##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL1_CGATGT_L007_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	17934766	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.77643332508492	34.0	31.0	34.0	31.0	34.0
2	32.884625982853635	34.0	33.0	34.0	31.0	34.0
3	32.883775456005395	34.0	33.0	34.0	31.0	34.0
4	36.16467413068004	37.0	37.0	37.0	35.0	37.0
5	36.165734696510675	37.0	37.0	37.0	35.0	37.0
6	36.22187716304746	37.0	37.0	37.0	35.0	37.0
7	36.163762995290824	37.0	37.0	37.0	35.0	37.0
8	36.19591312203349	37.0	37.0	37.0	35.0	37.0
9	37.95514449421866	39.0	39.0	39.0	37.0	39.0
10-14	38.23829003400435	39.4	39.2	39.4	37.0	39.4
15-19	39.44598368331095	41.0	40.0	41.0	37.0	41.0
20-24	39.367756189291796	41.0	40.0	41.0	37.0	41.0
25-29	39.19002293088184	41.0	40.0	41.0	36.6	41.0
30-34	38.94993604042562	41.0	39.2	41.0	36.0	41.0
35-39	38.72056506340813	40.4	39.0	41.0	35.2	41.0
40-44	38.68322000967284	40.0	39.0	41.0	35.0	41.0
45-49	38.518454771029624	40.0	38.8	41.0	35.0	41.0
50-54	37.83286798389229	39.6	38.0	40.4	33.8	40.8
55-59	38.40575943951541	40.0	38.4	41.0	34.8	41.0
60-64	38.07447178290478	40.0	37.4	41.0	34.0	41.0
65-69	37.469136279781964	39.4	36.2	41.0	33.6	41.0
70-74	36.68176288444466	38.0	35.0	40.4	33.0	41.0
75-79	35.78551658828446	36.6	35.0	39.0	32.0	40.8
80-84	34.89847032294706	35.6	35.0	37.4	31.4	39.2
85-89	34.151460175170385	35.0	34.6	36.4	31.0	37.6
90-94	33.6373144204948	35.0	34.0	35.6	30.6	36.6
95-99	33.28050443479441	35.0	34.0	35.0	30.0	36.0
100-101	32.69771897219066	35.0	33.5	35.0	28.5	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	18.0
5	62.0
6	514.0
7	4585.0
8	13202.0
9	20325.0
10	24624.0
11	25120.0
12	24699.0
13	24460.0
14	25136.0
15	26589.0
16	27970.0
17	30560.0
18	33619.0
19	34884.0
20	36398.0
21	38229.0
22	40310.0
23	43691.0
24	48060.0
25	53870.0
26	62238.0
27	73916.0
28	87572.0
29	99794.0
30	120724.0
31	152269.0
32	199213.0
33	274639.0
34	419073.0
35	702156.0
36	1428230.0
37	3541090.0
38	6888374.0
39	3291285.0
40	17266.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.396819382074618	31.766467284719642	29.985030869820463	19.85168246338528
2	21.133333872758374	32.209294312069844	28.202004261643836	18.45536755352794
3	20.970829521106136	30.411212705396384	29.11636968265807	19.501588090839412
4	20.950277869418027	29.40932851436732	29.156535327916156	20.4838582882985
5	21.406893562882953	29.774077888916523	29.564701060725806	19.254327487474715
6	21.385007282119265	29.948131593713782	28.650487438337297	20.016373685829652
7	21.230918422343287	29.99926111993585	29.392319941201876	19.37750051651899
8	20.339851930764215	30.427701540850645	29.447776162106614	19.784670366278526
9	20.633236053397816	29.666272007191985	29.916457857785396	19.7840340816248
10-14	20.49469002201702	29.82242497237248	29.781137896179864	19.90174710943063
15-19	20.570028315631987	29.416678706659695	29.85139449219983	20.161898485508488
20-24	20.915340060439693	29.325577059961894	29.450631470118545	20.30845140947987
25-29	20.912641441960737	29.48324722595566	29.19014955494259	20.41396177714101
30-34	21.20925589859329	29.166586743252775	29.14736163071243	20.476795727441502
35-39	20.958937667982056	29.11337304552633	29.21536953854929	20.712319747942328
40-44	21.40381967786728	28.938351385690915	29.01465214090175	20.643176795540054
45-49	21.269805566905525	28.873502973766406	28.93787406032039	20.91881739900768
50-54	21.16897618589241	28.962127335015513	29.0120840684778	20.85681241061428
55-59	21.00812139864042	29.07826666946717	29.09659447019767	20.817017461694746
60-64	20.9069674592092	29.369159153443203	28.862931398859658	20.860941988487944
65-69	20.945852800195922	29.31824311346926	28.823072407911376	20.91283167842344
70-74	21.009593275812186	29.273974472142942	28.80642020922221	20.91001204282266
75-79	21.016278590341877	29.247958322939326	28.82471611184636	20.911046974872434
80-84	21.031164621349237	29.254958291872384	28.833998627025853	20.879878459752522
85-89	21.02016659961878	29.214264168955456	28.82124461631051	20.944324615115256
90-94	21.037065261480997	29.220184082327016	28.766768428965705	20.97598222722628
95-99	21.15987558907823	29.29268176147208	28.63912136858663	20.908321280863067
100-101	21.309032877700023	29.302156476310298	28.512817146237644	20.87599349975203
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	38.2485018454599
2	39.58870142628631
3	40.472417611945545
4	41.43413615771652
5	40.66122105035767
6	41.40138096794892
7	40.60841893886227
8	40.124522297042745
9	40.41727013502261
10-14	40.39643713144765
15-19	40.731926801140474
20-24	41.22379146991956
25-29	41.32660321910175
30-34	41.68605162603479
35-39	41.67125741592438
40-44	42.04699647340734
45-49	42.188622965913204
50-54	42.025788596506686
55-59	41.825138860335166
60-64	41.767909447697136
65-69	41.85868447861936
70-74	41.919605318634844
75-79	41.92732556521431
80-84	41.91104308110176
85-89	41.96449121473404
90-94	42.01304748870727
95-99	42.068196869941296
100-101	42.185026377452054
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	456.0
1	434.5
2	481.0
3	625.0
4	772.5
5	886.5
6	1034.5
7	1268.0
8	1527.5
9	1806.0
10	2149.0
11	2682.0
12	3439.5
13	4453.0
14	5887.5
15	7975.0
16	11101.5
17	15903.0
18	22813.5
19	32482.5
20	46019.0
21	64155.5
22	87496.5
23	115890.0
24	150469.5
25	190608.0
26	234693.0
27	281766.5
28	330130.5
29	379711.5
30	429216.0
31	475890.5
32	519919.5
33	559137.0
34	592616.5
35	621943.5
36	645782.0
37	661598.5
38	667385.0
39	664094.5
40	652832.0
41	636384.0
42	615041.5
43	590103.0
44	566911.5
45	547365.5
46	530960.0
47	517306.0
48	502972.5
49	484880.5
50	462544.5
51	435857.0
52	407633.0
53	380050.5
54	354380.0
55	330828.5
56	307763.0
57	283929.0
58	256283.5
59	224910.0
60	191998.0
61	159252.5
62	130216.5
63	106163.5
64	86110.0
65	69431.0
66	55751.0
67	44381.0
68	35009.0
69	27526.5
70	21545.5
71	16800.0
72	13211.5
73	10524.0
74	8426.0
75	6869.5
76	5652.5
77	4600.0
78	3788.0
79	3060.0
80	2353.0
81	1745.0
82	1289.5
83	938.5
84	664.5
85	481.0
86	344.0
87	263.0
88	197.0
89	148.0
90	120.5
91	95.0
92	79.5
93	67.5
94	64.5
95	55.0
96	42.5
97	31.0
98	23.0
99	23.5
100	13.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.073694856124691
2	0.08057534734492773
3	0.08615111008417953
4	0.06366963471951628
5	0.0592369033418111
6	0.006205823928787251
7	0.01238376904387824
8	0.011564131921208228
9	0.02060801908427464
10-14	0.020804285932696308
15-19	0.02367580374341098
20-24	0.018797011346565657
25-29	0.0033889485929172423
30-34	0.005189919957695573
35-39	0.008923450687898576
40-44	0.02310596079145945
45-49	0.02082101322091406
50-54	0.02258183909396978
55-59	0.02580351480470947
60-64	0.021327292477638125
65-69	0.024668289510997803
70-74	0.028338256545973335
75-79	0.029905045875703088
80-84	0.025869308805032642
85-89	0.02119124386680038
90-94	0.024639295544753692
95-99	0.03696396150359586
100-101	0.032961121433087
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.7934766E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	5.192380648124113
#Duplication Level	Relative count
1	100.0
2	3.875111883325436
3	0.36276522929500343
4	0.16848312536197546
5	0.13689253935660506
6	0.10635497288474702
7	0.08529458221450008
8	0.07055230874532722
9	0.05581003527615437
10++	0.4406886747749171
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	2654975	3.749728	8.796886	40-44
CCCAG	3483685	3.719134	4.2561154	25-29
AAAAA	13776865	3.6407256	4.9242377	60-64
CTGGG	3363425	3.4940667	3.7450967	85-89
TTTTT	12070290	3.346834	4.2776804	10-14
CCTCC	3005215	3.2996583	3.8676074	70-74
GGAGG	3256405	3.289254	3.5523825	40-44
CCAGG	3131055	3.2815497	3.6932802	25-29
CCTGG	2948310	3.1198757	3.3976252	70-74
GGGAG	2910450	2.939809	5.4597244	5
GGAAG	2804435	2.025928	5.2582874	5
AGAGC	1902430	1.3999159	5.2870717	8
GAGCG	447495	0.46042815	5.9459968	9
>>END_MODULE