##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL1_CGATGT_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	17934766	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.20897239473322	34.0	33.0	34.0	31.0	34.0
2	33.3553832260761	34.0	34.0	34.0	31.0	34.0
3	33.38277795205134	34.0	34.0	34.0	31.0	34.0
4	36.6311804123901	37.0	37.0	37.0	35.0	37.0
5	36.57647532172987	37.0	37.0	37.0	35.0	37.0
6	36.56560693348327	37.0	37.0	37.0	35.0	37.0
7	36.566081486650006	37.0	37.0	37.0	35.0	37.0
8	36.56420764006623	37.0	37.0	37.0	35.0	37.0
9	38.428819255294435	39.0	39.0	39.0	37.0	39.0
10-14	38.73322069549165	39.4	39.2	39.4	37.2	39.4
15-19	40.00984540305683	41.0	40.0	41.0	38.0	41.0
20-24	39.94918792918737	41.0	40.0	41.0	38.0	41.0
25-29	39.7820162805581	41.0	40.0	41.0	38.0	41.0
30-34	39.61888226475885	41.0	40.0	41.0	37.4	41.0
35-39	39.40636013873836	41.0	39.8	41.0	37.0	41.0
40-44	39.21425585368663	41.0	39.0	41.0	36.0	41.0
45-49	39.07115569837934	40.2	39.0	41.0	35.6	41.0
50-54	39.031982207072005	40.6	39.0	41.0	35.2	41.0
55-59	38.718425888578636	40.0	38.4	41.0	35.0	41.0
60-64	38.22145688435522	40.0	37.4	41.0	34.4	41.0
65-69	37.52346128184778	39.0	35.8	41.0	33.8	41.0
70-74	36.94981071958229	38.0	35.0	40.2	33.4	41.0
75-79	35.766413947079094	36.2	34.8	39.0	32.4	40.0
80-84	35.37514011612976	35.6	35.0	37.6	33.0	39.2
85-89	34.64641145582831	35.0	35.0	36.4	32.8	37.8
90-94	34.130578085044434	35.0	35.0	35.6	32.0	36.6
95-99	33.831617028067164	35.0	34.6	35.0	32.0	36.0
100-101	33.511466026375814	35.0	34.0	35.0	31.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	12.0
11	21.0
12	36.0
13	148.0
14	650.0
15	1982.0
16	4838.0
17	9186.0
18	13202.0
19	16222.0
20	18805.0
21	21345.0
22	24808.0
23	30020.0
24	37046.0
25	44732.0
26	54022.0
27	66943.0
28	86205.0
29	117770.0
30	145533.0
31	147654.0
32	183234.0
33	249553.0
34	381289.0
35	651958.0
36	1375451.0
37	3572106.0
38	7132372.0
39	3524857.0
40	22762.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.45587894434489	30.906582017634026	29.48105965627974	20.15647938174134
2	20.73634620452708	30.684315016372878	29.706041018014794	18.873297761085247
3	20.73812646977235	29.28963872350925	30.12424498831152	19.847989818406884
4	20.84092031280799	29.04442115493832	29.690138975899266	20.424519556354426
5	21.7139381690288	29.43793077645953	29.535054987614558	19.313076066897107
6	21.456365809289064	29.65748758584305	29.006729165019497	19.879417439848392
7	21.035239601118853	30.170385272938603	29.334807044597067	19.45956808134547
8	20.467504287482758	30.16683908783644	29.457005460790516	19.90865116389029
9	20.81937394666872	29.39931861949021	29.86831832653964	19.912989107301428
10-14	20.403127893299907	29.727796114304322	29.714680797144982	20.154395195250785
15-19	20.42589025952686	29.406357971473646	29.804229941999633	20.36352182699986
20-24	20.598296783234684	29.3632881515962	29.568320163258367	20.470094901910745
25-29	20.80113163876006	29.20814418019817	29.34501297644357	20.645711204598204
30-34	20.80480081568972	29.165962815773383	29.223406193568906	20.805830174967994
35-39	21.105892029668254	29.076340901509596	29.269974197249244	20.547792871572906
40-44	20.924419883313035	29.035633687344493	29.223645426201607	20.816301003140868
45-49	21.168132976837033	28.95547161576285	29.16483773186228	20.711557675537836
50-54	21.012478587110657	28.884332510778098	29.046331529815646	21.0568573722956
55-59	21.06278801592732	28.85591962836276	29.213675149990976	20.86761720571894
60-64	20.997736960184948	28.969908795004518	29.078430940465566	20.953923304344965
65-69	20.919709571508196	29.402498803015238	28.81206482508954	20.86572680038703
70-74	20.98600551227195	29.37104969225667	28.779106433759242	20.863838361712133
75-79	20.97618735427693	29.271424472682195	28.848583589948994	20.90380458309188
80-84	20.99384208276929	29.252163246833195	28.843783649310566	20.91021102108694
85-89	20.967597234305877	29.226750478932118	28.834172693227284	20.971479593534724
90-94	20.973560662274046	29.22344050506303	28.772913282780888	21.030085549882035
95-99	21.02266739116572	29.313920871847067	28.658275030989017	21.005136705998197
100-101	21.118328651587504	29.33914591328725	28.507686477089557	21.03483895803569
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	39.61235832608623
2	39.60964396561233
3	40.58611628817923
4	41.26543986916242
5	41.02701423592591
6	41.33578324913746
7	40.49480768246433
8	40.376155451373045
9	40.73236305397015
10-14	40.557523088550695
15-19	40.789412086526724
20-24	41.068391685145436
25-29	41.44684284335826
30-34	41.61063099065771
35-39	41.653684901241164
40-44	41.7407208864539
45-49	41.87969065237487
50-54	42.06933595940626
55-59	41.93040522164626
60-64	41.95166026452991
65-69	41.78543637189523
70-74	41.849843873984085
75-79	41.87999193736881
80-84	41.90405310385623
85-89	41.9390768278406
90-94	42.003646212156085
95-99	42.02780409716391
100-101	42.153167609623196
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	269.0
1	253.0
2	258.0
3	357.0
4	481.5
5	581.0
6	724.5
7	876.0
8	1053.5
9	1270.5
10	1564.5
11	2005.0
12	2716.5
13	3708.0
14	5069.0
15	7058.0
16	10036.0
17	14627.0
18	21405.0
19	31006.5
20	44613.5
21	62909.0
22	85994.5
23	114443.5
24	148491.0
25	188637.0
26	233237.5
27	280307.0
28	329334.0
29	378780.5
30	427983.5
31	475710.0
32	519724.0
33	558995.0
34	594541.0
35	624789.5
36	648336.0
37	665931.0
38	673813.0
39	670854.5
40	660890.5
41	645033.5
42	622283.5
43	595746.0
44	570530.0
45	549289.0
46	530907.5
47	515215.5
48	500465.5
49	481929.5
50	459711.0
51	434325.0
52	406296.0
53	378224.0
54	351753.0
55	328810.0
56	307418.0
57	283704.0
58	256062.5
59	224695.0
60	192022.0
61	159658.5
62	130753.5
63	106006.5
64	85496.5
65	68924.5
66	55030.0
67	43681.0
68	34146.5
69	26670.5
70	20799.5
71	16154.5
72	12572.0
73	10024.5
74	8109.5
75	6455.5
76	5207.0
77	4224.5
78	3441.0
79	2676.5
80	2035.0
81	1522.5
82	1098.5
83	761.0
84	476.0
85	297.0
86	180.0
87	123.5
88	83.5
89	55.5
90	53.5
91	37.5
92	20.0
93	21.5
94	20.0
95	10.5
96	6.5
97	4.5
98	4.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.903033917476258E-5
2	0.011486071242858702
3	0.002458911368010043
4	0.006027399521131193
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	6.133339013176976E-5
15-19	2.899396624410934E-4
20-24	0.0022068868921958614
25-29	0.011807235176639605
30-34	0.00713809145879015
35-39	0.030749216354425814
40-44	0.025105429309755144
45-49	0.02749185576215491
50-54	0.028033819900410185
55-59	0.024649331917684345
60-64	0.0544250200978368
65-69	0.09382335961339
70-74	0.015483893126902242
75-79	0.017568113238834563
80-84	0.0029016269295066353
85-89	0.013219018302218161
90-94	0.015845202552405756
95-99	0.009139790282181546
100-101	0.00120994051441764
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.7934766E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	5.693267390710048
#Duplication Level	Relative count
1	100.0
2	3.9202871433766755
3	0.3526044768646136
4	0.18816113339412116
5	0.1117371436402064
6	0.10172296567245205
7	0.09065466370809198
8	0.09065466370809198
9	0.07326161776409759
10++	0.48331585244372294
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC	91819	0.5119609589553608	TruSeq Adapter, Index 2 (100% over 50bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCCAG	3495735	3.7101305	4.2631497	25-29
CTGGG	3366480	3.518276	3.8398135	1
AAAAA	12636270	3.38564	5.275576	65-69
CCAGG	3136440	3.2966669	3.673093	25-29
GGAGG	3187400	3.2858624	3.6434844	70-74
CCTCC	3016825	3.24595	3.8054466	70-74
TTTTT	11737170	3.207949	3.844085	10-14
CCTGG	2964570	3.1284425	3.3790073	70-74
GGAAG	2796380	2.0583124	9.188917	5
GAAGA	2954230	1.5526075	6.683776	6
AAGAG	2949005	1.5498613	6.614726	7
AGAGC	1948750	1.4483844	8.512444	8
GAGCA	1881490	1.3983942	8.440017	9
GATCG	568775	0.4244208	7.5034513	1
CGGAA	534980	0.39761728	7.532784	4
TCGGA	473120	0.3530429	7.4982347	3
ATCGG	462255	0.34493542	7.486082	2
>>END_MODULE