##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Undetermined_Undetermined_L008_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5189579	
Filtered Sequences	0	
Sequence length	101	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.543451597904184	34.0	31.0	34.0	15.0	34.0
2	29.496464549436478	34.0	31.0	34.0	10.0	34.0
3	29.489368212720144	34.0	31.0	34.0	10.0	34.0
4	32.416423374612855	37.0	35.0	37.0	10.0	37.0
5	32.40141695501697	37.0	35.0	37.0	10.0	37.0
6	32.47424925991106	37.0	35.0	37.0	10.0	37.0
7	32.491445452511655	37.0	35.0	37.0	10.0	37.0
8	32.48515688844895	37.0	35.0	37.0	10.0	37.0
9	34.03257239171039	39.0	37.0	39.0	10.0	39.0
10-14	34.224369260011265	39.4	37.2	39.4	8.0	39.4
15-19	35.279884630333214	40.6	38.0	41.0	8.2	41.0
20-24	35.236144087988635	40.0	38.0	41.0	8.2	41.0
25-29	35.057562588410356	40.0	38.0	41.0	8.0	41.0
30-34	34.8246964541825	40.0	37.4	41.0	8.0	41.0
35-39	34.642036011013616	40.0	37.0	41.0	8.0	41.0
40-44	34.502366338386985	40.0	36.6	41.0	8.0	41.0
45-49	34.2818077921157	40.0	35.8	41.0	8.0	41.0
50-54	33.63796130668788	39.2	34.8	40.2	7.2	40.6
55-59	34.04713318748978	39.4	35.0	41.0	7.0	41.0
60-64	33.528677682717614	38.6	35.0	40.8	7.0	41.0
65-69	32.80870247856329	36.8	34.2	39.8	7.0	41.0
70-74	32.02109882131093	35.4	34.0	38.6	7.0	40.8
75-79	31.280412264655762	35.0	33.2	36.8	7.0	39.2
80-84	30.676770581968206	35.0	33.0	35.8	7.0	37.8
85-89	30.21430150692378	35.0	32.4	35.0	7.0	36.4
90-94	29.93298227081619	35.0	32.0	35.0	7.0	36.0
95-99	29.65881278616242	35.0	31.4	35.0	7.0	35.2
100-101	29.066103049977656	34.5	30.0	35.0	7.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	307111.0
3	15003.0
4	8734.0
5	7176.0
6	11871.0
7	57226.0
8	113991.0
9	59621.0
10	24171.0
11	14487.0
12	11538.0
13	10716.0
14	9504.0
15	8760.0
16	8182.0
17	8445.0
18	8600.0
19	9054.0
20	9715.0
21	10399.0
22	10875.0
23	11913.0
24	13210.0
25	14517.0
26	16878.0
27	19280.0
28	22523.0
29	26746.0
30	32493.0
31	41965.0
32	56717.0
33	82133.0
34	130326.0
35	246454.0
36	618077.0
37	1356601.0
38	1393628.0
39	377748.0
40	3191.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.785243773350324	30.30473683753693	30.33736890855952	20.57265048055323
2	21.60183191078931	31.605333218825145	27.758456814998812	19.034378055386732
3	21.28899610237645	29.856708719774222	28.184609020863217	20.669686156986113
4	21.49914416373382	28.430849772586175	28.555738854759888	21.514267208920117
5	22.0341326068638	28.86515859768347	29.05728675696015	20.04342203849258
6	21.953627746984267	28.773575180383272	27.963345228306792	21.309451844325668
7	22.070535002919563	28.888515210835035	28.674071117885795	20.366878668359607
8	21.43669573592451	29.161446265182988	28.697410577745053	20.704447421147446
9	21.384952323636227	28.636878589803427	29.10565027763518	20.87251880892516
10-14	21.40528398073021	28.89998968256698	28.86657789926482	20.828148437437996
15-19	21.37991866405699	28.657942087346385	28.95649184295031	21.005647405646314
20-24	21.57887443741122	28.516099834685015	28.771046055325854	21.13397967257791
25-29	21.63625599392651	28.619615244160258	28.511291246263255	21.23283751564998
30-34	21.665105405448102	28.47005791936055	28.566526248720564	21.298310426470785
35-39	21.693750920229967	28.42794345548068	28.520704280200743	21.357601344088607
40-44	21.734020076732197	28.387426759067058	28.491996764164927	21.38655640003582
45-49	21.703338785374925	28.29185787189468	28.522319284958886	21.482484057771504
50-54	21.73657794001243	28.053963476027764	28.663134739164498	21.546323844795307
55-59	21.686511411512317	27.956663651166995	28.76567086251906	21.591154074801626
60-64	21.638469432828288	28.082179728772232	28.670856550612143	21.608494287787334
65-69	21.621708120669332	28.027568431821408	28.711361150231347	21.639362297277916
70-74	21.588781774343087	28.018716917360507	28.71982092380715	21.672680384489258
75-79	21.596260573214764	28.05267613582918	28.648003299494224	21.703059991461835
80-84	21.530708516825552	28.075274238735137	28.68676978024117	21.707247464198144
85-89	21.549733452631074	28.06417369407232	28.70428212073656	21.681810732560045
90-94	21.62157722939879	27.945154452689746	28.716334586182246	21.716933731729217
95-99	21.698850828367895	27.927339270662287	28.775256181313125	21.598553719656696
100-101	21.73836221036765	28.015897903056164	28.62889064029671	21.616849246279475
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	39.35789425390355
2	40.63620996617605
3	41.95868225936256
4	43.013411372653934
5	42.07755464535638
6	43.26307959130993
7	42.43741367127917
8	42.14114315707195
9	42.25747113256139
10-14	42.233432418168206
15-19	42.38556606970331
20-24	42.71285410998913
25-29	42.86909350957649
30-34	42.96341583191889
35-39	43.051352264318574
40-44	43.120576476768015
45-49	43.18582284314643
50-54	43.282901784807734
55-59	43.277665486313936
60-64	43.246963720615625
65-69	43.26107041794725
70-74	43.261462158832344
75-79	43.2993205646766
80-84	43.237955981023696
85-89	43.23154418519112
90-94	43.33851096112801
95-99	43.297404548024595
100-101	43.355211456647126
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	282572.0
1	147355.0
2	11014.0
3	8861.5
4	7101.0
5	5769.0
6	4971.0
7	4508.0
8	4119.0
9	3848.0
10	3614.5
11	3480.0
12	3317.5
13	3224.5
14	3254.0
15	3329.5
16	3554.0
17	4139.5
18	5053.5
19	6328.5
20	8331.0
21	10925.5
22	13924.5
23	17795.5
24	22500.5
25	27606.0
26	33148.0
27	39114.0
28	45221.5
29	51599.5
30	57633.0
31	63554.5
32	69630.5
33	75463.5
34	86273.5
35	96584.5
36	109304.5
37	131821.5
38	160952.5
39	180736.0
40	192804.0
41	226001.5
42	258123.0
43	269763.5
44	277177.0
45	289902.0
46	303087.5
47	306717.5
48	272333.5
49	201501.5
50	147804.0
51	120830.5
52	98908.5
53	88932.5
54	86097.5
55	70206.5
56	51032.5
57	38851.5
58	32568.5
59	28872.5
60	25051.0
61	21283.5
62	17601.0
63	14505.5
64	12179.0
65	10195.0
66	8475.5
67	6967.5
68	5690.0
69	4669.5
70	3750.5
71	2961.5
72	2422.5
73	2008.5
74	1657.0
75	1376.5
76	1150.5
77	995.5
78	881.0
79	734.5
80	614.5
81	535.0
82	426.5
83	348.0
84	285.5
85	225.0
86	186.5
87	145.0
88	109.5
89	85.5
90	72.0
91	62.5
92	49.0
93	36.0
94	26.0
95	16.5
96	10.0
97	6.5
98	7.5
99	6.5
100	4.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	6.759334427705986
2	6.660944943703526
3	6.644700851456352
4	6.730488157131822
5	6.746231245347648
6	6.5390853477709845
7	6.3775886252044725
8	6.567276459227232
9	6.5698970956989
10-14	6.766756995124268
15-19	6.5916483784137405
20-24	6.412658907398846
25-29	6.392522399215814
30-34	6.5737201418458024
35-39	6.242267436337321
40-44	6.477974417577996
45-49	6.608516798761518
50-54	6.816167554246694
55-59	6.810841496005746
60-64	6.697410329431347
65-69	6.6774665151065244
70-74	6.664806528622071
75-79	6.791957497901083
80-84	6.6497802615587895
85-89	6.665272847758942
90-94	6.616031859231741
95-99	6.67431018970903
100-101	6.811140171485971
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5189579.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	51.00006162469489
#Duplication Level	Relative count
1	100.0
2	2.505454366500621
3	0.44564498225622123
4	0.168962330695917
5	0.09678424768018548
6	0.05468036592100874
7	0.04319748907759691
8	0.03390182687102542
9	0.02405936100524385
10++	5.988046872009668
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	275347	5.3057675776782665	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	3485465	4.053377	5.3197017	2
>>END_MODULE