FastQCFastQC Report
Wed 5 Nov 2014
DP6A_TAGGCATG-TAGATCGC_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDP6A_TAGGCATG-TAGATCGC_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53830827
Sequences flagged as poor quality0
Sequence length93
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1051880.195404763148075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG234050.015.7474485
CTATACT282500.014.7746834
GTATATA475750.014.0578121
TATACAG230200.013.9668835
GTATAAG252500.013.8043771
GTATTAG247600.013.6552371
GTATAGA291600.013.53721
TCTATAC280600.013.5239023
TACACCG85000.013.2235855
TACACTG158400.012.9268035
GTGTAGG185500.012.8948581
GTATTAT400650.012.5169451
CCTATAC204100.012.1889043
GTATAAT366100.012.0915551
GTATAGG209850.012.0837621
TATACCG93950.011.9638615
ATAATAC384800.011.7525973
TTATACT405400.011.7142334
ATTATAC358950.011.7129123
CTTACAC159850.011.6382263
Produced by FastQC (version 0.11.2)