FastQCFastQC Report
Thu 31 Jul 2014
ZL2_TGACCA_L008_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename ZL2_TGACCA_L008_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 14086255
Filtered Sequences 0
Sequence length 101
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC 38868 0.27592855588657167 TruSeq Adapter, Index 4 (100% over 50bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 10431750 3.6380048 4.600343 65-69
TTTTT 10239585 3.6139174 4.3050256 15-19
CCCAG 2570655 3.4200063 3.9514103 25-29
CTGGG 2491270 3.256605 3.4744995 1
GGAGG 2510185 3.24096 3.6662676 4
CCTCC 2391050 3.2206838 3.7373269 70-74
GGGGG 1746365 3.1276517 3.816115 65-69
CCAGG 2306820 3.038494 3.3851418 25-29
GGAAG 2249755 2.0940533 6.0338893 5
GAGCA 1485975 1.3970191 5.268714 9
AGAGC 1474665 1.3863863 5.2170577 8
Produced by FastQC (version 0.10.1)