##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL3_ACAGTG_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	10869007	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.1200583457164	34.0	33.0	34.0	31.0	34.0
2	33.26025532967271	34.0	34.0	34.0	31.0	34.0
3	33.2974279987123	34.0	34.0	34.0	31.0	34.0
4	36.57526000305272	37.0	37.0	37.0	35.0	37.0
5	36.51970773411039	37.0	37.0	37.0	35.0	37.0
6	36.50773138705311	37.0	37.0	37.0	35.0	37.0
7	36.47809427300948	37.0	37.0	37.0	35.0	37.0
8	36.48989599509873	37.0	37.0	37.0	35.0	37.0
9	38.34826769363568	39.0	39.0	39.0	37.0	39.0
10-14	38.629790504321136	39.4	39.2	39.4	37.2	39.4
15-19	39.89273833386987	41.0	40.0	41.0	38.0	41.0
20-24	39.8283288068542	41.0	40.0	41.0	38.0	41.0
25-29	39.670564072688514	41.0	40.0	41.0	37.6	41.0
30-34	39.46794705348888	41.0	39.8	41.0	37.0	41.0
35-39	39.208780949354434	41.0	39.2	41.0	36.0	41.0
40-44	38.94074343681994	40.0	39.0	41.0	35.0	41.0
45-49	38.89871293670158	40.0	38.6	41.0	35.0	41.0
50-54	38.88082616930875	40.0	38.8	41.0	35.0	41.0
55-59	38.59688626569107	40.0	38.0	41.0	34.8	41.0
60-64	38.07032763894623	39.8	37.0	41.0	33.8	41.0
65-69	37.26877250148059	39.0	35.6	40.8	33.0	41.0
70-74	36.16145272516616	37.4	35.0	40.0	30.8	41.0
75-79	34.64874338566531	36.0	34.4	39.0	29.4	40.0
80-84	34.228465360267045	35.4	35.0	37.2	30.0	39.2
85-89	33.506509288291014	35.0	34.6	36.2	29.0	37.6
90-94	32.997379227007585	35.0	34.0	35.4	29.0	36.6
95-99	32.696162896941736	35.0	34.0	35.0	28.8	36.0
100-101	32.36630406991182	35.0	34.0	35.0	26.0	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	1.0
8	5.0
9	5.0
10	22.0
11	47.0
12	83.0
13	230.0
14	602.0
15	1498.0
16	3119.0
17	5546.0
18	7934.0
19	10086.0
20	12286.0
21	15220.0
22	19544.0
23	24773.0
24	33137.0
25	42761.0
26	55144.0
27	75680.0
28	110934.0
29	194245.0
30	254821.0
31	130904.0
32	118361.0
33	157221.0
34	237317.0
35	402780.0
36	832468.0
37	2103097.0
38	4071191.0
39	1935773.0
40	12170.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	22.996143621242236	29.27765697044026	28.372716729820258	19.35348267849725
2	19.978619403857156	33.317917760341146	28.384471260875475	18.31899157492622
3	20.07322268412698	27.74817152605131	33.06811455912692	19.11049123069479
4	20.13581704980032	27.600841482000547	28.29958915922095	23.963752308978176
5	25.28008308394686	28.016487614737944	28.071055617132274	18.632373684182927
6	25.09446355127014	28.178618341123528	27.60840985749664	19.11850825010969
7	20.27693054204492	33.054491546467865	27.917922952851164	18.750654958636055
8	19.87582674295821	33.014165875502705	27.938283598492486	19.171723783046602
9	24.41291094945472	27.94767728091444	28.36420107191025	19.275210697720592
10-14	20.564733104512523	30.006681939583807	28.224179376652486	21.204405579251183
15-19	20.564780902979724	28.82814737876861	29.242791646104326	21.36428007214734
20-24	20.688082174806212	29.694715454320527	28.99648935487797	20.620713015995296
25-29	20.864945096390443	28.70663637320932	28.795469953027574	21.632948577372666
30-34	19.986335949988238	29.641292371951884	28.67007601754958	21.7022956605103
35-39	21.99221037376596	28.61704085610164	28.643605226871266	20.74714354326113
40-44	20.109305414022458	28.55774698261333	29.58083081567716	21.75211678768705
45-49	22.047523705631818	28.484965267864766	29.52346808451935	19.94404294198407
50-54	21.022826173885107	27.556912204326323	28.600822511264735	22.819439110523835
55-59	21.034449927154096	27.552688146077507	30.47465579696374	20.93820612980465
60-64	20.99854157949209	28.612947024222713	29.44741850585688	20.94109289042832
65-69	20.023384406637167	32.51762454519909	27.4409587842466	20.018032263917142
70-74	20.1678085087204	32.21720301582784	27.456070768945256	20.1589177065065
75-79	20.380117838904173	31.429709320948923	27.655910547041103	20.5342622931058
80-84	20.660133584289984	30.965846965073073	27.64699288135865	20.72702656927829
85-89	20.88388950396627	30.743577635197607	27.49823880935415	20.874294051481975
90-94	21.160488133362314	30.677218878774397	27.19797396938612	20.964319018477166
95-99	21.465826800286123	30.77983982080364	26.74343522378106	21.010898155129176
100-101	21.725601995640588	30.922354451801937	26.28208342507069	21.069960127486787
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.34962629973949
2	38.297610978783375
3	39.18371391482177
4	44.099569358778496
5	43.91245676812978
6	44.21297180137983
7	39.02758550068097
8	39.04755052600481
9	43.68812164717531
10-14	41.76913868376371
15-19	41.92906097512707
20-24	41.308795190801504
25-29	42.49789367376311
30-34	41.68863161049854
35-39	42.739353917027096
40-44	41.86142220170951
45-49	41.99156664761588
50-54	43.842265284408946
55-59	41.97265605695875
60-64	41.939634469920406
65-69	40.041416670554305
70-74	40.326726215226905
75-79	40.914380132009974
80-84	41.387160153568274
85-89	41.75818355544824
90-94	42.124807151839484
95-99	42.4767249554153
100-101	42.795562123127375
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	177.0
1	157.5
2	158.5
3	216.0
4	284.5
5	323.5
6	379.5
7	456.0
8	561.0
9	722.5
10	909.0
11	1123.0
12	1409.0
13	1873.0
14	2586.5
15	3746.0
16	5402.0
17	7755.5
18	11432.0
19	16795.5
20	24233.5
21	34043.0
22	46629.5
23	62662.0
24	81881.0
25	104149.5
26	129309.5
27	155856.5
28	183712.0
29	212190.5
30	240575.0
31	268962.5
32	294639.5
33	319028.5
34	343831.5
35	366253.5
36	387937.0
37	407843.0
38	423043.0
39	431163.5
40	432243.0
41	425909.5
42	411774.5
43	393413.5
44	373771.0
45	354635.0
46	337402.0
47	322343.5
48	307292.0
49	292190.0
50	276092.0
51	258215.0
52	239747.0
53	223552.5
54	209632.5
55	196532.5
56	183905.0
57	169822.5
58	153456.0
59	134455.0
60	114544.0
61	95246.5
62	77638.0
63	62952.5
64	50519.5
65	40591.0
66	32933.0
67	26060.0
68	20184.5
69	15865.5
70	12367.5
71	9641.5
72	7524.5
73	5996.0
74	4941.0
75	4029.0
76	3312.0
77	2868.0
78	2476.5
79	2074.5
80	1693.0
81	1348.0
82	1066.0
83	790.5
84	587.5
85	396.5
86	233.5
87	137.5
88	76.0
89	55.0
90	46.5
91	27.0
92	21.5
93	19.0
94	10.5
95	11.0
96	9.5
97	4.0
98	2.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.8400944998931365E-5
2	0.01112337125185401
3	0.0022449152898696267
4	0.0051798660171991796
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	6.624340199615291E-5
15-19	2.5393304098525287E-4
20-24	0.0021657912263742217
25-29	0.011682759979821525
30-34	0.0071745284550833385
35-39	0.03059341115522329
40-44	0.02468854790506621
45-49	0.027237078787418205
50-54	0.027748625058388498
55-59	0.02432972947758705
60-64	0.052882475832428846
65-69	0.09020879276276111
70-74	0.014685794203647124
75-79	0.016542449554039294
80-84	0.0027951035453376745
85-89	0.012503442126773862
90-94	0.01517157915161891
95-99	0.008547238952003619
100-101	0.0010672548099380191
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.0869007E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	11.112324448538141
#Duplication Level	Relative count
1	100.0
2	5.359021883696459
3	0.39953636688975896
4	0.20510957872415433
5	0.1490249282917684
6	0.12392037047917658
7	0.09080371974767248
8	0.08172334777290524
9	0.06249432476751577
10++	0.3562710651276326
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC	477524	4.39344642983485	TruSeq Adapter, Index 5 (100% over 50bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	12626380	5.1180778	21.879183	65-69
CTGGG	1984235	3.4585724	3.7417414	1
CACAC	2909830	3.427267	13.795973	30-34
CCCAG	2034170	3.4071524	3.934768	25-29
TTTTT	6561250	3.3592737	3.9205124	15-19
CCTCC	1774460	3.1250007	3.6066837	70-74
CCTGG	1753530	3.0669758	3.296629	70-74
GGAAG	2626890	3.06226	61.69405	5
GGAGG	1840440	3.0510259	3.4609761	4
CCAGG	1812565	3.0255516	3.4297068	25-29
CTCCA	2099565	2.5911694	14.507771	20-24
CAGTG	2095515	2.5684462	14.379246	35-39
TCCAG	2050035	2.521357	14.192118	25-29
CACAG	2083020	2.4450085	14.001747	30-34
CTGCT	1890370	2.4361615	15.018838	55-59
AGAGC	2010390	2.3516562	60.94268	8
TCTGC	1810795	2.3336115	14.915581	55-59
TTCTG	2389130	2.2608292	11.289177	55-59
GAGCA	1929030	2.2564852	60.821323	9
GCACA	1903240	2.2339861	13.244396	10-14
GAAGA	2720210	2.2298646	43.38034	6
AGCAC	1889875	2.2182987	13.119656	10-14
AAGAG	2661285	2.1815617	43.28973	7
CTTCT	2250530	2.1370082	11.471727	50-54
ACACA	2578005	2.1278777	10.0407	30-34
ACTCC	1677505	2.0702858	14.102466	20-24
GTCTG	1599780	2.0545945	14.330257	15-19
TCTTC	2067135	1.962864	11.353002	50-54
CGTCT	1502890	1.9368075	13.488655	15-19
CCAGT	1565775	1.9257611	13.774099	25-29
TGCTT	1993170	1.886133	11.301661	55-59
GAAAA	3246090	1.8711672	7.450244	60-64
GATCG	1525985	1.8703805	63.31105	1
TCTGA	2057800	1.8584249	10.673142	15-19
CTGAA	2139520	1.8440472	10.176448	15-19
ATGCC	1471920	1.810328	13.78502	45-49
GCTTG	1397775	1.79516	14.429228	55-59
AGTGA	2026550	1.7406826	9.980137	35-39
ATCGG	1403345	1.7200623	63.256344	2
TCACA	1985615	1.7172917	9.949882	30-34
CAGTC	1379220	1.6963155	13.554235	25-29
TCGGA	1356995	1.6632516	63.20224	3
TGAAA	2725010	1.645911	7.4650903	60-64
ATCTC	1804695	1.6354564	10.109447	40-44
CTTGA	1809685	1.6343491	10.36236	60-64
CGGAA	1350910	1.5802288	60.331158	4
GTCTT	1661330	1.5721134	10.804132	50-54
GTCAC	1272085	1.5645491	13.614001	25-29
AGATC	1797390	1.5491658	6.5494123	95-97
AACTC	1715200	1.4834188	9.819832	20-24
TGAAC	1708965	1.4729527	9.650281	20-24
GTGAT	1632395	1.4691755	10.094617	35-39
TTGAA	2305090	1.4588555	7.6264997	60-64
ACAGT	1688205	1.4550599	9.841981	30-34
GAACT	1645840	1.4185452	9.686728	20-24
AGTCA	1608795	1.3866165	9.868408	25-29
TGATC	1455150	1.3141639	9.952772	35-39
CCGTC	732145	1.2849542	17.955198	50-54
CACGT	1044625	1.284794	13.078234	10-14
GATCT	1421365	1.2836524	9.903889	35-39
TGCCG	707405	1.2372723	18.592226	45-49
ACACG	1047685	1.2297524	12.478316	10-14
GCCGT	669885	1.1716486	18.267666	45-49
TATGC	1224285	1.1056669	9.865459	45-49
GTATG	1221120	1.099023	9.71129	45-49
ACGTC	886445	1.090247	12.69372	15-19
TCTCG	841915	1.0849944	13.330855	40-44
CTCGT	712900	0.91872996	13.204095	40-44
CGTAT	688010	0.6213504	9.456603	40-44
TCGTA	649040	0.58615613	9.5089035	40-44
>>END_MODULE