##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL1_CGATGT_L008_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	18026677	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.207725250749206	34.0	33.0	34.0	31.0	34.0
2	33.34283922655296	34.0	34.0	34.0	31.0	34.0
3	33.36275626395258	34.0	34.0	34.0	31.0	34.0
4	36.61281294383873	37.0	37.0	37.0	35.0	37.0
5	36.554386756915875	37.0	37.0	37.0	35.0	37.0
6	36.53739688129986	37.0	37.0	37.0	35.0	37.0
7	36.538003870596896	37.0	37.0	37.0	35.0	37.0
8	36.545219620898514	37.0	37.0	37.0	35.0	37.0
9	38.3943922665281	39.0	39.0	39.0	37.0	39.0
10-14	38.6993934378477	39.4	39.2	39.4	37.2	39.4
15-19	39.961301065082594	41.0	40.0	41.0	38.0	41.0
20-24	39.89011051787304	41.0	40.0	41.0	38.0	41.0
25-29	39.71254895175633	41.0	40.0	41.0	37.6	41.0
30-34	39.53476127630178	41.0	40.0	41.0	37.0	41.0
35-39	39.31036016233053	41.0	39.2	41.0	36.6	41.0
40-44	39.10311967091883	40.4	39.0	41.0	35.8	41.0
45-49	38.95676408913302	40.0	38.8	41.0	35.0	41.0
50-54	38.92264787348218	40.0	39.0	41.0	35.0	41.0
55-59	38.59254472690669	40.0	38.0	41.0	35.0	41.0
60-64	38.09415847413253	39.8	37.0	41.0	34.0	41.0
65-69	37.39585604157661	39.0	35.8	41.0	33.0	41.0
70-74	36.82486782228361	37.8	35.0	40.0	33.0	41.0
75-79	35.635917790061924	36.2	34.6	39.0	32.0	40.0
80-84	35.2519521040955	35.6	35.0	37.4	32.8	39.2
85-89	34.504240531962715	35.0	35.0	36.4	32.0	37.8
90-94	33.98967833062078	35.0	34.6	35.6	31.8	36.6
95-99	33.681499457720356	35.0	34.0	35.0	31.0	36.0
100-101	33.341206146867776	35.0	34.0	35.0	30.5	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	1.0
9	1.0
10	8.0
11	22.0
12	45.0
13	153.0
14	655.0
15	1785.0
16	4542.0
17	8645.0
18	12965.0
19	16398.0
20	19544.0
21	22580.0
22	27134.0
23	32340.0
24	38800.0
25	47448.0
26	58682.0
27	73191.0
28	94058.0
29	130485.0
30	164091.0
31	164115.0
32	202581.0
33	277172.0
34	423738.0
35	718860.0
36	1481982.0
37	3682836.0
38	7001876.0
39	3299522.0
40	20420.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.464779448594165	30.83907463620694	29.535187322089325	20.16095859310957
2	20.70075978534928	30.761068513713358	29.692685724182667	18.8454859767547
3	20.709679326921986	29.28946360995984	30.176066282210527	19.82479078090765
4	20.832724744554973	29.01311206718798	29.68545450722837	20.468708681028676
5	21.768504533586526	29.40951901451388	29.520388033801236	19.301588418098355
6	21.475832733897658	29.664585436350805	29.00321007582263	19.856371753928915
7	21.000254234321723	30.253307362194377	29.324234300087586	19.422204103396318
8	20.45275454816215	30.249579553680363	29.42209482091458	19.87557107724291
9	20.884442540352836	29.40992396990305	29.84756979891524	19.858063690828875
10-14	20.39014615955217	29.749686737719717	29.695470154368316	20.1646969483598
15-19	20.407027971891033	29.400114829064538	29.814876549214087	20.377980649830345
20-24	20.591451785333142	29.380154595225278	29.562732713237335	20.465660906204246
25-29	20.786799033363508	29.213641484722157	29.340853565152813	20.658705916761523
30-34	20.77785014975407	29.151564334821323	29.226881589060028	20.84370392636458
35-39	21.099349235417286	29.06562615242139	29.29274746538459	20.542277146776726
40-44	20.903479915244365	29.030538534023464	29.23769828822809	20.828283262504087
45-49	21.168332484708525	28.957295612778967	29.180772175892322	20.693599726620192
50-54	21.004199305508436	28.86361287205805	29.042744606011283	21.08944321642223
55-59	21.04305232583785	28.839325132811055	29.247538764149084	20.870083777202012
60-64	20.994181714166203	28.97460018083792	29.088268179093784	20.94294992590209
65-69	20.892231901307543	29.45515077610723	28.796005872577695	20.856611450007527
70-74	20.95423281971139	29.436593714422344	28.766569539260246	20.842603926606024
75-79	20.957523053085605	29.32892629341023	28.815196020734255	20.89835463276991
80-84	20.97401529515066	29.308848529428765	28.825319372386264	20.89181680303431
85-89	20.950811617706787	29.272461733453703	28.813022822183772	20.96370382665574
90-94	20.957383307076057	29.2632516097714	28.752249939889694	21.027115143262844
95-99	21.00684161121005	29.337443780142213	28.648950671398183	21.00676393724956
100-101	21.105959574405706	29.36842556944177	28.497727630602267	21.02788722555026
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	39.625738041703734
2	39.54624576210398
3	40.534470107829634
4	41.30143342558365
5	41.070092951684884
6	41.332204487826566
7	40.422458337718034
8	40.32832562540506
9	40.74250623118171
10-14	40.55484310791197
15-19	40.785008621721374
20-24	41.057112691537384
25-29	41.44550495012503
30-34	41.62155407611865
35-39	41.64162638219401
40-44	41.73176317774845
45-49	41.861932211328714
50-54	42.09364252193067
55-59	41.91313610303986
60-64	41.937131640068294
65-69	41.748843351315074
70-74	41.79683674631741
75-79	41.85587768585551
80-84	41.86583209818497
85-89	41.91451544436253
90-94	41.984498450338904
95-99	42.01360554845961
100-101	42.13384679995597
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	263.0
1	247.0
2	263.0
3	367.0
4	476.0
5	548.0
6	653.5
7	806.5
8	979.0
9	1203.5
10	1566.0
11	2014.5
12	2686.5
13	3657.0
14	5035.5
15	7075.5
16	9993.0
17	14611.0
18	21511.0
19	31205.5
20	44689.5
21	63051.5
22	86597.0
23	115281.5
24	149721.0
25	190160.5
26	234547.0
27	282176.0
28	332465.5
29	382321.5
30	431078.0
31	477481.0
32	522080.0
33	562753.0
34	598408.0
35	628652.5
36	653205.5
37	671584.0
38	679472.5
39	677054.0
40	665158.0
41	647184.5
42	625136.0
43	599600.0
44	574434.0
45	552218.5
46	532369.5
47	516842.0
48	502212.5
49	484149.5
50	462442.5
51	435869.5
52	406308.5
53	377946.0
54	352328.5
55	328900.5
56	307553.5
57	284315.0
58	256723.0
59	225027.0
60	191959.5
61	159670.5
62	130441.5
63	105902.0
64	85608.5
65	69228.0
66	55644.5
67	43887.5
68	34290.0
69	26990.5
70	20861.5
71	16190.0
72	12705.5
73	10133.5
74	8289.0
75	6678.0
76	5353.5
77	4412.0
78	3632.0
79	2857.5
80	2161.5
81	1611.0
82	1193.5
83	836.0
84	546.0
85	342.5
86	212.0
87	132.5
88	99.5
89	84.5
90	57.0
91	36.5
92	28.5
93	20.5
94	15.5
95	13.0
96	9.5
97	6.0
98	3.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0023631643258488514
2	0.005147926043163695
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	4.948222015627173E-4
15-19	0.0012781057762337451
20-24	5.092452702181328E-4
25-29	0.013565450803828125
30-34	0.008824699083475007
35-39	0.02986018998398873
40-44	0.01665531589654599
45-49	0.01886093593400492
50-54	0.026319881362494038
55-59	0.019504426689400382
60-64	0.03886129429178766
65-69	0.080728134198
70-74	0.030711151034658248
75-79	0.017813599256257823
80-84	0.005781431597182331
85-89	0.01879769632528502
90-94	0.03128696431405522
95-99	0.014526249069642729
100-101	0.0024879793430591783
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.8026677E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	5.76484783483039
#Duplication Level	Relative count
1	100.0
2	3.869155825337359
3	0.33301191360659277
4	0.1828404019327337
5	0.1348909017140629
6	0.1122334455667789
7	0.09537208285252104
8	0.07534921462933983
9	0.07745688496862206
10++	0.5032062935036331
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC	105894	0.5874293969986815	TruSeq Adapter, Index 2 (100% over 50bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCCAG	3506665	3.7074013	4.2646933	25-29
CTGGG	3378370	3.5237617	3.7974083	1
AAAAA	12912885	3.4282014	5.5691395	65-69
CCAGG	3144860	3.2948084	3.7009623	25-29
GGAGG	3193990	3.2860138	3.647401	70-74
CCTCC	3023410	3.2406776	3.8093834	70-74
TTTTT	11757620	3.1948285	3.8546913	10-14
CCTGG	2963690	3.119454	3.4029055	70-74
GGAAG	2823665	2.0705028	10.163666	5
GAAGA	2985910	1.5605087	7.3205047	6
AAGAG	2973295	1.5539156	7.2477856	7
AGAGC	1964265	1.4534806	9.472243	8
GAGCA	1897235	1.403881	9.438411	9
GATCG	585635	0.43536466	8.510691	1
CGGAA	554650	0.41041967	8.5004015	4
TCGGA	492190	0.3658971	8.500688	3
ATCGG	476955	0.35457128	8.47807	2
>>END_MODULE