FastQCFastQC Report
Wed 5 Nov 2014
lane8_Undetermined_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane8_Undetermined_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101558750
Sequences flagged as poor quality0
Sequence length93
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG432550.016.5753695
CTATACT511500.014.6271314
TATACAG425300.014.01429755
TACACTG313600.013.7257865
CTTACAC318750.013.2184373
TACACCG177250.013.2060085
GTGTAGG324600.013.1327611
GTATATA857100.013.0772921
GTATTAG444550.012.9483981
ACTGTGC278300.012.9435048
TATACCG170950.012.9066255
TCTATAC523950.012.7572943
GTATAAG458600.012.7315511
GTATAGG364700.012.6529151
TTATACT754250.012.6263784
GTATAGA506700.012.34542751
ACTGCGC107800.012.2643658
TAGACTG298950.012.2089295
TAATACT654650.012.1305464
CCTATAC376250.012.0048083
Produced by FastQC (version 0.11.2)