FastQCFastQC Report
Wed 5 Nov 2014
lane8_Undetermined_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane8_Undetermined_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101558750
Sequences flagged as poor quality0
Sequence length93
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG422300.016.220165
CTATACT501150.014.1885354
GTGTAGG347700.014.1308241
TATACAG415450.013.9727185
GTATATA856850.013.6849791
GTATAAG460250.013.6620681
TACACTG310350.013.5779325
GTATTAG448600.013.37885951
TACACCG176150.013.3393845
GTATAGG367800.013.1227751
TATACCG168500.013.1216795
GTATAGA519700.013.0671631
TCTATAC516350.012.8733463
CTTACAC317650.012.8313543
GTATAAT687100.012.7426181
CAGCGCG106150.012.5618279
GTGTAAG331250.012.50229551
TTATACT747750.012.1069724
ACTGCGC100500.012.1053268
GTGTTAG280800.012.0922441
Produced by FastQC (version 0.11.2)