FastQCFastQC Report
Thu 31 Jul 2014
ZL2_TGACCA_L008_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename ZL2_TGACCA_L008_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 14086255
Filtered Sequences 0
Sequence length 101
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 2323855 4.1022177 6.765929 40-44
AAAAA 11572645 3.9550157 5.1125884 1
TTTTT 10411010 3.7457268 4.684907 10-14
CCCAG 2568260 3.428071 3.8239522 25-29
CCTCC 2374090 3.2542195 3.736694 70-74
CCCCC 1698620 3.2529135 4.438412 95-97
GGAGG 2548750 3.2397995 3.583336 3
CTGGG 2481130 3.2387638 3.4404218 75-79
CCAGG 2299320 3.0195134 3.3655074 25-29