FastQCFastQC Report
Thu 31 Jul 2014
ZL1_CGATGT_L007_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename ZL1_CGATGT_L007_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 17934766
Filtered Sequences 0
Sequence length 101
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC 91819 0.5119609589553608 TruSeq Adapter, Index 2 (100% over 50bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CCCAG 3495735 3.7101305 4.2631497 25-29
CTGGG 3366480 3.518276 3.8398135 1
AAAAA 12636270 3.38564 5.275576 65-69
CCAGG 3136440 3.2966669 3.673093 25-29
GGAGG 3187400 3.2858624 3.6434844 70-74
CCTCC 3016825 3.24595 3.8054466 70-74
TTTTT 11737170 3.207949 3.844085 10-14
CCTGG 2964570 3.1284425 3.3790073 70-74
GGAAG 2796380 2.0583124 9.188917 5
GAAGA 2954230 1.5526075 6.683776 6
AAGAG 2949005 1.5498613 6.614726 7
AGAGC 1948750 1.4483844 8.512444 8
GAGCA 1881490 1.3983942 8.440017 9
GATCG 568775 0.4244208 7.5034513 1
CGGAA 534980 0.39761728 7.532784 4
TCGGA 473120 0.3530429 7.4982347 3
ATCGG 462255 0.34493542 7.486082 2
Produced by FastQC (version 0.10.1)