##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL2_TGACCA_L008_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	14086255	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.20742156094718	34.0	33.0	34.0	31.0	34.0
2	33.3429054067245	34.0	34.0	34.0	31.0	34.0
3	33.36297979839212	34.0	34.0	34.0	31.0	34.0
4	36.61172071640049	37.0	37.0	37.0	35.0	37.0
5	36.553182801248454	37.0	37.0	37.0	35.0	37.0
6	36.53527051725246	37.0	37.0	37.0	35.0	37.0
7	36.536881307345354	37.0	37.0	37.0	35.0	37.0
8	36.542439349564525	37.0	37.0	37.0	35.0	37.0
9	38.39087855501693	39.0	39.0	39.0	37.0	39.0
10-14	38.69275630747846	39.4	39.2	39.4	37.2	39.4
15-19	39.94835065814157	41.0	40.0	41.0	38.0	41.0
20-24	39.867184216102864	41.0	40.0	41.0	38.0	41.0
25-29	39.67656115837744	41.0	40.0	41.0	37.6	41.0
30-34	39.48408984502978	41.0	40.0	41.0	37.0	41.0
35-39	39.251141101733566	41.0	39.0	41.0	36.0	41.0
40-44	39.00869069884082	40.2	39.0	41.0	35.2	41.0
45-49	38.83188508230186	40.0	38.6	41.0	35.0	41.0
50-54	38.76477953863535	40.0	38.6	41.0	35.0	41.0
55-59	38.398747360458835	40.0	37.8	41.0	34.4	41.0
60-64	37.87629129246915	39.8	36.6	41.0	33.8	41.0
65-69	37.17367300251202	38.8	35.2	40.8	33.0	41.0
70-74	36.65344233793865	37.4	35.0	40.0	33.0	41.0
75-79	35.51837482709208	36.0	34.6	39.0	32.0	40.0
80-84	35.17758264350603	35.4	35.0	37.2	32.6	39.2
85-89	34.46299920028425	35.0	35.0	36.2	32.0	37.6
90-94	33.96592104856826	35.0	34.4	35.6	31.4	36.6
95-99	33.66209633433443	35.0	34.0	35.0	31.0	36.0
100-101	33.31257921285679	35.0	34.0	35.0	30.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	10.0
11	12.0
12	49.0
13	117.0
14	511.0
15	1551.0
16	3771.0
17	7208.0
18	10844.0
19	14196.0
20	17070.0
21	19724.0
22	23582.0
23	27714.0
24	32881.0
25	39304.0
26	48300.0
27	58670.0
28	72521.0
29	93275.0
30	118151.0
31	134669.0
32	169526.0
33	232230.0
34	361183.0
35	621566.0
36	1293223.0
37	2990241.0
38	5246369.0
39	2431377.0
40	16406.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.505132032731364	30.629672611815227	29.5971038920444	20.268091463409007
2	20.81547459309334	30.3134349126835	29.76206804992922	19.109022444293934
3	20.834224568559918	29.207138448082905	29.893978207834515	20.064658775522666
4	21.051003265239768	28.92773842302301	29.700853775542186	20.320404536195035
5	21.694353822218893	29.333744135684043	29.432365096329722	19.539536945767345
6	21.37512064065289	29.63305009031854	28.924295350325547	20.06753391870302
7	21.259965831940427	29.883854864192077	29.24340784686916	19.612771456998328
8	20.695159927177237	29.8872269456999	29.374173618183114	20.04343950893974
9	20.77462036573951	29.39739483631384	29.744023517961303	20.083961279985346
10-14	20.500513602453626	29.61547875562006	29.6525604736771	20.23144716824921
15-19	20.534169207034513	29.324795847794	29.701924099072137	20.439110846099346
20-24	20.715967422386598	29.219644684008344	29.484071077737394	20.580316815867665
25-29	20.895793186871657	29.10956097636479	29.27249339640184	20.722152440361715
30-34	20.94221193400065	29.063389745918304	29.21148424752229	20.782914072558754
35-39	21.082189327295232	29.04395637061303	29.02038167767309	20.853472624418643
40-44	21.083986723778875	28.93421910962263	29.09241055529645	20.889383611302044
45-49	21.22554648804202	28.88194796064731	29.02584287509787	20.866662676212798
50-54	21.122306323641418	28.835297227545638	28.964756759845876	21.077639688967068
55-59	21.16289512437924	28.816544703959373	29.035574862570062	20.98498530909132
60-64	21.11536532185501	28.865271495820426	28.959173520148994	21.060189662175567
65-69	21.068995198634788	29.098454928245527	28.794260187682603	21.038289685437086
70-74	21.1257257574803	29.104018975517697	28.752867992226772	21.017387274775228
75-79	21.112772534564815	29.05570504358875	28.799482513677514	21.03203990816893
80-84	21.12829520118108	29.067102956278628	28.796537134609522	21.008064707930778
85-89	21.11513429509639	29.079419687326773	28.758722970162065	21.046723047414773
90-94	21.127972329932398	29.083413600823494	28.681244111135268	21.107369958108844
95-99	21.19944539967339	29.1923991655747	28.529074420426713	21.079081014325197
100-101	21.302067668645822	29.23450657727413	28.361427111963867	21.101998642116186
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	39.77322349614037
2	39.92449703738728
3	40.898883344082584
4	41.3714078014348
5	41.23389076798624
6	41.442654559355915
7	40.87273728893875
8	40.73859943611698
9	40.858581645724854
10-14	40.73196077070284
15-19	40.97328005313386
20-24	41.29628423825426
25-29	41.61794562723337
30-34	41.72512600655941
35-39	41.935661951713875
40-44	41.973370335080915
45-49	42.09220916425482
50-54	42.199946012608486
55-59	42.14788043347056
60-64	42.175554984030576
65-69	42.10728488407187
70-74	42.14311303225553
75-79	42.144812442733745
80-84	42.13635990911185
85-89	42.16185734251116
90-94	42.23534228804124
95-99	42.278526413998584
100-101	42.404066310762005
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	256.0
1	236.5
2	267.5
3	355.5
4	456.5
5	528.5
6	620.0
7	810.0
8	1053.0
9	1340.0
10	1780.5
11	2523.5
12	3556.5
13	4878.5
14	6651.5
15	8991.5
16	12036.5
17	16082.0
18	21517.5
19	29164.5
20	39509.5
21	52911.0
22	70441.5
23	92138.5
24	117416.5
25	146585.5
26	179906.0
27	215154.5
28	251811.0
29	289251.0
30	325680.5
31	360087.5
32	392877.5
33	424140.5
34	450612.5
35	473170.5
36	492367.5
37	506508.0
38	513656.5
39	513363.0
40	506405.5
41	494510.0
42	479930.5
43	462940.0
44	446385.0
45	432062.5
46	419526.5
47	409523.5
48	398778.5
49	385340.0
50	368839.5
51	348623.0
52	327072.5
53	305526.0
54	285216.0
55	267269.0
56	249925.5
57	230991.5
58	209230.5
59	184904.5
60	159140.0
61	133194.0
62	110130.5
63	90607.5
64	74080.0
65	59975.5
66	47993.0
67	38407.5
68	30543.5
69	23715.0
70	18174.5
71	13973.5
72	10838.5
73	8627.0
74	6872.0
75	5497.5
76	4500.5
77	3715.5
78	3092.5
79	2497.0
80	1949.5
81	1513.5
82	1162.0
83	827.0
84	561.5
85	381.0
86	233.0
87	144.5
88	102.5
89	69.5
90	48.5
91	37.5
92	28.5
93	21.5
94	11.5
95	7.0
96	6.5
97	5.0
98	2.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0022433215925737535
2	0.00507587005914631
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	4.855797371267239E-4
15-19	0.0012182088141951142
20-24	4.7280132299181007E-4
25-29	0.013403136603731795
30-34	0.00848060751420445
35-39	0.02985321506674414
40-44	0.016381926920959473
45-49	0.018770070540395586
50-54	0.026320693470336862
55-59	0.019274107986828295
60-64	0.03839487500403762
65-69	0.07992330111871465
70-74	0.030371450751104534
75-79	0.017618593444460574
80-84	0.005750286360711203
85-89	0.018500304019769626
90-94	0.031102660004380153
95-99	0.014357258192472022
100-101	0.002601827100247724
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.4086255E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	5.061670585422238
#Duplication Level	Relative count
1	100.0
2	3.672940398671445
3	0.31849640314193817
4	0.16528231794021503
5	0.11805879852872501
6	0.09707056767917391
7	0.08972468688183101
8	0.06926116180351868
9	0.07031057334599623
10++	0.34000933976272807
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC	38868	0.27592855588657167	TruSeq Adapter, Index 4 (100% over 50bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	10431750	3.6380048	4.600343	65-69
TTTTT	10239585	3.6139174	4.3050256	15-19
CCCAG	2570655	3.4200063	3.9514103	25-29
CTGGG	2491270	3.256605	3.4744995	1
GGAGG	2510185	3.24096	3.6662676	4
CCTCC	2391050	3.2206838	3.7373269	70-74
GGGGG	1746365	3.1276517	3.816115	65-69
CCAGG	2306820	3.038494	3.3851418	25-29
GGAAG	2249755	2.0940533	6.0338893	5
GAGCA	1485975	1.3970191	5.268714	9
AGAGC	1474665	1.3863863	5.2170577	8
>>END_MODULE