FastQCFastQC Report
Wed 5 Nov 2014
lane3_Undetermined_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane3_Undetermined_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5876217
Sequences flagged as poor quality0
Sequence length93
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN649851.1058985738613807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG24200.014.823455
GTATTAG23000.013.9256391
GTCTGGG24050.013.8575651
GTATAGA27550.013.6681241
TACACCG10900.013.4815235
TTATACT39200.013.0108324
TACACTG18750.012.9084265
ATACCGT11300.012.6093746
GTGTAGG21750.012.337971
TATACCG9650.012.0926935
GTATAAG22950.012.0700351
CTACACT20950.011.9661124
GTCTTAG20550.011.7947151
GTCCTAT21300.011.7858161
TAGTACT20600.011.7497854
CTAGTAC15900.011.6909053
ATACAGT24350.011.5258366
TTACACC18900.011.4345044
ATTATAC32200.011.4114253
CTGTCCG7951.8189894E-1211.403329
Produced by FastQC (version 0.11.2)