##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL3_ACAGTG_L007_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	10869007	
Filtered Sequences	0	
Sequence length	101	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21695036170278	34.0	31.0	34.0	30.0	34.0
2	32.14544070125265	34.0	31.0	34.0	30.0	34.0
3	32.06437938626776	34.0	31.0	34.0	30.0	34.0
4	35.2483834079783	37.0	37.0	37.0	35.0	37.0
5	35.29154650466229	37.0	37.0	37.0	35.0	37.0
6	35.60403797697435	37.0	37.0	37.0	35.0	37.0
7	35.10183579787923	37.0	37.0	37.0	35.0	37.0
8	35.45418436109205	37.0	37.0	37.0	35.0	37.0
9	36.86962994871565	39.0	38.0	39.0	34.0	39.0
10-14	37.14209110363072	39.4	38.4	39.4	34.0	39.4
15-19	38.248750028406455	41.0	39.4	41.0	34.0	41.0
20-24	38.3402100670282	41.0	39.0	41.0	33.8	41.0
25-29	38.034372155616424	41.0	39.0	41.0	33.4	41.0
30-34	37.563358842256704	40.4	38.8	41.0	32.2	41.0
35-39	37.393793066836736	40.0	38.0	41.0	31.0	41.0
40-44	37.46554547255329	40.0	38.0	41.0	31.2	41.0
45-49	37.12519785846122	40.0	38.0	41.0	30.4	41.0
50-54	36.45728379786673	39.4	37.4	40.4	29.8	40.8
55-59	37.18013364054325	40.0	37.6	41.0	30.6	41.0
60-64	37.16305633072092	40.0	36.6	41.0	30.6	41.0
65-69	36.44866772097948	39.0	35.2	41.0	29.2	41.0
70-74	35.4773158578332	37.6	35.0	40.2	28.0	41.0
75-79	34.5540749766745	36.4	35.0	39.0	27.4	40.8
80-84	33.682512763125466	35.2	34.4	37.2	26.2	39.2
85-89	32.947953626306436	35.0	34.0	36.2	25.8	37.6
90-94	32.429975765035394	35.0	34.0	35.4	25.0	36.4
95-99	32.044410588750196	35.0	33.8	35.0	24.2	36.0
100-101	31.405343238807372	34.5	32.0	35.0	21.0	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	18.0
5	66.0
6	752.0
7	7614.0
8	32291.0
9	71029.0
10	93195.0
11	89824.0
12	75752.0
13	61195.0
14	46787.0
15	40578.0
16	37650.0
17	34869.0
18	33954.0
19	32386.0
20	30370.0
21	29219.0
22	28309.0
23	28782.0
24	30456.0
25	33827.0
26	38113.0
27	45410.0
28	53935.0
29	61358.0
30	74664.0
31	94387.0
32	124138.0
33	169921.0
34	258553.0
35	426886.0
36	854281.0
37	2069689.0
38	3924408.0
39	1824811.0
40	9525.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.923382879365967	30.395966221292518	28.71908283369916	18.961568065642357
2	20.8782609320166	34.338263499088725	26.82125388398565	17.96222168490902
3	20.318575019189602	29.61967571414049	31.206335367946807	18.8554138987231
4	20.2541427308882	28.293719619973484	28.18605464722345	23.26608300191486
5	24.328130264516947	28.481066610695933	28.252369090162592	18.938434034624528
6	24.796092153483634	28.592413411738075	27.299977273542815	19.31151716123547
7	22.269906665064372	30.947081739702693	28.030358100146284	18.75265349508665
8	19.819624764522022	33.06574133892368	28.02590296885595	19.088730927698357
9	23.60000500602932	28.83473808896303	28.47895111906841	19.086305785939242
10-14	22.128975913732578	29.32093454215523	28.71724935093264	19.832840193179553
15-19	22.245675972688502	28.086619985265404	29.403899227836096	20.26380481421
20-24	23.383533783978276	28.924538137523196	28.10302619113297	19.588901887365562
25-29	21.7077294742884	30.815494382596437	27.800877444663968	19.675898698451196
30-34	23.258408998648935	28.756867108139172	28.208532640638857	19.77619125257304
35-39	20.73078875874376	29.06981186484626	29.264145009430404	20.935254366979578
40-44	24.176531420646047	27.839054391247135	27.94252838614507	20.041885801961744
45-49	22.69721828923159	27.777854055199125	27.842145492044274	21.682782163525008
50-54	22.017370996080633	28.300234437716572	28.614934637950384	21.06745992825242
55-59	20.347069670287567	29.486705471608737	29.672657475929494	20.4935673821742
60-64	20.108583602501547	32.09051170960407	27.655198454785683	20.145706233108697
65-69	20.210840976342958	31.869704689524493	27.65091761393064	20.268536720201908
70-74	20.459942947039785	31.357624154278852	27.720526010159375	20.461906888521987
75-79	20.724149158266183	30.954623940008887	27.74618564989955	20.57504125182538
80-84	21.018580155295474	30.754779501309553	27.67167277682141	20.554967566573563
85-89	21.393390980987288	30.54002418239035	27.50908312019387	20.55750171642849
90-94	21.84085907431665	30.490285921898284	27.2245456956558	20.444309308129267
95-99	22.501811491360467	30.51499258063502	26.781076492316558	20.20211943568795
100-101	23.107044063481617	30.59390194108631	26.37337520147987	19.9256787939522
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	40.88495094500833
2	38.84048261692563
3	39.1739889179127
4	43.52022573280306
5	43.26656429914147
6	44.10760931471911
7	41.02256016015102
8	38.908355692220375
9	42.686310791968566
10-14	41.96181610691213
15-19	42.5094807868985
20-24	42.97243567134383
25-29	41.38362817273959
30-34	43.034600251221974
35-39	41.666043125723334
40-44	44.21841722260779
45-49	44.3800004527566
50-54	43.08483092433305
55-59	40.840637052461766
60-64	40.254289835610244
65-69	40.479377696544866
70-74	40.92184983556177
75-79	41.29919041009156
80-84	41.57354772186903
85-89	41.95089269741578
90-94	42.28516838244592
95-99	42.703930927048425
100-101	43.03272285743381
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	301.0
1	292.0
2	316.0
3	373.5
4	456.5
5	534.5
6	624.5
7	726.5
8	874.0
9	1063.0
10	1253.0
11	1546.5
12	1937.5
13	2434.0
14	3186.5
15	4361.5
16	6037.5
17	8489.5
18	12282.5
19	17574.5
20	25092.5
21	35147.5
22	47793.0
23	63598.0
24	82562.5
25	105126.0
26	130434.0
27	156890.5
28	185140.5
29	213935.5
30	241832.5
31	269132.0
32	294992.5
33	319261.0
34	341053.5
35	359598.5
36	374713.0
37	387053.5
38	395580.5
39	398050.5
40	396805.5
41	393399.0
42	385481.0
43	374306.5
44	363149.0
45	352990.5
46	343899.5
47	334233.0
48	322948.5
49	309750.5
50	294634.5
51	276759.0
52	257771.5
53	240167.5
54	223649.0
55	208668.0
56	193712.0
57	177614.0
58	159752.0
59	139567.5
60	118567.0
61	98895.0
62	81258.0
63	65969.0
64	53098.5
65	42525.5
66	34091.0
67	27104.5
68	21482.5
69	16903.5
70	13182.5
71	10370.0
72	8180.0
73	6550.0
74	5378.0
75	4391.5
76	3699.0
77	3157.0
78	2703.0
79	2331.5
80	1919.5
81	1541.5
82	1226.0
83	963.5
84	761.0
85	525.5
86	372.0
87	259.0
88	169.5
89	126.0
90	104.0
91	83.5
92	72.0
93	70.0
94	55.0
95	39.0
96	37.5
97	35.0
98	23.5
99	14.5
100	8.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07109205100337132
2	0.07714596190801976
3	0.08242703312271304
4	0.06078752180396976
5	0.05692332335419418
6	0.005676691532170326
7	0.011638597711824088
8	0.011206175504349202
9	0.019422197446372056
10-14	0.020095672033332942
15-19	0.02400771294010575
20-24	0.01925290875238189
25-29	0.00327352811530989
30-34	0.004885450897216277
35-39	0.008915257851982246
40-44	0.023080305312159613
45-49	0.020618258871302596
50-54	0.022180499101711867
55-59	0.02541354513802411
60-64	0.021346936293260278
65-69	0.024787913008060443
70-74	0.02854722607134212
75-79	0.02930166481629831
80-84	0.025569953170515025
85-89	0.020761786242294262
90-94	0.02404083464110383
95-99	0.03612289512740216
100-101	0.031902638391897255
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.0869007E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	6.950099045381706
#Duplication Level	Relative count
1	100.0
2	5.189511858532045
3	0.4674946778351894
4	0.24551493950383516
5	0.18721182750837104
6	0.15458348042834066
7	0.09948971404730575
8	0.09948971404730575
9	0.0722102763246574
10++	0.4626806594135456
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	14002	0.1288250159375185	Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGGGGGCGCCG	11351	0.10443456334143496	Illumina Single End PCR Primer 1 (97% over 41bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	3589830	6.9643245	43.276684	40-44
AAAAA	13267250	5.275164	18.307089	60-64
TTTTT	6792130	3.7009552	4.6348476	10-14
CCCAG	2039250	3.5696552	4.0629	25-29
CCTCC	1763165	3.5304186	4.0999827	70-74
CTGGG	1991055	3.2180722	3.4176378	35-39
CTCCC	1574460	3.152571	3.644278	70-74
CCTGG	1742425	3.0246391	3.2613466	65-69
GGGAG	2123605	3.0006053	21.486511	5
GGAGG	2096685	2.9625678	5.048752	6
GGAAG	2588885	2.6642678	27.08777	5
GGGGC	1209900	2.520934	10.058053	40-44
GGGGA	1771045	2.502446	9.9415045	20-24
AGGGG	1688880	2.3863487	8.188073	25-29
GAGGG	1613670	2.280079	12.68889	9
AAGAG	2831320	2.1221888	19.49365	7
GGGAA	2040430	2.099843	10.7274	20-24
GAAGA	2672740	2.003327	19.849913	6
AGAGC	1794765	1.9837165	33.51906	8
GGAGA	1916430	1.9722323	16.080143	6
AGAGG	1833325	1.8867074	9.33456	8
GGGCC	828310	1.853582	5.9925528	45-49
AGGGA	1785810	1.837809	5.115785	20-24
GGAAA	2377770	1.7822348	5.748259	20-24
GAGAG	1686105	1.7352008	15.383193	7
AGATC	1946445	1.6688399	6.6574316	95-97
GATCG	1351175	1.5905747	40.18491	1
CGGGG	739000	1.5397722	8.898511	35-39
ATCGG	1258840	1.4818801	40.11911	2
GAGCG	896985	1.3612163	44.65569	9
GGGCG	635325	1.3237561	5.9343925	40-44
GGCGG	622445	1.2969195	5.9892163	10-14
TCGGA	1079570	1.2708473	28.204916	3
CGGAA	1090815	1.2056552	27.578213	4
TAGGG	1063155	1.1652852	6.4895205	15-19
GCGTC	660920	1.1472772	7.4979033	10-14
GTAGG	1001910	1.0981567	6.473681	15-19
GCGGG	498545	1.0387628	5.6826215	10-14
CGTCG	544905	0.9458892	7.362695	10-14
GCCGG	407155	0.91112643	6.7664227	45-49
TCGGG	562145	0.90857524	18.015707	3
CTCGG	510425	0.8860361	6.2609386	35-39
AGCGT	734075	0.86413777	5.1053395	10-14
GGCGC	379100	0.8483454	5.247972	40-44
GCGCC	348255	0.8369956	5.5540757	45-49
CGTGT	665355	0.834193	5.7104206	15-19
CGGGA	445590	0.67620355	19.480185	4
CGCCG	236460	0.56830764	6.938456	45-49
>>END_MODULE