FastQCFastQC Report
Thu 31 Jul 2014
ZL1_CGATGT_L008_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename ZL1_CGATGT_L008_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 18026677
Filtered Sequences 0
Sequence length 101
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC 105894 0.5874293969986815 TruSeq Adapter, Index 2 (100% over 50bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CCCAG 3506665 3.7074013 4.2646933 25-29
CTGGG 3378370 3.5237617 3.7974083 1
AAAAA 12912885 3.4282014 5.5691395 65-69
CCAGG 3144860 3.2948084 3.7009623 25-29
GGAGG 3193990 3.2860138 3.647401 70-74
CCTCC 3023410 3.2406776 3.8093834 70-74
TTTTT 11757620 3.1948285 3.8546913 10-14
CCTGG 2963690 3.119454 3.4029055 70-74
GGAAG 2823665 2.0705028 10.163666 5
GAAGA 2985910 1.5605087 7.3205047 6
AAGAG 2973295 1.5539156 7.2477856 7
AGAGC 1964265 1.4534806 9.472243 8
GAGCA 1897235 1.403881 9.438411 9
GATCG 585635 0.43536466 8.510691 1
CGGAA 554650 0.41041967 8.5004015 4
TCGGA 492190 0.3658971 8.500688 3
ATCGG 476955 0.35457128 8.47807 2
Produced by FastQC (version 0.10.1)