##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL2_TGACCA_L007_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	14010096	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.77696648188564	34.0	31.0	34.0	31.0	34.0
2	32.89662376332039	34.0	33.0	34.0	31.0	34.0
3	32.89964765409174	34.0	33.0	34.0	31.0	34.0
4	36.18566054079858	37.0	37.0	37.0	35.0	37.0
5	36.183208237830776	37.0	37.0	37.0	35.0	37.0
6	36.22143252979851	37.0	37.0	37.0	35.0	37.0
7	36.191060004157	37.0	37.0	37.0	35.0	37.0
8	36.201361432498395	37.0	37.0	37.0	35.0	37.0
9	37.9769153616078	39.0	39.0	39.0	37.0	39.0
10-14	38.258517186463244	39.4	39.2	39.4	37.0	39.4
15-19	39.46303027473902	41.0	40.0	41.0	37.0	41.0
20-24	39.36084557878833	41.0	40.0	41.0	37.0	41.0
25-29	39.17787021587861	41.0	40.0	41.0	36.4	41.0
30-34	38.93804790488231	41.0	39.0	41.0	35.8	41.0
35-39	38.68344489573805	40.2	39.0	41.0	35.0	41.0
40-44	38.616376233253504	40.0	38.8	41.0	35.0	41.0
45-49	38.43190412114235	40.0	38.2	41.0	34.6	41.0
50-54	37.71710296631801	39.6	37.6	40.4	33.6	40.8
55-59	38.24102999722486	40.0	37.8	41.0	34.0	41.0
60-64	37.860289551192224	40.0	36.8	41.0	34.0	41.0
65-69	37.250699295707896	39.2	35.4	41.0	33.2	41.0
70-74	36.48362278174254	37.6	35.0	40.2	32.6	41.0
75-79	35.61998477383738	36.4	35.0	39.0	32.0	40.8
80-84	34.77032407201207	35.2	35.0	37.2	31.0	39.2
85-89	34.054803364659314	35.0	34.4	36.2	31.0	37.6
90-94	33.55987031066739	35.0	34.0	35.4	30.0	36.4
95-99	33.20805813179295	35.0	34.0	35.0	29.6	36.0
100-101	32.613865743675134	34.5	33.0	35.0	27.5	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	11.0
5	36.0
6	620.0
7	5296.0
8	12889.0
9	14051.0
10	14402.0
11	15197.0
12	15579.0
13	16886.0
14	18598.0
15	20088.0
16	21720.0
17	24268.0
18	26878.0
19	28727.0
20	30360.0
21	32111.0
22	34364.0
23	37255.0
24	41226.0
25	45828.0
26	52802.0
27	61420.0
28	72776.0
29	83150.0
30	100599.0
31	126797.0
32	165771.0
33	228697.0
34	354515.0
35	602606.0
36	1238912.0
37	2873250.0
38	5159970.0
39	2418412.0
40	14024.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.344115560907976	31.684048946098862	30.103729386445178	19.86810610654798
2	21.286504341018155	32.02302018284137	28.01335379190829	18.67712168423218
3	21.131333999849268	30.38634706763351	28.74802518758709	19.734293744930138
4	21.173848800647715	29.29930268953629	29.045110627470855	20.481737882345143
5	21.39710558745819	29.722575053716287	29.42818443764929	19.452134921176235
6	21.335677212767877	29.94399487253175	28.51178431855003	20.208543596150346
7	21.369336755840013	29.808224266988642	29.273946589079188	19.548492388092157
8	20.55653888394548	30.167736906295406	29.33133312560298	19.944391084156134
9	20.57919149267608	29.623784537212295	29.807766925276706	19.98925704483492
10-14	20.50306234271538	29.779419943243678	29.66318442235271	20.054333291688234
15-19	20.579343308528614	29.418203596699566	29.68668468153975	20.315768413232068
20-24	20.880999562576292	29.25970392143301	29.366636855908723	20.492659660081973
25-29	20.973502418699976	29.308691260971898	29.12685209878511	20.590954221543015
30-34	21.17941351978795	29.109665951294616	29.042967878096594	20.66795265082084
35-39	21.0951195797585	29.03304931496492	29.04705188271148	20.824779222565105
40-44	21.318829173343126	28.933251868861433	28.92527152999474	20.822647427800707
45-49	21.272238659700143	28.870105810734238	28.85890444282233	20.99875108674329
50-54	21.214646577360874	28.927695365026285	28.89441623040544	20.963241827207398
55-59	21.15913246622755	28.9715034195965	28.901593344286674	20.967770769889274
60-64	21.078575031732736	29.097939892701323	28.796520849696115	21.026964225869822
65-69	21.10359234736457	29.074685156973974	28.758002647114694	21.06371984854676
70-74	21.14365387816724	29.054848820345953	28.748784855848967	21.052712445637837
75-79	21.159255027858258	29.04944631328854	28.753396962248413	21.03790169660479
80-84	21.160062843271202	29.09109933986921	28.747585215161898	21.001252601697693
85-89	21.16052735521647	29.075708816674243	28.721208562612254	21.04255526549703
90-94	21.19101617547166	29.100447824455696	28.6456595776787	21.062876422393952
95-99	21.346019182719086	29.169711590880997	28.50006365583266	20.98420557056726
100-101	21.545584668553385	29.19411844631787	28.329811770547238	20.93048511458151
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	38.21222166745596
2	39.963626025250335
3	40.8656277447794
4	41.655586682992855
5	40.84924050863442
6	41.54422080891822
7	40.91782914393217
8	40.50092996810161
9	40.568448537510996
10-14	40.55739563440361
15-19	40.89511172176068
20-24	41.37365922265826
25-29	41.564456640242994
30-34	41.847366170608794
35-39	41.9198988023236
40-44	42.141476601143836
45-49	42.27098974644344
50-54	42.17788840456827
55-59	42.126903236116824
60-64	42.10553925760256
65-69	42.167312195911336
70-74	42.19636632380508
75-79	42.197156724463056
80-84	42.16131544496889
85-89	42.2030826207135
90-94	42.25389259786561
95-99	42.330224753286345
100-101	42.476069783134896
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	452.0
1	439.0
2	484.0
3	631.5
4	769.5
5	877.0
6	1038.0
7	1204.5
8	1456.5
9	1866.0
10	2420.5
11	3197.5
12	4170.5
13	5589.5
14	7406.5
15	9726.0
16	12860.0
17	16981.5
18	22684.0
19	30210.0
20	40289.0
21	54059.0
22	71249.5
23	92042.0
24	117224.0
25	146534.0
26	179163.5
27	214249.5
28	251243.5
29	287531.5
30	323209.5
31	358606.5
32	390713.5
33	419915.0
34	446227.5
35	469193.0
36	488264.0
37	501269.0
38	507195.5
39	505866.0
40	498849.5
41	487990.5
42	474018.5
43	458379.5
44	442331.5
45	428234.5
46	416912.0
47	407170.0
48	397023.0
49	383882.0
50	367044.5
51	347322.5
52	326140.0
53	305408.5
54	285722.0
55	266889.0
56	249256.0
57	230154.5
58	207642.0
59	183748.5
60	158541.5
61	132972.0
62	110334.5
63	90629.5
64	74048.5
65	60270.0
66	48497.5
67	38695.5
68	30525.0
69	23930.5
70	18609.5
71	14478.5
72	11416.0
73	8971.5
74	7042.5
75	5649.5
76	4647.5
77	3854.5
78	3179.0
79	2632.5
80	2082.5
81	1612.5
82	1280.5
83	1009.0
84	714.5
85	486.5
86	360.5
87	257.5
88	181.5
89	146.5
90	133.5
91	115.0
92	83.0
93	71.0
94	61.0
95	45.5
96	34.5
97	26.0
98	29.0
99	20.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07284746656982223
2	0.07905727412574474
3	0.08516001603415137
4	0.063325761650741
5	0.05917875223695826
6	0.006017089390393899
7	0.011777221226749623
8	0.011070587953144646
9	0.019821420210111337
10-14	0.020313922188684504
15-19	0.023548732285631733
20-24	0.018735060773316613
25-29	0.003301904569390531
30-34	0.004966418502771144
35-39	0.008827919523178143
40-44	0.022640815594696852
45-49	0.02054375644535198
50-54	0.02227821993510965
55-59	0.025204680967211075
60-64	0.02106909188916336
65-69	0.024620816302757666
70-74	0.02811401149570995
75-79	0.028927710416830836
80-84	0.025471631315017396
85-89	0.0205894377882921
90-94	0.023965574539960326
95-99	0.03636663160623596
100-101	0.03195552692858065
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.4010096E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	4.902029286405861
#Duplication Level	Relative count
1	100.0
2	3.747690822067344
3	0.3143630867411202
4	0.17738685028129986
5	0.12280628096397682
6	0.08659417247459904
7	0.08082122764295911
8	0.062452766815013855
9	0.0661264589806029
10++	0.30439163657737844
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	2277155	4.041713	6.1775556	40-44
AAAAA	11408380	3.9352221	5.1377463	8
TTTTT	10435880	3.7684116	4.684518	10-14
CCCAG	2558330	3.428652	3.8588276	25-29
CCCCC	1708650	3.2781217	4.521321	95-97
CTGGG	2471585	3.2404072	3.4725363	85-89
CCTCC	2357325	3.2383437	3.7300246	70-74
GGAGG	2528710	3.2343454	3.6338055	3
CCAGG	2291815	3.0240338	3.3646812	25-29
>>END_MODULE