FastQCFastQC Report
Wed 5 Nov 2014
lane2_Undetermined_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane2_Undetermined_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5754556
Sequences flagged as poor quality0
Sequence length93
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN884511.5370603744233264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG24650.015.2408075
GTATTAG24550.013.9263141
GTATACG7050.013.5049871
GCGCTTA11350.013.3454361
GTATATA50850.013.2768251
ACAGTGC14650.013.2520468
GTATAAG25250.013.1974471
GTATAGG19600.013.0273631
TACACCG11350.012.9356635
CTACACC18450.012.8741434
CTGCGCG7550.012.5659349
TAAGCGC11950.012.2763486
GTCTGGG21350.012.1622511
CTACACT21650.011.7691774
ACCGTGC9550.011.7456768
TATACAG22900.011.6912755
ACACCCG14800.011.6708885
ATTACGC5651.6745616E-811.4710573
CACCCGG14700.011.4473646
GTATAAT36750.011.4228631
Produced by FastQC (version 0.11.2)