##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Undetermined_Undetermined_L008_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5189579	
Filtered Sequences	0	
Sequence length	101	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07260319189668	34.0	33.0	34.0	31.0	34.0
2	32.97219947899435	34.0	34.0	34.0	31.0	34.0
3	33.175580138581566	34.0	34.0	34.0	31.0	34.0
4	36.53511257849625	37.0	37.0	37.0	35.0	37.0
5	36.52181092146396	37.0	37.0	37.0	35.0	37.0
6	36.517607497640945	37.0	37.0	37.0	35.0	37.0
7	36.53205664659889	37.0	37.0	37.0	35.0	37.0
8	36.54247059347203	37.0	37.0	37.0	35.0	37.0
9	38.38419821723496	39.0	39.0	39.0	37.0	39.0
10-14	38.68378976406372	39.4	39.2	39.4	37.2	39.4
15-19	39.93123288035503	41.0	40.0	41.0	38.0	41.0
20-24	39.856861953541895	41.0	40.0	41.0	38.0	41.0
25-29	39.68302881601764	41.0	40.0	41.0	37.6	41.0
30-34	39.5033974046835	41.0	39.8	41.0	37.0	41.0
35-39	39.25517272210327	40.4	39.0	41.0	36.4	41.0
40-44	38.9835037100312	40.0	38.6	41.0	35.4	41.0
45-49	38.71832693942996	40.0	38.0	41.0	35.0	41.0
50-54	38.52401957846676	40.0	37.8	41.0	35.0	41.0
55-59	37.99964151234618	39.8	36.8	41.0	34.0	41.0
60-64	36.898031111964954	38.8	35.4	40.8	32.6	41.0
65-69	34.33872832459049	36.8	35.0	39.8	27.4	41.0
70-74	33.76954589187292	35.6	35.0	38.8	27.0	40.8
75-79	32.834805405216876	35.0	33.8	36.8	26.6	39.2
80-84	32.68056221901622	35.0	34.0	36.0	27.0	38.2
85-89	32.24830534422927	35.0	34.0	35.2	26.2	36.8
90-94	31.973285270346594	35.0	34.0	35.0	25.8	36.0
95-99	31.75709405329411	35.0	34.0	35.0	25.0	35.8
100-101	31.44165201069297	35.0	33.5	35.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	7.0
11	45.0
12	140.0
13	591.0
14	1633.0
15	2416.0
16	3214.0
17	4859.0
18	7629.0
19	10354.0
20	12864.0
21	16261.0
22	23401.0
23	40609.0
24	92958.0
25	157157.0
26	20615.0
27	19079.0
28	21690.0
29	26357.0
30	32026.0
31	38116.0
32	50980.0
33	73401.0
34	120305.0
35	242428.0
36	667890.0
37	1494509.0
38	1522426.0
39	480475.0
40	5140.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.766382353788785	30.995216279002747	28.02024972520634	21.218151642002127
2	21.597636994974938	30.67950800188988	28.372782614400382	19.35007238873481
3	20.907958044380866	29.213545067913987	28.81019828390704	21.068298603798112
4	21.19674584333115	29.11844804843525	28.29899019966585	21.385815908567746
5	21.99989633070428	29.67167471581028	28.20513956912497	20.123289384360465
6	21.970182937768172	29.363769199775163	27.497375027916522	21.168672834540143
7	21.785562952216356	30.089030343309158	27.60183436845262	20.52357233602186
8	21.293268683259278	29.97667055458641	27.799056532331427	20.931004229822882
9	21.380578270414613	29.366100795459516	28.260905171691192	20.99241576243468
10-14	21.194520595247795	29.916876903004656	27.82424717457546	21.064355327172088
15-19	21.167969235805423	29.68196979701411	27.935370587924485	21.21469037925598
20-24	21.343076340308663	29.60499924213149	27.753749811207307	21.29817460635254
25-29	21.523470969149937	29.56642102511877	27.53333432778671	21.376773677944577
30-34	21.53465917031421	29.587099617841428	27.48369734508595	21.39454386675842
35-39	21.785840232575516	29.45630868957108	27.4453639321173	21.3124871457361
40-44	21.68663650058663	29.566958023242506	27.382875057575625	21.36353041859524
45-49	21.702628323057883	29.505951291198535	27.351918884618343	21.439501501125243
50-54	21.667289254380837	29.469789100263803	27.387525970410692	21.475395674944668
55-59	21.734207904884816	29.518042387293647	27.32678510293434	21.420964604887196
60-64	21.75642983209922	29.60439063486011	27.165253275866146	21.473926257174522
65-69	21.72976378331395	29.763124585618712	26.94972058390997	21.557391047157374
70-74	21.79106120062754	29.806966662367508	26.850687134285124	21.551285002719826
75-79	21.745305863676023	29.770697906346044	26.885235516402155	21.59876071357578
80-84	21.787428806400243	29.844240967726776	26.83883997035999	21.529490255512997
85-89	21.78351656384593	29.82941928750807	26.86002589985723	21.527038248788777
90-94	21.880020058340545	29.687371842239024	26.8975447938291	21.535063305591333
95-99	21.892874794003188	29.716272481611767	26.896038791650827	21.494813932734218
100-101	21.972906446387334	29.78120327530376	26.735122364337332	21.510767913971577
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	40.98453399579091
2	40.94770938370974
3	41.976256648178975
4	42.58256175189889
5	42.12318571506475
6	43.13885577230831
7	42.30913528823822
8	42.22427291308216
9	42.37299403284929
10-14	42.25887592241988
15-19	42.3826596150614
20-24	42.6412509466612
25-29	42.90024464709452
30-34	42.929203037072625
35-39	43.098327378311616
40-44	43.05016691918187
45-49	43.14212982418313
50-54	43.142684929325505
55-59	43.15517250977201
60-64	43.23035608927374
65-69	43.28715483047132
70-74	43.34234620334737
75-79	43.3440665772518
80-84	43.31691906191323
85-89	43.310554812634706
90-94	43.415083363931885
95-99	43.38768872673741
100-101	43.48367436035891
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	252.0
1	154.0
2	63.5
3	82.0
4	113.0
5	164.5
6	277.5
7	415.0
8	606.0
9	906.0
10	1355.5
11	1967.0
12	2809.0
13	3988.5
14	5460.5
15	7198.0
16	9305.5
17	11636.0
18	14345.5
19	17570.5
20	21275.0
21	25556.0
22	30206.0
23	34876.5
24	40174.5
25	45749.5
26	51407.5
27	57432.5
28	63203.0
29	68659.5
30	73760.5
31	78695.0
32	83555.0
33	89240.0
34	99658.5
35	107522.0
36	116993.0
37	134448.0
38	154476.5
39	170665.5
40	189161.0
41	225640.5
42	242550.5
43	244579.5
44	275368.0
45	302551.5
46	296923.0
47	301278.5
48	290717.0
49	241176.5
50	194407.0
51	148945.0
52	107608.5
53	88665.5
54	78237.0
55	59888.5
56	46052.5
57	37349.5
58	31812.0
59	28012.0
60	23888.0
61	19893.0
62	16436.5
63	13617.5
64	11207.0
65	9177.5
66	7566.5
67	6187.0
68	4953.5
69	3907.0
70	3106.5
71	2452.0
72	1948.0
73	1546.0
74	1242.5
75	1004.0
76	864.5
77	781.0
78	652.0
79	530.0
80	417.0
81	338.0
82	262.5
83	178.5
84	122.5
85	84.5
86	55.0
87	32.5
88	21.5
89	15.5
90	10.5
91	8.5
92	5.5
93	6.5
94	8.0
95	6.5
96	4.5
97	4.0
98	3.5
99	2.0
100	6.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.3725928442364978
2	1.0160747143458073
3	0.0
4	0.05035090515049487
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	4.3548811955651897E-4
15-19	0.00126407170986317
20-24	4.7017301403447177E-4
25-29	0.015392385393882626
30-34	0.008174073465304217
35-39	0.04979594683884762
40-44	0.06467962044705361
45-49	0.1579935482242394
50-54	0.28788462416700855
55-59	0.30886127757184156
60-64	1.4006531165630198
65-69	6.392314289848946
70-74	6.353382422736026
75-79	6.4361791197320635
80-84	6.35238041467333
85-89	6.262477168186475
90-94	6.261956894769306
95-99	6.216974440508565
100-101	6.075309769829113
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5189579.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	48.27858697093961
#Duplication Level	Relative count
1	100.0
2	2.3867077728971706
3	0.39505633918096744
4	0.1817040897061632
5	0.08184868905683028
6	0.05511145063159905
7	0.037650396966141926
8	0.01800671159250266
9	0.02128065915477587
10++	5.954219299920879
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE