FastQCFastQC Report
Wed 5 Nov 2014
DP18_GGACTCCT-CTCTCTAT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDP18_GGACTCCT-CTCTCTAT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42852012
Sequences flagged as poor quality0
Sequence length93
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG183750.016.514675
TACACCG79600.015.6106075
CTATACT218800.014.3080664
GTGTAGG149500.014.2036251
GTATAAG202400.014.0458661
TATACAG179800.014.0403165
TATACCG68400.013.8959555
GTATTAG199650.013.7807061
GTATAGG145450.013.6698131
GTACTAG131550.013.3906591
GTATATA390300.013.2717851
TACACTG125550.013.2658385
TCTATAC236450.013.1479673
GTGTAAG140750.012.9181141
GTATAGA234150.012.7744261
CTTACAC136300.012.7639593
GTATAAT307900.012.5185491
ACTGTGC117950.012.4289078
ACACCGT69850.012.2712766
TTATACT339500.012.2264674
Produced by FastQC (version 0.11.2)