.035793	30-34
TCGGA	1450575	1.7544426	71.46219	3
ATCTC	1900830	1.6982949	11.302486	40-44
CTTGA	1901010	1.6951915	11.562041	60-64
TGAAA	2830890	1.6720654	8.21467	60-64
CGGAA	1446215	1.6595093	67.769104	4
GTCTT	1748020	1.6429822	12.152958	50-54
GTCAC	1353410	1.6400753	15.258293	25-29
AGATC	1818985	1.5389036	6.46109	95-97
GTGAT	1718850	1.5298078	11.333573	35-39
AACTC	1803645	1.5288639	10.894396	20-24
TGAAC	1800825	1.5235398	10.738726	20-24
ACAGT	1775385	1.502017	10.959023	30-34
TTGAA	2402865	1.4959314	8.444279	60-64
GAACT	1738400	1.4707268	10.797476	20-24
AGTCA	1692740	1.4320974	10.978812	25-29
CCGTC	815635	1.4157361	20.354841	50-54
TGATC	1538465	1.3718985	11.194419	35-39
CACGT	1128545	1.3675817	14.732805	10-14
TGCCG	788860	1.36663	21.08041	45-49
GATCT	1514225	1.3502829	11.164403	35-39
ACACG	1136975	1.3071735	13.970601	10-14
GCCGT	751825	1.3024701	20.735435	45-49
TCTCG	924865	1.1813124	15.162213	40-44
ACGTC	972475	1.1784545	14.328633	15-19
TATGC	1313540	1.1713256	11.091868	45-49
GTATG	1301800	1.1586257	10.921036	45-49
CTCGT	794910	1.0153235	15.0352335	40-44
CGTAT	769475	0.6861655	10.709678	40-44
TCGTA	730705	0.65159315	10.769096	40-44
>>END_MODULE