FastQCFastQC Report
Thu 31 Jul 2014
ZL3_ACAGTG_L007_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename ZL3_ACAGTG_L007_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 10869007
Filtered Sequences 0
Sequence length 101
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC 477524 4.39344642983485 TruSeq Adapter, Index 5 (100% over 50bp)

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 12626380 5.1180778 21.879183 65-69
CTGGG 1984235 3.4585724 3.7417414 1
CACAC 2909830 3.427267 13.795973 30-34
CCCAG 2034170 3.4071524 3.934768 25-29
TTTTT 6561250 3.3592737 3.9205124 15-19
CCTCC 1774460 3.1250007 3.6066837 70-74
CCTGG 1753530 3.0669758 3.296629 70-74
GGAAG 2626890 3.06226 61.69405 5
GGAGG 1840440 3.0510259 3.4609761 4
CCAGG 1812565 3.0255516 3.4297068 25-29
CTCCA 2099565 2.5911694 14.507771 20-24
CAGTG 2095515 2.5684462 14.379246 35-39
TCCAG 2050035 2.521357 14.192118 25-29
CACAG 2083020 2.4450085 14.001747 30-34
CTGCT 1890370 2.4361615 15.018838 55-59
AGAGC 2010390 2.3516562 60.94268 8
TCTGC 1810795 2.3336115 14.915581 55-59
TTCTG 2389130 2.2608292 11.289177 55-59
GAGCA 1929030 2.2564852 60.821323 9
GCACA 1903240 2.2339861 13.244396 10-14
GAAGA 2720210 2.2298646 43.38034 6
AGCAC 1889875 2.2182987 13.119656 10-14
AAGAG 2661285 2.1815617 43.28973 7
CTTCT 2250530 2.1370082 11.471727 50-54
ACACA 2578005 2.1278777 10.0407 30-34
ACTCC 1677505 2.0702858 14.102466 20-24
GTCTG 1599780 2.0545945 14.330257 15-19
TCTTC 2067135 1.962864 11.353002 50-54
CGTCT 1502890 1.9368075 13.488655 15-19
CCAGT 1565775 1.9257611 13.774099 25-29
TGCTT 1993170 1.886133 11.301661 55-59
GAAAA 3246090 1.8711672 7.450244 60-64
GATCG 1525985 1.8703805 63.31105 1
TCTGA 2057800 1.8584249 10.673142 15-19
CTGAA 2139520 1.8440472 10.176448 15-19
ATGCC 1471920 1.810328 13.78502 45-49
GCTTG 1397775 1.79516 14.429228 55-59
AGTGA 2026550 1.7406826 9.980137 35-39
ATCGG 1403345 1.7200623 63.256344 2
TCACA 1985615 1.7172917 9.949882 30-34
CAGTC 1379220 1.6963155 13.554235 25-29
TCGGA 1356995 1.6632516 63.20224 3
TGAAA 2725010 1.645911 7.4650903 60-64
ATCTC 1804695 1.6354564 10.109447 40-44
CTTGA 1809685 1.6343491 10.36236 60-64
CGGAA 1350910 1.5802288 60.331158 4
GTCTT 1661330 1.5721134 10.804132 50-54
GTCAC 1272085 1.5645491 13.614001 25-29
AGATC 1797390 1.5491658 6.5494123 95-97
AACTC 1715200 1.4834188 9.819832 20-24
TGAAC 1708965 1.4729527 9.650281 20-24
GTGAT 1632395 1.4691755 10.094617 35-39
TTGAA 2305090 1.4588555 7.6264997 60-64
ACAGT 1688205 1.4550599 9.841981 30-34
GAACT 1645840 1.4185452 9.686728 20-24
AGTCA 1608795 1.3866165 9.868408 25-29
TGATC 1455150 1.3141639 9.952772 35-39
CCGTC 732145 1.2849542 17.955198 50-54
CACGT 1044625 1.284794 13.078234 10-14
GATCT 1421365 1.2836524 9.903889 35-39
TGCCG 707405 1.2372723 18.592226 45-49
ACACG 1047685 1.2297524 12.478316 10-14
GCCGT 669885 1.1716486 18.267666 45-49
TATGC 1224285 1.1056669 9.865459 45-49
GTATG 1221120 1.099023 9.71129 45-49
ACGTC 886445 1.090247 12.69372 15-19
TCTCG 841915 1.0849944 13.330855 40-44
CTCGT 712900 0.91872996 13.204095 40-44
CGTAT 688010 0.6213504 9.456603 40-44
TCGTA 649040 0.58615613 9.5089035 40-44
Produced by FastQC (version 0.10.1)