FastQCFastQC Report
Thu 31 Jul 2014
ZL2_TGACCA_L007_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename ZL2_TGACCA_L007_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 14010096
Filtered Sequences 0
Sequence length 101
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC 34129 0.24360289893802298 TruSeq Adapter, Index 4 (100% over 50bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 10300885 3.6209216 4.470881 65-69
TTTTT 10090955 3.5906093 4.2873635 15-19
CCCAG 2562720 3.4218805 3.9221973 25-29
CTGGG 2481855 3.252368 3.4797869 85-89
GGAGG 2505740 3.2411692 3.6161087 4
CCTCC 2371105 3.2075396 3.6861222 70-74
GGGGG 1743245 3.1230536 3.8929684 65-69
CCAGG 2301480 3.040686 3.4259489 25-29
GGAAG 2234295 2.0866563 5.5533423 5
Produced by FastQC (version 0.10.1)