##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL2_TGACCA_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	14010096	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.209755736149134	34.0	33.0	34.0	31.0	34.0
2	33.35651290326633	34.0	34.0	34.0	31.0	34.0
3	33.382784885985075	34.0	34.0	34.0	31.0	34.0
4	36.631194104594286	37.0	37.0	37.0	35.0	37.0
5	36.57577792471943	37.0	37.0	37.0	35.0	37.0
6	36.5648359582975	37.0	37.0	37.0	35.0	37.0
7	36.565719749529194	37.0	37.0	37.0	35.0	37.0
8	36.56165725059985	37.0	37.0	37.0	35.0	37.0
9	38.42601670966423	39.0	39.0	39.0	37.0	39.0
10-14	38.728453552352526	39.4	39.2	39.4	37.2	39.4
15-19	39.99921085480071	41.0	40.0	41.0	38.0	41.0
20-24	39.92868094551243	41.0	40.0	41.0	38.0	41.0
25-29	39.749464000817696	41.0	40.0	41.0	38.0	41.0
30-34	39.571265578765484	41.0	40.0	41.0	37.2	41.0
35-39	39.350193888749935	41.0	39.6	41.0	36.6	41.0
40-44	39.12270843825767	40.8	39.0	41.0	35.6	41.0
45-49	38.94950344380224	40.0	39.0	41.0	35.0	41.0
50-54	38.87503413252843	40.0	38.8	41.0	35.0	41.0
55-59	38.52511904272462	40.0	37.8	41.0	35.0	41.0
60-64	38.00469694140568	39.8	36.8	41.0	34.0	41.0
65-69	37.30147149598404	39.0	35.4	40.8	33.2	41.0
70-74	36.773440738735836	37.6	35.0	40.0	33.0	41.0
75-79	35.64309907655165	36.2	34.6	39.0	32.4	40.0
80-84	35.293718872447414	35.4	35.0	37.2	33.0	39.2
85-89	34.59748666961311	35.0	35.0	36.2	32.6	37.6
90-94	34.09970384214355	35.0	35.0	35.6	32.0	36.6
95-99	33.806980037824154	35.0	34.4	35.0	32.0	36.0
100-101	33.47840143279532	35.0	34.0	35.0	30.5	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	13.0
11	12.0
12	30.0
13	154.0
14	527.0
15	1643.0
16	4025.0
17	7356.0
18	11157.0
19	14093.0
20	16161.0
21	18669.0
22	21688.0
23	25559.0
24	30647.0
25	36950.0
26	44123.0
27	53277.0
28	66457.0
29	84893.0
30	107195.0
31	122008.0
32	152545.0
33	208302.0
34	326910.0
35	567011.0
36	1210671.0
37	2919316.0
38	5348171.0
39	2591981.0
40	18545.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.514588518434532	30.661409599493737	29.560296280688505	20.26370560138323
2	20.872275121790203	30.262713052546037	29.747512156177	19.117499669486758
3	20.866266841610948	29.202991849844473	29.85430049863688	20.076440809907698
4	21.0456305750143	28.929553711903168	29.697966396390846	20.326849316691682
5	21.66776730152313	29.320227356043816	29.447564099489398	19.56444124294366
6	21.393250981292347	29.591981382568683	28.930586913894096	20.084180722244874
7	21.283679997624567	29.822400931442584	29.267351201590625	19.626567869342225
8	20.702720381073764	29.837811246975036	29.37179730959731	20.08767106235389
9	20.751991992060585	29.36539478387586	29.785070709008703	20.097542515054858
10-14	20.52028517169702	29.576496532569465	29.66141820970978	20.24180008602374
15-19	20.536389005154586	29.299241915949516	29.701849871263896	20.462519207632006
20-24	20.731699291322336	29.203034171279718	29.47595489454633	20.58931164285162
25-29	20.909506774713957	29.10621301332896	29.25780856431985	20.726471647637233
30-34	20.966886202304714	29.043701904081736	29.211552621275505	20.777859272338045
35-39	21.100132997885318	29.039397187445363	29.010853271175783	20.84961654349353
40-44	21.106184262824932	28.924429853492605	29.080703208536452	20.888682675146015
45-49	21.218469443834877	28.870579142234305	29.02133430600134	20.889617107929478
50-54	21.12035711100895	28.845555785850774	28.96148178050299	21.07260532263729
55-59	21.158632629168565	28.817770795211377	29.029723746219787	20.993872829400264
60-64	21.122529307795897	28.861848485231533	28.95747281753841	21.05814938943416
65-69	21.094425886739828	29.068538335318006	28.781667922091465	21.055367855850704
70-74	21.13724521002558	29.07601851740804	28.761835373023775	21.024900899542605
75-79	21.12633944869156	29.040695830341	28.80218231047001	21.03078241049743
80-84	21.137620432191476	29.04640293004086	28.79735099893655	21.018625638831114
85-89	21.113786697104214	29.05370813974708	28.77105881790586	21.061446345242842
90-94	21.13427738682674	29.061421815257926	28.699450928583026	21.104849869332313
95-99	21.203722681096533	29.17040596390093	28.537505577180266	21.088365777822272
100-101	21.309631811151093	29.20910739739599	28.38630528489436	21.094955506558563
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	39.778294119817765
2	39.98977479127696
3	40.942707651518646
4	41.37247989170598
5	41.23220854446679
6	41.47743170353722
7	40.91024786696679
8	40.79039144342765
9	40.84953450711544
10-14	40.762085257720756
15-19	40.99890821278659
20-24	41.321010934173955
25-29	41.635978422351194
30-34	41.744745474642755
35-39	41.94974954137884
40-44	41.99486693797094
45-49	42.10808655176436
50-54	42.192962433646244
55-59	42.152505458568825
60-64	42.180678697230064
65-69	42.14979374259053
70-74	42.162146109568184
75-79	42.15712185918898
80-84	42.15624607102259
85-89	42.17523304234705
90-94	42.23912725615905
95-99	42.292088458918805
100-101	42.404587317709655
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	238.0
1	225.5
2	271.0
3	388.0
4	468.5
5	520.0
6	610.0
7	778.5
8	1014.0
9	1325.0
10	1844.5
11	2585.0
12	3526.0
13	4889.0
14	6592.5
15	8765.0
16	11878.0
17	15813.0
18	21255.5
19	28983.5
20	39278.0
21	52818.0
22	69865.5
23	91043.0
24	116368.0
25	145734.5
26	178424.0
27	213228.5
28	249142.5
29	285975.5
30	323325.5
31	358436.0
32	390991.0
33	420950.5
34	447716.0
35	470663.5
36	489593.0
37	503163.5
38	509596.5
39	509971.0
40	503514.5
41	491642.5
42	477209.5
43	460172.5
44	444245.5
45	430442.5
46	417087.5
47	406761.5
48	397259.0
49	383908.5
50	367049.0
51	347741.0
52	325438.5
53	304171.0
54	284629.0
55	266164.5
56	249200.5
57	230452.0
58	209302.5
59	185231.0
60	158867.5
61	133085.0
62	110304.0
63	90837.0
64	73943.5
65	60071.0
66	48507.0
67	38416.0
68	30035.0
69	23349.5
70	18050.0
71	13845.5
72	10805.5
73	8487.0
74	6695.5
75	5367.0
76	4270.5
77	3483.0
78	2890.0
79	2365.0
80	1865.5
81	1408.0
82	1068.0
83	780.5
84	543.5
85	367.5
86	222.0
87	116.0
88	72.5
89	58.5
90	45.0
91	30.0
92	22.5
93	19.5
94	16.5
95	14.5
96	7.5
97	3.0
98	1.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.1413129503181135E-5
2	0.011277581538342064
3	0.002155588369986901
4	0.0059028860330435995
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	7.137709834393711E-5
15-19	2.8550839337574844E-4
20-24	0.002236958262098989
25-29	0.011687286082836262
30-34	0.007002093347540231
35-39	0.03075924676033626
40-44	0.02487920139876272
45-49	0.027191819385106283
50-54	0.027972684840988953
55-59	0.024286771482508043
60-64	0.05419663077255145
65-69	0.09260893001732465
70-74	0.01490353813421407
75-79	0.016677972799044347
80-84	0.002907902986531998
85-89	0.012730819260624623
90-94	0.015121952055146517
95-99	0.008742267005165418
100-101	0.001184859832509356
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.4010096E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	5.117886705248381
#Duplication Level	Relative count
1	100.0
2	3.713190908613721
3	0.29657235840638285
4	0.1732192535824891
5	0.13175161408849928
6	0.10445645897853131
7	0.08923416093643378
8	0.08083565167182825
9	0.058789564852238727
10++	0.33331583643903206
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC	34129	0.24360289893802298	TruSeq Adapter, Index 4 (100% over 50bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	10300885	3.6209216	4.470881	65-69
TTTTT	10090955	3.5906093	4.2873635	15-19
CCCAG	2562720	3.4218805	3.9221973	25-29
CTGGG	2481855	3.252368	3.4797869	85-89
GGAGG	2505740	3.2411692	3.6161087	4
CCTCC	2371105	3.2075396	3.6861222	70-74
GGGGG	1743245	3.1230536	3.8929684	65-69
CCAGG	2301480	3.040686	3.4259489	25-29
GGAAG	2234295	2.0866563	5.5533423	5
>>END_MODULE