##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename lane8_Undetermined_L008_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 101558750 Sequences flagged as poor quality 0 Sequence length 93 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06429226432976 33.0 33.0 33.0 33.0 33.0 2 32.06237040136867 33.0 33.0 33.0 33.0 33.0 3 32.091736526887146 33.0 33.0 33.0 33.0 33.0 4 35.865782633204915 37.0 37.0 37.0 33.0 37.0 5 35.835029546937115 37.0 37.0 37.0 33.0 37.0 6 35.91163613179564 37.0 37.0 37.0 33.0 37.0 7 35.90574709712359 37.0 37.0 37.0 33.0 37.0 8 35.94396420790922 37.0 37.0 37.0 33.0 37.0 9 35.952260469925044 37.0 37.0 37.0 33.0 37.0 10-11 35.957812113678045 37.0 37.0 37.0 33.0 37.0 12-13 35.93177452952109 37.0 37.0 37.0 33.0 37.0 14-15 37.893695555528204 40.0 37.0 40.0 33.0 40.0 16-17 37.86970548081776 40.0 37.0 40.0 33.0 40.0 18-19 37.871450067079394 40.0 37.0 40.0 33.0 40.0 20-21 37.78427105493125 40.0 37.0 40.0 33.0 40.0 22-23 37.745141122749146 40.0 37.0 40.0 33.0 40.0 24-25 37.64049643679151 40.0 37.0 40.0 33.0 40.0 26-27 37.543592324639675 40.0 37.0 40.0 33.0 40.0 28-29 37.43201418390833 40.0 37.0 40.0 33.0 40.0 30-31 37.32929790884586 40.0 37.0 40.0 33.0 40.0 32-33 37.22941490024247 40.0 37.0 40.0 33.0 40.0 34-35 37.14018546407867 40.0 37.0 40.0 33.0 40.0 36-37 37.01600274717836 40.0 37.0 40.0 33.0 40.0 38-39 36.89356297709454 40.0 37.0 40.0 33.0 40.0 40-41 36.85184730020801 40.0 37.0 40.0 33.0 40.0 42-43 36.86404518074514 40.0 37.0 40.0 33.0 40.0 44-45 36.777366455376814 40.0 37.0 40.0 33.0 40.0 46-47 36.62682952970572 40.0 37.0 40.0 33.0 40.0 48-49 36.462837569387176 38.5 37.0 40.0 33.0 40.0 50-51 35.458966942779426 37.0 35.0 38.5 30.0 40.0 52-53 35.621251615444265 37.0 37.0 38.5 30.0 40.0 54-55 36.31154864548845 37.0 37.0 40.0 33.0 40.0 56-57 36.281300596945115 37.0 37.0 40.0 33.0 40.0 58-59 36.120952670252436 37.0 37.0 40.0 30.0 40.0 60-61 36.27601116595074 37.0 37.0 40.0 33.0 40.0 62-63 36.21370735657932 37.0 37.0 40.0 33.0 40.0 64-65 36.05781895208438 37.0 37.0 40.0 33.0 40.0 66-67 35.847994476103736 37.0 37.0 40.0 27.0 40.0 68-69 35.65117646682339 37.0 37.0 40.0 27.0 40.0 70-71 35.40680253055497 37.0 33.0 40.0 27.0 40.0 72-73 35.17445917756963 37.0 33.0 38.5 27.0 40.0 74-75 34.90336396420791 37.0 33.0 37.0 27.0 40.0 76-77 34.63001984073258 37.0 33.0 37.0 27.0 40.0 78-79 34.36361853114577 37.0 33.0 37.0 27.0 40.0 80-81 34.10786559503735 37.0 33.0 37.0 27.0 37.0 82-83 33.85740065725504 37.0 33.0 37.0 27.0 37.0 84-85 33.64166861545657 37.0 33.0 37.0 27.0 37.0 86-87 33.47768767339102 37.0 33.0 37.0 27.0 37.0 88-89 33.27932256944872 37.0 33.0 37.0 27.0 37.0 90-91 33.006265491648925 37.0 33.0 37.0 27.0 37.0 92-93 27.50289985353305 33.0 18.5 37.0 8.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2304 1 0.12951091070554455 2304 2 0.14304230591489642 2304 3 0.21639642823399896 2304 4 0.0796937640541131 2304 5 0.14044351715239856 2304 6 0.18225119955490499 2304 7 0.25041962920534644 2304 8 0.25932575608798913 2304 9 0.346930254183043 2304 10-11 0.34880222551941387 2304 12-13 0.41275922119979924 2304 14-15 0.6124858197052347 2304 16-17 0.5867122163733853 2304 18-19 0.5714770512045462 2304 20-21 0.5993129102594636 2304 22-23 0.5980074533877726 2304 24-25 0.5689663139701153 2304 26-27 0.5607647579945052 2304 28-29 0.5863703085765124 2304 30-31 0.522453976270775 2304 32-33 0.49553739897137206 2304 34-35 0.508081528900874 2304 36-37 0.461385695922516 2304 38-39 0.48931733291486523 2304 40-41 0.4538612908759916 2304 42-43 0.4287734793811637 2304 44-45 0.39874954766994364 2304 46-47 0.45506545074719185 2304 48-49 0.4150870805686466 2304 50-51 0.36434317150559536 2304 52-53 0.38366532606234927 2304 54-55 0.4650775962204392 2304 56-57 0.48642914708921836 2304 58-59 0.44817073124776385 2304 60-61 0.49442810932583825 2304 62-63 0.5560874135095943 2304 64-65 0.5064063219597656 2304 66-67 0.49717298474119787 2304 68-69 0.468818577402736 2304 70-71 0.5292534054164477 2304 72-73 0.5466395306649474 2304 74-75 0.5206594421780295 2304 76-77 0.5358637271349238 2304 78-79 0.5254524405270828 2304 80-81 0.48175712857459985 2304 82-83 0.4889343883648678 2304 84-85 0.5471456722621539 2304 86-87 0.5053353121786799 2304 88-89 0.5208929158386795 2304 90-91 0.6187283904778909 2304 92-93 4.397903634147408 2305 1 0.39162426124373084 2305 2 0.3638742989352508 2305 3 0.4347630745959066 2305 4 0.4702054004212002 2305 5 0.4408577091728958 2305 6 0.43729153248096964 2305 7 0.5425585208897346 2305 8 0.5098677179342772 2305 9 0.5457408144565505 2305 10-11 0.5234628705835505 2305 12-13 0.5727292068470646 2305 14-15 0.8622407257777525 2305 16-17 0.7862392263929507 2305 18-19 0.7740135231842942 2305 20-21 0.7709176006704084 2305 22-23 0.7860724999009037 2305 24-25 0.811125743397298 2305 26-27 0.7378168398706748 2305 28-29 0.8337693213561508 2305 30-31 0.8353043503024011 2305 32-33 0.8166267040526449 2305 34-35 0.7851671277561962 2305 36-37 0.7989429651374493 2305 38-39 0.7836128978193244 2305 40-41 0.7950882777997634 2305 42-43 0.7557664602562255 2305 44-45 0.7395803245744403 2305 46-47 0.8260529222011073 2305 48-49 0.7863581959727028 2305 50-51 0.7108095122937499 2305 52-53 0.7608853577368038 2305 54-55 0.8663325289625803 2305 56-57 0.8464136497527193 2305 58-59 0.8735367434032923 2305 60-61 0.8908620218615866 2305 62-63 0.8716528471927703 2305 64-65 0.8189113771613776 2305 66-67 0.8686542398627424 2305 68-69 0.8350080630463239 2305 70-71 0.8389616179257047 2305 72-73 0.8355805712236517 2305 74-75 0.8254502274593207 2305 76-77 0.7974857028057372 2305 78-79 0.8279575452207624 2305 80-81 0.8503273043634749 2305 82-83 0.8718589763393325 2305 84-85 0.8630913592742431 2305 86-87 0.8507081535812304 2305 88-89 0.8651527805995087 2305 90-91 0.9319606827136298 2305 92-93 4.72607300156977 2306 1 0.2820190649480132 2306 2 0.30825075547568304 2306 3 0.3587210442488953 2306 4 0.37743918693049494 2306 5 0.2871531077222116 2306 6 0.3319614886216087 2306 7 0.4281616122521612 2306 8 0.441503138972152 2306 9 0.4706996909642527 2306 10-11 0.48985744451670143 2306 12-13 0.5114320868513289 2306 14-15 0.7580456072216535 2306 16-17 0.6730185381643565 2306 18-19 0.6631814519680788 2306 20-21 0.6808853511573645 2306 22-23 0.6611831947530078 2306 24-25 0.6610669179379869 2306 26-27 0.6678840405182953 2306 28-29 0.7274260174561675 2306 30-31 0.7397288384991754 2306 32-33 0.7204932374935282 2306 34-35 0.6948288099509341 2306 36-37 0.642687368118608 2306 38-39 0.6810436477500446 2306 40-41 0.6131293969571487 2306 42-43 0.6623924253745344 2306 44-45 0.6356371664295537 2306 46-47 0.7297002675836879 2306 48-49 0.6832271635946867 2306 50-51 0.6213568479782765 2306 52-53 0.6681609827191792 2306 54-55 0.7249356150183104 2306 56-57 0.7635793855894732 2306 58-59 0.7064105158464358 2306 60-61 0.7603591631113389 2306 62-63 0.7890559717030357 2306 64-65 0.7006389686072296 2306 66-67 0.6865829033897697 2306 68-69 0.6933803018127307 2306 70-71 0.7381750742174376 2306 72-73 0.7397900865604399 2306 74-75 0.7077549055395593 2306 76-77 0.6988793069402206 2306 78-79 0.6536877840731421 2306 80-81 0.6596173605614268 2306 82-83 0.7137170229109557 2306 84-85 0.6607634093596033 2306 86-87 0.6727323511282819 2306 88-89 0.7063687762659683 2306 90-91 0.7844741382590641 2306 92-93 4.659344969158571 2307 1 0.1508359764277607 2307 2 0.1503333772852713 2307 3 0.19967703438890538 2307 4 0.14750417395688942 2307 5 0.13016231883497653 2307 6 0.1750165432962021 2307 7 0.2585688515952427 2307 8 0.25194821142672197 2307 9 0.27666384248849596 2307 10-11 0.310037996645562 2307 12-13 0.28023851866796434 2307 14-15 0.33630555236062065 2307 16-17 0.3941780076264081 2307 18-19 0.3939321886708669 2307 20-21 0.3543293857017247 2307 22-23 0.34971737987294915 2307 24-25 0.326445067972827 2307 26-27 0.34904416689653317 2307 28-29 0.37282040233322533 2307 30-31 0.3739756080344492 2307 32-33 0.3510825929627899 2307 34-35 0.32832650468181157 2307 36-37 0.37371249750955826 2307 38-39 0.2523605570039251 2307 40-41 0.3072438253579932 2307 42-43 0.3390607305541522 2307 44-45 0.26293395141269826 2307 46-47 0.37446020087663356 2307 48-49 0.24385681835882878 2307 50-51 0.1669861482297037 2307 52-53 0.2693509084470378 2307 54-55 0.3775203583607407 2307 56-57 0.43089918724367493 2307 58-59 0.34742923175671336 2307 60-61 0.37264254179891765 2307 62-63 0.39929927633100704 2307 64-65 0.37623853259058393 2307 66-67 0.3777087039531395 2307 68-69 0.36461603269932397 2307 70-71 0.2689874126753722 2307 72-73 0.3494205868240883 2307 74-75 0.315151636322085 2307 76-77 0.3556555645643513 2307 78-79 0.2994638275008157 2307 80-81 0.25523084360182224 2307 82-83 0.3085587642782599 2307 84-85 0.3419164780865884 2307 86-87 0.28287178133466995 2307 88-89 0.29251000836557495 2307 90-91 0.3724787145134698 2307 92-93 4.173363846963962 2308 1 0.1839755636759186 2308 2 0.20125022870903564 2308 3 0.24887628781684867 2308 4 0.2814597552606415 2308 5 0.23685383704783902 2308 6 0.21831157910107635 2308 7 0.3303494574719963 2308 8 0.3483135284502623 2308 9 0.42381960690715204 2308 10-11 0.42740964189150077 2308 12-13 0.4351567964981413 2308 14-15 0.6103015535913556 2308 16-17 0.5420776783485977 2308 18-19 0.5669090887118315 2308 20-21 0.5536917633217442 2308 22-23 0.5312967301402054 2308 24-25 0.5614842832166005 2308 26-27 0.569986703441657 2308 28-29 0.6451354417788409 2308 30-31 0.5957356571349166 2308 32-33 0.5562538862996504 2308 34-35 0.563852233669266 2308 36-37 0.5408066294126641 2308 38-39 0.5534680788334327 2308 40-41 0.5418180753027855 2308 42-43 0.520615379098146 2308 44-45 0.47823973815327037 2308 46-47 0.5601376877649997 2308 48-49 0.5028205870148454 2308 50-51 0.4742656640098275 2308 52-53 0.4976149570514323 2308 54-55 0.599054341194595 2308 56-57 0.6092919437652071 2308 58-59 0.6285917815363575 2308 60-61 0.6863998045070403 2308 62-63 0.6895220330787097 2308 64-65 0.6132020333836792 2308 66-67 0.5574407707153242 2308 68-69 0.5910753755661275 2308 70-71 0.6165702150966439 2308 72-73 0.5999879777166228 2308 74-75 0.5839402502287996 2308 76-77 0.5783449160794092 2308 78-79 0.5644405852086578 2308 80-81 0.5895600144843769 2308 82-83 0.638526587707041 2308 84-85 0.6687283707219578 2308 86-87 0.6002430768395755 2308 88-89 0.5621980765386567 2308 90-91 0.6964283292261442 2308 92-93 4.471758685181026 2309 1 0.3145580455955077 2309 2 0.27514670952634646 2309 3 0.3113354794650576 2309 4 0.25538684561872316 2309 5 0.3118449289911496 2309 6 0.36529400658794486 2309 7 0.37630590793664 2309 8 0.4163870859448764 2309 9 0.3921682243164497 2309 10-11 0.4595409018277863 2309 12-13 0.4604474418260409 2309 14-15 0.6602501743311606 2309 16-17 0.6143211833223319 2309 18-19 0.5417796001031618 2309 20-21 0.5538981397878118 2309 22-23 0.5875291699684766 2309 24-25 0.6351461300306482 2309 26-27 0.5659977826670541 2309 28-29 0.5538438896549209 2309 30-31 0.5148619366347233 2309 32-33 0.5688965445008805 2309 34-35 0.5983978231332685 2309 36-37 0.5598458303498504 2309 38-39 0.5466606012688544 2309 40-41 0.5180513146777201 2309 42-43 0.4264521709707765 2309 44-45 0.46282639836739037 2309 46-47 0.5006761549244914 2309 48-49 0.42862447147329163 2309 50-51 0.5160801502006223 2309 52-53 0.5159029359426484 2309 54-55 0.613249527588664 2309 56-57 0.6005335681050354 2309 58-59 0.6419976966490495 2309 60-61 0.6652356620167055 2309 62-63 0.6140007698146519 2309 64-65 0.6320833701400872 2309 66-67 0.6194983760815731 2309 68-69 0.587518755658472 2309 70-71 0.6428313139815458 2309 72-73 0.6580317127000441 2309 74-75 0.6891245095542757 2309 76-77 0.5611076724606079 2309 78-79 0.6185867562729754 2309 80-81 0.6449055095601608 2309 82-83 0.6310571220132033 2309 84-85 0.5856333396228166 2309 86-87 0.4955670482702459 2309 88-89 0.545833754416023 2309 90-91 0.6592955761617887 2309 92-93 4.518381333595901 2310 1 0.3228474315852061 2310 2 0.3459041890397714 2310 3 0.3701136419838349 2310 4 0.3870146547397013 2310 5 0.4325818591692112 2310 6 0.39493428810344966 2310 7 0.46835872472492923 2310 8 0.3847439902970393 2310 9 0.43182419954302276 2310 10-11 0.4509600326635308 2310 12-13 0.4774962057381842 2310 14-15 0.6697357750670889 2310 16-17 0.6193258391799148 2310 18-19 0.5754932958948302 2310 20-21 0.5974409197729358 2310 22-23 0.6218809708490127 2310 24-25 0.6055264782957508 2310 26-27 0.6375136669682533 2310 28-29 0.642093969148327 2310 30-31 0.607710598444072 2310 32-33 0.5679364467398145 2310 34-35 0.5909898047887978 2310 36-37 0.6036968992385212 2310 38-39 0.5567116150679396 2310 40-41 0.5781049164890959 2310 42-43 0.5762677457825589 2310 44-45 0.5173287835060378 2310 46-47 0.5587512507930299 2310 48-49 0.594279869195951 2310 50-51 0.5575566522185369 2310 52-53 0.5676571553800471 2310 54-55 0.6846325570971246 2310 56-57 0.7138415420097957 2310 58-59 0.734393503994923 2310 60-61 0.6971010446080967 2310 62-63 0.7139048983978142 2310 64-65 0.6448251649838497 2310 66-67 0.6359664872764483 2310 68-69 0.6518516694370149 2310 70-71 0.7064508174683652 2310 72-73 0.6774531739307648 2310 74-75 0.7353498809190029 2310 76-77 0.747144289227684 2310 78-79 0.7619407213787781 2310 80-81 0.71597527020797 2310 82-83 0.7290394734296513 2310 84-85 0.6824841503426526 2310 86-87 0.7019618935115588 2310 88-89 0.7194834615185712 2310 90-91 0.7783407842386296 2310 92-93 4.64025205925422 2311 1 0.28689104215463246 2311 2 0.2593809631938413 2311 3 0.27611157721713653 2311 4 0.2634706483556144 2311 5 0.30896329808756917 2311 6 0.2858609445355711 2311 7 0.3605322583085311 2311 8 0.32066417912968603 2311 9 0.34458845937308524 2311 10-11 0.3176353263237246 2311 12-13 0.35315031609885494 2311 14-15 0.41691039097073457 2311 16-17 0.39298057357224536 2311 18-19 0.3842724388873009 2311 20-21 0.34945576412857804 2311 22-23 0.36763096922521754 2311 24-25 0.41356937122517223 2311 26-27 0.3723201777611109 2311 28-29 0.38972701538053656 2311 30-31 0.33667107892807024 2311 32-33 0.31730417711279557 2311 34-35 0.33151580297580807 2311 36-37 0.3663161041907088 2311 38-39 0.337757306958828 2311 40-41 0.28456031911520085 2311 42-43 0.27446645492813815 2311 44-45 0.16586333493222583 2311 46-47 0.25802016170142394 2311 48-49 0.2572709382477285 2311 50-51 0.1434080304745109 2311 52-53 0.22011098159180875 2311 54-55 0.33896777596096683 2311 56-57 0.33034609287587813 2311 58-59 0.30074613001854544 2311 60-61 0.28546595844706246 2311 62-63 0.3346421509789579 2311 64-65 0.30168153380666496 2311 66-67 0.3427628508873184 2311 68-69 0.26354683284102265 2311 70-71 0.32028488956507317 2311 72-73 0.27079281705669445 2311 74-75 0.33666515405336384 2311 76-77 0.33030687395308433 2311 78-79 0.27559170602523864 2311 80-81 0.2585148585195114 2311 82-83 0.23028462576229458 2311 84-85 0.26690902361622904 2311 86-87 0.24743978507581943 2311 88-89 0.26416658965079876 2311 90-91 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48-49 -0.499276093825074 2303 50-51 -0.666918238612709 2303 52-53 -0.7197056981182541 2303 54-55 -0.816918713415852 2303 56-57 -0.730066326912727 2303 58-59 -0.6729083953308788 2303 60-61 -0.7886956931520572 2303 62-63 -0.7443843448100012 2303 64-65 -0.8615970247184137 2303 66-67 -0.8546104353991879 2303 68-69 -0.76709384581725 2303 70-71 -0.7108712507542378 2303 72-73 -1.3062364947843434 2303 74-75 -0.9077363915435015 2303 76-77 -0.8725029848733925 2303 78-79 -0.8672569991159662 2303 80-81 -0.8595769841473526 2303 82-83 -0.7222262754221163 2303 84-85 -0.6844179740895768 2303 86-87 -0.6134763216917847 2303 88-89 -0.6056071162524432 2303 90-91 -0.4839512497427023 2303 92-93 3.153599487629709 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 270584.0 3 17931.0 4 10841.0 5 9311.0 6 7466.0 7 19573.0 8 36618.0 9 36191.0 10 41499.0 11 60190.0 12 94571.0 13 126262.0 14 148879.0 15 169501.0 16 189517.0 17 213554.0 18 236906.0 19 267100.0 20 299882.0 21 340087.0 22 386948.0 23 442860.0 24 510314.0 25 591481.0 26 696631.0 27 822908.0 28 981768.0 29 1186032.0 30 1476605.0 31 1902715.0 32 2513626.0 33 3458212.0 34 4997704.0 35 7802118.0 36 1.381522E7 37 2.7434678E7 38 2.8865435E7 39 1077032.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.685348597193524 23.536365729646597 11.210874437458095 24.567411235701787 2 15.68371948993344 22.37445767801188 38.805785109613716 23.136037722440967 3 15.481113138737602 27.183709013165963 31.701019182253532 25.634158665842904 4 11.412884816837503 17.046309322388538 42.436787084806824 29.104018775967134 5 11.932061340985626 38.342499819774226 38.051867517507134 11.67357132173301 6 28.54824666852769 41.77927526617718 18.365165061145678 11.307313004149457 7 26.034863433660732 32.617513071060536 26.213168674542608 15.13445482073613 8 23.220577235571586 37.49135680136859 22.941502630069103 16.346563332990723 9 23.684183689199735 15.106920994734141 24.943565549697574 36.265329766368545 10-11 22.594829884681218 27.01911904927872 31.58688568452731 18.799165381512744 12-13 22.045344354197013 25.326834390971698 31.585422132921654 21.042399121909636 14-15 20.01237316600003 26.851499427571586 27.149539351121255 25.986588055307124 16-17 20.0925645885352 31.370105860670094 28.317655301558393 20.219674249236313 18-19 20.21205424057847 30.10966041502085 29.842363465086013 19.835921879314665 20-21 20.469371431060797 29.66585566146519 29.628685611642698 20.23608729583132 22-23 20.596376937091655 29.459351041029393 29.585916362582726 20.358355659296222 24-25 20.14919864910845 29.393958629143835 29.858207563238075 20.59863515850964 26-27 20.379010539203378 29.94032556253648 29.57625070083083 20.10441319742931 28-29 20.40277125196353 29.75378785539697 29.59288634731924 20.250554545320263 30-31 20.207934519582064 29.635181228727653 29.7671264270697 20.389757824620578 32-33 20.43210579223686 29.86305026380112 29.6105073215137 20.094336622448317 34-35 20.320815704583627 29.580819959837402 29.72540868145869 20.372955654120286 36-37 20.276896380745143 29.78136625511883 29.75966116287836 20.18207620125767 38-39 20.293706757018157 29.764071496700062 29.76795028613087 20.174271460150916 40-41 20.177820768077197 29.684212194305587 29.92631763746911 20.211649400148104 42-43 20.193293828373633 29.702551330409086 29.89538311882533 20.20877172239195 44-45 20.172629543241474 29.595525843646588 29.868868869170445 20.36297574394149 46-47 20.112296888155175 29.603222738903927 30.039980107042634 20.244500265898264 48-49 20.168679495924714 29.766098318118477 29.900712864212476 20.16450932174433 50-51 20.050577494533773 29.7273116549089 30.02242829141389 20.19968255914344 52-53 20.109991768610502 29.597475423269966 30.126596012338 20.16593679578154 54-55 20.11361769316188 29.697239415048678 30.020395877032303 20.168747014757145 56-57 20.070003713842215 29.710600816234656 29.93312834993721 20.286267119985922 58-59 20.09610929867507 29.71330829658903 29.912987169010048 20.277595235725855 60-61 19.98294234806311 29.62627704980534 30.09416215408126 20.296618448050292 62-63 19.957153285359563 29.746401901760215 30.00884557913888 20.287599233741332 64-65 20.054977351517792 29.59447242201666 30.04202148829172 20.308528738173827 66-67 20.008659507138525 29.53992522199148 30.01224696923057 20.439168301639423 68-69 19.98431753983536 29.677392172067574 29.94097405498186 20.397316233115205 70-71 20.099685729316157 29.552409946937047 29.94393879527322 20.40396552847358 72-73 20.01777983649696 29.59197196478709 29.93036794710189 20.459880251614067 74-75 19.92544820157626 29.626272161073818 29.991954273197408 20.456325364152512 76-77 19.981935135092396 29.566839061261526 29.995101430483107 20.456124373162975 78-79 20.06901335408535 29.531978399920206 29.945290961332542 20.4537172846619 80-81 19.968041989806864 29.59582495869806 29.91888155211782 20.517251499377256 82-83 20.025139211024985 29.58719317554972 29.98002974981829 20.407637863607 84-85 19.982017337306136 29.590506363461465 29.989763514302126 20.437712784930277 86-87 19.896121348227595 29.458908903466934 30.067439631282415 20.577530117023052 88-89 20.035325389778976 29.44181339794553 30.00963436792449 20.513226844351003 90-91 20.115555366353373 29.510368790303747 29.957819441390164 20.41625640195272 92-93 20.23566845668269 29.344635113399974 29.911456361500626 20.508240068416708 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 257187.0 1 135145.0 2 11684.0 3 9711.5 4 8666.0 5 7699.5 6 7055.5 7 7094.0 8 7737.5 9 8994.0 10 11223.0 11 14637.5 12 20072.5 13 28675.5 14 33850.0 15 41790.0 16 61574.0 17 91114.5 18 136715.5 19 204475.0 20 299574.5 21 429916.0 22 602060.0 23 820490.5 24 1079346.5 25 1382593.0 26 1742546.0 27 2138222.5 28 2548910.5 29 2758101.0 30 2953538.0 31 3328147.0 32 3661314.0 33 3949055.5 34 4194059.5 35 4384644.0 36 4493169.0 37 4517009.5 38 4473177.0 39 4375513.0 40 4242733.0 41 4069552.5 42 3875010.5 43 3780058.0 44 3661845.0 45 3393440.5 46 3118318.5 47 2858524.0 48 2600238.0 49 2356733.0 50 2121158.5 51 1911164.5 52 1715873.5 53 1526591.0 54 1362710.0 55 1217569.5 56 1077994.0 57 1008172.0 58 953310.0 59 848976.5 60 754375.5 61 667198.5 62 589710.0 63 519706.0 64 459253.5 65 417111.0 66 381460.0 67 338563.0 68 290950.5 69 249512.0 70 215875.5 71 199564.0 72 187127.5 73 162564.5 74 140926.5 75 124634.5 76 116741.0 77 114055.5 78 106706.5 79 98350.0 80 89164.0 81 79996.5 82 71303.5 83 57397.5 84 39715.0 85 24075.5 86 18344.0 87 14490.0 88 8244.0 89 4615.5 90 2650.5 91 1593.0 92 1031.0 93 644.0 94 402.5 95 275.0 96 207.0 97 161.0 98 116.0 99 102.5 100 116.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.5090098095929696 2 0.4996014622078353 3 0.5039043903159501 4 0.5051096040469186 5 0.4971949733528622 6 0.33661796743264366 7 0.346563934668357 8 0.359418563146947 9 0.35789136829667556 10-11 0.3430871293709306 12-13 0.31864413455266044 14-15 0.2939091904931874 16-17 0.3005496818344062 18-19 0.2880465740273487 20-21 0.3005024185508388 22-23 0.32181766711386267 24-25 0.3344566568611764 26-27 0.3414142060629931 28-29 0.342122170664763 30-31 0.33872216820313367 32-33 0.3491333833864635 34-35 0.33743522837766315 36-37 0.32626681600551405 38-39 0.336359988676505 40-41 0.32872302977340706 42-43 0.3286161950595099 44-45 0.31799278742599724 46-47 0.31400642485260993 48-49 0.3111268108360924 50-51 0.32506258692628653 52-53 0.36181077455167576 54-55 0.3842741270446909 56-57 0.39721097394365334 58-59 0.4060167144633033 60-61 0.41295309365268873 62-63 0.39639075904341087 64-65 0.3827592403411818 66-67 0.3796378943222519 68-69 0.3623459327729024 70-71 0.3510293303137347 72-73 0.3426912993710537 74-75 0.3504966337218605 76-77 0.37037675237239526 78-79 0.40120324442748656 80-81 0.4299821531871946 82-83 0.42445727226851454 84-85 0.4082986448730415 86-87 0.38982805518973007 88-89 0.3724612601080655 90-91 0.3563375878493975 92-93 9.942485999483058 >>END_MODULE >>Sequence Length Distribution pass #Length Count 93 1.0155875E8 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.59858063155951 #Duplication Level Percentage of deduplicated Percentage of total 1 84.30900699132586 54.462421860958784 2 10.389380796554528 13.422785061964074 3 2.42042218525299 4.690675130894424 4 0.8304435876463675 2.145819082261417 5 0.4673843774974725 1.5096183697850865 6 0.2929315253184819 1.1353776454687001 7 0.16831580985940695 0.7611073690338397 8 0.14728214780912932 0.7611374160669845 9 0.10731363362211795 0.623907757296362 >10 0.7407064576194077 9.326773086791306 >50 0.07579732796790178 3.3523809781298644 >100 0.04748772500669245 5.82760434808313 >500 0.002847396943151291 1.1911963798423808 >1k 6.695708966183394E-4 0.6681176200153033 >5k 2.865229498969348E-6 0.017805634798204475 >10k+ 7.601450429076286E-6 0.10327225861007064 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.846517409873596E-7 0.0 0.0 2 2.9539552229620787E-6 0.0 3.938606963949438E-6 3 3.938606963949438E-6 3.938606963949438E-6 7.877213927898876E-6 4 3.938606963949438E-6 6.8925621869115164E-6 2.067768656073455E-5 5 1.1815820891848315E-5 1.0831169150860955E-5 2.166233830172191E-5 6 1.7723731337772473E-5 1.3785124373823033E-5 2.264699004270927E-5 7 2.560094526567135E-5 1.5754427855797753E-5 2.8554900488633426E-5 8 3.7416766157519665E-5 1.6739079596785113E-5 3.0524203970608146E-5 9 4.13553731214691E-5 1.7723731337772473E-5 5.612514923627949E-5 10-11 6.104840794121629E-5 2.117001243122823E-5 9.452656713478652E-5 12-13 7.138725122158356E-5 2.707792287715239E-5 1.1618890543650842E-4 14-15 8.320307211343188E-5 3.101652984110182E-5 1.3982054722020505E-4 16-17 9.50188930052802E-5 3.692444028702598E-5 1.7822196511871208E-4 18-19 1.12250298472559E-4 4.086304725097542E-5 2.4468595763535883E-4 20-21 1.2948170393983777E-4 4.480165421492486E-5 3.1902716407990444E-4 22-23 1.4523613179563552E-4 4.9724912919861655E-5 4.401393282213497E-4 24-25 1.6049823378093961E-4 5.2678868142823735E-5 8.743707459967752E-4 26-27 1.7526800989574998E-4 5.809445271825421E-5 0.0023533176609597892 28-29 1.9545337058599088E-4 6.892562186911517E-5 0.011094563491575074 30-31 2.1908501236968752E-4 7.384888057405196E-5 0.021126687754624784 32-33 2.3287013674351054E-4 7.827981340849507E-5 0.033460927788102945 34-35 2.4911689046980197E-4 8.566470146590028E-5 0.05604785407461199 36-37 2.5945573375016925E-4 9.206493778231811E-5 0.10651322510369614 38-39 2.683175994190555E-4 9.600354474626755E-5 0.19392912969094245 40-41 2.8062574618139744E-4 9.846517409873596E-5 0.29513360493310525 42-43 2.934262188142332E-4 1.0092680345120435E-4 0.40079264465149483 44-45 3.0967297254052455E-4 1.0535773628564747E-4 0.5274513520499219 46-47 3.2247344517336024E-4 1.0781936563811586E-4 0.6811254569399485 48-49 3.4118182825212007E-4 1.0880401737910323E-4 0.8365207330732212 50-51 3.5792090784890517E-4 1.117579726020653E-4 0.9926579442933278 52-53 3.682597511292725E-4 1.1865053478897683E-4 1.1457712900168622 54-55 3.918913929129691E-4 1.2258914175292625E-4 1.307895183822172 56-57 4.061688431572858E-4 1.2603542284638202E-4 1.489624478442281 58-59 4.2635420384752663E-4 1.2751240045786306E-4 1.676209583123069 60-61 4.90356567011705E-4 1.3095868155131883E-4 1.85859859440964 62-63 5.061109948675028E-4 1.3194333329230617E-4 2.0336489962706317 64-65 5.2580402968725E-4 1.3538961438576194E-4 2.2077969648110085 66-67 5.386045023200857E-4 1.4228217657267345E-4 2.3969475796029394 68-69 5.499279973414403E-4 1.4917473875958496E-4 2.593706106071609 70-71 5.676517286792127E-4 1.5705195268748384E-4 2.7859682203650595 72-73 5.799598754415548E-4 1.5951358203995225E-4 2.9727645328442893 74-75 5.907910445924157E-4 1.6295986313340802E-4 3.153703152116386 76-77 6.006375620022893E-4 1.6689847009735743E-4 3.34255787906015 78-79 6.158996639875934E-4 1.7083707706130687E-4 3.540108557854444 80-81 6.252538555269734E-4 1.7576033576624368E-4 3.7307130109419426 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACTG 42230 0.0 16.22016 5 CTATACT 50115 0.0 14.188535 4 GTGTAGG 34770 0.0 14.130824 1 TATACAG 41545 0.0 13.972718 5 GTATATA 85685 0.0 13.684979 1 GTATAAG 46025 0.0 13.662068 1 TACACTG 31035 0.0 13.577932 5 GTATTAG 44860 0.0 13.3788595 1 TACACCG 17615 0.0 13.339384 5 GTATAGG 36780 0.0 13.122775 1 TATACCG 16850 0.0 13.121679 5 GTATAGA 51970 0.0 13.067163 1 TCTATAC 51635 0.0 12.873346 3 CTTACAC 31765 0.0 12.831354 3 GTATAAT 68710 0.0 12.742618 1 CAGCGCG 10615 0.0 12.561827 9 GTGTAAG 33125 0.0 12.5022955 1 TTATACT 74775 0.0 12.106972 4 ACTGCGC 10050 0.0 12.105326 8 GTGTTAG 28080 0.0 12.092244 1 >>END_MODULE