##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL1_CGATGT_L008_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	18026677	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72601672510136	34.0	31.0	34.0	31.0	34.0
2	32.832801686078916	34.0	33.0	34.0	31.0	34.0
3	32.82756328301661	34.0	33.0	34.0	31.0	34.0
4	36.11673798781661	37.0	37.0	37.0	35.0	37.0
5	36.10920398695777	37.0	37.0	37.0	35.0	37.0
6	36.168524404137266	37.0	37.0	37.0	35.0	37.0
7	36.110796515630696	37.0	37.0	37.0	35.0	37.0
8	36.1406138247221	37.0	37.0	37.0	35.0	37.0
9	37.89987949526138	39.0	39.0	39.0	37.0	39.0
10-14	38.176709451220546	39.4	39.2	39.4	37.0	39.4
15-19	39.36742713035797	41.0	40.0	41.0	37.0	41.0
20-24	39.29798926335674	41.0	40.0	41.0	37.0	41.0
25-29	39.09741014386623	41.0	39.6	41.0	36.2	41.0
30-34	38.853949976470986	41.0	39.0	41.0	35.8	41.0
35-39	38.6102255673633	40.0	39.0	41.0	35.0	41.0
40-44	38.54716406135196	40.0	38.8	41.0	35.0	41.0
45-49	38.39067084854297	40.0	38.2	41.0	34.6	41.0
50-54	37.728082618887555	39.6	37.8	40.4	33.6	40.8
55-59	38.313105671111764	40.0	38.0	41.0	34.2	41.0
60-64	37.974206471886085	40.0	37.2	41.0	34.0	41.0
65-69	37.364714528362605	39.2	36.0	41.0	33.2	41.0
70-74	36.558431662141615	38.0	35.0	40.4	32.4	41.0
75-79	35.6689443206865	36.6	35.0	39.0	31.8	40.8
80-84	34.76696734511857	35.4	35.0	37.4	31.0	39.2
85-89	34.02344109233221	35.0	34.2	36.2	30.6	37.6
90-94	33.5143025306328	35.0	34.0	35.6	30.0	36.6
95-99	33.149408190982726	35.0	34.0	35.0	29.2	36.0
100-101	32.52945409739132	34.5	33.0	35.0	27.0	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	26.0
4	92.0
5	167.0
6	851.0
7	5370.0
8	16586.0
9	25767.0
10	28770.0
11	27654.0
12	25704.0
13	25170.0
14	25692.0
15	27154.0
16	28493.0
17	31698.0
18	34129.0
19	36664.0
20	38240.0
21	40114.0
22	42386.0
23	46248.0
24	51052.0
25	57167.0
26	65884.0
27	77193.0
28	90334.0
29	105819.0
30	129632.0
31	163372.0
32	213584.0
33	296127.0
34	449691.0
35	748589.0
36	1498335.0
37	3613959.0
38	6814431.0
39	3128881.0
40	15652.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.45886426492049	31.762153976532037	29.97188040055423	19.807101357993247
2	21.142058236282395	32.22666012501498	28.163104096262437	18.468177542440188
3	20.949877209822038	30.441758688274746	29.118631985990444	19.489732115912773
4	20.9123942962669	29.408365592925083	29.165465706090693	20.51377440471732
5	21.45694190672708	29.775644812145753	29.515533717122068	19.251879564005105
6	21.418913695844623	29.93796624027648	28.651359637929318	19.991760425949575
7	21.260147270025264	29.998558613019554	29.369230637784334	19.372063479170848
8	20.348835112006253	30.48026416452043	29.432151615667905	19.738749107805408
9	20.66561012855731	29.66261268176524	29.910057725521433	19.76171946415602
10-14	20.526771513664315	29.810829756615657	29.776722417833472	19.885676311886556
15-19	20.5929426964168	29.38930103302633	29.845938247284202	20.171818023272667
20-24	20.958875383621592	29.315671477017947	29.437036014196543	20.288417125163917
25-29	20.92791611214229	29.513926802615913	29.17459644152769	20.383560643714105
30-34	21.24897798653425	29.166696209037536	29.131114434429033	20.453211369999185
35-39	20.96514884647334	29.125518425233587	29.205336071637618	20.703996656655452
40-44	21.446441165695248	28.918520401968483	29.011418620529096	20.62361981180717
45-49	21.30497821689352	28.862044853325152	28.92877861959992	20.90419831018141
50-54	21.183926535093452	28.938470470442606	29.015930916731225	20.861672077732717
55-59	20.993968556527687	29.07835213791258	29.11526860623689	20.812410699322843
60-64	20.894878065351083	29.412630578949457	28.845362886834486	20.84712846886497
65-69	20.926942162202433	29.364285880832057	28.81916614001108	20.889605816954425
70-74	20.983816938807415	29.3283405565316	28.801914242798055	20.885928261862933
75-79	21.009743925174163	29.28746010560025	28.815277750444956	20.887518218780627
80-84	21.013303918204475	29.301774451457213	28.81801256028903	20.86690907004928
85-89	21.021526739963946	29.252386932353318	28.803464257099716	20.92262207058302
90-94	21.038038401437696	29.25310486908468	28.752807004420955	20.95604972505667
95-99	21.153696302188965	29.322590403643645	28.632711706598684	20.89100158756871
100-101	21.302459867652797	29.347614749226285	28.49234440866838	20.857580974452535
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	38.26596562291373
2	39.61023577872258
3	40.43960932573481
4	41.42616870098422
5	40.70882147073219
6	41.4106741217942
7	40.63221074919611
8	40.08758421981166
9	40.42732959271333
10-14	40.41244782555087
15-19	40.76476071968946
20-24	41.24729250878551
25-29	41.3114767558564
30-34	41.70218935653343
35-39	41.66914550312879
40-44	42.07006097750242
45-49	42.20917652707492
50-54	42.04559861282617
55-59	41.80637925585053
60-64	41.74200653421605
65-69	41.81654797915686
70-74	41.869745200670344
75-79	41.89726214395479
80-84	41.88021298825376
85-89	41.94414881054696
90-94	41.99408812649437
95-99	42.04469788975768
100-101	42.16004084210533
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	434.0
1	429.0
2	501.0
3	659.5
4	812.0
5	921.0
6	1048.0
7	1258.5
8	1477.0
9	1744.5
10	2154.0
11	2736.0
12	3532.5
13	4524.5
14	5928.5
15	8139.0
16	11308.5
17	16139.0
18	23297.5
19	33068.5
20	46451.5
21	64492.5
22	87858.0
23	116614.0
24	151560.0
25	192291.5
26	236576.0
27	283874.0
28	333193.0
29	382777.0
30	431738.0
31	478890.5
32	522435.0
33	561946.5
34	596217.5
35	625393.5
36	648794.5
37	664360.0
38	671027.5
39	667705.5
40	656069.5
41	638425.0
42	617234.0
43	593760.5
44	570810.0
45	550925.5
46	533641.0
47	518721.5
48	504255.5
49	486847.5
50	464717.0
51	438722.5
52	409959.0
53	381452.0
54	355341.0
55	331331.5
56	308757.0
57	284939.0
58	257223.5
59	225340.0
60	191998.0
61	159703.0
62	130755.5
63	106561.0
64	86376.5
65	69899.5
66	56120.5
67	44464.0
68	35160.0
69	27604.5
70	21482.5
71	16782.5
72	13423.5
73	10696.0
74	8534.0
75	7024.0
76	5815.5
77	4828.0
78	3955.0
79	3131.5
80	2441.0
81	1905.5
82	1434.5
83	1033.0
84	744.0
85	547.0
86	397.0
87	279.5
88	205.0
89	164.5
90	135.5
91	111.5
92	94.5
93	77.0
94	64.0
95	49.5
96	36.0
97	31.0
98	22.5
99	13.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09724476674209008
2	0.08274403540929923
3	0.09256281676317826
4	0.09312864484119841
5	0.07760165670023376
6	0.035103530173642095
7	0.013152729146919312
8	0.04385722338065967
9	0.05182874247982587
10-14	0.04911498663896846
15-19	0.020792517667010953
20-24	0.01550923667185028
25-29	0.00815458112440801
30-34	0.009534757848049311
35-39	0.01160169453305232
40-44	0.025448949909070876
45-49	0.02394451290162907
50-54	0.03540974301586476
55-59	0.03925737394640177
60-64	0.026319881362494038
65-69	0.028264776697335844
70-74	0.02989680238903709
75-79	0.044629412287134226
80-84	0.047466318944972496
85-89	0.03267268837179475
90-94	0.04488458965565312
95-99	0.03490160721246628
100-101	0.05534020496400973
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.8026677E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	5.340534502276548
#Duplication Level	Relative count
1	100.0
2	3.9359583592358836
3	0.34665514661721475
4	0.17543489942786095
5	0.1253859040955883
6	0.10800046361174624
7	0.0990443276049185
8	0.06111245745835397
9	0.06690760428630133
10++	0.4478068003413868
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	2817785	3.957	9.846357	40-44
CCCAG	3489080	3.715444	4.2251544	25-29
AAAAA	13943890	3.6519933	5.047635	60-64
CTGGG	3364720	3.480863	3.7246063	85-89
TTTTT	12103690	3.3414516	4.3199835	10-14
CCTCC	3007280	3.3007753	3.8409488	70-74
GGAGG	3279710	3.291783	3.5688734	70-74
CCAGG	3129445	3.2673757	3.6640444	25-29
CCTGG	2953390	3.1162107	3.3669608	70-74
GGGAG	2939280	2.9501	5.888921	5
GGAAG	2828505	2.0290384	5.3342776	5
AGAGC	1901335	1.391105	5.4224644	8
GAGCG	451430	0.4621194	6.1657395	9
GATCG	540860	0.3999083	5.131256	1
ATCGG	442865	0.32745147	5.1110907	2
>>END_MODULE