##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Undetermined_Undetermined_L007_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4943921	
Filtered Sequences	0	
Sequence length	101	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.969318279964426	34.0	31.0	34.0	26.0	34.0
2	30.89345177643413	34.0	31.0	34.0	26.0	34.0
3	30.887593470850362	34.0	31.0	34.0	26.0	34.0
4	33.951707359401574	37.0	37.0	37.0	28.0	37.0
5	33.93291883102501	37.0	37.0	37.0	28.0	37.0
6	33.98904068248663	37.0	37.0	37.0	29.0	37.0
7	33.98449611148722	37.0	37.0	37.0	29.0	37.0
8	33.997466585732255	37.0	37.0	37.0	29.0	37.0
9	35.61713364756435	39.0	38.0	39.0	29.0	39.0
10-14	35.8652083639686	39.4	38.2	39.4	28.6	39.4
15-19	37.000138634901326	41.0	39.0	41.0	27.8	41.0
20-24	36.92521247811201	41.0	39.0	41.0	27.2	41.0
25-29	36.74839403784971	40.2	38.2	41.0	27.0	41.0
30-34	36.52025742320721	40.0	38.0	41.0	25.2	41.0
35-39	36.287204751046794	40.0	38.0	41.0	24.2	41.0
40-44	36.20152194988553	40.0	38.0	41.0	24.0	41.0
45-49	35.97312036337151	40.0	37.2	41.0	23.2	41.0
50-54	35.3000831526232	39.2	36.2	40.2	23.0	40.6
55-59	35.71751720951852	39.8	35.8	41.0	23.4	41.0
60-64	35.17640103877064	38.8	35.0	41.0	23.0	41.0
65-69	34.43114612875085	37.2	35.0	39.8	22.2	41.0
70-74	33.6240668085109	35.8	34.6	38.8	20.8	40.8
75-79	32.8459079342085	35.0	34.0	37.0	20.0	39.4
80-84	32.222464477082056	35.0	34.0	36.0	19.4	38.0
85-89	31.736852914923197	35.0	33.6	35.2	18.0	36.6
90-94	31.447594247561803	35.0	33.0	35.0	18.0	36.0
95-99	31.16833784358609	35.0	33.0	35.0	17.2	35.4
100-101	30.578079423194666	34.5	32.0	35.0	13.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	78828.0
3	7382.0
4	4305.0
5	4935.0
6	8451.0
7	56078.0
8	115665.0
9	61360.0
10	24195.0
11	14765.0
12	11484.0
13	10239.0
14	9071.0
15	8261.0
16	7912.0
17	7952.0
18	8389.0
19	8716.0
20	9038.0
21	9550.0
22	10218.0
23	11075.0
24	12140.0
25	13317.0
26	15151.0
27	18044.0
28	21128.0
29	24517.0
30	29920.0
31	38307.0
32	51866.0
33	74302.0
34	118995.0
35	228643.0
36	590058.0
37	1358580.0
38	1450006.0
39	407434.0
40	3644.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.80676446498295	30.269839031467562	30.360271951197603	20.563124552351887
2	21.64689313415359	31.59286059489262	27.687397823215143	19.072848447738647
3	21.300875980247145	29.840346700979488	28.142452435034336	20.71632488373903
4	21.478559975812907	28.4541159774419	28.535255909853515	21.532068136891677
5	22.03473398802332	28.878354170295857	29.062722077881286	20.02418976379954
6	21.96172922379293	28.79770429837186	27.92756864852993	21.312997829305285
7	22.057274252929044	28.926505687560038	28.66745378696181	20.348766272549106
8	21.44900485062698	29.148010475191384	28.710003172111502	20.69298150207013
9	21.353465492093434	28.60945054618316	29.142295042972794	20.89478891875061
10-14	21.410677185842285	28.905341882160755	28.84009427721067	20.843886654786285
15-19	21.35565007483502	28.69395673715195	28.915302596191562	21.035090591821476
20-24	21.549128456382697	28.551171232415495	28.73582063586048	21.163879675341327
25-29	21.642295923763623	28.636287820731287	28.479666031858347	21.241750223646743
30-34	21.640566932733165	28.489438720476347	28.53757749423976	21.33241685255073
35-39	21.6817487997304	28.446202785134727	28.491177429843084	21.38087098529179
40-44	21.707927706970015	28.420828909282157	28.460143559806756	21.411099823941075
45-49	21.709192816352072	28.304659188236187	28.48029550282322	21.505852492588517
50-54	21.70101622108272	28.088677433569348	28.63858070628394	21.57172563906399
55-59	21.692167643720794	27.984618798899625	28.70135149937663	21.621862058002954
60-64	21.61545794880976	28.073359583424455	28.670435005247512	21.640747462518274
65-69	21.59963109914219	28.038229379998413	28.694878525857497	21.6672609950019
70-74	21.587451829094096	28.014396208685543	28.71591561939404	21.682236342826325
75-79	21.578471242025515	28.05873264513797	28.62811091690711	21.734685195929405
80-84	21.533894244614267	28.067597536900763	28.665230842785128	21.733277375699842
85-89	21.52810284661465	28.080343356877773	28.662081251328047	21.72947254517953
90-94	21.61396823282619	27.952418556734848	28.70426150663498	21.729351703803978
95-99	21.678330105423058	27.939630009631937	28.75074073968607	21.631299145258932
100-101	21.695679037780884	28.00825918234549	28.614954246405894	21.68110753346773
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	39.369889017334835
2	40.71974158189223
3	42.017200863986176
4	43.01062811270459
5	42.05892375182286
6	43.27472705309822
7	42.40604052547815
8	42.14198635269711
9	42.24825441084404
10-14	42.25456384062857
15-19	42.3907406666565
20-24	42.713008131724024
25-29	42.884046147410366
30-34	42.972983785283894
35-39	43.062619785022186
40-44	43.11902753091108
45-49	43.21504530894059
50-54	43.27274186014671
55-59	43.31402970172375
60-64	43.25620541132803
65-69	43.26689209414409
70-74	43.26968817192042
75-79	43.313156437954916
80-84	43.26717162031411
85-89	43.25757539179418
90-94	43.343319936630174
95-99	43.30962925068199
100-101	43.376786571248616
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	63299.0
1	36360.5
2	7601.5
3	4857.0
4	3680.5
5	3176.5
6	2766.5
7	2486.5
8	2298.0
9	2187.5
10	2107.5
11	2130.0
12	2264.5
13	2400.5
14	2570.0
15	2804.0
16	3237.5
17	3962.0
18	4947.0
19	6319.5
20	8336.5
21	10988.0
22	14233.0
23	18006.0
24	22507.5
25	27756.0
26	33578.5
27	39831.5
28	46023.0
29	52223.0
30	58714.5
31	64696.0
32	70221.0
33	75979.0
34	86718.0
35	97525.0
36	110493.0
37	132888.0
38	161520.5
39	180740.5
40	192547.0
41	224627.5
42	255807.0
43	267365.5
44	274957.0
45	287927.5
46	300111.0
47	303397.0
48	270548.0
49	200626.5
50	147492.5
51	120864.5
52	99145.5
53	89172.5
54	86294.5
55	71057.0
56	52083.0
57	39927.5
58	33773.0
59	29745.5
60	25677.0
61	21769.5
62	18253.0
63	15177.0
64	12638.0
65	10675.0
66	8935.5
67	7447.0
68	6060.0
69	4837.0
70	3922.5
71	3173.0
72	2544.0
73	2112.0
74	1764.0
75	1494.0
76	1229.5
77	998.0
78	863.5
79	740.5
80	645.0
81	553.5
82	439.5
83	353.0
84	287.0
85	218.0
86	169.0
87	156.0
88	137.5
89	99.5
90	69.5
91	54.5
92	46.5
93	35.0
94	26.0
95	22.0
96	14.5
97	9.0
98	6.5
99	5.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.0338512690635633
2	2.0224433197860563
3	2.0198947353729966
4	2.0564446721539444
5	2.117428656323594
6	2.010327430393811
7	2.0247896355949053
8	1.9937211779880788
9	2.040020461492002
10-14	2.1015020264280113
15-19	1.9131980466516354
20-24	1.8963976163858605
25-29	1.894002756112001
30-34	2.0144577552918017
35-39	1.9748454718430977
40-44	2.0520433073263105
45-49	2.048993096774807
50-54	2.081097169635194
55-59	2.095963912044711
60-64	2.0709999209129757
65-69	2.0866514655068316
70-74	2.0672215433863124
75-79	2.09882803548034
80-84	2.018236132818465
85-89	1.9723535226392168
90-94	1.8383424816051874
95-99	1.9772079691402835
100-101	2.0687931704410323
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4943921.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	48.507134062025806
#Duplication Level	Relative count
1	100.0
2	2.305174113635497
3	0.35732377564860257
4	0.1634102632539341
5	0.08442863601453261
6	0.045210172833588434
7	0.032137351773273704
8	0.02560094124311634
9	0.015251624570367182
10++	5.911638623649822
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	63361	1.2815941031420202	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	3391580	3.9402785	5.24107	2
>>END_MODULE