FastQCFastQC Report
Thu 31 Jul 2014
ZL1_CGATGT_L008_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename ZL1_CGATGT_L008_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 18026677
Filtered Sequences 0
Sequence length 101
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 2817785 3.957 9.846357 40-44
CCCAG 3489080 3.715444 4.2251544 25-29
AAAAA 13943890 3.6519933 5.047635 60-64
CTGGG 3364720 3.480863 3.7246063 85-89
TTTTT 12103690 3.3414516 4.3199835 10-14
CCTCC 3007280 3.3007753 3.8409488 70-74
GGAGG 3279710 3.291783 3.5688734 70-74
CCAGG 3129445 3.2673757 3.6640444 25-29
CCTGG 2953390 3.1162107 3.3669608 70-74
GGGAG 2939280 2.9501 5.888921 5
GGAAG 2828505 2.0290384 5.3342776 5
AGAGC 1901335 1.391105 5.4224644 8
GAGCG 451430 0.4621194 6.1657395 9
GATCG 540860 0.3999083 5.131256 1
ATCGG 442865 0.32745147 5.1110907 2