##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename Undetermined_Undetermined_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4943921 Filtered Sequences 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.0900073039193 34.0 33.0 34.0 31.0 34.0 2 33.2226720451237 34.0 34.0 34.0 31.0 34.0 3 33.33279860256667 34.0 34.0 34.0 31.0 34.0 4 36.622103993975635 37.0 37.0 37.0 35.0 37.0 5 36.582173339743896 37.0 37.0 37.0 35.0 37.0 6 36.576276198588126 37.0 37.0 37.0 35.0 37.0 7 36.58190189527705 37.0 37.0 37.0 35.0 37.0 8 36.58329127831937 37.0 37.0 37.0 35.0 37.0 9 38.43696369743772 39.0 39.0 39.0 37.0 39.0 10-14 38.74254471299197 39.4 39.2 39.4 37.2 39.4 15-19 40.01953514224843 41.0 40.0 41.0 38.0 41.0 20-24 39.955749009743485 41.0 40.0 41.0 38.0 41.0 25-29 39.73390727724007 41.0 40.0 41.0 38.0 41.0 30-34 39.63739214279516 41.0 40.0 41.0 37.6 41.0 35-39 39.41909104130102 41.0 39.6 41.0 37.0 41.0 40-44 39.17813334800455 40.2 39.0 41.0 36.0 41.0 45-49 38.91839910063288 40.0 38.6 41.0 35.0 41.0 50-54 38.6896115451683 40.0 38.0 41.0 35.0 41.0 55-59 38.17433765628537 40.0 36.8 41.0 34.6 41.0 60-64 37.40328629846634 38.8 35.4 40.8 33.8 41.0 65-69 36.20010752599 37.2 35.0 40.0 32.8 41.0 70-74 35.55505138532756 36.2 35.0 39.0 33.0 41.0 75-79 34.538986484614135 35.0 34.4 37.2 31.6 39.2 80-84 34.35378999785797 35.0 35.0 36.2 33.0 38.4 85-89 33.93225692724459 35.0 35.0 35.4 32.0 36.8 90-94 33.69481555227116 35.0 35.0 35.0 32.0 36.0 95-99 33.4501330017207 35.0 34.0 35.0 31.8 36.0 100-101 33.09097091154976 35.0 34.0 35.0 30.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 0.0 8 1.0 9 3.0 10 9.0 11 30.0 12 83.0 13 332.0 14 580.0 15 982.0 16 1917.0 17 3317.0 18 6302.0 19 9495.0 20 9896.0 21 11381.0 22 13022.0 23 18205.0 24 25202.0 25 19399.0 26 15338.0 27 16537.0 28 19996.0 29 23706.0 30 27806.0 31 33764.0 32 43627.0 33 63379.0 34 103670.0 35 211202.0 36 618274.0 37 1498033.0 38 1615646.0 39 526898.0 40 5888.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.792096658334984 30.988774982854505 27.965337660606966 21.25379069820354 2 21.59631548020985 30.68818742857885 28.326460400218163 19.389036690993144 3 20.955086793524497 29.258271265718168 28.704749907055337 21.081892033701997 4 21.19706483600552 29.12563247905087 28.24526937832473 21.432033306618887 5 21.985140943797443 29.698370989342266 28.180021484971142 20.136466581889152 6 21.996265717029054 29.37431645853564 27.452400635042512 21.177017189392792 7 21.85255387373706 30.03957789778599 27.564133812008727 20.543734416468222 8 21.299045838313354 29.984560837440565 27.78321093722978 20.9331823870163 9 21.33227856998524 29.39407810116707 28.21448805512871 21.05915527371898 10-14 21.239063280693983 29.909533704701367 27.76577268080425 21.0856303338004 15-19 21.18694689970138 29.694142144010716 27.88855706837697 21.230353887910937 20-24 21.376165405522002 29.609203061699958 27.690959994543483 21.323671538234557 25-29 21.57616110779528 29.582568644060895 27.445224923223314 21.396045324920507 30-34 21.555799832889704 29.613646550440336 27.41121006406434 21.419343552605618 35-39 21.82086315227577 29.45327402831578 27.387214131219334 21.338648688189117 40-44 21.727969697521534 29.592535262850124 27.3127796089572 21.366715430671142 45-49 21.73616967236403 29.50942885216777 27.26505297320903 21.489348502259173 50-54 21.715576096907803 29.477623352564486 27.316814256931593 21.489986293596118 55-59 21.767275139794716 29.538330463064767 27.26414516995404 21.43024922718648 60-64 21.767240074545963 29.623439030858595 27.110789706214515 21.498531188380927 65-69 21.738259426649858 29.729044160605355 26.985849240324782 21.54684717242 70-74 21.78958319736986 29.77278668635808 26.909512129956774 21.52811798631528 75-79 21.741262272954287 29.75267088912867 26.93767424742767 21.568392590489378 80-84 21.77825210381064 29.812480991523323 26.906602197270786 21.502664707395255 85-89 21.789867730321603 29.815265165057113 26.88136335785547 21.51350374676581 90-94 21.862324326044043 29.661425543171127 26.936763443614602 21.53948668717023 95-99 21.883982305551026 29.69527732456462 26.933402387085042 21.48733798279931 100-101 21.938454660391997 29.769147909690126 26.791814629002182 21.5005828009157 >>END_MODULE >>Per base GC content pass #Base %GC 1 41.04588735653853 2 40.98535217120299 3 42.0369788272265 4 42.62909814262441 5 42.12160752568659 6 43.173282906421846 7 42.396288290205284 8 42.232228225329656 9 42.39143384370422 10-14 42.32469361449438 15-19 42.41730078761232 20-24 42.69983694375656 25-29 42.97220643271579 30-34 42.97514338549532 35-39 43.15951184046489 40-44 43.09468512819268 45-49 43.225518174623204 50-54 43.20556239050393 55-59 43.19752436698119 60-64 43.26577126292689 65-69 43.28510659906986 70-74 43.317701183685145 75-79 43.30965486344366 80-84 43.28091681120589 85-89 43.30337147708742 90-94 43.40181101321427 95-99 43.37132028835033 100-101 43.43903746130769 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 199.0 1 126.5 2 52.5 3 58.0 4 83.0 5 103.0 6 139.5 7 200.5 8 291.5 9 437.0 10 645.0 11 912.5 12 1267.5 13 1710.5 14 2277.5 15 2976.5 16 3802.5 17 4950.5 18 6478.0 19 8324.0 20 10702.5 21 13839.5 22 17364.0 23 21624.0 24 26785.5 25 32476.0 26 38745.0 27 44887.0 28 51586.0 29 58384.0 30 64564.5 31 70540.5 32 76587.0 33 82940.0 34 93880.0 35 103054.5 36 112825.0 37 130081.5 38 150054.0 39 166730.5 40 186373.0 41 223264.0 42 239651.5 43 241299.0 44 272167.5 45 299676.5 46 294480.5 47 298491.0 48 288275.5 49 239374.0 50 192948.0 51 148059.5 52 107313.0 53 89086.0 54 78386.0 55 60145.0 56 47077.0 57 38465.5 58 32565.0 59 28564.5 60 24449.5 61 20519.5 62 16971.5 63 13948.0 64 11543.0 65 9417.0 66 7659.0 67 6282.0 68 5122.5 69 4015.0 70 3156.5 71 2477.5 72 1960.5 73 1586.5 74 1264.0 75 1048.5 76 877.5 77 740.5 78 615.5 79 514.0 80 412.5 81 310.0 82 254.5 83 178.5 84 108.0 85 77.5 86 47.0 87 22.5 88 20.0 89 12.5 90 9.0 91 9.0 92 6.5 93 7.5 94 4.5 95 2.0 96 2.0 97 2.0 98 1.5 99 3.5 100 8.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.43174233568861636 2 0.3116756922289009 3 0.0024069963901122205 4 0.005643294057489996 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 4.854446501066663E-5 15-19 3.3576588299044424E-4 20-24 0.0021319110883851093 25-29 0.10086730754799683 30-34 0.00727357900743155 35-39 0.040696443167275526 40-44 0.03541723259736553 45-49 0.10361411519318371 50-54 0.27061516557404536 55-59 0.31745248356517025 60-64 0.43775780397785485 65-69 1.537213074399854 70-74 1.6279062711560317 75-79 1.8199239025057237 80-84 1.7572570435490371 85-89 1.5327065298980302 90-94 1.3284880563423243 95-99 1.4869210086488032 100-101 1.6423603856129578 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4943921.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Duplicate Percentage 48.515953187125056 #Duplication Level Relative count 1 100.0 2 2.470200410426582 3 0.376588219883858 4 0.16537134873160722 5 0.08077544426494346 6 0.0452997423918264 7 0.034929921844299876 8 0.02292276121032179 9 0.015281840806881195 10++ 5.944636073876785 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content pass >>END_MODULE